scholarly journals Mechanically deboned turkey meat with improved digestibility and biological value

2021 ◽  
Author(s):  
K. Takács ◽  
E. Szerdahelyi ◽  
A. Nagy ◽  
É. Gelencsér

AbstractEnzymatic hydrolysates of mechanically deboned meat (MDM) for a long time have been used as flavouring and functional food ingredients in the food industry and also as the bases of formula foods for special dietary uses.The aim of the present study was to produce MDM hypo-antigenic products with improved digestibility and high biological value to be used as a milk protein alternative. turkey MDM was treated with digestive enzymes (trypsin and/or α-chymotrypsin, or pancreatin), followed by freeze drying. The optimised reaction conditions of hydrolysis were at 6% (w/v) of meat protein in 0.1% NaHCO3 buffer, pH 7.5; pancreatin enzyme with 50 TAME units/g meat protein substrate, 37 °C and 60 min). Hydrolysates (MDMH) were assessed for degree of hydrolyses (DH, %) by using trinitrobenzenesulphonic acid method and MW distribution by SDS-PAGE. Modification of immune reactive binding sites in MDMHs was monitored by immunoblot with cow’s milk, chicken egg or meat allergic human patients’ sera. Biological value indices (True Digestibility (TD), Net Protein Utilisation (NPU), Biological Value (BV)) were determined using rat feeding trials. Among the MDMH products, the pancreatic hydrolysate proved to be the most favourable in terms of biological value and digestibility as well as hypoallergenic property.

1982 ◽  
Vol 62 (2) ◽  
pp. 537-545 ◽  
Author(s):  
L. D. CAMPBELL ◽  
B. O. EGGUM ◽  
J. WOLSTRUP

The influence of antibiotics (a 2:1 mixture of bacitracin and neomycin sulfate at 0.7% of the diet) on the availability of energy from barley-fishmeal-based diets was assessed using rats and adult roosters. Changes in estimates of protein quality, as influenced by dietary antibiotics were also determined in the study with rats. Gut biomass content was estimated by measuring the content of adenosine triphosphate (ATP) in gut samples of rats and fresh excreta samples of roosters. Antibiotic addition to the diet significantly (P < 0.01) reduced apparent digestible energy in rats and tended to reduce the true metabolizable energy measured with roosters. Biological value of the protein for rats was increased significantly (P < 0.01) by antibiotics as were net protein utilization values even though true digestibility of protein was decreased significantly (P < 0.01). The availability of some amino acids (e.g. histidine) showed a marked depression (12.1 percentage units) when antibiotics were added to the diet while others were not influenced (e.g. lysine and methionine) or markedly (7.6 percentage units) increased (e.g. alanine). The extent of microbial activity, which was shown to be markedly higher in the hindgut of rats fed no antibiotics as compared to rats fed antibiotics, may have contributed to the positive influence of antibiotic feeding on biological value of dietary protein and to the changes noted in excretion of amino acids by rats and roosters. Key words: Antibiotics, Nitrogen, amino acids, energy, rats, rosters


1964 ◽  
Vol 42 (3) ◽  
pp. 377-384 ◽  
Author(s):  
H. N. Parthasarathy ◽  
T. R. Doraiswamy ◽  
Myna Panemangalore ◽  
M. Narayana Rao ◽  
B. S. Chandrasekhar ◽  
...  

The true digestibility coefficient, biological value, and net available protein of diets based on processed soya flour supplemented with dl-methionine hydroxy analogue (MHA) or dl-methionine (at a level of 1.2 g/16 g N) have been determined in children aged 8–9 years. The mean daily intake of protein by the children on the different diets was maintained at a level of about 1.2 g/kg body weight. Supplementation of soya flour with dl-methionine brought about a marked increase in the biological value and net protein utilization of the proteins. MHA was, however, slightly less effective than dl-methionine in this respect. The biological value and net protein utilization of the different proteins were as follows: soya flour, 63.5 and 53.3; soya flour + MHA, 71.5 and 61.4; soya flour + methionine, 74.9 and 64.7; and skim milk powder, 82.6 and 72.0.


1964 ◽  
Vol 42 (3) ◽  
pp. 385-393 ◽  
Author(s):  
H. N. Parthasarathy ◽  
Kantha Joseph ◽  
V. A. Daniel ◽  
T. R. Doraiswamy ◽  
A. N. Sankaran ◽  
...  

The effect of supplementing a rice diet providing about 1.3–1.4 g protein per kg body weight with lysine, methionine, and threonine individually or together on true digestibility coefficient (DC), biological value (BV), and net protein utilization (NPU) of the proteins has been studied in girls aged 8–9 years. The retention of nitrogen on the rice diet was very low (9.5% of intake in the first series and 8.5% in the second series). The BV and NPU of the proteins of rice diet were 64.1 and 52.9 in the first series and 66.6 and 54.9 in the second series. Supplementation of the rice diet with lysine or methionine or lysine + methionine brought about a significant improvement in N retention (12.6, 12.0, 13.5% of intake) and in the BV (68.3, 66.3, 69.3) and NPU (54.8, 55.7, 55.8). When the rice diet was supplemented with lysine and threonine, a highly significant improvement in the N retention (18.9% of intake) and in the BV (77.4) and NPU (63.4) was observed. Addition of methionine to rice diet containing lysine and threonine resulted in a further improvement in N retention, BV, and NPU of the diets. The net available protein (g/kg body weight) from the different diets were as follows: rice diet, 0.71; rice diet + lysine, 0.76; rice diet + methionine, 0.74; rice diet + lysine + methionine, 0.77; rice diet + lysine + threonine, 0.85; rice diet + lysine + threonine + methionine, 0.91; and skim milk powder diet, 0.96–0.98.


2014 ◽  
Vol 44 (5) ◽  
pp. 464-470 ◽  
Author(s):  
Samson Ishola Ibironke ◽  
Mojisola Aderonke Adeniyi ◽  
Joseph Bandele Fashakin

Purpose – The purpose of this study is to nutritionally evaluate complementary food formulated from fermented maize, pigeon pea (Cajanus cajan L.) and soybean. Design/methodology/approach – Maize, pigeon pea and soybean were the sole energy and protein sources, respectively. Experimental diets consist of dietary Basal (A), fermented maize–soybean–pigeon pea 70:15:15 (B), fermented maize–soybean 70:30 (C), fermented maize–pigeon pea 70:30 (D) and Nutrend (E) a commercial dietary. A commercial product (Nutrend) manufactured by Nestle Plc was obtained at Orisunmibare central market, Oshogbo, Osun State, Nigeria, and was used as standard diet. The formulated complementary foods were fed to 30 albino rats for 28 days. Findings – The results showed that the average weight gained increased for dietary B, C, D and E (73.8-142.60, 73.8-98.26, 73.8-93.62 and 73.8-91.86, respectively), but diet A caused a decrease from 73.8 to 62.72 g. Protein qualities of dietary samples including biological value (BV; 94.26-98.67), true digestibility (TD; 72.55-80.46), net protein utilization (NPU; 71.72-76.53), protein efficiency ratio (1.63-3.49), feed efficiency (0.16-0.35), net protein ratio (2.49-4) and protein retention efficiency (46.38-61.24). Originality/value – Protein qualities of dietatry samples determined including biological value (BV), true digestibility (TD), net protein utilization (NPU) among others. The complementary food formulated from maize, pigeon pea and soybean supported animal growth and favourably compared with the standard. Meaning that, the application of multiple plant protein (pigeon pea and soybean) in the formulation of complementary food may be suitable for feeding of infants or children in developing countries to alleviate protein energy malnutrition.


Author(s):  
Н.А. БЕРЕЗИНА ◽  
А.В. АРТЕМОВ ◽  
И.А. НИКИТИН

Представлен комплексный подход к оптимизации состава мучной поликомпонентной смеси для ржано-пшеничных хлебобулочных изделий, учитывающий химический состав и технологические свойства пищевых ингредиентов смеси, а также их влияние на основной технологически формирующий компонент – ржано-пшеничную муку. Проектирование состава поликомпонентных смесей осуществляли с помощью разработанной ранее программы. Было сгенерировано более 20 образцов модельных мучных смесей, из которых отобрано 4 образца с показателями биологической ценности не менее 70% для приготовления хлебобулочных изделий. Приготовление осуществляли ускоренным способом с использованием подкислителя «Лезизауэр», прессованных дрожжей и соли в количестве, % от массы смеси, 2,0; 2,2 и 1,5 соответственно. Воду вносили по расчету с учетом массовой доли влаги ингредиентов. Продолжительность брожения составляла 60–70 мин, расстойки – 35–40 мин. Изделия выпекали при (200 ± 10)ºС до температуры внутри мякиша 97–98ºС. В качестве контроля был приобретенный в торговой сети хлеб ржано-пшеничный «Спасский», выпеченный АО «Орловский хлебокомбинат». Установлено, что выпеченные образцы ржано-пшеничного хлеба из разработанных мучных поликомпонентых смесей имеют сбалансированный состав белков, жиров и углеводов – 1,0 : 0,8 : 3,9–4,8 соответственно и кальция, фосфора и магния – 1,0 : 0,5–0,6 : 1,2–1,5 соответственно. По сравнению с хлебом ржано-пшеничным «Спасский» в хлебобулочных изделиях из мучных поликомпонентных смесей повысилось содержание белка и липидов соответственно на 0,8–2,3 и 2,7–3,5%, количество углеводов снизилось на 5,5–7,2%, увеличилось количество клетчатки в 4,5–5,5 раза, кальция в 5,5–7,5 раза, фосфора в 1,5–1,6 раза, магния в 1,1–1,2 раза. Биологическая ценность разработанных хлебобулочных изделий повысилась на 7,9–18,4% в сравнении с контрольным образцом. A comprehensive approach to optimizing the composition of a multi-component flour mixture for rye-wheat bakery products, taking into account the chemical composition and technological properties of the food ingredients of the mixture, as well as their influence on the main technologically forming component – rye-wheat flour, is presented. The design of the composition of multicomponent mixtures was carried out using a previously developed program. More than 20 samples of model flour mixtures were generated, of which 4 samples with indicators of biological value of at least 70% were selected for the preparation of bakery products. Preparation was carried out by an accelerated method using the acidifier «Lezisauer», compressed yeast and salt in an amount, % of the mass of the mixture, 2,0; 2,2 and 1,5 respectively. Water is added according to the calculation taking into account the mass fraction of moisture of the ingredients. The duration of fermentation was 60–70 minutes, proofing – 35–40 minutes. The products were baked at (200 ± 10)°C to the temperature inside the crumb 97–98°C. The rye-wheat bread «Spassky» purchased in the retail chain was used as a control. It was found that the baked samples of rye-wheat bread from the developed multi-component flour mixtures have a balanced composition of proteins, fats and carbohydrates – 1,0 : 0,8 : 3,9–4,8 respectively and calcium, phosphorus and magnesium – 1,0 : 0,5–0,6 : 1,2–1,5 accordingly. Compared with rye-wheat bread «Spassky» in bakery products made from flour multicomponent mixtures, the content of protein and lipids increased respectively by 0,8–2,3 and 2,7–3,5%, the amount of carbohydrates decreased by 5,5–7,2%, the amount of fiber increased by 4,5–5,5 times, calcium by 5,5–7,5 times, phosphorus by 1,5–1,6 times, magnesium by 1,1–1,2 times. The biological value of the developed bakery products increased by 7,9–18,4% in comparison with the control sample.


Author(s):  
Н.Н. АЛЕХИНА ◽  
Е.И. ПОНОМАРЕВА ◽  
И.М. ЖАРКОВА ◽  
К.К. ПОЛЯНСКИЙ ◽  
А.С. ЖЕЛТИКОВА

Проведена сравнительная оценка хлебопекарных смесей (ХПС) Ливенка и Амарантус, включающих биоактивированное зерно пшеницы и ржи и биоактивированные злаковые культуры и амарантовую муку соответственно, и хлеба на их основе. Исследованы химический состав, биологическая и энергетическая ценность ХПС и выпеченных на их основе образцов зернового хлеба Ливенский и Амарантус. Установлено, что ХПС Амарантус и хлеб Амарантус отличаются несколько большим содержанием белка, кальция, лизина по сравнению с ХПС Ливенка и хлебом Ливенский. Однако по количеству пищевых волокон и витаминов ХПС с амарантовой мукой и изделия на ее основе несколько уступают образцам, приготовленным без добавки амарантовой муки. Внесение амарантовой муки в дозировке 5,5% взамен части ржаной муки незначительно повышает энергетическую и биологическую ценность ХПС и хлеба на ее основе. Установлено, что ХПС Ливенка и Амарантус относятся к функциональным пищевым ингредиентам за счет содержания значимого количества биологически активных веществ, а изделия на их основе – к функциональным пищевым продуктам. Comparative evaluation of baking mixes (BM) of Livenka and Amarantus, including bioactivated grains of wheat and rye and bioactivated cereals and amaranth flour, respectively, and bread on their basis was carried out. Chemical composition, biological and energy value of BM and baked on their basis samples of grain bread Livenskiy and Amarantus were investigated. It is established that the BM Amarantus and bread Amarantus are slightly high content of protein, calcium, lysine, compared with BM Livenka and bread Livenskiy. However, the number of dietary fibers and vitamins BM with amaranth flour and products on it based are concede to samples a little prepared without the addition of amaranth flour. The introduction of amaranth flour in a dosage of 5,5% instead of a portion of rye flour slightly increases the energy and biological value of BM and bread on it based. It was found that the BM Livenka and Amarantus are functional food ingredients due to the content of a significant amount of biologically active substances, and products on their based – to functional foods.


1983 ◽  
Vol 50 (2) ◽  
pp. 471-478 ◽  
Author(s):  
E. Storm ◽  
E. R. Ørskov ◽  
R. Smart

Four experiments were conducted with eighteen lambs sustained entirely by intragastric nutrition at gross energy inputs varying from 430 to 860 kJ/kg live weight0·75 (W0·75). Isolated rumen micro-organisms (RMO) were infused into the abomasum in quantities varying from 0 to 2 g digestible N/kg W0·75 to assess the increase in N balance as a result of increasing RMO input when N was limiting.The over-all utilization of N from RMO (RMO-N) could be described by the equation y = 0·543 x −0·457, residual SD = 0·037, where y is the N balance and x is the abomasal input of RMO-N, both expressed in g/kg W0·75. Thus the coefficient of efficiency of utilization of infused RMO-N was 0·543 (SE 0·008). The coefficient of efficiency of utilization of RMO-N truly digested (i.e. the biological value) was 0·659 (SE 0·015).The RMO-N input (mean with SE) at N equilibrium was 0·843 (0·009) g/kg W0·75. The true digestibility of RMO-N was 0·813 (0·004). The urinary N excretion when no N was infused was 0·329 (0·008) g/kg W0·75 and the N excreted via the faeces with zero N input was 0·036 (0·009) g/kg W0·75.


1946 ◽  
Vol 14 (3) ◽  
pp. 330-339 ◽  
Author(s):  
Kathleen M. Henry ◽  
S. K. Kon

1. The biological value of proteins at an 8% level of protein intake measured on rats by the method of Mitchell (7, 10) was 52 for white bread of 73% extraction, 76 for Cheddar cheese, 75 for a mixture of both foods when each supplied 50% of the protein, and 67 when the two sources of protein were given on alternate days. The true digestibilities were, respectively, 99, 100, 98 and 99.2. Similar experiments with dried skim milk and dried cooked potatoes yielded biological values of 71 for potato, 89 for milk, 86 for the mixture and 81 for the substances fed separately on alternate days. The true digestibilities were, respectively, 82, 90, 82 and 87.3. An attempt to carry out experiments of the same type with dried whey and dried potato failed because of deaths of some rats receiving the whey diet, probably because of its high content of lactose. A biological value of 76 was obtained for the potato proteins and of 70 for a mixture of whey and potato in which whey supplied 25% of the protein. The true digestibilities were, respectively, 82 and 68.4. The biological value and the true digestibility of the proteins of a sample of edible soya-bean flour were, respectively, 73 and 90.5. It is concluded that there is a marked supplementary relationship between the proteins of milk and potato and those of bread and cheese when the members of each pair supply equal amounts of protein and when they are given together. No supplementary relationship is exhibited when the sources of protein are given separately on alternate days.


1984 ◽  
Vol 64 (3) ◽  
pp. 743-748 ◽  
Author(s):  
A. MAYLIN ◽  
A. CERVANTES ◽  
R. BEAMES

True protein digestibility and biological value of three sources of protein used in the feeding of pigs in Cuba were measured with rats. The sources were dried yeast (Candida utilis), cream yeast (C. utilis), and autoclaved garbage swill. The true protein digestibility of swill (84.4%) was similar to that of dried yeast. However, the true protein digestibility of cream yeast (96.3%) was significantly higher. Biological values of swill, dried yeast and cream yeast were 79.7, 72.3 and 76.6%, respectively, to produce respective net protein utilization (true protein digestibility × biological value) values of 67.4, 61.4 and 73.8%. The results support the present policy in Cuba of feeding yeast in the undried form. Key words: Rats, yeast, garbage, protein evaluation


1972 ◽  
Vol 27 (3) ◽  
pp. 461-466 ◽  
Author(s):  
J. Atkinson ◽  
P. Vohra ◽  
F. H. Kratzer

1. Chicks and quail (Coturnix japonica) were used in the measurement of net protein utilization and true digestibility of nitrogen of isolated soya-bean protein and a mixture of casein and gelatin in zinc-deficient diets.2. The net protein utilization values for both protein sources were increased when the diets were supplemented with Zn or with the disodium salt of ethylenediaminetetra-acetic acid, as was the true digestibility of nitrogen of the isolated soya-bean protein.


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