Production and fish identification of wheat- Aegilops biuncialis addition lines and their use for the selection of U and M genome-specific molecular (SSR) markers

2010 ◽  
Vol 58 (2) ◽  
pp. 151-158 ◽  
Author(s):  
A. Schneider ◽  
I. Molnár ◽  
M. Molnár-Láng
Keyword(s):  
Ssr Markers ◽  
Addition Lines ◽  
Wheat Genotype ◽  
Exact Identification ◽  
Translocation Lines ◽  
The Individual ◽  
High Level ◽  
M Genome ◽  
Selection Of

One way of incorporating useful traits from Aegilops biuncialis (2n=4x=28, U b U b M b M b ) into wheat ( Triticum aestivum L. 2n=6x=42, AABBDD) is to develop first addition then translocation lines. The 2M b , 3M b , 7M b , 3U b , 5U b and 5U b /6U b wheat- Ae. biuncialis addition lines were produced in Martonvásár. To facilitate the exact identification of the addition lines, it was necessary to analyse the fluorescence in situ hybridisation patterns of the parental wheat genotype, Ae. biuncialis and its diploid progenitors ( Ae. umbellulata 2n=2x=14, UU and Ae. comosa 2n=2x=14, MM). The great genetic variability of the Aegilops species causes polymorphism in the fluorescence in situ hybridisation (FISH) patterns of the individual chromosomes. Due to the high level of FISH polymorphism, it is advisable to confirm the identification of the Ae. biuncialis chromosomes with the help of molecular (microsatellite, SSR) markers, so 119 wheat SSR markers were tested on Aegilops biuncialis , on Ae. geniculata (2n=4x=28, U g U g M g M g ), on five wheat- Ae. biuncialis addition lines (2M b , 3M b , 7M b , 3U b , 5U b ) and on an addition series of wheat- Ae. geniculata in order to select SSR markers specific to the U and M genomes of Ae. biuncialis and Ae. geniculata .

Selection of U and M genome-specific wheat SSR markers using wheat–Aegilops biuncialis and wheat–Ae. geniculata addition lines

Euphytica ◽  
2010 ◽  
Vol 175 (3) ◽  
pp. 357-364 ◽  
Author(s):  
Annamária Schneider ◽  
István Molnár ◽  
Márta Molnár-Láng
Keyword(s):  
Ssr Markers ◽  
Addition Lines ◽  
M Genome ◽  
Selection Of

scholarly journals Basketball Technology Simulation Application Based on Virtual Reality

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Yushuai Song
Keyword(s):  
Virtual Reality ◽  
Great Influence ◽  
Physical And Mental Health ◽  
Basketball Players ◽  
Warm Up ◽  
Hit Rate ◽  
The Individual ◽  
High Level ◽  
Selection Of

The combination of virtual reality (VR) technology and basketball technology simulation can make the players have a real experience and experience, so as to effectively improve the quality of basketball training. It can also, according to the individual needs of different players, promote their physical and mental health development and improve the enthusiasm of basketball training, so as to improve the level of basketball technology. Immersive feeling and interest of human-computer interaction are the essential characteristics of virtual reality. The real conception of space-time environment, that is, the process of enlightening thinking and obtaining basketball technology simulation information, is the ultimate goal of virtual reality. In this experiment, five high-level basketball players in our city were selected and numbered from no. 1 to no. 5. Before the experiment, it is necessary to make the players warm up fully before the experiment and then test the forward turning technical movements of basketball. The test variables are based on different angles and distances. After that, standardized selection of basketball technical statistical indicators includes shooting hit rate, two-point hit rate, three-point hit rate, and free throw hit rate. According to the results of the experiment, it took the most time for the curve technique to lower the buffer stage, and it took up 29% of the total time of the entire precursor shot and turned back and the aerial shot stage is 23% and 22%, respectively. There is 20% time in the takeoff phase and 6% time in the brake phase. It has a great influence on the results of the game.

scholarly journals Thymus-independent positive and negative selection of T cells expressing a major histocompatibility complex class I restricted transgenic T cell receptor alpha/beta in the intestinal epithelium.

1993 ◽  
Vol 178 (6) ◽  
pp. 1947-1957 ◽  
Author(s):  
P Poussier ◽  
H S Teh ◽  
M Julius
Keyword(s):  
T Cell Receptor ◽  
Intestinal Epithelium ◽  
Transgenic Animals ◽  
Cell Receptor ◽  
Alpha Beta ◽  
High Level ◽  
Selection Of ◽  
Cell Receptor Alpha

We demonstrate that in the mouse intestinal epithelium the selection of T lymphocytes expressing a transgenic T cell receptor alpha/beta (TCR-alpha/beta) specific for male antigen (H-Y) in the context of H-2Db depends on the differential expression of H-Y and H-2Db in situ. In H-2Db transgenic males, there is no reduction in the number of intestinal intraepithelial lymphocytes (IEL), and the four main subsets of IEL expressing TCR-alpha/beta, defined by the differential expression of CD4, CD8 alpha, and CD8 beta, are present. Moreover, the level of expression of CD8 alpha and CD8 beta on CD8+ IEL subsets is unaltered. The frequency of CD8 alpha+ IEL expressing CD8 beta, in H-2Db male mice, however, is significantly decreased and these cells do not express the transgenic TCR. In contrast, virtually all CD8 alpha+beta- IEL in the same animals express the transgenic TCR. Still, these potentially autoreactive cells are refractile to H-Y/H-2Db stimulation in vitro. Both H-2Db and H-2Dd transgenic females contain high frequencies of cells expressing the transgenic TCR among CD8 alpha+beta- and CD8 alpha+beta+ IEL. However, two possibly related phenotypic features are peculiar to H-2Db female mice. The frequency of CD8 alpha+ IEL expressing CD8 beta is increased in these mice and, while in H-2Dd females the level of the transgenic TCR alpha chain expressed on CD8 alpha+beta+ IEL is uniformly low, some of the CD8 alpha+beta+ IEL in H-2Db females express a high level of both transgenic TCR chains. It is important to note, the ability of CD8 alpha+beta+ IEL to respond to H-Y/H-2Db stimulation in vitro is restricted to those coexpressing a high level of both transgenic TCR chains. The analysis of athymic radiation chimeras using adult thymectomized recipients of distinct H-Y/H-2 haplotypes, reconstituted with bone marrow from H-2Db transgenic females, demonstrates that all IEL subsets present in unmanipulated transgenic animals develop in the absence of a thymus. These IEL are phenotypically identical to those found in unmanipulated transgenic animals sharing the H-Y/H-2 haplotype of athymic recipients. Taken together, these results demonstrate that in the absence of male antigen, expression of H-2Db in the intestinal epithelium results in the positive selection of functional IEL specific for male antigen, in situ. When both H-Y and H-2Db are expressed in the intestinal epithelium, CD8 alpha+beta+ IEL expressing the transgenic TCR are negatively selected, while the frequency of nonfunctional CD8 alpha+beta- IEL expressing the transgenic CR is increased.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
KARIMA KARA ◽  
MALIKA RACHED-KANOUNI ◽  
SAMAH MNASRI ◽  
Hichem Khammar ◽  
M’BAREK BEN NACEUR
Keyword(s):  
Triticum Aestivum ◽  
Genetic Variability ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Genetic Similarity ◽  
Triticum Aestivum L ◽  
Breeding Programs ◽  
High Level ◽  
Derived Data ◽  
Selection Of

Abstract. Kara K, Rached-Kanouni M, Mnasri S, Khammar H, Ben Naceur MB. 2020. Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers. Biodiversitas 21: 2638-2644. The assessment of genetic diversity is a key step in evaluating the adaptation of populations to new environmental conditions, and thus in the selection of new cultivars. The present investigation aimed to assess genetic variability of bread wheat (Triticum aestivum L.) genotypes grown in Algeria. The 17 hexaploid genotypes of bread wheat were assessed using 16 molecular microsatellites of SSR (Simple Sequence Repeat). Among the 16 microsatellite markers tested, only 11 markers were the most polymorphic and reproducible. The Polymorphism Information Content (PIC) values per locus varied from 0.14 to 0.70 with an average of 0.48 and 0.49. Genetic similarity between genotypes varied from 0.27 and 0.92 with an average of 0.60. The highest genetic distance value of 0.92 has been scored between Kauz/Pastor/Fiscal and Wbll1*2/Brambling. The lowest value of 0.27 has been scored between Cham6 and Pastor/Wbll1. Genetic similarity was calculated by molecular derived data and used to produce a dendrogram. The genotypes were grouped in two clear clusters according to their origin and pedigree. The first cluster included wheat genotypes Ain Abid (local genotypes), Attila/2 Pastor, and 5119 (introduced genotypes). The second group revealed high polymorphism and was subdivided into four sub-groups. This high level of diversity revealed among the accessions of wheat, grown in Algeria could be used in breeding programs.

scholarly journals The SOFG Anatomy Entry List (SAEL): An Annotation Tool for Functional Genomics Data

2004 ◽  
Vol 5 (6-7) ◽  
pp. 521-527 ◽  
Author(s):  
Helen Parkinson ◽  
Stuart Aitken ◽  
Richard A. Baldock ◽  
Jonathan B. L. Bard ◽  
Albert Burger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Functional Genomics ◽  
Large Scale ◽  
Controlled Vocabulary ◽  
First Pass ◽  
Relevant Term ◽  
High Level ◽  
Supporting Functional ◽  
Selection Of

A great deal of data in functional genomics studies needs to be annotated with low-resolution anatomical terms. For example, gene expression assays based on manually dissected samples (microarray, SAGE, etc.) need high-level anatomical terms to describe sample origin. First-pass annotation in high-throughput assays (e.g. large-scalein situgene expression screens or phenotype screens) and bibliographic applications, such as selection of keywords, would also benefit from a minimum set of standard anatomical terms. Although only simple terms are required, the researcher faces serious practical problems of inconsistency and confusion, given the different aims and the range of complexity of existing anatomy ontologies. A Standards and Ontologies for Functional Genomics (SOFG) group therefore initiated discussions between several of the major anatomical ontologies for higher vertebrates. As we report here, one result of these discussions is a simple, accessible, controlled vocabulary of gross anatomical terms, the SOFG Anatomy Entry List (SAEL). The SAEL is available from http://www.sofg.org and is intended as a resource for biologists, curators, bioinformaticians and developers of software supporting functional genomics. It can be used directly for annotation in the contexts described above. Importantly, each term is linked to the corresponding term in each of the major anatomy ontologies. Where the simple list does not provide enough detail or sophistication, therefore, the researcher can use the SAEL to choose the appropriate ontology and move directly to the relevant term as an entry point. The SAEL links will also be used to support computational access to the respective ontologies.

scholarly journals V.—What is Laterite?

1911 ◽  
Vol 8 (11) ◽  
pp. 507-516 ◽  
Author(s):  
L. Leigh Fermor
Keyword(s):  
Great Variability ◽  
Low Level ◽  
Upper Limit ◽  
Great Utility ◽  
The Individual ◽  
High Level

Detrital Laterite or Lateritite.—Many of the so-called low-level laterites and some of the high-level laterites consist of admixtures of detritus from the various varieties of laterite already mentioned, with quartz and argillaceous detritus from granites and gneisses, the whole cemented together by the action of water on the lateritic constituents of the mixture. For such rocks the name detrital laterite is available, and could be used in all cases in which the reconstructed rock contained, say, at least 40 per cent of lateritic constituents. Theoretically, detrital laterites may contain, as an upper limit, 100 per cent of lateritic constituents, corresponding to the case of a detrital rock composed entirely of fragments of a pure laterite, without admixture of extraneous constituents. From the above it will be seen that I would allow greater elasticity in using the term laterite for the detrital forms than for those formed in situ (see next paragraph), provided the adjective detrital be carefully prefixed; for this word seems to me to imply the great variability of composition that must characterize these secondary laterites. But if it were considered desirable to avoid the use of the word laterite altogether in this connexion, the obvious term to use instead would seem to be lateritite, implying a rock the individual constituents of which are pieces of laterite. This term would be of great utility, and could be applied to all detrital and reconstructed lateritic rocks. The word is put forward here merely as a suggestion, but if geologists were to distinguish, in writing, between laterite, lateritoid, and lateritite, great clarity could be introduced into the literature of these substances.

Molecular cytogenetic characterization ofAegilops biuncialisand its use for the identification of 5 derived wheat – Aegilops biuncialisdisomic addition lines

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 1070-1082 ◽  
Author(s):  
Annamária Schneider ◽  
Gabriella Linc ◽  
István Molnár ◽  
Márta Molnár-Láng
Keyword(s):  
Triticum Aestivum ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Addition Lines ◽  
Aegilops Umbellulata ◽  
Aegilops Comosa ◽  
Disomic Addition Lines ◽  
M Genome

The aim of the experiments was to produce and identify different Triticum aestivum – Aegilops biuncialis disomic addition lines. To facilitate the exact identification of the Ae. biuncialis chromosomes in these Triticum aestivum – Ae. biuncialis disomic additions, it was necessary to analyze the fluorescence in situ hybridization (FISH) pattern of Ae. biuncialis (2n = 4x = 28, UbUbMbMb), comparing it with the diploid progenitors (Aegilops umbellulata, 2n = 2x = 14, UU and Aegilops comosa, 2n = 2x = 14, MM). To identify the Ae. biuncialis chromosomes, FISH was carried out using 2 DNA clones (pSc119.2 and pAs1) on Ae. biuncialis and its 2 diploid progenitor species. Differences in the hybridization patterns of all chromosomes were observed among the 4 Ae. umbellulata accessions, the 4 Ae. comosa accessions, and the 3 Ae. biuncialis accessions analyzed. The hybridization pattern of the M genome was more variable than that of the U genome. Five different wheat – Ae. biuncialis addition lines were produced from the wheat – Ae. biuncialis amphiploids produced earlier in Martonvásár. The 2M, 3M, 7M, 3U, and 5U chromosome pairs were identified with FISH using 3 repetitive DNA clones (pSc119.2, pAs1, and pTa71) in the disomic chromosome additions produced. Genomic in situ hybridization (GISH) was used to differentiate the Ae. biuncialis chromosomes from wheat, but no chromosome rearrangements between wheat and Ae. biuncialis were detected in the addition lines.Key words: Triticum aestivum, Aegilops biuncialis, fluorescence in situ hybridization, genomic in situ hybridization, wheat – Aegilops biuncialis addition lines.

scholarly journals Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis

2009 ◽  
Vol 57 (2) ◽  
pp. 69-78
Author(s):  
Hana Nevimová ◽  
Jan Bednář ◽  
Tomáš Vyhnánek
Keyword(s):  
Microsatellite Markers ◽  
Ssr Markers ◽  
Agricultural Research ◽  
Diversity Index ◽  
Rhynchosporium Secalis ◽  
Agricultural Research Institute ◽  
The Individual ◽  
Statistical Indicators ◽  
Barley Genotypes ◽  
Selection Of

The objective of the present study was to explore the polymorphism of microsatellite markers localised on chromosomes 3H and 7H in 15 genotypes of barley (Hordeum vulgareL.), spring form (2n = 2x = 14 chromosomes, genome HVHV) from the collection of genetic resources of the Agricultural Research Institute Kroměříž, Ltd. showing various degrees of susceptibility toRhynchosporium secalis. The selection of SSR markers was based on hitherto achieved knowledge according to which the greatest amount of resistance genes againstRhynchosporium secalisis localised on chromosomes 3H and 7H of barley. We selected 33 SSR markers for the analyses; 17 were localised on chromosome 3H of barley and 16 on chromosome 7H. Out of the total 33 SSR markers, 32 were polymorphous and one mar­ker (Bmac0282) was monomorphic. In total we detected 172 alleles ranging between 101 and 235 bp; the average number of alleles per locus was 5.21. In terms of the polymorphism of the SSR markers localised on chromosomes 3H and 7H the highest polymorphism (60%) was detected in theBmag0006andBmag0021SSR markers; the lowest in theBmag0877andEBmac0713markers, i.e. 20% and 13.3%, respectively. The average polymorphism based on analyses of 17 SSR markers on chromosome 3H was 37.6% and of 16 SSR markers on chromosome 7H was 31.3%. We also calculated the statistical indicators of the variability rate characteristics of the individual microsatellite markers: diversity index (DI) which ranged between 0.000 and 0.907 (on average 0.704); polymorphous information content (PIC) ranging between 0.000 and 0.906 (on average 0.679); and probability identity (PI) ranging between 0.006 and 1.000 (on average 0.137). On the basis of constructed dendrograms for SSR markers of both chromosomes together it was possible to divide the analysed set into cluster I of genotypes resistant and cluster II of genotypes susceptible and moderately susceptible toRhynchosporium secalis, and was not possible in dendrograms of individual chromosomes.

Pengaruh Persepsi Kegunaan, Persepsi Kemudahan, Kecepatan, Keamanan 75 dan Kerahasiaan Serta Kesiapan Teknologi Informasi Wajib Pajak terhadap Penggunaan e-Filing Bagi Wajib Pajak Orang Pribadi Di Kota Tangerang, Kecamatan Karawaci

2016 ◽  
Vol 8 (1) ◽  
pp. 75-91 ◽  
Author(s):  
Shelby Devina ◽  
Waluyo Waluyo
Keyword(s):  
Ease Of Use ◽  
Perceived Usefulness ◽  
Security And Privacy ◽  
Primary Data ◽  
Perceived Ease Of Use ◽  
Analysis Technique ◽  
Convenience Sampling ◽  
The Individual ◽  
Tax Payers ◽  
Selection Of

The objective of this research was to examine the effect of perceived usefulness, perceived ease of use, speed, security and privacy and readiness technology tax payers information to e-Filing usage. The object of this study is the individual tax payers in Tangerang City, Karawaci District. The selection of the sample is determined based on convenience sampling method. Data used in this study was primary data, id est: questionnaires. The respondent in this study were 110. Data analysis technique in this study using multiple linear regression. The result of this study were (1) perceived usefulness have a significant impact towards e-Filing usage; (2) perceived ease of use have a significant impact towards e-Filing usage; (3) speed does not have a significant impact towards e-Filing usage; (4) security and privacy does not have a significant impact towards e-Filing usage; (5) readiness technology tax payers information does not have a significant impact towards e-Filing usage; (6) perceived usefulness, perceived ease of use, speed, security and privacy and readiness technology tax payers information all simultaneously, have a significant impact towards e-Filing usage. Keywords: e-Filing usage, perceived usefulness, perceived ease of use, readiness technology tax payers information, security and privacy.

Territorial Distribution of Alcohol and Drug Addictions Mortality Concerning Regional Disparities in the Slovak Republic from Year 1996 to Year 2015

2020 ◽  
Keyword(s):  
Mortality Rate ◽  
Slovak Republic ◽  
Regional Disparities ◽  
Point Of View ◽  
Extreme Position ◽  
Data Set ◽  
Female Sex ◽  
Male Sex ◽  
The Individual ◽  
High Level

BACKGROUND: This paper deals with territorial distribution of the alcohol and drug addictions mortality at a level of the districts of the Slovak Republic. AIM: The aim of the paper is to explore the relations within the administrative territorial division of the Slovak Republic, that is, between the individual districts and hence, to reveal possibly hidden relation in alcohol and drug mortality. METHODS: The analysis is divided and executed into the two fragments – one belongs to the female sex, the other one belongs to the male sex. The standardised mortality rate is computed according to a sequence of the mathematical relations. The Euclidean distance is employed to compute the similarity within each pair of a whole data set. The cluster analysis examines is performed. The clusters are created by means of the mutual distances of the districts. The data is collected from the database of the Statistical Office of the Slovak Republic for all the districts of the Slovak Republic. The covered time span begins in the year 1996 and ends in the year 2015. RESULTS: The most substantial point is that the Slovak Republic possesses the regional disparities in a field of mortality expressed by the standardised mortality rate computed particularly for the diagnoses assigned to the alcohol and drug addictions at a considerably high level. However, the female sex and the male sex have the different outcome. The Bratislava III District keeps absolutely the most extreme position. It forms an own cluster for the both sexes too. The Topoľčany District bears a similar extreme position from a point of view of the male sex. All the Bratislava districts keep their mutual notable dissimilarity. Contrariwise, evaluation of a development of the regional disparities among the districts looks like notably heterogeneously. CONCLUSIONS: There are considerable regional discrepancies throughout the districts of the Slovak Republic. Hence, it is necessary to create a common platform how to proceed with the solution of this issue.

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