Glass Bone Implants: The Effect of Surface Topology on Attachment and Proliferation of Osteoblast Cells on 45S Bioactive Glass

2009 ◽  
Vol 1235 ◽  
Author(s):  
Raina Himani Jain ◽  
Shaojie Wang ◽  
Hassan M. Moawad ◽  
Matthias M. Falk ◽  
Himanshu Jain
Keyword(s):  
Bioactive Glass ◽  
Bone Cells ◽  
Cell Attachment ◽  
Surface Characteristics ◽  
Past Research ◽  
Surface Topology ◽  
Bone Implants ◽  
Adhesion Sites ◽  
Effect Of Surface

AbstractBioglass 45S is a promising bone implant material with superior biocompatibility. Past research showed that adhesion of bone cells to titanium is strongly affected by its surface architecture. However, little is known about the role of surface topology of glass on its use as an implant. Thus, we systematically investigated the effect of surface roughness (Ra ∼ 0.01 – 1.2 μm) on cell adhesion and proliferation on 45S Bioglass in vitro. MG63 osteosarcoma and MC3T3 osteoblast precursor cells were seeded on the glass samples, and incubated for up to 6 days. The attachment, morphology and proliferation of cells were investigated using fluorescence microscopy. Our results show that cell attachment (as indicated by cell spreading and number of focal adhesion sites), and proliferation rate decrease with increasing roughness of bioactive glass surface. These findings provide important insight for improving surface characteristics of bioactive glass bone implants.

scholarly journals Development of 3D Bioactive Nanocomposite Scaffolds Made from Gelatin and Nano Bioactive Glass for Biomedical Applications

2010 ◽  
Vol 19 (2) ◽  
pp. 096369351001900 ◽  
Author(s):  
M. Mozafari ◽  
F. Moztarzadeh ◽  
M. Rabiee ◽  
M. Azami ◽  
N. Nezafati ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Biomedical Applications ◽  
Cell Attachment ◽  
Tissue Engineering Scaffolds ◽  
Study Results ◽  
Nanocomposite Scaffold ◽  
Nanocomposite Scaffolds ◽  
First Time

In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fabricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting combined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 μm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaffolds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.

scholarly journals The Anchorage of Bone Cells onto an Yttria-Stabilized Zirconia Surface with Mild Nano-Micro Curved Profiles

2020 ◽  
Vol 8 (4) ◽  
pp. 127
Author(s):  
Susanne Staehlke ◽  
Armin Springer ◽  
Thomas Freitag ◽  
Jakob Brief ◽  
J. Barbara Nebe
Keyword(s):  
Matrix Protein ◽  
Bone Cells ◽  
Cell Attachment ◽  
Yttria Stabilized Zirconia ◽  
Extracellular Matrix Protein ◽  
Cell Phenotype ◽  
Ros Generation ◽  
Stabilized Zirconia ◽  
Dental Surgery

The high biocompatibility, good mechanical properties, and perfect esthetics of ceramic dental materials motivate investigation into their suitability as an endosseous implant. Osseointegration at the interface between bone and implant surface, which is a criterion for dental implant success, is dependent on surface chemistry and topography. We found out earlier that osteoblasts on sharp-edged micro-topographies revealed an impaired cell phenotype and function and the cells attempted to phagocytize these spiky elevations in vitro. Therefore, micro-structured implants used in dental surgery should avoid any spiky topography on their surface. The sandblasted, acid-etched, and heat-treated yttria-stabilized zirconia (cer.face®14) surface was characterized by scanning electron microscopy and energy dispersive X-ray. In vitro studies with human MG-63 osteoblasts focused on cell attachment and intracellular stress level. The cer.face 14 surface featured a landscape with nano-micro hills that was most sinusoidal-shaped. The mildly curved profile proved to be a suitable material for cell anchorage. MG-63 cells on cer.face 14 showed a very low reactive oxygen species (ROS) generation similar to that on the extracellular matrix protein collagen I (Col). Intracellular adenosine triphosphate (ATP) levels were comparable to Col. Ceramic cer.face 14, with its sinusoidal-shaped surface structure, facilitates cell anchorage and prevents cell stress.

In Vitro Human Osteoblast Responses to Titanium Oxide-Based Surfaces with Varying Topology and Composition

2009 ◽  
Vol 1187 ◽  
Author(s):  
Charles Andrew Collier ◽  
Julien M. Paillard ◽  
Athina E. Markaki ◽  
James A. Curran ◽  
Helen J Griffiths ◽  
...  
Keyword(s):  
Titanium Oxide ◽  
Cellular Proliferation ◽  
Surface Characteristics ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Surface Topology ◽  
Implant Design ◽  
Cell Responses ◽  
Alp Activity

AbstractThe surface topology and composition of prosthetic implant materials affect cell responses and are therefore important design features. Plasma electrolytic oxidation (PEO) is a surface modification technique that can be used to produce oxidized surfaces with various surface properties. In this work, Ti-6Al-4V was PEO processed to give two surfaces with different morphologies but similar chemical composition. Surface characteristics were assessed using X ray diffraction, scanning electron microscopy, energy dispersive spectroscopy, stylus profilometry and contact angle measurement.In vitro culture of human foetal osteoblasts (HOB) was performed on the surfaces, to examine cell responses to them. Cellular proliferation, morphology and differentiation were examined, using the AlamarBlue assay, SEM imaging and an alkaline phosphatase (ALP) activity assay respectively. Additionally, the individual effects of oxides present in the PEO processed surfaces (rutile and anatase) on the cells were examined, by binding them in powder form to produce surfaces with similar morphology, but different composition.Changes in the topology and chemistry of the surfaces affected osteoblast response. HOB proliferated more on the rougher PEO surface, and also displayed greater ALP activity. Also, cells responded differently to surfaces containing just rutile or anatase, indicating that the chemical phase of titanium oxide is of consequence for implant design.

Defective stimulation of proliferation and collagen biosynthesis of human bone cells by serum from diabetic patients

1992 ◽  
Vol 127 (6) ◽  
pp. 509-514 ◽  
Author(s):  
Rolf E Brenner ◽  
Bert Riemenschneider ◽  
Werner Blum ◽  
Martin Mörike ◽  
Walter M Teller ◽  
...  
Keyword(s):  
Metabolic Control ◽  
Collagen Synthesis ◽  
Bone Cells ◽  
Cell Attachment ◽  
Cell Number ◽  
Human Bone ◽  
Diabetic Patients ◽  
Inhibitory Influence ◽  
Control Serum

We studied the influence of fasting serum from nine insulin-dependent diabetic children and adolescents under insufficient metabolic control on normal human bone cells in vitro compared with serum from eight sex- and age-matched controls. Cell number 24 h after plating was significantly less under diabetic serum, indicating impaired cell attachment, spreading and initiation of cell proliferation. Cell number after five days was reduced by 1% diabetic serum, while higher serum concentrations had diverging effects on osteoblast proliferation. Collagen synthesis of human osteoblasts was significantly reduced by 8% diabetic serum compared to 8% control serum, while synthesis of non-collagenous proteins was not affected. Duration of diabetes (several weeks up to 12 years) had no influence on these parameters. The serum from one patient, which was studied a second time under excellent metabolic control three months later, however, had lost its inhibitory influence on collagen synthesis of osteoblasts. The pattern of the interstitial collagen types I, III and V was not altered by diabetic serum. These results indicate that defective regulation of proliferation and collagen synthesis of osteoblasts by components present in human diabetic serum may be an important factor in the development of diabetic osteopenia. The negative influence might be explained in part by reduced levels of IGF-I and elevated levels of IGF binding protein-1 in the diabetic sera.

Biological evaluation of micro-patterned hyaluronic acid hydrogel for bone tissue engineering

2014 ◽  
Vol 86 (12) ◽  
pp. 1911-1922 ◽  
Author(s):  
Hyo Seung Park ◽  
Su Yeon Lee ◽  
Hyunsik Yoon ◽  
Insup Noh
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Cells ◽  
Cell Attachment ◽  
Biological Properties ◽  
Biological Evaluation ◽  
Micro Patterning

Abstract Design of micro-patterning of hydrogel is of critical importance in both understanding cellular behaviors and mimicking controlled microenvironments and architectures of diverse well-organized tissues. After micro-patterning of hyaluronic acid (HA) hydrogel on a poly(dimethyl siloxane) substrate, its physical and biological properties have been compared with those of a non-patterned hydrogel for its possible applications in bone tissue engineering. The micro-patterned morphologies of HA hydrogel in both swollen and dehydrated forms have been observed with light microscope and scanning electron microscope, respectively, before and after in vitro cell culture. When MC3T3 bone cells were in vitro cultured on both HA hydrogels, the micro-patterned one shows excellence in cell proliferation and lining for 7 days along the micro-pattern paths over those of the non-patterned one, which have shown less cell-adhesiveness. The cytotoxicity of the micro-patterned HA hydrogels was in vitro evaluated by the assays of MTT, BrdU and Neutral red. The viability and morphology of MC3T3 cells on both HA hydrogels were observed with a fluorescence microscope by the live & dead assay, where their viability was confirmed by staining of F-actin development. The results of their H&E staining showed that both micro-patterned and non-patterned hydrogels induced development of tissue regeneration as observed by cell attachment, proliferation, and survivability, but the micro-patterned one induced distinctive patterning of both better initial cells adhesion on the micro-patterns and subsequently development of their proliferation and extracellular matrix, which were considered as important characteristics in their applications to tissue engineering.

Study on the Cytotoxicity In Vitro of Composite Materials Based on Poly(L-Lactide-Co-Glycolide) and Bioactive Glass

2011 ◽  
Vol 685 ◽  
pp. 384-389 ◽  
Author(s):  
Zhi Hua Zhou ◽  
Yong Yi Gao ◽  
Qing Feng Yi ◽  
Qing Quan Liu ◽  
Li Hua Liu
Keyword(s):  
Bioactive Glass ◽  
Cell Attachment ◽  
Biological Response ◽  
Normal Growth ◽  
Cytotoxicity Test ◽  
Medical Field ◽  
Proliferation And Differentiation ◽  
Fibroblastic Cells ◽  
Cells Cultured

The effect of poly(L-lactide-co-glycolide)/bioactive glass (PLGA/BG) on cell attachment, proliferation and differentiation of L929 fibroblastic cells was investigated. The results ofin-vitrocytotoxicity test indicated that the cells cultured in extract of PLGA/BG and on the surface of composite showed normal growth and proliferation. The cell proliferation and alkaline phosphatase (ALP) activity of fibroblast were significantly improved after 3 and 7 days of culture on PLGA/BG films in comparison with PLGA films. It can be concluded that the addition of bioactive glass into PLGA stimulates the proliferation and differentiation of fibroblastic cells. Therefore, PLGA/BG composites have a promising biological response as a potential biomaterial in medical field.

In vitro Biocompatibility Tests of Glass Ionomer Cements Impregnated with Collagen or Bioactive glass to Fibroblasts

2012 ◽  
Vol 36 (3) ◽  
pp. 269-274 ◽  
Author(s):  
C Subbarao ◽  
P Neelakantan ◽  
CV Subbarao
Keyword(s):  
Bioactive Glass ◽  
Cell Attachment ◽  
Glass Ionomer Cement ◽  
Mineral Trioxide Aggregate ◽  
Fibroblast Cell ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Electron Microscopes ◽  
Ionomer Cement

Aim and Design: To evaluate the biocompatibility of glass ionomer cement (GIC) impregnated with collagen or bioactive glass to BHK-21 fibroblasts in vitro. Mineral Trioxide Aggregate was used as the standard for comparison. Human maxillary central incisors (n=70) were instrumented with a rotary NiTi system and filled. Following resection of the apical 3mm, root end cavities were prepared and restored with conventional GIC (group 1) or GIC with 0.01%, 0.1% or 1% collagen (groups 2, 3, 4 respectively) or, 10%, 30 % or 50% bioactive glass (groups 5,6,7 respectively), or Mineral Trioxide Aggregate (group 8). The root slices were incubated in tissue culture plates with BHK-21 fibroblast cell line. Phase contrast and scanning electron microscopes were used to score cell quantity, morphology and cell attachment. The data were statistically analyzed by one way ANOVA with Post Hoc Tukey HSD test (p = 0.05). Results and conclusions: Group 5 showed the highest scores which was significantly higher than all other groups (p<0.05) except group 8, with which there was no significant difference (p>0.05). Glass ionomer cement with 10% bioactive glass showed better adhesion and spreading of cells than glass ionomer cement with 0.01% collagen. The biocompatibility of collagen and bioactive glass was concentration dependent. The addition of bio active glass improved the biocompatibility of glass ionomer cement to fibroblasts better than addition of collagen.

Synergistic effects of elastic modulus and surface topology of Ti-based implants on early osseointegration

RSC Advances ◽  
2016 ◽  
Vol 6 (49) ◽  
pp. 43685-43696 ◽  
Author(s):  
Xiaohan Dai ◽  
Xuehui Zhang ◽  
Mingming Xu ◽  
Ying Huang ◽  
Boon Chin Heng ◽  
...  
Keyword(s):  
Elastic Modulus ◽  
Synergistic Effect ◽  
Osteogenic Differentiation ◽  
Cell Attachment ◽  
Synergistic Effects ◽  
Surface Topology ◽  
Ti Alloy ◽  
Micro Scale

Elastic modulus and surface micro-scale topographical structure of Ti alloy implants have a synergistic effect on cell attachment, osteogenic differentiation of rBMSCs in vitro and early osseointegration in vivo.

scholarly journals Evidence of ectokinase-mediated phosphorylation of osteopontin and bone sialoprotein by osteoblasts during bone formation in vitro

1997 ◽  
Vol 323 (3) ◽  
pp. 637-643 ◽  
Author(s):  
XinLi ZHU ◽  
Chun LUO ◽  
Jack M. FERRIER ◽  
Jaro SODEK
Keyword(s):  
Cell Surface ◽  
Bone Cells ◽  
Cell Attachment ◽  
Biological Properties ◽  
Bone Cell ◽  
Bone Sialoprotein ◽  
Surface Proteins ◽  
Pulse Labelling ◽  
Mediate Cell

Osteopontin (OPN) and bone sialoprotein (BSP) are phosphorylated glycoproteins that, together with osteonectin/secreted protein, acidic, rich in cysteine (SPARC) and osteocalcin, comprise the major non-collagen proteins of bone. Although phosphorylation of OPN and BSP, which is known to influence the biological properties of these proteins, has been shown to occur intracellularly, recent studies have demonstrated ectokinase activity in bone cell populations [Mikuni-Takagaki, Kakai, Satoyoshi, Kawano, Suzuki, Kawase and Saito (1995) J. Bone Miner. Res. 10, 231–241]. To determine whether OPN and BSP are phosphorylated by ectokinase activity we have used [γ-32P]ATP and [γ-32P]GTP as cell-impenetrable phosphate donors to analyse for ectokinase activity in osteoblastic UMR106.06 cells and fetal rat calvarial cells (FRCCs). By pulse-labelling confluent cells with radiolabelled nucleotides, the phosphorylation of endogenous and exogenously added OPN and BSP was demonstrated together with the labelling of a number of cell surface proteins. These phosphorylation reactions were inhibited by a cell-impermeable ectokinase inhibitor, K252b, and cell surface phosphorylation was also inhibited by exogenously added OPN and BSP substrates, indicating competition for the ectokinase enzyme. However, phosphorylation of OPN and BSP, both of which can mediate cell attachment through Arg-Gly-Asp (RGD) motifs, was not inhibited by an RGD peptide, suggesting that binding of OPN and BSP to cell surface integrins is not required. In similar experiments, ectokinase-mediated phosphorylation of OPN and BSP was demonstrated during mineralized tissue formation by FRCCs in vitro. These studies demonstrate that OPN and BSP secreted by bone cells are phosphorylated by a casein kinase II-like ectokinase present on the surface of osteoblastic cells.

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