scholarly journals Study of phospholipid molecular species of the green seaweed \(\textit{Halimeda incrassata}\) Lamx. from Truong Sa islands, Vietnam

2021 ◽  
Vol 21 (1) ◽  
pp. 77-84
Author(s):  
Hue Pham Thu ◽  
Tuyet Anh Nguyen Van ◽  
Long Pham Quoc ◽  
Thanh Le Tat
Keyword(s):  
Fatty Acids ◽  
Molecular Species ◽  
High Performance ◽  
Unsaturated Fatty Acids ◽  
High Resolution Mass Spectrometry ◽  
Saturated Fatty Acids ◽  
Phospholipid Molecular Species ◽  
Polar Lipid Class ◽  
Green Seaweed ◽  
Resolution Mass

In this report, by a high performance liquid chromatography (HPLC) - high resolution mass spectrometry (HRMS) method, 7 molecular species of phospholipid in the polar lipid class were identified including phosphatidylinositol (PI) and phosphatidylglycerol (PG). The PI 32:0 (16:0/16:0) and PG 34:3 (16:1/18:2) molecular species have the highest content. PI molecular species are mainly formed by saturated fatty acids (16:0, 18:0) and only one polyunsaturated fatty acids C20:4, while PG species are formed by unsaturated fatty acids (16:1, 18:2 and 18:3) and only one saturated fatty acid C16:0.

scholarly journals INVESTIGATION ON CONTENT OF FATTY ACIDS, PHOSPHOLIPIDS, AND PHOSPHOLIPID MOLECULAR SPECIES COMPOSITION OF THE VIETNAMESE CORAL Bebryce sp.

2018 ◽  
Vol 18 (2) ◽  
pp. 178-186
Author(s):  
Dang Thi Phuong Ly ◽  
Pham Minh Quan ◽  
Trinh Thi Thu Huong ◽  
Valeria P. Grigorchuk ◽  
Pham Quoc Long ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Molecular Species ◽  
Soft Coral ◽  
Unsaturated Fatty Acids ◽  
Total Phospholipid ◽  
Saturated Fatty Acids ◽  
Phospholipid Content ◽  
Fatty Acids Composition ◽  
Phospholipid Molecular Species

In the fatty acid composition of total lipid of the soft coral Bebryce sp., the concentration of unsaturated fatty acid predominates. The composition of saturated fatty acids is very diverse, including all saturated fatty acids from C14 to C26. The unsaturated fatty acids with high concentration are C20: 4n-6, 20:5n-3, 22:6n-3, 24:5n-6, 26:3n-6, 26:2n-6, 26:2n-7, 28:3n-6. In the fatty acids composition of the studied coral, there is presence of characterized fatty acids for the existence of sponges C25-C28 (demospongic fatty acids) with total content 29,86%. Most of the Bebryce coral species do not have zooxanthellae, therefore, in the fatty acids composition, either it is lack or contains only a small amount of markers fatty acids for zooxanthellae such as 18:5n-3, 18:4n-3, 18:3n-6, 20:4n-3. In the phospholipid content of the soft coral Bebryce sp., there is presence of characterized classes for Cnidarian animals such as phosphatidylethanolamine (PE), phosphatidylchonline (PC), phosphatidylserine (PS), phosphatidylinositol (PI), phosphonolipid is ceramide aminoethylphosphonate (CAEP). PC account for the highest concentration (37,20% of total phospholipid). The molecular species of phospholipid classes of Bebryce sp. for the first time were investigated. In the results, there we 60 phospholipid molecular species identified in 5 classes. The molecular species with high content in the classes were PE 20:4/18:1e, PE 20:4/19:1, PC 20:4/18:0e, PC 20:4/16:0e, PS 24:5/18:0e, PI 24:5/18:0, CAEP18:2base/16:0 and CAEP 18:1base/16:0.

scholarly journals Development of a Liquid Chromatography–High Resolution Mass Spectrometry Method for the Determination of Free Fatty Acids in Milk

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1548 ◽  
Author(s):  
Maroula G. Kokotou ◽  
Christiana Mantzourani ◽  
George Kokotos
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Liquid Chromatography ◽  
High Resolution Mass Spectrometry ◽  
Saturated Fatty Acids ◽  
Goat Milk ◽  
Cow Milk ◽  
Spectrometry Method ◽  
Resolution Mass

The determination of free fatty acids (FFAs) in milk is of importance for quality control, legislative purposes, authentication and product development. We present herein a liquid chromatography–high resolution mass spectrometry method for the direct determination of FFAs in milk. The method involves mild sample preparation, avoids time-consuming derivatization and allows the direct quantification of twenty-two FFAs in a 10-min single run. It was validated and applied in thirteen cow milk and seven goat milk samples. Saturated fatty acids C16:0, C18:0 and unsaturated C18:1 (n-9) were found to be the major components of milk FFAs at concentrations of 33.1 ± 8.2 μg/mL, 16.5 ± 5.3 μg/mL and 14.8 ± 3.8 μg/mL, respectively, in cow milk and at concentrations of 22.8 ± 1.8 μg/mL, 12.7 ± 2.8 μg/mL and 13.3 ± 0.3 μg/mL, respectively, in goat milk. Other saturated and unsaturated FFAs were found in significantly lower quantities. Saturated fatty acids C6:0, C8:0 and C10:0 were found in higher quantities in goat milk than in cow milk. The levels of the important (for human health) odd-chain FFAs C15:0 and C17:0 were estimated in cow and goat milk.

DEVELOPMENT OF AN EXPERIMENTAL MODEL OF AVITAMINOSIS F

2020 ◽  
Vol 20 (2) ◽  
pp. 38-40
Author(s):  
A. Levitsky ◽  
A. Lapinska ◽  
I. Selivanskaya
Keyword(s):  
Fatty Acids ◽  
Experimental Model ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Coconut Oil ◽  
The Body ◽  
Regulatory Function ◽  
Omega 3 ◽  
White Rats ◽  
Arachidonic Acids

The article analyzes the role of essential polyunsaturated fatty acids (PUFA), especially omega-3 series in humans and animals. The biosynthesis of essential PUFA in humans and animals is very limited, so they must be consumed with food (feed). Тhe ratio of omega-3 and omega-6 PUFA is very important. Biomembranes of animal cells contain about 30% PUFA with a ratio of ω-6/ ω-3 1-2. As this ratio increases, the physicochemical properties of biomembranes and the functional activity of their receptors change. The regulatory function of essential PUFA is that in the body under the action of oxygenase enzymes (cyclooxygenase, lipoxygenase) are formed extremely active hormone-like substances (eicosanoids and docosanoids), which affect a number of physiological processes: inflammation, immunity, metabolism. Moreover, ω-6 PUFA form eicosanoids, which have pro-inflammatory, immunosuppressive properties, and ω-3 PUFAs form eicosanoids and docosanoids, which have anti-inflammatory and immunostimulatory properties. Deficiency of essential PUFA, and especially ω-3 PUFA, leads to impaired development of the body and its state of health, which are manifestations of avitaminosis F. Prevention and treatment of avitaminosis F is carried out with drugs that contain PUFA. To create new, more effective vitamin F preparations, it is necessary to reproduce the model of vitamin F deficiency. An experimental model of vitamin F deficiency in white rats kept on a fat –free diet with the addition of coconut oil, which is almost completely free of unsaturated fatty acids, and saturated fatty acids make up almost 99 % of all fatty acids was developed. The total content of ω-6 PUFA (sum of linoleic and arachidonic acids), the content of ω-3 PUFA (α-linolenic, eicosapentaenoic and docosahexaenoic acids) in neutral lipids (triglycerides and cholesterol esters) defined. Тhe content of ω-6 PUFA under the influence of coconut oil decreased by 3.3 times, and the content of ω-3 PUFA - by 7.5 times. Тhe influence of coconut oil, the content of ω-6 PUFA decreased by 2.1 times, and the content of ω-3 PUFA - by 2.8 times. The most strongly reduces the content of ω-3 PUFA, namely eicosapentaenoic, coconut oil, starting from 5 %. Consumption of FFD with a content of 15 % coconut oil reduces the content of eicosapentaenoic acid to zero, ie we have an absolute deficiency of one of the most important essential PUFAs, which determined the presence of vitamin F deficiency.

EFFECT OF GINGER, LEMURU FISH OIL SAPONIFICATION AND OLIVE OIL ON COMPOSITION FATTY ACID OF BEEF

2014 ◽  
Vol 4 (1) ◽  
pp. 31-39
Author(s):  
Siwitri Kadarsih
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Olive Oil ◽  
Rice Bran ◽  
Dry Matter ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Omega 3 ◽  
Omega 6

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords : 

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