scholarly journals An Evaluation of The Antifungal and Antioxidant Activity of Pinus merkusii Bark Ethyl Acetate Extract

2021 ◽  
Vol 15 (1) ◽  
pp. 102-110
Author(s):  
Masendra Masendra ◽  
Brandon Aristo Verick Purba Purba ◽  
Ganis Lukmandaru
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Growth Inhibition ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Compounds ◽  
Potential Source ◽  
Antioxidant Agents ◽  
Pinus Merkusii ◽  
Ethyl Acetate Extracts

Pinus merkusii is the only species in the Pinaceae family to grow in Southeast Asia, including Indonesia, and serves as a source of wood and oleoresins. During the utilization of this pine, the bark is discarded as residue, however, this residue is possibly a potential source of antifungal and antioxidant agents, due to the polyphenol contents. This study, therefore, aims to investigate the antifungal and antioxidant compounds present in the bark of P. merkusii. The ethyl acetate extracts of Pinus merkusii were fractionated to obtain five fractions (Fr. A- Fr. E) and the bioactivity of each fraction was observed. Subsequently, the growth inhibition against Phanerochaete chrysosporium, was evaluated for antifungal activity, while DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to ascertain antioxidant activity. According to the GC-MS analysis, the ethyl acetate fractions contain triterpenoids and steroids as the dominant compounds as well as minor amounts of mono-sesquiterpenoids and hydrocarbons. The highest growth inhibition exhibited by Fr. C- Fr. E (>60%) indicates triterpenoids and steroids act as a fungal inhibitors. Meanwhile, the five fractions exhibited low antioxidant activity because the steroids and triterpenoids present are possibly ineffective DPPH inhibitors. Also, the antifungal and antioxidant activity R2 values were discovered to correlate 0.3, indicating a low positive correlation.

scholarly journals KAJIAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN TEMBELEKAN (Lantana camara L) BERDASARKAN TINGKAT KEPOLARAN PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (3) ◽  
Author(s):  
Oktavian Mangela ◽  
Ahmad Ridhay ◽  
Musafira Musafira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Antioxidant Compounds ◽  
Ic50 Value

The investigation about the antioxidant activity of tembelekan (Lantana camara L) leaf extract based on level polar solvent . Has been done the aim of this study was to determine the IC50 value and to know the content of antioxidant compounds from tembelekan leaf extract based on the level of polar solvent the extraction of tembelekan leaf was done with maceration method . The result showed that ethyl acetate extract of tembelekan leaf has the highest antioxidant activity with IC50 value was 71,70 ppm. Keywords: Tembelekan ( Lantana Camara L ) , IC50 , Antioxidant , DPPH

scholarly journals Antibacterial and antifungal activity of crude extracts of Plumeria rubra L.

2013 ◽  
pp. 87-94
Author(s):  
Laila Jarin ◽  
Md Shafiqur Rahman ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts ◽  
Plumeria Rubra

Petroleum ether, carbon tetrachloride, chloroform and ethyl acetate extracts of Plumeria rubra leaves were studied for their antimicrobial activities against eleven human pathogenic bacteria, viz., Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae and four human pathogenic fungi, viz., Aspergillus niger, A. ochraceus, A. ustus and Candida albicans using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extract exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extracts exhibited the largest zone of inhibition (25 mm in diameter with 2000 mg/disc extract) against E. coli. The highest inhibition of fungal radial mycelial growth (62.00% with 100 mg extract/ml medium) was recorded against A. ustus with ethyl acetate extract. The MICs were determined by broth macrodilution technique. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against E. coli. However, for fungi the lowest MIC was 500 mg/ml against A. ustus with the same extract. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13409 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):87-94, 2008

scholarly journals ANTIBACTERIAL ACTIVITY OF FRACTIONS OF ETHYL ACETATE EXTRACT OF GARCINIA LATTISSIMA MIQ. FRUITS

2017 ◽  
Vol 10 (17) ◽  
pp. 81
Author(s):  
Nenengsiti Silfi Ambarwati ◽  
Amarila Malik ◽  
Ageng Tri Listari ◽  
Nirwana Nirwana ◽  
Berna Elya ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Antibacterial Agents ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Agar Medium ◽  
Bacterial Drug Resistance ◽  
Ethyl Acetate Extracts

Objectives: The emergence of new infections and increase in bacterial drug resistance has created a serious need for the expansion of new antibacterial agents from natural sources. The study was carried out to evaluate the antibacterial activity of fractions of ethyl acetate extract of Garcinia latissima Miq. fruits.Methods: The fractionation was done using a silica gel column and organic solvents as the eluent, i.e., n-hexane, ethyl acetate, and methanol. All fractions were assayed for antibacterial activity, which was done by performing disc diffusion for growth inhibition against Bacillus subtilis and Pseudomonas aeruginosa. In addition, the growth inhibition activity was also examined by performing bioautography assay using pre-coated silica gel 60 GF 254 plates as the stationary phase. Fractions A-F were eluted using n-hexane:chloroform (1:4), while Fractions G-K were used ethyl acetate:dichloromethane (4:1) as the mobile phase. The plate was visualized by ultraviolet at λ 254 nm and 366 nm, while the other one was contacted with the inoculated agar medium to observe zone inhibition. Further, the minimum inhibitory concentration (MIC) value was determined by performing microdilution.Results: The result showed that the antibacterial activity of all fractions was more active at inhibiting the growth of B. subtilis than P. aeruginosa, mainly for Fractions H and J. However, the strongest antibacterial activity was showed by Fractions H and J against B. subtilis, MIC 312.5 μg/mL (lower than reference, which is erythromycin antibiotic (25 μg/mL), followed by Fraction D against B. subtilis MIC 625 μg/mL, Fraction K against P. aeruginosa MIC 625 μg/mL, whereas Fractions C, E, and G against B. subtilis, and Fraction E against P. aeruginosa also showed low MIC values (1.250 μg/mL).Conclusions: The results indicated that fractions of G. latissima Miq. fruit ethyl acetate extracts possessed antibacterial activity. The most active fraction that inhibited the growth of B. subtilis was shown by Fractions H and J; these fractions have the potential to be developed as new antibacterial agents.

scholarly journals Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa

2020 ◽  
Vol 41 (4) ◽  
Author(s):  
Ton That Huu Dat ◽  
Phung Thi Thuy Oanh ◽  
Vu Thi Thanh Tam ◽  
Hoang Le Tuan Anh
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Antioxidant Agents ◽  
Antimicrobial And Antioxidant Activity ◽  
Ethyl Acetate Extracts

Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.       

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN GOLONGAN FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN PRANAJIWA (Euchresta horsfieldii Lesch Benn.)

2017 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Ni Wayan Rika Kumara Dewi ◽  
I Wayan Gunawan ◽  
Ni Made Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Infrared Spectra ◽  
Ethyl Acetate Extract ◽  
Total Flavonoid ◽  
Separation And Purification ◽  
Ic50 Value ◽  
Ethyl Acetate Extracts ◽  
In Vitro Antioxidant Activity

ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat FE) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3’,4’-pentahidroksi flavonol.   ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant activity was carried out using DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. The separation of the ethyl acetate extracts was conducted by column chromatography and the identification by using FTIR and UV-Vis spectrophotometer. The results showed that ethyl acetate extract has antioxidant activity with IC50 value of 393,95 µg/mL and the total flavonoid of 6619,72 mg QE /100g or 6,62% QE. The separation and purification of  ethyl acetate extract was chromatography column on silica gel 60 and the solvent n-hexane:chloroform:ethanol (20:1:1) based on UV-Vis and Infrared spectra positive flavonoid isolate (isolates FE) was tentaviely identified as 3,5,7,3',4'-pentadihydroxy flavonols.    

scholarly journals Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Ton That Huu Dat ◽  
Phung Thi Thuy Oanh ◽  
Vu Thi Thanh Tam ◽  
Hoang Le Tuan Anh
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Antioxidant Agents ◽  
Antimicrobial And Antioxidant Activity ◽  
Ethyl Acetate Extracts

Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.       

scholarly journals POTENSI ANTIOKSIDAN METABOLIT SEKUNDER FRAKSI ETIL ASETAT DARI KULIT BATANG EBONI (Diospyros celebica Bakh.)

KOVALEN ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 155-165
Author(s):  
Vivi Sasmita ◽  
Ahmad Ridhay ◽  
Hardi Ys
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Spectral Data ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
Wave Number ◽  
Antioxidant Compounds ◽  
Ic50 Value

Research on the antioxidant potential of the secondary metabolite compound, ethyl acetate from ebony bark (Diospyros celebica Bakh.) has been conducted. This study aims to characterize and determine the antioxidant activity of secondary metabolites of ethyl acetate fraction from ebony bark. Ethyl acetate extract was fractionated using Liquid Vacum Chromatography (KVC) and Gravity Column Chromatography (KKG). The isolated compounds were characterized using UV-Vis and IR spectrophotometers. Data on maximum absorption of λmax 273 nm from UV-Vis analysis showed the presence of chromophore phenol. IR spectral data showed several specific peaks, among others, at wave number (ʋ) of 3425.58 cm-1 indicating the presence of –OH group, C-H aliphatic bond at ʋ of  2924.09 cm-1  and 2854.65 cm-1, and the peak at ʋ of 1604.77 -1465.90 cm-1 and 3078.39 cm-1 indicated the aromatic group, reinforcing the assumption that the isolates produced were phenolic compounds. IC50 value of 75.47 µg/mL from antioxidant activity testing showed that the isolates obtained were antioxidant compounds with strong antioxidant categories. Keywords: antioxidant, Diospyros, ebony, phenolic, isolation

scholarly journals Flavon Compound from The Ethyl Acetate Extract of The Stem of Supit (Tetracera indica Merr.)

Molekul ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. 38
Author(s):  
Muharni Muharni ◽  
Elfita Elfita ◽  
Riska Adillah ◽  
Heni Yohandini ◽  
Julinar Julinar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Spectroscopic Analysis ◽  
Ethyl Acetate Extract ◽  
Stone Disease ◽  
Antioxidant Compound ◽  
Dpph Method ◽  
Isolated Compound ◽  
Ethyl Acetate Extracts

Tetracera indica Merr. in Musi Banyuasin, is one of the traditional medicine used by the community for the treatment of kidney stone disease and gout, but this claim is not recorded in the treatment of kidney stones and gout in Indonesia.  In this study, isolation of antioxidant compound from ethyl acetate extracts of supit (Tetracera indica) was done. The isolation was carried out through step gradient polarity extraction, and separated and purified by chromatography technique. The determination of the structure of the isolated compound was performed by spectroscopy method including UV, IR, and NMR 1D and 2D, and antoxidant activity was determined  by DPPH method.  An  active antioxidant compound was isolated from ethyl acetate extract in form of yellow solid (15 mg).  Based on spectroscopic analysis the isolated compound was 5,8-dihydroxy-7-methoxyflavone.  The compound showed strong antioxidant activity (IC50 8.25 μg/mL) higher than standard ascorbic acid (IC50 11.3 μg/mL).  This data concluded that efficacy of supit (Tetracera indica) for the treatment related to antioxidant activity (uric acid) is proven by the identification of one antioxidant compound of this plant.

scholarly journals Aktivitas Antioksidan dan Fitokimia dari Ekstrak Etil Asetat Pucuk Idat (Cratoxylum glaucum)

2018 ◽  
Vol 5 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Natural Antioxidant ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidants ◽  
Synthetic Antioxidant ◽  
Phytochemical Content

Neutralizing free radicals can use both synthetic and natural antioxidant compounds. Although synthetic antioxidant compounds are more active, but lately the use of synthetic antioxidants is being reduced because they are reported to have carcinogenic side effects. Natural antioxidant compounds found in plants are phenolic groups such as flavonoids, tannins, xanthones, and anthraquinones. This compound is widely found in the genus Cratoxylum where one of the species is pucuk idat (Cratoxylum glaucum). Pucuk idat are often used by people of Bangka as a flavoring dish and are believed to be traditional medicine to facilitate breastfeeding, tighten skin, treat fever, cough, and diarrhea. The increasing of antioxidants needs and lots efficacy of pucuk idat, hence this study aims to determine the antioxidant activity of ethyl acetate extract Cratoxylum glaucum. The antioxidant test in this study used the DPPH (Diphenylpicrylhydrazyl) method and vitamin C as a positive control. The results of this study indicate that the extract of ethyl acetate Cratoxylum glaucum has strong antioxidant activity with value IC50 32,213 μg / mL. Phytochemical content itself includes hydroquinone phenols (tannins), flavonoids, and steroids. This shows that ethyl acetate extract Cratoxylum glaucum can be used as natural antioxidant.

scholarly journals Antioxidant and Antimicrobial Activity of Mexican Oregano (Poliomintha longiflora) Essential Oil, Hydrosol and Extracts from Waste Solid Residues

Plants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 22 ◽  
Author(s):  
Teresa Cid-Pérez ◽  
Raúl Ávila-Sosa ◽  
Carlos Ochoa-Velasco ◽  
Blanca Rivera-Chavira ◽  
Guadalupe Nevárez-Moorillón
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Chemical Components ◽  
Waste Solid ◽  
Ethyl Acetate Extracts

Poliomintha longiflora is a Mexican oregano, which has not been widely studied. This work aimed to describe the chemical composition, antimicrobial and antioxidant activities present in P. longiflora essential oil (EO), the hydrosol from EO extraction and extracts from waste solid residues (WSRs), identified as ethanol extract, ethyl acetate extract and the subfractions of ethanol and ethyl acetate extracts. The chemical characterization of the EO, hydrosol and WSR extracts was performed by GC–MS and HPLC. Their antioxidant activity was evaluated using two methods, and their antimicrobial activity was evaluated against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, and Salmonella Typhimurium. Thirty-one chemical components were identified in the EO. The subfractions from the ethanol and ethyl acetate extracts contain methylmaleic anhydride, thymoquinone, thymol, carvacrol, thymol acetate, carvacrol acetate, and phenolic acids. The EO presented the highest biological activities for antioxidant (136.05 mg equivalent of ascorbic acid/g (AAE/g); IC50 83.70 μg/mL of 2, 2-diphenyl-1-picrylhydrazyl (DPPH)) and antimicrobial tests (minimal inhibitory concentration (MIC) value of 250–750 mg/L), while the hydrosol and the ethyl acetate extract from WSRs had the lowest antioxidant activity (14.16 and 12.29 mg AAE/g respectively), and the hydrosol had the lowest antimicrobial activity (MIC of 3000 mg/L). The data suggest that Mexican oregano P. longiflora hydrosol and extracts from waste solid residues can still have compounds with antimicrobial and antioxidant capacities.

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