Phagocytic activity of blood monocytes in response to methicillin-resistant strains of Staphylococcus aureus

Current study performed to estimate the phagocytic activity of blood monocytes of varying phenotypes exposed to MRSA and MSSA strains. Objects: Blood monocytes were collected from 25 healthy adults (age: 25–45 years). Live suspensions of MRSA/MSSA strains were used at concentration of 106 colony-forming units (CFU)/mL. Metods. Phagocytic functions were estimated by using fluorescein isothiocyanate (FITC)-labelled MRSA and MSSA strains followed by running flow cytometry on FC 500 series flow cytometer (Beckman Coulter, USA). Whole peripheral blood cells were directly labelled with immunofluorescently tagged monoclonal CD14-PE/CD45-ECD/HLA-DR-PC5/CD16-PC7 antibodies (Beckman Coulter, USA). Respiratory burst intensity was evaluated in monocytes by measuring activity of lucigeninand luminol-dependent spontaneous and induced chemiluminescence. Monocytes were induced by using live suspension of MRSA/MSSA strains at a concentration of 106 CFU/mL. Results and discussion. While studying luminol-dependent monocyte activities after exposure to MRSA vs. MSSA, it was observed a 3.5-fold decreased curve square, whereas lucigenin-dependent chemiluminescence was increased by 6-fold. Compared to MSSA exposure, index of activation (IA) was decreased by 1.1-fold in response to MRSA exposure that was confirmed by lowered release of reactive oxygen species (ROS) from monocytes in response to MRSA exposure. Moreover, IRSS increased by 1.3-fold upon MRSA exposure. Examining monocyte oxygen-independent phagocytosis against MRSA vs. MSSA revealed significantly increased phagocytic number and concomitantly decreased phagocytic index. An evaluation of the activities of various monocyte subsets in response to MRSA vs. MSSA revealed increased phagocytic index by 1.5-fold for CD14lowCD16+ and CD14+CD16+ monocyte subsets as well as 3-fold for CD14+CD16– monocytes. Counts for all phagocytic subsets were decreased (1.4-, 1.5- and 4-fold for CD14lowCD16+, CD14+CD16+ and CD14+CD16– monocytes, respectively). To summarize, intensity of the respiratory burst was lowered upon MRSA exposure and percentage of monocyte subsets. Overall deficiency of superoxide anion production was observed in response to MRSA. In contrast, oxygen-independent event revealed phenotypic changes in frequency of peripheral blood monocytes upon MRSA exposure. We observed that CD14+CD16– classical monocytes were more rapidly activated. Conclusion. Thus, we concluded that CD14+CD16– monocytes became more rapidly activated but exhibited less effective phagocytosis, whereas CD14+CD16+ and CD14lowCD16+ monocytes were more slowly activated and demonstrated stronger phagocytic activity.

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Evaluation of the phagocytic activity of peripheral blood monocytes of patients with Jorge Lobo's disease

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822004000200010 ◽

2004 ◽

Vol 37 (2) ◽

pp. 165-168 ◽

Cited By ~ 9

Author(s):

Fátima Regina Vilani-Moreno ◽

Luciana Moreira Silva ◽

Diltor Vladimir Araújo Opromolla

Keyword(s):

Incubation Time ◽

Peripheral Blood ◽

Phagocytic Activity ◽

Healthy Subjects ◽

Mononuclear Cells ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Significant Difference ◽

Host Parasite ◽

And Control

Studies on host-parasite interaction in Jorge Lobo's disease are scarce, with no report in the literature on the phagocytosis of Lacazia loboi by phagocytic mononuclear cells. Thus, the objective of the present study was to assess the phagocytic activity of blood monocytes in the presence of L. loboi in patients with the disease and in healthy subjects (controls) over 3 and 24 hours of incubation. Statistical analyses of the results showed no significant difference in percent phagocytosis of the fungus between patient and control monocytes. With respect to incubation time, however, there was a significant difference, in that percent phagocytosis was higher at 3 hours than at 24 hours (p <0.01). These results suggest that monocytes from patients with the mycosis are able to phagocyte the fungus, as also observed in control individuals.

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Increased phagocytic activity of peripheral blood monocytes after intravenous injection of phospholipase A2 to monkeys

Immunology Letters ◽

10.1016/0165-2478(91)90120-y ◽

1991 ◽

Vol 28 (1) ◽

pp. 5-9 ◽

Cited By ~ 2

Author(s):

Alejandro Bravo-Cuellar ◽

Françoise Homo-Delarche ◽

Rodolfo Ramos-Zepeda ◽

Pierre Dubouch ◽

Jeannine Cabannes ◽

...

Keyword(s):

Phospholipase A2 ◽

Intravenous Injection ◽

Peripheral Blood ◽

Phagocytic Activity ◽

Blood Monocytes ◽

Peripheral Blood Monocytes

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Cell surface antigen expression by peripheral blood monocytes in allergic asthma: results of 2.5 years therapy with inhaled beclomethasone dipropionate

Mediators of Inflammation ◽

10.1155/s096293519600052x ◽

1996 ◽

Vol 5 (5) ◽

pp. 362-369

Author(s):

W. A. T. Slieker ◽

P. Th. W. van Hal ◽

J. M. Wijkhuijs ◽

J. P. M. Hopstaken-Broos ◽

J. A. Noordhoek ◽

...

Keyword(s):

Cell Surface ◽

Allergic Asthma ◽

Peripheral Blood ◽

Beclomethasone Dipropionate ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Hla Dr ◽

Long Term Treatment ◽

Inhaled Glucocorticoids

At present, inhaled glucocorticoids are widely accepted as the therapy of choice in chronic asthma. Treatment with inhaled glucocorticoids significantly suppresses local airway inflammation in asthmatics, but may also have systemic effects, e.g. a reduction of the number of circulating hypodense eosinophils or a down-modulation of HLA-DR antigen (Ag) expression by T lymphocytes in peripheral blood. However, the effect of long-term therapy with inhaled glucocorticoids on peripheral blood monocytes (PBM), which are the precursors of the most numerous cell type in the lung, the alveolar macrophage, have not yet been evaluated. We therefore investigated the expression of various cell surface Ag on PBM from non-smoking patients with allergic asthma who were treated for 2.5 years with a β2-receptor agonist plus either an inhaled glucocorticoid (beclomethasone dipropionate, BDP) (n= 4) or an anticholinergic or placebo (n= 8). We compared the results with healthy volunteers (n= 7). Long-term treatment of allergic asthmatics with inhaled BDP, but not anticholinergic or placebo therapy, was associated with a significantly lower CDllb Ag expression (p< 0.04) and higher expression of CD13, CD14 and CD18 Ag (p< 0.05,p< 0.02 andp< 0.04, respectively) when compared with the healthy control subjects (n= 7). Most interestingly, PBM of asthmatics treated with inhaled BDP expressed an almost two-fold higher level of CD14 Ag on their cell surface than PBM of patients treated with anticholinergic or placebo (p< 0.03). No significant differences in the expression of CD16, CD23, CD25, CD32 and CD64 Ag or HLA-DR were observed between PBM from the different patient groups or healthy controls. Taken together, this study shows that long-term local therapy with inhaled BDP coincides with an altered expression of at least one cell surface Ag on PBM from allergic asthmatics.

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The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

10.21203/rs.2.20386/v2 ◽

2020 ◽

Author(s):

Roman Wójcik ◽

Joanna Małaczewska ◽

Grzegorz Zwierzchowski ◽

Jan Miciński ◽

Edyta Kaczorek-Łukowska

Keyword(s):

Oxidative Metabolism ◽

Respiratory Burst ◽

Peripheral Blood ◽

Phagocytic Activity ◽

Chemotactic Activity ◽

Control Group ◽

Cell Mediated Immunity ◽

Specific Cell ◽

Group I ◽

Experimental Group

Abstract Background The objective of this study was to evaluate the effect of HMB on the chemotactic activity, phagocytic activity and respiratory burst of peripheral blood granulocytes and monocytes in calves. Method The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB (Metabolic Technologies Inc. Ames, IA, USA) at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine: chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and respiratory burst (BURSTTEST® kit) of peripheral blood granulocytes and monocytes by flow cytometry. Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). HMB also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate), expressed in terms of the percentage of oxidative metabolism and MFI. Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. The supplementation of animal diets with HMB for both preventive and therapeutic purposes can also reduce the use of antibiotics in animal production.

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Respiratory burst in alveolar macrophages of diabetic rats

Journal of Applied Physiology ◽

10.1152/jappl.1990.68.6.2384 ◽

1990 ◽

Vol 68 (6) ◽

pp. 2384-2390 ◽

Cited By ~ 20

Author(s):

V. Mohsenin ◽

J. Latifpour

Keyword(s):

Alveolar Macrophages ◽

Respiratory Burst ◽

Peripheral Blood ◽

Diabetic Rats ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Significant Difference ◽

Cytochrome C Reduction ◽

Generating Capacity ◽

O2 Production

Bactericidal ability of alveolar macrophages is depressed in rats with diabetes mellitus. To define the mechanism of this abnormality, we measured the parameters of respiratory burst in alveolar macrophages, peripheral blood monocytes, and neutrophils of rats 8 wk after the induction of diabetes by streptozocin. Superoxide anion (O2-.) generation during basal conditions and after stimulation with phorbol myristate acetate (PMA) was measured as superoxide dismutase-inhibitable cytochrome c reduction. NADPH, the principal substrate for NADPH-oxidase-dependent O2-. generation, was measured in the alveolar macrophages and quick-frozen lungs by the enzyme-cycling method. O2-. generation after PMA was significantly lower in the alveolar macrophages of diabetics than in the controls (14.4 +/- 2.0 nmol.10(6) cells-1.20 min-1 vs. 26.2 +/- 1.9, P less than 0.05). Conversely the peripheral blood monocytes of diabetics demonstrated an enhanced O2-. production after PMA stimulation. There was no significant difference in the neutrophil O2-.-generation between the groups. The alveolar macrophage NADPH (control 0.44 +/- 0.15 nmol/10(6) cells vs. diabetic 0.21 +/- 0.04, P less than 0.05) and lung tissue NADPH levels (control 81.4 +/- 16.3 nmol/g dry wt vs. diabetic 35.8 +/- 20.5, P less than 0.05) were significantly lower in the diabetics than in the controls. These data indicate that the O2-.-generating capacity of alveolar macrophages is markedly depressed in diabetes, whereas their precursors, monocytes, are primed to generate O2-. with PMA stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)

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Controlled clinical trial of interferon-gamma as postoperative surgical adjuvant therapy for colon cancer.

Journal of Clinical Oncology ◽

10.1200/jco.1995.13.9.2324 ◽

1995 ◽

Vol 13 (9) ◽

pp. 2324-2329 ◽

Cited By ~ 41

Author(s):

M Wiesenfeld ◽

M J O'Connell ◽

H S Wieand ◽

N J Gonchoroff ◽

J H Donohue ◽

...

Keyword(s):

Colon Cancer ◽

High Risk ◽

Immune Function ◽

Interferon Gamma ◽

Peripheral Blood ◽

Significant Enhancement ◽

Blood Monocytes ◽

Ifn Gamma ◽

Peripheral Blood Monocytes ◽

Hla Dr

PURPOSE The primary goal of this study was to assess the effectiveness of interferon gamma (IFN-gamma) to prevent tumor relapse following potentially curative surgery in patients with high-risk colon cancer. A secondary goal was to determine the effect of IFN-gamma on immune function and to correlate alterations in immune parameters with survival. PATIENTS AND METHODS Three to 4 weeks after undergoing resection of all known malignant disease, 99 patients with stage II, III, or IV colon cancer were randomly assigned to receive IFN-gamma 0.2 mg total dose by subcutaneous injection daily for 6 months or observation. Serial assessment of human leukocyte antigen (HLA)-DR expression and Fc receptors on peripheral-blood monocytes was conducted in 24 patients who received IFN-gamma and 27 control patients. RESULTS With a median follow-up duration of 59 months in patients still alive, there was evidence of a detrimental effect on time to relapse (P = .03) among patients who received IFN-gamma. There was no significant difference in patient survival (P = .12). This study has sufficient power to rule out a 25% reduction in death rate for patients who received IFN-gamma (P < .05). Significant enhancement of immune function was observed in patients treated with IFN-gamma as measured by HLA-DR expression (P < .01) and Fc receptors (P < .001) on peripheral-blood monocytes. CONCLUSION This study effectively rules out any clinically meaningful benefit for IFN-gamma as surgical adjuvant treatment for patients with high-risk colon cancer. Although significant enhancement of nonspecific immune function was seen with this dosage administration schedule of IFN-gamma, this was not associated with any demonstrable antitumor effect.

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Biochemical basis of HLA-DR and CR3 modulation on human peripheral blood monocytes by lipopolysaccharide

Cellular Immunology ◽

10.1016/0008-8749(87)90209-7 ◽

1987 ◽

Vol 108 (1) ◽

pp. 242-248 ◽

Cited By ~ 12

Author(s):

Kenneth R. McLeish ◽

Samuel R. Wellhausen ◽

William L. Dean

Keyword(s):

Peripheral Blood ◽

Human Peripheral Blood ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Biochemical Basis ◽

Hla Dr

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The Early Expression of HLA-DR and CD64 Myeloid Markers Is Specifically Compartmentalized in the Blood and Lungs of Patients with Septic Shock

Mediators of Inflammation ◽

10.1155/2016/3074902 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Tomasz Skirecki ◽

Małgorzata Mikaszewska-Sokolewicz ◽

Grażyna Hoser ◽

Urszula Zielińska-Borkowska

Keyword(s):

Septic Shock ◽

Peripheral Blood ◽

Prognostic Value ◽

Lavage Fluid ◽

Blood Monocytes ◽

Activation Markers ◽

Circulating Cells ◽

Neutrophil Cd64 ◽

Hla Dr ◽

The Status

Identification of reliable biomarkers is key to guide targeted therapies in septic patients. Expression monitoring of monocyte HLA-DR and neutrophil CD64 could fulfill the above need. However, it is unknown whether their expression on circulating cells reflects the status of tissue resident cells. We compared expressions of HLA-DR and CD64 markers in the circulation and airways of septic shock patients and evaluated their outcome prognostic value. The expression of CD64 on neutrophils and HLA-DR on monocytes was analyzed in the peripheral blood and mini-bronchoalveolar lavage fluid cells by flow cytometry. Twenty-seven patients with septic shock were enrolled into the study. The fluorescence intensity of HLA-DR on circulating monocytes was 3.5-fold lower than on the pulmonary monocytes (p=0.01). The expression of CD64 on circulating and airway neutrophils was similar (p=0.47). Only the expression of CD64 on circulating neutrophils was higher in nonsurvivors versus survivors (2.8-fold;p=0.031). Pulmonary monocytes display a higher level of HLA-DR activation compared to peripheral blood monocytes but the expression of neutrophil CD64 is similar on lung and circulating cells. Death in septic patients was effectively predicted by neutrophil CD64 but not monocytic HLA-DR. Prognostic value of cellular activation markers in septic shock appears to strongly depend on their level of compartmentalization.

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