A Protein Patterning Technique And Its Application In Bio-Inspired Self-Assembly

2002 ◽  
Vol 761 ◽  
Author(s):  
D. Guo ◽  
H. McNally ◽  
M. Pingle ◽  
D. Bergstrom ◽  
R. Bashir
Keyword(s):  
Self Assembly ◽  
Specific Binding ◽  
Gold Atom ◽  
Protein Patterning ◽  
Contact Mode ◽  
Microelectronic Fabrication ◽  
Micron Size ◽  
Patterning Technique ◽  
Lift Off ◽  
20 Nm

ABSTRACTProtein patterning techniques are crucial for the development of antibody-based biosensor and the study of controlled cell growth. This paper discusses a protein patterning technique based on microelectronic fabrication, DNA hybridization and biotin-streptavidin pair. A gold-on-silicon-dioxide substrate with micron size pattern was fabricated with photolithography and lift-off process. The average surface roughness of the gold pattern is 4.3 nm, measured by contact mode AFM. Thiol derivatized single stranded DNA was attached to the gold pattern surface by the chemical bonding between gold atom and sulfur atom. Surface attached DNA was then hybridized with a biotin conjugated complementary DNA sequence. Thus, the gold pattern was translated into a biotin pattern with similar resolution. Fluorescein conjugated streptavidin was patterned as demonstration. Fluorescence microscopy shows relative uniform streptavidin coverage of micron resolution and low background non-specific binding. The proposed protein patterning technique takes advantage of the high resolution of modern microelectronic fabrication. It has the potential of reaching sub-micron resolution. The biotin-streptavidin pair provides extremely specific and stable linking for protein immobilization. To show its application in biological inspired self-assembly, this technique was used successfully in the self-assembly of 20 nm streptavidin conjugated gold particles.

A Protein Patterning Technique And Its Application In Bio-Inspired Self-Assembly

2002 ◽  
Vol 735 ◽  
Author(s):  
D. Guo ◽  
H. McNally ◽  
M. Pingle ◽  
D. Bergstrom ◽  
R. Bashir
Keyword(s):  
Self Assembly ◽  
Specific Binding ◽  
Gold Atom ◽  
Protein Patterning ◽  
Contact Mode ◽  
Microelectronic Fabrication ◽  
Micron Size ◽  
Patterning Technique ◽  
Lift Off ◽  
20 Nm

ABSTRACTProtein patterning techniques are crucial for the development of antibody-based biosensor and the study of controlled cell growth. This paper discusses a protein patterning technique based on microelectronic fabrication, DNA hybridization and biotin-streptavidin pair. A gold-on-silicon-dioxide substrate with micron size pattern was fabricated with photolithography and lift-off process. The average surface roughness of the gold pattern is 4.3 nm, measured by contact mode AFM. Thiol derivatized single stranded DNA was attached to the gold pattern surface by the chemical bonding between gold atom and sulfur atom. Surface attached DNA was then hybridized with a biotin conjugated complementary DNA sequence. Thus, the gold pattern was translated into a biotin pattern with similar resolution. Fluorescein conjugated streptavidin was patterned as demonstration. Fluorescence microscopy shows relative uniform streptavidin coverage of micron resolution and low background non-specific binding. The proposed protein patterning technique takes advantage of the high resolution of modern microelectronic fabrication. It has the potential of reaching sub-micron resolution. The biotin-streptavidin pair provides extremely specific and stable linking for protein immobilization. To show its application in biological inspired self-assembly, this technique was used successfully in the self-assembly of 20 nm streptavidin conjugated gold particles.

Bio-inspired Self-Assembly of Micro and Nano-Structures for Sensing and Electronic Applications

2002 ◽  
Vol 739 ◽  
Author(s):  
H. McNally ◽  
S. W. Lee ◽  
D. Guo ◽  
M. Pingle ◽  
D. Bergstrom ◽  
...  
Keyword(s):  
Electric Fields ◽  
Self Assembly ◽  
Critical Role ◽  
Protein Patterning ◽  
Micro Scale ◽  
Microelectronic Fabrication ◽  
Electro Optic ◽  
Novel Material ◽  
Hybrid Devices ◽  
20 Nm

ABSTRACTBio-inspired assembly, through the use of bio-molecules such as DNA and proteins, will play a critical role in the advancement of novel sensing techniques and for the realization of heterogeneous integration of materials. For many of these applications, such as antibody-based biosensor and the study of controlled cell growth, DNA and protein patterning techniques are crucial. We will present an update of our work on protein patterning techniques using microelectronic fabrication, DNA hybridization and biotin-streptavidin pairing. To show its application in biological inspired self-assembly, this technique was used successfully in the self-assembly of 20 nm streptavidin conjugated gold particles. In addition, the integration of nano-and micro-scale heterogeneous materials is very important for novel material synthesis and electro-optic applications. We will present an update on our work to assemble silicon electronic devices using DNA/charged molecules and electric fields. Devices are fabricated, released, charged with molecules, and subsequently manipulated in electric fields. The techniques described can be used to integrate the hybrid devices such as nano- or micro-scale resistors, PN diodes, and MOSFETs on silicon or other substrates such as glass, plastic, etc.

Nanostructured Polymetallic Powders to Create New Functional Materials on its Base

2015 ◽  
Vol 670 ◽  
pp. 49-54 ◽  
Author(s):  
Yuriy A. Zaharov ◽  
Valeriy M. Pugachev ◽  
Kseniya A. Datiy ◽  
Anna N. Popova ◽  
Anastasiya S. Valnyukova ◽  
...  
Keyword(s):  
Particle Size ◽  
Phase Diagrams ◽  
Spatial Organization ◽  
Functional Materials ◽  
Particle Morphology ◽  
Cloud Type ◽  
Synthesis Conditions ◽  
Micron Size ◽  
Common Nature ◽  
20 Nm

In the paper, the particle morphology is considered and the slices of phase diagrams of nanosystems agreeable to the synthesis conditions are constructed according to the data obtained earlier by authors, as well as new results of the study of nanostructured Fe-Co, Fe-Ni, Co-Ni, Fe-Co-Ni, Fe-Pt, Cu-Ni and Ni-Cd powders. It is found that all considered polymetallic systems have common nature of the particle size spatial organization, i.e., 7-20 nm nanocrystals (for different systems) form highly compact aggregates (40-100 nm) which put together into loose porous agglomerates (up to 200-250 nm) and then into unconsolidated micron size formation of cloud type. It is classified uncovered features of nanostructured polymetallic phase diagrams in comparison with phase diagrams of bulk systems. Magnetic properties of nanosystems are studied.

scholarly journals In Vitro Assembly of the T=13 Procapsid of Bacteriophage T5 with Its Scaffolding Domain

2010 ◽  
Vol 84 (18) ◽  
pp. 9350-9358 ◽  
Author(s):  
Alexis Huet ◽  
James F. Conway ◽  
Lucienne Letellier ◽  
Pascale Boulanger
Keyword(s):  
Self Assembly ◽  
Scaffolding Protein ◽  
Accessory Proteins ◽  
Conformational Diversity ◽  
In Vitro Assembly ◽  
20 Nm ◽  
Head Protein ◽  
Bacteriophage T5 ◽  
Icosahedral Capsid

ABSTRACT The Siphoviridae coliphage T5 differs from other members of this family by the size of its genome (121 kbp) and by its large icosahedral capsid (90 nm), which is organized with T=13 geometry. T5 does not encode a separate scaffolding protein, but its head protein, pb8, contains a 159-residue aminoterminal scaffolding domain (Δ domain) that is the mature capsid. We have deciphered the early events of T5 shell assembly starting from purified pb8 with its Δ domain (pb8p). The self assembly of pb8p is regulated by salt conditions and leads to structures with distinct morphologies. Expanded tubes are formed in the presence of NaCl, whereas Ca2+ promotes the association of pb8p into contracted tubes and procapsids. Procapsids display an angular organization and 20-nm-long internal radial structures identified as the Δ domain. The T5 head maturation protease pb11 specifically cleaves the Δ domain of contracted and expanded tubes. Ca2+ is not required for proteolytic activity but for the organization of the Δ domain. Taken together, these data indicate that pb8p carries all of the information in its primary sequence to assemble in vitro without the requirement of the portal and accessory proteins. Furthermore, Ca2+ plays a key role in introducing the conformational diversity that permits the formation of a stable procapsid. Phage T5 is the first example of a viral capsid consisting of quasi-equivalent hexamers and pentamers whose assembly can be carried out in vitro, starting from the major head protein with its scaffolding domain, and whose endpoint is an icosahedral T=13 particle.

scholarly journals Extraction of Magnetite from Millscales Waste for Ultrafast Removal of Cadmium Ions

2019 ◽  
Vol 9 (1) ◽  
pp. 5902-5907
Keyword(s):  
Saturation Magnetization ◽  
Milling Time ◽  
Cost Effective ◽  
Xrd Analysis ◽  
High Energy ◽  
Cadmium Ions ◽  
Surface Areas ◽  
Magnetite Fe3o4 ◽  
Micron Size ◽  
20 Nm

This research was conducted to produce the magnetite (Fe3O4 ) nanoparticles extracted from the industrial millscale waste. Then, the micron size samples were extracted and grounded on the high energy ball milling (HEBM) at various milling time for 4, 8, 12, 16 and 20 h. The formation of nanosized single-phase hexagonal spinel has been observed with XRD analysis as early as 4 h milling time. The FTIR transmission spectrum shows the appearance of a Fe-O functional group for each sample. HRTEM images showed that all the samples had a small particle size of 5-20 nm with uniform distribution. The specific surface area of the 5 adsorbents increased after the 8 h milling time and it showed reduction after that. The magnetite adsorbents then utilized the adsorbent in Cadmium ions removal of the aqueous solution. Fe3O4 with 8 h milling time was able to remove 9.81 mg of cadmium ions with 1 g of adsorbents consume. The removal of the cadmium ions detected related to the particles size, surface areas and saturation magnetization. This research successfully revealed that the higher saturation magnetization contributed to high removal percentages in cadmium ions of aqueous solutions. Fe3O4 extraction from mill scales waste is cost-effective, the process is eco-friendly and thus, potentially to be applied for wastewater treatment.

scholarly journals Multivalency Pattern Recognition to Sort Colloidal Assemblies

2020 ◽  
Author(s):  
Sebastian Loescher ◽  
Andreas Walther
Keyword(s):  
Pattern Recognition ◽  
Self Assembly ◽  
Soft Matter ◽  
Biological Response ◽  
Interaction Patterns ◽  
Colloidal Systems ◽  
Binding Motifs ◽  
Important Principle ◽  
Additional Control ◽  
20 Nm

<i>Multivalent interactions are an important principle for self-assembly, and have been widely used to assemble colloidal systems. However, binding partners on colloids are typically statistically distributed, which falls short of the possibilities arising from geometrically controlled multivalency patterns as for instance found in viruses. Herein, we use the ultimate precision provided by 3D DNA origamis to introduce colloidal scale multivalency pattern recognition via designing geometrically precise interaction patterns at patches of patchy nanocylinder. This gives rise to self-sorting of colloidal assemblies despite having the same type and number of supramolecular binding motifs – solely based on the pattern located on a 20 x 20 nm cross section. The degree of sorting can be modulated by the geometric overlap of patterns and homo, mixed and alternating supracolloidal polymerizations are demonstrated. We demonstrate that geometric positioning of multivalency patterns provides additional control to organize soft matter, and we believe the concept to be of importance for engineering biological response and to be generalizable for other precision nanoparticles and soft matter objects.</i>

scholarly journals Extra Surfactant-Assisted Self-Assembly of Highly Ordered Monolayers of BaTiO3 Nanocubes at the Air—Water Interface

Nanomaterials ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 739 ◽  
Author(s):  
Hiroki Itasaka ◽  
Ken-Ichi Mimura ◽  
Kazumi Kato
Keyword(s):  
Self Assembly ◽  
Large Scale ◽  
Three Dimensional ◽  
Water Interface ◽  
Compression Pressure ◽  
Air Water Interface ◽  
Out Of Plane ◽  
Key Factor ◽  
Evaporation Induced Self Assembly ◽  
20 Nm

Assembly of nanocrystals into ordered two- or three-dimensional arrays is an essential technology to achieve their application in novel functional devices. Among a variety of assembly techniques, evaporation-induced self-assembly (EISA) is one of the prospective approaches because of its simplicity. Although EISA has shown its potential to form highly ordered nanocrystal arrays, the formation of uniform nanocrystal arrays over large areas remains a challenging subject. Here, we introduce a new EISA method and demonstrate the formation of large-scale highly ordered monolayers of barium titanate (BaTiO3, BT) nanocubes at the air-water interface. In our method, the addition of an extra surfactant to a water surface assists the EISA of BT nanocubes with a size of 15–20 nm into a highly ordered arrangement. We reveal that the compression pressure exerted by the extra surfactant on BT nanocubes during the solvent evaporation is a key factor in the self-assembly in our method. The BT nanocube monolayers transferred to substrates have sizes up to the millimeter scale and a high out-of-plane crystal orientation, containing almost no microcracks and voids.

Preparation and characterization of poly(styrenemethacrylic acid)/MCM-41 core/shell nanocomposite microspheres

e-Polymers ◽  
2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Yansheng Zhao ◽  
Xingji Ma ◽  
Yongmei Liu ◽  
Guangwei Yuan ◽  
Meijuan Guo ◽  
...  
Keyword(s):  
Self Assembly ◽  
Cetyltrimethylammonium Bromide ◽  
Average Diameter ◽  
Molar Ratio ◽  
Core Shell ◽  
Acidic Media ◽  
Assembly Method ◽  
20 Nm ◽  
Mcm 41

AbstractIn acidic media, poly(styrene-methacrylic acid)/MCM-41 [P(St- MAA)/MCM-41] core/shell microspheres were synthesized using monodisperse P(St-MAA) particles contained in soap-free emulsion and cetyltrimethylammonium bromide as co-templates by adsorption self-assembly method. The effects of P(St- MAA) composition on shell structure of the core/shell microspheres were investigated. The morphology and composition of P(St-MAA)/MCM-41 microspheres were characterized by TEM, XRD and FTIR. The results show that the ordering degree of MCM-41 shells increased as the molar ratio of MAA to St increased. When n(MAA)/n(St) is 0.2, the average diameter and the shell thickness of nanocomposite microspheres are about 170 nm and 20 nm, respectively.

The Specific Binding between Galactose Group and Ricinus Communis Agglutinin (RCA) Investigated with Microcantilever

2012 ◽  
Vol 531-532 ◽  
pp. 600-604
Author(s):  
Hui Yong Zhang ◽  
Ji Hu ◽  
Hui Min Liu
Keyword(s):  
Atomic Force Microscopy ◽  
Self Assembly ◽  
Control Experiment ◽  
Ricinus Communis ◽  
Specific Binding ◽  
Specific Interaction ◽  
Protein Layer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ricinus Communis Agglutinin

The specific recognization between galactose group and Ricinus Communis Agglutinin (RCA) was investigated by microcantilever. The gold side of the microcantilever was covalently bound with N-galactose, RCA and asialofetuin (ASF) via mixed self assembly monolayer of 11-mercaptoundecanoic acid and 6-mercaptohexanol, respectively. After adding RCA into the flowing cell, the deflection could be observed on the N-galactose or ASF modified microcantilever. Meanwhile, the deflection could also be observed after ASF bound to the RCA modified microcantilever. In order to prove that the deflection is caused by the specific interaction between the galactose group and RCA, bovine serum albumin (BSA) was introduced into the flowing cell as control experiment and no obvious deflection was observed. The specific interaction was also confirmed by the evidence that the bound protein layer can be mechanically removed with atomic force microscopy nanolithography technology.

Regenerated Antheraea pernyi Silk Fibroin Nanofiber Film

2012 ◽  
Vol 465 ◽  
pp. 160-164 ◽  
Author(s):  
Li Mao ◽  
Yu Liu ◽  
Xi Long Wu ◽  
Shen Zhou Lu
Keyword(s):  
Surface Tension ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Water Environment ◽  
Antheraea Pernyi ◽  
Thiocyanate Solution ◽  
Nanofiber Film ◽  
20 Nm ◽  
Cell Migration And Proliferation

Regenerated Antheraea Pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea Pernyi (A. pernyi) silk fiber in lithium thiocyanate solution. Atomic force microscopy (AFM) examination showed that there were many short nanofibers in regenerated ASF solution, which were made up of 10-20 nm nanospheres. In this paper, we discussed the formation mechanism of these nanofibers. The results of the surface tension showed that ASF had surface active and can significantly decline the surface tension of water from 73 mN/m to 51 mN/m. The hydrophobic side chains of ASF molecular would try to break away from water environment and thereby undergo self-assembly into nanospheres. And then, these nanospheres arrange into a short nanofiber. Then, the ASF nanofiber film was prepared by casting regenerated ASF solution blended with glycol. The structure of ASF was β-sheet and the film was water-insoluble. This blend film not only had excellent mechanical properties, but also can be biodegradated by enzyme in vitro. All of this indicated that this new regenerated ASF nanofiber film not only provided excellent biocompatibility, but also had large surface as extracellular matrix (ECM) to cell adhesion. The film made of nanofiber was similar to ECM on the nanoscale, which promoted cell migration and proliferation. In summary, it provided a great potential as a biological material.

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