Influence of Panamin Animal and Panamin Detox on milk coagulation properties of dairy cows

Abstract. The aim of the study was to evaluate the differences in milk coagulation properties in three groups of dairy cows receiving the dietary supplements Panamin Animal and Panamin Detox. The present group production experiment was conducted in the experimental cattle farm of the Agricultural Institute – Stara Zagora with three groups (two experimental and one control) each comprising 11 lactating dairy cows between November 2016 and March 2017. The cows are kept freely, the milking is in a hall “Fish bone”, and the groups were fed balanced rations compliant with milk yield and live body weight of cows, supplemented with 0.50 g/day of two natural dietary additives – Panamin Animal and Panamin Detox in line with recommendations of the manufacturer. The following parameters were monitored: rennet coagulation time (min) – RCT and curd firmness (mm) – a30. The analysis of individual milk coagulation properties was done on 436 milk samples from cows. Individual milk samples were obtained during the morning milking without adding preservative. The analysis of milk coagulation ability was evaluated at the laboratory of the Agricultural Institute – Stara Zagora by means of Computerized Renneting Metter – Polo Trade, Italy. The obtained phenotypes were corrected for main factors influencing test-day milk yield. The used model considered each test day milk yield as independent observation and unbiased estimates of traits were obtained by a linear mixed-effects model. The supplementation of cows’ ration with dietary supplements Panamin Detox and Panamin Animal had a statistically significant beneficial effect on milk coagulation properties of milk (p<0.001). The statistical analysis of group differences in RCT and curd firmness showed highly significant differences between Panamin Animal and control groups for both traits (p<0.001). There was also a highly relevant difference in curd firmness of milk from cows given Panamin Detox supplement and control cows (p<0.001). Data were processed with statistical software products Systat 13 and Pest (Groeneveld), and graphs were generated in MS Excel.

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Relationships between milk yield, quality and coagulation properties with kappa-casein (к-CN) genotypes of Bulgarian Rhodope cattle breed

Agricultural Science and Technology ◽

10.15547/ast.2019.03.034 ◽

2019 ◽

Vol 11 (3) ◽

pp. 203-206

Author(s):

I. Mehandzhiyski ◽

Т. Angelova ◽

D. Yordanova ◽

J. Krastanov

Keyword(s):

Dairy Cows ◽

Protein Content ◽

Milk Yield ◽

Tissue Sample ◽

Milk Proteins ◽

Coagulation Time ◽

Tissue Samples ◽

Milk Samples ◽

Rennet Coagulation ◽

Yield Quality

Abstract. The aim of the present study was to establish the association between different к-CN genotypes with milk yield, quality and coagulation properties in dairy cows of Bulgarian Rhodope breed. For determination of milk proteins polymorphism and its relationship with individual coagulation properties of milk and its quality, 136 milk samples from Bulgarian Rhodope dairy cows reared in 4 farms were collected. The visit to the farms and the taking of the milk samples took place between May and June 2009. Animal milk is based on daily milking on the relevant control day of the current lactation. During the morning milking, 50mL samples were collected without preservative, stored in cooling bags and analyzed in the specialized lab at the Agricultural Institute, Stara Zagora by Computerized Renneting Metter. The evaluated parameters of milk coagulation properties were: rennet coagulation time (RCT, min); curd firming time (K20, min) and curd firmness (A30, mm). Milk proteins polymorphism was identified by tissue samples collection from 136 cows. To this end, specialized pliers and marks with a vial containing desiccant were used. With this technique, the tissue sample is obtained and sealed at the time of identification of the animal. After collection, tissue samples were shipped to the University in Padova, Italy for PCR-RFLP analysis. DNA was purified using a Maxwell®16 Tissue DNA purification kit (Promega) according to the manufacturer’s instructions. Data were analyzed using descriptive statistical analysis (SYSTAT 13). The results obtained give reason to the following conclusions: The highest milk yield was established in cows from the AH genotype – 16kg, whereas the lowest value was detected in cows from the BH genotype – 12.83kg. Homozygous cows from the BB genotype produced milk with the highest fat content – 5.11%, whereas heterozygous from the BH genotype: milk with the lowest protein content – 3.16%. The cows from the AH genotypes were outlined with the lowest milk fat and protein content. The longest rennet coagulation time (RCT) was observed in АА and АН genotypes, the longest curd firming time (К20) – in genotype AH, the highest curd firmness (А30) – in the milk of cows from the AB and BB genotypes.

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Milk protein genotypes and milk coagulation properties of Estonian Native cattle

Agricultural and Food Science ◽

10.2137/145960607783328209 ◽

2008 ◽

Vol 16 (3) ◽

pp. 222 ◽

Cited By ~ 15

Author(s):

I. JÕUDU ◽

M. HENNO ◽

S. VÄRV

Keyword(s):

Genetic Variation ◽

Milk Protein ◽

Coagulation Parameters ◽

Milk Samples ◽

Cattle Breeds ◽

Rennet Coagulation ◽

Native Cattle ◽

Milk Coagulation

The genetic variation of ás1-, â- and ê-caseins and b-lactoglobulin was determined and their effects on the rennet coagulation properties were examined using 335 milk samples from 118 Estonian Native (EN) cows. We found 16 aggregate casein genotypes (ás1-, â-, ê-caseins), of which four . namely, BB A2A2 AA (21.2%), BB A1A2 AB (16.9%), BB A1A2 AA (14.4%), and BB A2A2 AB (10.2%) occurred among nearly two-thirds of the analysed cows. Aggregate casein genotype had a significant overall effect on rennet coagulation parameters. Better rennet coagulation properties were found for aggregate casein genotypes CC A2A2 AB and BC A1A2 BB, among frequent genotypes for BB A1A2 AB. Of the cattle breeds raised in Estonia, milk from EN had the best coagulation properties and highest frequency of favourable ê-Cn B allele.;

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RELATIONSHIP BETWEEN BLOOD METABOLIC PROFILES AND MILK YIELD ASSOCIATED WITH PARITY AND STAGE OF LACTATION IN CROSSBRED DAIRY COWS IN BANGLADESH

Journal of Veterinary Medical and One Health Research ◽

10.36111/jvmohr.2019.1(2).0011 ◽

2019 ◽

Vol 1 (2) ◽

Author(s):

L Naher ◽

M. A. Samad ◽

S. H. M. F. Siddiki ◽

M. T. Islam

Keyword(s):

Dairy Cows ◽

Milk Yield ◽

Metabolic Profile ◽

Transition Period ◽

Dairy Herds ◽

Metabolic Profiles ◽

Milk Samples ◽

Stage Of Lactation ◽

Lactating Dairy Cows ◽

Calcium Phosphorus

Background: Cattle cross-breeding program has been launched throughout Bangladesh but this program has still focused mainly on biological rather than economic evaluation. Currently, 30000 dairy farms with mainly cross-bred cows are in operation throughout the country and this intensive dairy farming system with high milk yielding dairy cows is supposed to be associated with high incidence of sub-clinical nutritional and metabolic diseases. Objectives: The objective of this study was to detect the relationship between major blood metabolic profiles and milk yield associated with parity and stage of lactation in cross-bred dairy cows. Materials and Methods: Blood samples of 220 apparently healthy lactating cross-bred dairy cows (HF x L, n = 190; SH x L, n = 20 and JS x L, n = 10) of 10 dairy herds were collected for metabolic profile test (MPT) in Bangladesh during the period from July to November 2016. The major metabolic profiles which are associated with milk fever (calcium, phosphorus & magnesium) and ketosis (glucose) were considered in this study. These biochemical parameters were estimated to detect the influence of risk factors including herds, breeds, parities and lactation age of cross-bred lactating dairy cows by using the commercial kits in spectrophotometer method. Results: The evaluation of the biochemical constituents of 220 cross-bred lactating dairy cows revealed that 30% (n = 66) had hypocalcaemia and 20.45% (n = 45) had hypoglycemia. Significantly lower levels of average calcium (7.93 ± 0.36 mg/dl; p < 0.024) and glucose (43.44 ± 3.63 mg/dl; p < 0.0001) values were recorded in HF x L cross-bred dairy herd of BAUDF, Mymensingh in comparison to other investigated nine dairy herds. The highest milk yield was recorded at 7th parity (16 +- 0.91 liter / day) and 1st week of lactation (17.33 +- 1.09 liter / day) were associated with low mean calcium (8.09 +- 0.85 mg / dl; 6.68 +- 0.13 mg/dl) and glucose (36.45 +- 7.67 mg/dl; 32.31 +- 3.90 mg / dl) levels in comparison to the respective values of different parity and lactation weeks. Conclusions: A relationship between blood metabolic profiles and milk yield associated with herd, breed, parity and lactation stages was recorded in lactating crossbred dairy cows. The evaluation of blood metabolites at different stages of lactation cycle especially at transition period is required to detect the nutritional and metabolic health for optimum milk production and to achieve maximum reproductive potential of high yielding dairy cattle. The readily available milk samples could be used as a biological fluid to monitor the health and nutritional status of dairy cows by using mid-infrared (MIR) spectroscopy method to prevent sub-clinical metabolic disorders. Therefore, well developed laboratories with necessary equipment, test kits and reagent should be provided to perform MPT in both the blood and milk samples for practical uses. Keywords: Compton metabolic profile test, Metabolic profile, Lactating cross-bred cows, Calcium, Phosphorus, Magnesium, Glucose, Parity, Lactation stages

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Effect of feeding hay vs. silages of various types to dairy cows on feed intake, milk composition and coagulation properties

Journal of Dairy Research ◽

10.1017/s0022029920000801 ◽

2020 ◽

Vol 87 (3) ◽

pp. 334-340

Author(s):

Elisa Manzocchi ◽

Werner Hengartner ◽

Michael Kreuzer ◽

Katrin Giller

Keyword(s):

Dairy Cows ◽

Milk Yield ◽

Feed Intake ◽

Milk Fat ◽

Fixed Effects ◽

Experimental Period ◽

Milk Composition ◽

Processing Technology ◽

Maize Silage ◽

Rennet Coagulation

AbstractThis research paper addresses the hypotheses (1) that milk produced from hay-fed cows differs from that of silage-fed cows and (2) that silage type has an important impact, too. Four diets differing in forage type but with equal estimated milk production potential and a forage:concentrate ratio of 0.85 : 0.15 were compared regarding their effect on feed intake, milk yield and milk properties. The forages tested were hay, grass silage, conventional short-chopped and long-chopped maize silage subjected to a novel processing technology (Shredlage®). Twenty-four dairy cows were fed two of the four diets in two consecutive runs in an incomplete (4 × 2) Latin-square design (n = 12 per diet). Each experimental period lasted 22 d, with 12 d of adaptation and 10 d of sampling. During sampling, feed intake and milk yield were recorded daily, milk composition and coagulation properties were determined four times. The composition of the diet ingredients was analysed weekly. Data were analysed with a mixed model considering feed, period and their interaction as fixed effects. Stage of lactation, milk yield and milk composition from the pre-experimental period were used as covariates in the model. Dry matter intake was lower with the long-chopped processed maize silage compared to the other three groups. There were some diet differences in intakes of net energy for lactation and absorbable protein in the duodenum, but this did not result in changes in milk yield. The milk fat content was higher with the grassland-based diets compared to the maize silage diets. No treatment effect on milk acidity and rennet coagulation properties was observed. In conclusion, there were no indications for specific physico-chemical properties of milk from a hay-based diet, and maize processing technology was not of large effect either. Future investigations should focus on sensory differentiation of the milk produced with different forages.

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The effects of raising post-oestrus progesterone concentrations on luteal activity in post-partum dairy cows

Animal Science ◽

10.1017/s1357729800050554 ◽

1999 ◽

Vol 68 (3) ◽

pp. 527-532 ◽

Cited By ~ 8

Author(s):

A. O. Darwash ◽

G. L. Ward ◽

G. E. Lamming ◽

J. A. Woolliams

Keyword(s):

Dairy Cows ◽

Untreated Control ◽

Luteal Phase ◽

Post Partum ◽

Milk Samples ◽

Holstein Friesian ◽

The Mean ◽

And Control ◽

Phase Length ◽

Friesian Cows

AbstractIn order to investigate the rationale of manipulating post-ovulation progesterone (P4) concentrations, luteal activity was measured in 10 Holstein-Friesian cows treated with a progesterone-releasing device (CIDR, 1·9 g P4) inserted on the morning of day 2 post-oestrus for a period of 7 days and compared with 10 untreated control animals. Milk samples were collected daily during afternoon milking from 7 days pre- to 24 days post-oestrus and P4 concentrations were measured using enzyme-linked immuno-sorbent assay (ELISA). The milk P4 profiles were used to assess the effects of early post-oestrus P4 elevation on five intervals of the luteal phase. In the treated (T) and control (C) animals, the mean interval from oestrus to P4 rise (≥3 μg/l), was 2·38 (s.e. 0·18) and 4·90 (s.e. 0·28) days, respectively, (P < 0·01). The mean interval from oestrus to the attainment of peak P4 concentrations was not significantly different (P > 0·05) between the T (14·75 (s.e. 1-62) days) and С (14·30 (s.e. 0·70) days) animals, with cows in the T group showing a more variable (F7 9 = 4-30, P < 0·05) interval to the occurrence of the peak. The interval from peak P4 to corpus luteum (CL) regression (when P4 fell below ≥3 μg/l) was not significantly different between the T (4·13 (s.e. 1·30 days) and С (5·60 (s.e. 0·88) days) groups. Furthermore, CIDR insertion did not alter luteal phase length, number of days with P4 concentrations ≥3 μg/l (T = 16·50 (s.e. 0·80) v.C = 15·00 (s.e. 0·47) days) or the interval from oestrus to CL regression (T = 18·88 (s.e. 0·79) v.C = 19·90 (s.e. 0·41) days). We conclude that treatment with CIDR 2 days after oestrus successfully increased the availability of P4 to cows without significantly affecting luteal phase characteristics.

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Effects of Mycotoxin Sequestering Agents Added Into Feed on Health, Reproduction and Milk Yield of Dairy Cattle

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun201462030495 ◽

2014 ◽

Vol 62 (3) ◽

pp. 495-500

Author(s):

Michal Hulík ◽

Ladislav Zeman

Keyword(s):

Dairy Cattle ◽

Dairy Cows ◽

Pregnancy Rate ◽

Milk Yield ◽

The European Union ◽

Negative Effects ◽

Control Groups ◽

Agent Based ◽

And Control ◽

Positive Effect

Effects of mycotoxin sequestering agents in feed on health, reproduction and milk yield of dairy cattle were studied in a 5-month long experiment on 300 dairy cows divided into two groups and six subgroups. The experiment was conducted in adding a mycotoxin sequestering agent based on 1,3 and 1,6 β-glucans to standard cattle nutrition (TMR), which was regularly tested for content of important mycotoxins, in order to gain knowledge about possible positive effect of this agent on the health of dairy cattle and about possible avoidance of negative effects of mycotoxins on dairy cattle due to their structural elimination caused by the agent. The experiment’s setting and conditions during it were in all aspects common and comparable within the European Union, the experiment’s results should be therefore seen as relevant. Health, pregnancy rate and milk yield were carefully monitored during the experiment. Indicators of state of health (occurrence of mastitis and somatic cell count in milk) did not show any significant differences between test and control groups of dairy cows. The average milk yield of dairy cows which were fed the agent enriched feed (30.2 kg a day) was slightly lower in comparison to control groups (31 kg a day, both results with P < 0.001), however, fat content of milk of test groups’ cows (4.02%) was considerably higher than that of control groups’ cows (3.79%). The average pregnancy rate of cows which were fed the agent enriched feed also manifested considerable increase in percentage and stability (from 42.95% of control groups’ cows to 62.25% of test groups’ cows, the standard deviation decreased from 21.1% to 14.4% which means smaller differences among pregnancy rate of test groups’ cows, hence higher stability), this increase manifested even long after the cows had been fed regular feed again.

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Effect of replacing maize grain by hybrid rye grain in the TMR on performance of mid-lactating dairy cows

Annals of Animal Science ◽

10.2478/aoas-2021-0006 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Patrycja Rajtar ◽

Marek Sady ◽

Paweł Górka ◽

Sylvia Kehoe ◽

Piotr Micek

Keyword(s):

Dairy Cattle ◽

Dairy Cows ◽

Milk Yield ◽

Milk Fat ◽

Dry Matter ◽

Maize Silage ◽

Rennet Coagulation ◽

Cereal Grain ◽

Hybrid Rye ◽

Maize Grain

Abstract Grain from traditional varieties of rye is not commonly used in dairy cattle nutrition. However, new hybrid varieties of rye currently available are characterized by some nutritional and agrotechnical benefits. This paper deals with the hypothesis that rye grain derived from a hybrid variety may be an alternative for maize grain in diets for dairy cattle. Sixteen lactating Polish Holstein-Friesian cows were divided into two groups according to their parity (8 primi- and 8 multiparous), stage of lactation (106 ± 30 days after calving) and milk yield (34 ± 4 kg/day). Cows were fed a total mixed ration (TMR) containing grass silage and whole crop maize silage and 29.2% of the concentrate (in dry matter). The latter contained approximately 48% of either maize grain (M) or hybrid rye grain (HR) as a main source of cereal grain. Experimental diets were fed for 9-weeks. The use of HR as a substitute for M did not affect (P>0.05) dry matter intake and milk yield. There were no differences between treatment groups in the content of milk solids, amino acids, and fatty acid profile. However, substituting M by HR positively influenced composition of milk protein fractions by increasing the proportion of α-casein (37.0 vs 39.7%; P<0.01) and к-casein (6.5 vs 7.3%; P=0.02) as well as decreasing the proportion of β-casein (28.8 vs 27.8%; P=0.02) and sensory characteristics of the milk (body and texture and taste; P<0.05). In turn, the composition of the diet did not affect the technological suitability for processing of milk fat (acid and peroxide number, melting and solidification temperature), rennet coagulation time, heat stability or titratable acidity. This study has shown that hybrid rye grain may be an alternative for maize grain in a TMR based on grass and whole maize silage for mid-lactation dairy cows. Further studies are needed with higher proportion of hybrid rye grain in TMR or with other roughages used in a basal diet to fully determine efficiency of hybrid rye grain use in diets for dairy cows.

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Variation of milk coagulation properties, cheese yield, and nutrients recovery in curd of cows of different breeds before, during and after transhumance to highland summer pastures

Journal of Dairy Research ◽

10.1017/s0022029916000583 ◽

2016 ◽

Vol 84 (1) ◽

pp. 39-48 ◽

Cited By ~ 9

Author(s):

Francesco Zendri ◽

Maurizio Ramanzin ◽

Claudio Cipolat-Gotet ◽

Enrico Sturaro

Keyword(s):

Dairy Cows ◽

Favourable Effect ◽

Cheese Making ◽

Brown Swiss ◽

Concentrate Supplementation ◽

Milk Samples ◽

Before And After ◽

Nutrients Recovery ◽

Milk Coagulation ◽

The Individual

This paper aimed at evaluating the effect of summer transhumance to mountain pastures of dairy cows of different breeds on cheese-making ability of milk. Data were from 649 dairy cows of specialized (Holstein Friesian and Brown Swiss) dual purpose (Simmental) and local (mostly Rendena and Alpine Grey) breeds. The Fourier-Transform Infra-Red Spectra (FTIRS) of their milk samples were collected before and after transhumance in 109 permanent dairy farms, and during transhumance in 14 summer farms (with multi-breeds herds) of the Trento Province, north-eastern Italy. A variety of 18 traits describing milk coagulation, curd firming, cheese yield and nutrients recovery in curd/loss in whey were predicted on the basis of FTIRS collected at the individual cow level. Moving the cows to summer farms improved curd firming traits but reduced cheese yields because of an increase of water and fat lost in the whey. During summer grazing, most of cheese-making traits improved, often non-linearly. The milk from summer farms supplementing cows with more concentrates showed better curd firming and cheese yield, because of lower fat lost in the whey. The breed of cows affected almost all the traits with a worst cheese-making ability for milk samples of Holsteins through all the trial, and interacted with concentrate supplementation because increasing compound feed tended to improve cheese-making traits for all breed, with the exception of local breeds for coagulation time and of Brown Swiss for curd firming time. In general, summer transhumance caused a favourable effect on cheese-making aptitude of milk, even though with some difference according to parity, initial days in milk, breed and concentrate supplementation of cows.

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Factors affecting raw milk quality of dairy cows under practical conditions

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1336 ◽

2020 ◽

Vol 14 ◽

pp. 744-749

Author(s):

Vladimír Tančin ◽

Šimon Mikláš ◽

Maroš Čobirka ◽

Michal Uhrinčať ◽

Lucia Mačuhová

Keyword(s):

Dairy Cows ◽

Milk Yield ◽

Dairy Farm ◽

Raw Milk ◽

Factors Affecting ◽

Milk Samples ◽

Stage Of Lactation ◽

Cell Counts ◽

Milk Components ◽

In The Beginning

Under the practical conditions, it is important to evaluate the factors affecting milk performance. Data from test day yield and milk components should be useful for such evaluation. The aim of the experiment was to study the effect of season, udder health (by somatic cell counts SCC), parity, stage of lactation on milk production, milk components, and SCC under the practical conditions. Also, the frequency of incidence of high SCC during the season was observed. The experiment was realized on one dairy farm in dairy practice. The experiment lasted from December 2015 to October 2017. We examined 481 Holstein dairy cows (6910 milk samples). Milk samples were collected once per month – performed by recording test day. Only cows with 9 – 11 test days were evaluated. The effect of season, parity, stage of lactation, and SCC influenced most of the studied traits. The milk yield was highest at 2nd lactation. In the following lactations, the milk yields were decreasing. The SCC significantly increased with advanced parity. The elevated SCC was found in the beginning and in the final part of lactation. SCC as a factor significantly reduced milk yield, lactose content but increased fat and protein content. In conclusion, under practical conditions, the management should use the data from test days and analyze them for a better understanding of the performance efficiency at the farm level and for implementing more sophisticated decision making in farming.

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Effects of dairy factory, milk casein content and titratable acidity on coagulation properties in Trentingrana dairy industry

Journal of Dairy Research ◽

10.1017/s0022029916000212 ◽

2016 ◽

Vol 83 (2) ◽

pp. 242-248 ◽

Cited By ~ 5

Author(s):

Mauro Penasa ◽

Valentina Toffanin ◽

Nicola Cologna ◽

Martino Cassandro ◽

Massimo De Marchi

Keyword(s):

Fixed Effects ◽

Titratable Acidity ◽

Testing Time ◽

Technological Properties ◽

Coagulation Time ◽

Milk Samples ◽

Rennet Coagulation ◽

Milk Coagulation ◽

Milk Casein

The objective of the present study was to investigate the effect of environmental factors, milk casein content and titratable acidity on milk coagulation properties (MCP) of samples routinely collected in the Trento province (northeast Italy) under field conditions. Rennet coagulation time (RCT, min), curd-firming time (k20, min) and curd firmness (a30, mm) were determined by Formagraph on 14 971 samples from 635 herds associated to 17 dairy factories. Besides MCP, fat, protein, and casein percentages, titratable acidity (TA), and somatic cell and bacterial counts were available. A standardised index of milk aptitude to coagulate (IAC) was derived using information of RCT and a30. An analysis of variance was conducted on MCP and IAC using a fixed effects linear model. Approximately 3% of milk samples did not form a curd within the testing time (30 min) and k20 was missing for 26% of milks. The percentage of samples without information on k20 largely differed among dairy factories (1·7–20·9%). Significant differences were estimated between the best and the worst dairy factory for RCT (−2 min), k20 (−1·2 min), a30 (+3·4 mm) and IAC (+2·6 points). Milk casein content and TA were important factors in explaining the variation of MCP and IAC, supporting the central role of these two traits on technological properties. The Trento province is heterogeneous in terms of dairy systems and this could explain the differences among dairy factories.

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