Assessment of Candida Species Colonization and Denture-Related Stomatitis in Bar- and Locator-Retained Overdentures

2014 ◽  
Vol 40 (5) ◽  
pp. 549-556 ◽  
Author(s):  
Kerem Kilic ◽  
Ayse Nedret Koc ◽  
Fatma Filiz Tekinsen ◽  
Pinar Yildiz ◽  
Duygu Kilic ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Hygiene ◽  
Significant Relationship ◽  
Candida Species ◽  
Smoking Habit ◽  
Morphological Characteristics ◽  
Clinical History ◽  
Common Species ◽  
Hygiene Practices ◽  
Edentulous Patients

The aim of this study was to assess the prevalence of denture-related stomatitis (DRS) in different attachment-retained overdenture wearers and its association with particular colonizing Candida species. Thirty-seven edentulous patients with implant-supported maxillary or mandibular overdentures were enrolled. A full clinical history was obtained, including details of patients' oral hygiene practices and the levels of erythema based on Newton's classification scale. Swabs were taken from the palate and investigated mycologically to identify the yeast colonies. Quantitative and qualitative microbiological assessments were performed, which included recording the total numbers of colonies (cfu), their color, and their morphological characteristics. Significant differences were found in cfu values between the attachment and inner surfaces of locator- and bar-retained overdentures (P < .05). Candida albicans was the most common species in both evaluations, being isolated from 81.3% of bar-retained overdentures and 38.1% of locator-retained overdentures. DRS developed in all patients using bar-retained overdentures but in only 71.4% of those using locator-retained overdentures. No statistically significant relationship was found between bar and locator attachments according to smoking habit, overnight removal, or plaque and gingival indices (P > .05).

scholarly journals Species identification of Candida isolated from clinical specimens in a tertiary care hospital

2016 ◽  
Vol 9 (1) ◽  
pp. 20 ◽  
Author(s):  
Lsmet Nigar ◽  
Shirin Tarafder ◽  
Rehana Razzak Khan ◽  
S. M. Ali Ahmed ◽  
Ahmed Abu Saleh
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Tertiary Care ◽  
Clinical Manifestations ◽  
Common Species ◽  
Blood Stream ◽  
Care Hospital ◽  
Emerging Pathogens ◽  
Predisposing Factor ◽  
Clinical Specimens

<p><strong>Background:</strong> Candida species are responsible for various clinical manifestations from mucocutaneous overgrowth to blood stream infections especially in immunocompromized situations. Although C. albicans is the most prevalent species, high incidence of non-albicans Candida species with antifungal resistance are emerging which is posing a serious threat to the patients care.</p><p><strong>Objective:</strong> This study aimed to isolate and identify different species of Candida from different clinical specimens. Methods: A total of 100 different clinical specimens were studied of which 35 were oral swab, 28 were high vaginal swab, 15 were urine, 14 were nail, 04 were bronchoalveolar lavage and peritoneal fluid were 04. Among 100 clinical specimens, Candida isolates were identified in 64 specimens. Isolation of Candida species was done by primary culture in SDA. Subsequent identification of species were performed by germ tube test, subculture in chromo­genic agar medium and carbohydrate assimilation test with commonly used twelve sugars.</p><p><strong>Results:</strong> Out of 64 isolated Candida species, Candida albicans were 51.56% and the non-albicans Candida species were 48.44%. The most prevalent Candida species was C. albicans 33 (51.53%) followed by C. tropicalis 17 (26.56%). C. glabrata 4 (6.25%), C. parapsilo­sis 4 (6.25%), C. krusei 3 (4.68%) and C. guilliermondii 2 (3.2%). One of the isolated Candida species was unidentified.</p><p><strong>Conclusion:</strong> Though Candida albicans was found as the most common species, but non-albicans Candida species are appearing as emerging pathogens as well. Exposure to chemotherapy appeared to be the commonest predisposing factor for Candida infection followed by indwelling urinary catheter in situ for prolong period.</p>

Salivary Candida, Caries and Candida in Toothbrushes

2012 ◽  
Vol 37 (2) ◽  
pp. 167-170 ◽  
Author(s):  
T Ratson ◽  
R Bar-Ness Greenstein ◽  
Y Mazor ◽  
B Peretz
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
School Children ◽  
Significant Relationship ◽  
Candida Species ◽  
Caries Experience ◽  
Oral Microbiota ◽  
Dental Clinic ◽  
Positive Growth

Background: Candida species are common inhabitants of the normal oral microbiota. A few studies founded a relationship between high levels of Candida albicans in the oral cavity and high DMF scores. Toothbrushes can also be reservoirs of microorganisms, the proliferation of these microorganism on a toothbrush could be a major factor for its distribution in the oral cavity. Aim: To examine the associations between salivary Candida and DMF, and between salivary Candida and Candida in the toothbrush. Method: 46 healthy school children, who attended a University pediatric dental clinic, were tested for Candida in their saliva and in their toothbrush. Their DMF was recorded. Results: 38 children were Candida-positive (79.2%), out of whom 5 demonstrated a positive growth of Candida in the toothbrushes. No correlation was found between Candida in the saliva and in the toothbrush. The number of Candida-positive girls was significantly higher than the number in boys. No significant relationship between caries experience and the presence of Candida was found. Conclusions: No correlation was found between Candida in the saliva and in the toothbrush. The origin of the Candida in the toothbrush is not totally clear.

scholarly journals ORAL CANDIDA ALBICANS COLONIZATION RATE IN FIXED ORTHODONTICS PATIENTS

2020 ◽  
Author(s):  
Hussein Mohammad Shoga Al-deen ◽  
Ahmed Ali Obeyah ◽  
Hassan Abdulwahab Al-Shamahy ◽  
Ibrahim Zaid Al-Shami ◽  
Manal Ahmad Saleh AL-amri ◽  
...  
Keyword(s):  
Risk Factors ◽  
Candida Albicans ◽  
Peer Review ◽  
Oral Hygiene ◽  
Candida Species ◽  
Demographic Data ◽  
Oral Rinse ◽  
Orthodontic Patients ◽  
Oral Candida ◽  
E Mail

Objectives:  The objective of this study was to assess the oral Candida albicans colonization ( OCAC)  in a cluster of teenagers and young adults while being treated with a fixed orthodontic appliance (FOA). Subjects and methods:  The investigational group was selected from orthodontic patients whom were examined clinically as soon as to get baseline information before active treatment.  The cluster included 210 patients; 45 males, 165 females (mean age 21.6 ± 4.5 years).  Clinical, demographic data and risk factors were collected in standard questionnaire then each individual was directed to carry out oral wash by a phosphate-buffered saline solution, which was expectorated and processed intended for the isolation of Candida species on Sabouraud’s dextrose agar. The isolated Candida species were identifying by culturing on chromogenic Candida agar and notice species-specific colony natures. Results:  The predominant Candida species isolated was C. albicans with OCAC rate equal to 13.8% extensively enhanced after the insertion of a FOA, as revealed by the oral rinse (P < 0.05) techniques. The results also revealed an increase of OCAC in male patients (24.4%) than female patients (10.9%),   21-25 years patients (17.1%), and regular smoking and Qat chewing were significant associated risk factors (OR=28.6, OR=10.7 respectively , P < 0.0001).  There was no significant association between C. albicans  colonization with oral hygiene in fixed Orthodontic patients. Conclusion:  As a whole, the current data suggest that the introduction of FOA is likely to promote OCAC. Moreover, it becomes visible that the routine oral hygiene procedures performed by these patients may not necessarily reduce OCAC while smoking and chewing Qat habits significantly increased OCAC in FOA. Also smoking and Qat chewing during FOA treatment should be banned if potential harmful effects are to be prevented. Further work with a larger sample size is required to confirm or deny these results. Peer Review History: Received 27 March 2019; Revised 20 April; Accepted 8 May, Available online 15 May 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.5/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Name: Dr. A.A. Mgbahurike Affiliation: University of Port Harcourt, Nigeria E-mail: [email protected]   Name: Dr. Tamer Mohamed Ali El Habibi Affiliation: Suez Canal University, Egypt E-mail: [email protected]   Comments of reviewer(s): Similar Articles: ORAL C. ALBICANS COLONIZATION AND NON-CANDIDA ALBICANS CANDIDA COLONIZATION AMONG UNIVERSITY STUDENTS, YEMEN PREVALENCE AND ASSOCIATED FACTORS OF ORAL NON-CANDIDA ALBICANS CANDIDA CARRIAGE IN DENTURE WEARERS IN SANA'A CITY- YEMEN  

scholarly journals Evaluation of the Effect of Contraceptives on Prevalence of Candida Species on Vaginal Candidiasis in Dhaka, Bangladesh

2016 ◽  
Vol 1 (2) ◽  
pp. 61-64 ◽  
Author(s):  
Md. Abdullah Yusuf ◽  
Md. Abul Quashem Chowdhury ◽  
Abu Naser Ibne Sattar ◽  
Md. Mostaqimur Rahman
Keyword(s):  
Oral Contraceptive ◽  
Candida Species ◽  
Strong Association ◽  
Clinical History ◽  
Common Species ◽  
Vaginal Candidiasis ◽  
P Value ◽  
College Hospital ◽  
Oral Contraceptive Pills ◽  
Detailed Clinical History

This study was designed to find out the relationship between uses of different contraceptives and the prevalence of vaginal candidiasis. High vaginal swabs were obtained from 350 women in the age group of 15-45 years with vaginal discharge attending at Sir Salimullah Medical College Hospital, Dhaka, which comprised of 247 contraceptive users & 103 non-contraceptive users. A detailed clinical history and a thorough examination of all the cases were done. After making the clinical diagnosis, appropriate tests for diagnosing Candidiasis were done by using standard procedures. Of the 247 women of contraceptive users, Candida species was isolated in 140 (56.7%) cases compared to 32 (31.1%) from 103 non-contraceptive users (p value <0.001). Different species of Candida were isolated from 172 (49.1%) cases among 350 women of which C. albicans was found in 125 (72.7%) cases, C. glabrata in 29 (16.9%) cases, C. tropicalis in 13 (7.5%), and C. krusei in 5 (2.9%) cases. Among 173 oral contraceptive users, the isolation rate of Candida species was 120 (69.4%). This was followed by injectables (17, 12.2%) and IUCD (03, 2.1%) cases (p value <0.05). The study revealed a strong association between use of contraceptives and the prevalence of vaginal infection by Candida species of which C. albicans was the most common species (72.7%). Among all contraceptives, use of oral contraceptive pills (OCP) was found as the most common cause of vaginitis, next to which were injectables and IUCDs.Bangladesh J Med Microbiol 2007; 01 (02): 61-64

scholarly journals Molecular Identification and Hemolytic Activity of Candida Species Isolated from Urine of Healthy and Diabetic Women in Kurdistan of Iraq

2020 ◽  
Vol 8 (1) ◽  
pp. 1-6
Author(s):  
Zinah S. Oufi ◽  
Ahmed B. Mohammed ◽  
Samir Kh. Abdullah
Keyword(s):  
Candida Albicans ◽  
Molecular Identification ◽  
Hemolytic Activity ◽  
Candida Species ◽  
Agar Medium ◽  
Age Groups ◽  
Common Species ◽  
Candida Infection ◽  
Urine Samples ◽  
Pichia Kudriavzevii

A survey was carried out on the incidence of Candida yeasts in urine of healthy (non-diabetic) and diabetic women in Duhok and Zakho cities from February 2019 to April 2019. Out of 620 urine samples examined, 144 samples were found positive for Candida infection (23.2%). There were significant differences in incidence of Candida infection between urine of healthy and diabetic women as well as among age groups. All Candida isolates from positive samples were identified by using Chromogenic Candida Agar medium and confirmed by sequencing of the ITS1 and ITS4 region of rDNA. The identified Candida species were C.albicans, C.glabrata, C.krusei and C.tropicalis. The most common species was C.albicans (34%), followed by C.glabrata (29.9%), then C.tropicalis (13.8%), whereas, C.krusei (=Pichia kudriavzevii) displayed the least incidence (9.7%). All tested strains (n=83) for their hemolytic activity on Sabouraud's dextrose agar amended with sheep blood and glucose showed positive activity. Candida albicans and C.tropicalis displayed the highest hemolytic activity.

scholarly journals Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

2021 ◽  
pp. 1608-1614
Author(s):  
Nadezhda Sachivkina ◽  
Irina Podoprigora ◽  
Dmitry Bokov
Keyword(s):  
Candida Albicans ◽  
Phosphate Buffer ◽  
Candida Species ◽  
Candida Glabrata ◽  
Phosphate Buffer Solution ◽  
Morphological Characteristics ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Buffer Solution ◽  
Solution Ph

Background and Aim: Different Candida species isolated in humans and animals have different types of parasite activity. The most pathogenic species is Candida albicans followed by Candida tropicalis. However, the effects of the morphology of Candida krusei, Candida guilliermondii, and Candida glabrata biofilms on the pathogenicity of these species have not been fully characterized. To the best of our knowledge, there is no literature on the effect of farnesol on rare Candida species. This study aimed to check the effect of different farnesol concentrations on the species C. krusei, C. guilliermondii, and C. glabrata compared with the strain C. albicans ATCC 10231, which has been widely studied, and is a strong producer of biofilms. Materials and Methods: We studied the morphological and densitometric parameters of biofilms produced by Candida species under the influence of the drug farnesol (Sigma-Aldrich, St. Louis, MO). We used a heart brain broth with the addition of 2% bovine blood serum in 96-well plates. To each well, we added 100 μL of C. albicans, C. krusei, C. guilliermondii, or C. glabrata culture, and 0.2-400 μM farnesol. The microliter plates were cultured with the lid closed at 37°C for 48 h. Then, the liquid was removed, and the wells were washed 3 times with 200 μL phosphate buffer solution (pH 7.3). Biofilm fixation was performed using 150 μL of 96% ethanol for 15 min. Then, the microliter plates were dried for 20 min at 37°C, a 0.5% solution of crystalline violet was added, and the plates were placed in an incubator at 37°C. After 5 min, the contents of the wells were removed, washed 3 times with 200 μL of phosphate buffer solution (pH 7.2), and dried. The dye was extracted by washing with 200 μL of 96% ethanol for 30 min. The results were obtained using a photometric analyzer of enzyme immunoassay reactions at an optical density (OD) wavelength of 450 nm. Results: All of Candida spp. strains tested were susceptible to farnesol at concentrations ranging from 0.8 to 400 μM for C. albicans, C. krusei, and C. guilliermondii, and 12.5 to 400 μM for C. glabrata. Conclusion: This study provides new insights into the use of farnesol against biofilms produced by Candida species, but further studies in vivo are necessary to evaluate the effectiveness of the reduction of OD. To the best of our knowledge, the antimicrobial activity of farnesol against C. krusei, C. guilliermondii, and C. glabrata has not been reported previously, although studies have confirmed the inhibitory effect of farnesol on the growth of different microorganisms.

Effect of oral anticipatory guidance on oral health and oral hygiene practices in preschool children

2006 ◽  
Vol 30 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Débora Conçalves Farias ◽  
Soraya Coelho Leal ◽  
Orlando Ayrton de Toledo ◽  
Jorge Faber ◽  
Ann Cristina Barreto Bezerro
Keyword(s):  
Oral Health ◽  
Oral Hygiene ◽  
Dental Plaque ◽  
Intervention Group ◽  
Control Group ◽  
Health Habits ◽  
Hygiene Practices ◽  
Dental Office ◽  
And Gender ◽  
Group Control Group

Thirty children (6 to 18 months), enrolled in a dental program since birth, make up the intervention group. Control group consisted of thirty children who were of similar age and gender but had never been to a dental office. The presence of visible dental plaque and the performance of oral hygiene differed statistically between both groups. The dental program was effective in reducing visible dental plaque, and in establishing oral health habits among parents and young children.

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