SYNCHRONIZING THE in vitro GERMINATION OF Psidium guineense Sw. SEEDS BY MEANS OF OSMOTIC PRIMING

ABSTRACT The Brazilian guava (Psidium guineense Swartz) is seed-propagated and, being native to the Caatinga biome, may frequently have uneven germination.Thus, we aimed to evaluate the synchronization of the in vitro seed germination of three accessions of the Brazilian guava, using water, polyethyleneglycol (PEG 6000), and potassium nitrate (KNO3) at different potentials and times of osmotic priming. Seeds from three accessions of the Brazilian guava (Y85, Y93,and Y97) from the UNEB/BA Germplasm Active Bank were subjected to the following pretreatments: -0.6, -1.0, -1.4, and -1,8 MPa PEG 6000; 10 and 20% KNO3 for 24h; 10 and 20% KNO3 for 48h; water for 24 and 48h; and non-primed seeds as the control. The experimental design was therefore a 10x3+1 factorial scheme. We assessed the germination percentage (G), mean germination time (MGT), germination speed (GS), and germination speed index (GSI). Data was subjected to analysis of variance followed by a means test (Duncan at 5% probability) and regression. There was interaction between the priming treatments and accessions for all evaluated features, except G. PEG 6000 decreased the MGT (from 6 to 8 days) and increased GS and GSI of seeds from all three accessions at potentials -1.0 to -1.5 MPa.Water-priming had a positive effect on MGT, GS, and GSI of accession Y85 seeds. KNO3 negatively affected germination of seeds from all three accessions. Thereby, we could synchronize seed germination of accessions Y85 and Y97 with PEG 6000.

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Interruption of Seed Dormancy and In Vitro Germination of the Halophile Emerging Crop Suaeda edulis (Chenopodiaceae)

Agronomy ◽

10.3390/agronomy12010103 ◽

2021 ◽

Vol 12 (1) ◽

pp. 103

Author(s):

Francyelli Regina Costa-Becheleni ◽

Enrique Troyo-Diéguez ◽

Alejandra Nieto-Garibay ◽

Luis Alejandro Bustamante-Salazar ◽

Hugo Sergio García-Galindo ◽

...

Keyword(s):

Salinity Gradient ◽

Germination Rate ◽

Germination Percentage ◽

In Vitro Germination ◽

Best Response ◽

Best Value ◽

Germination Speed ◽

Mean Germination Time ◽

The Mean

Suaeda edulis (Flores Olvera & Noguez) is a halophile species that grows in saline environments, with concomitant difficulties to germinate and reproduce. Once a null germination under a salinity gradient or fresh water was observed, a plant-growth bioregulator (BioR) was applied to interrupt dormancy and improve germination in vitro. This BioR is composed of gibberellins and adjuvant regulators; and 12.5, 25.0, and 37.5 mg L−1 of gibberellins with immersion at 24, 48, and 72 h were assayed. Most of the normality and homoscedasticity tests were favorable, except in three of 24 cases. On the germination percentage (transformed values), the dose 25.0 mg L−1 reflected the highest values of 5.5 and 6.0 units at 48 and 72 h. For the mean germination time, the highest level of 37.5 mg L−1 at 24 h reflected the best response. For the germination rate, the best one was 25 mg L−1 at 48 h, reaching 12% per day, but for the germination speed coefficient, the best value was observed with 25.0 mg L−1 at 24 h. It was concluded that to improve germination of S. edulis seeds, it is necessary to assess a dose-gradient of gibberellins, starting at 25.0 mg L−1, with higher values to break dormancy.

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The influence of light spectra, UV-A, and growth regulators on the in vitro seed germination of Senecio cineraria DC.

Revista CERES ◽

10.1590/s0034-737x2010000500002 ◽

2010 ◽

Vol 57 (5) ◽

pp. 576-580 ◽

Cited By ~ 1

Author(s):

Cristiane Pimentel Victório ◽

Nina Cláudia Barbosa da Silva ◽

Maria Apparecida Esquibel ◽

Alice Sato

Keyword(s):

Seed Germination ◽

Growth Regulators ◽

White Light ◽

Germination Rate ◽

Germination Percentage ◽

Ms Medium ◽

In Vitro Germination ◽

Light Spectra ◽

Indole 3 Acetic Acid

This study was carried out to investigate the effects of light spectra, additional UV-A, and different growth regulators on the in vitro germination of Senecio cineraria DC. Seeds were surface-sterilized and inoculated in MS medium to evaluate the following light spectra: white, white plus UV-A, blue, green, red or darkness. The maximum germinability was obtained using MS0 medium under white light (30%) and MS + 0.3 mg L-1 GA3 in the absence of light (30.5%). S. cineraria seeds were indifferent to light. Blue and green lights inhibited germination. Different concentrations of gibberellic acid (GA3) (0.1; 0.4; 0.6; 0.8; 1.0 and 2.0 mg L-1) and indole-3-acetic acid IAA (0.1; 0.3 and 1.0 mg L-1) were evaluated under white light and darkness. No concentration of GA3 enhanced seed germination percentage under white light. However, when the seeds were maintained in darkness, GA3 improved germination responses in all tested concentrations, except at 1.0 mg L-1. Under white light, these concentrations also increased the germination time and reduced germination rate. Germination rate, under light or darkness, was lower using IAA compared with GA3.

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Influence of priming on Eucalyptus spp seeds' tolerance to salt stress

Journal of Seed Science ◽

10.1590/2317-1545v38n4165060 ◽

2016 ◽

Vol 38 (4) ◽

pp. 329-334 ◽

Cited By ~ 4

Author(s):

Anderson Cleiton José ◽

Natália Cristina Nogueira Silva ◽

José Marcio Rocha Faria ◽

Wilson Vicente Souza Pereira

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Water Potential ◽

Salt Solution ◽

Germination Percentage ◽

Eucalyptus Urophylla ◽

Nacl Solution ◽

Peg 6000 ◽

Germination Speed ◽

Osmotic Potentials

Abstract: The objective of this experiment was to evaluate the effect of priming on the germination of Eucalyptus urophylla and of hybrid E. urophylla × E. grandis seeds under salt stress. Two osmotic potentials (-1.0 and -1.5 MPa) were tested, using PEG 6000 for 1 and 3 days. After priming, seeds were germinated under salt stress in a NaCl solution at 0.0 (control), -0.5, -0.75 and -1.0 MPa potentials, at 25 °C. Seed germination and germination speed index decreased as the water potential of the germination medium decreased. However, E. urophylla was more tolerant to salt stress; it showed a higher germination percentage under all tested potentials, when compared to the hybrid. The osmotic conditioning at -1.0 MPa for three days was more effective when E. urophylla x E. grandis was germinated in a salt solution at -1.0 MPa, indicating that this treatment was more effective in inducing tolerance to salt stress.

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Southern highland papaya (Vasconcellea quercifolia A. St.-Hil.): Do fruit ripening and harvesting time affect seed germination?

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v42i1.42825 ◽

2019 ◽

Vol 42 ◽

pp. e42825 ◽

Cited By ~ 1

Author(s):

María Manuela Urtasun ◽

Eugenia Mabel Giamminola ◽

Marta Leonor de Viana

Keyword(s):

Seed Germination ◽

Fruit Ripening ◽

Storage Period ◽

Seed Storage ◽

Potassium Nitrate ◽

Germination Percentage ◽

Harvesting Time ◽

Germination Time ◽

Light Requirements ◽

Mean Germination Time

In this work, we report the effects of the harvesting time, the stages in fruit ripening and the influence of potassium nitrate in V. quercifolia seed germination. In addition, information about the storage period and light requirements is provided. Fruits were harvested at the beginning and at the end of the fruiting season, and they were classified into five ripening categories. Seed germination was evaluated with two factorial experiments: 1) harvesting time, fruit ripening, and pre-germination treatment; 2) storage and light requirements. The response variables were germination percentage, mean germination time, and seedling vigor. Seeds harvested at the beginning of the season appeared to be less dormant and they were not influenced by fruit ripening or pre-germination treatments. By contrast, seeds harvested at the end of the season were influenced by fruit ripening and pre-germination treatments. Light and seed storage had a positive effect on germination. Mean germination time varied from 12 to 40 days, and vigor index was positively influenced by potassium nitrate. V.quercifolia seeds are photoblastic positive at constant temperatures and their dormancy can be influenced by harvest time, fruit ripening and a storage period.

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AUTOTOXICITY IN TOUGH LOVEGRASS ( Eragrostis plana )

Planta Daninha ◽

10.1590/s0100-83582017350100037 ◽

2017 ◽

Vol 35 (0) ◽

Author(s):

A. FAVARETTO ◽

S.M. SCHEFFER-BASSO ◽

N.B. PEREZ

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Germination Percentage ◽

Shoot Length ◽

Aqueous Extracts ◽

Early Seedling Growth ◽

Germination Speed ◽

Early Seedling ◽

Mean Germination Time ◽

Growth Of Seedlings

ABSTRACT Autotoxicity in tough lovegrass has been analyzed in the field, but has never been tested in allelopathy bioassays. Therefore, this study aimed to verify and compare the autotoxicity of leaves and roots from aqueous extracts of tough lovegrass on its germination and early seedling growth. Extracts of green leaves (GL), senescent leaves (SL), and roots (R) at concentrations of 0.05, 0.15, and 0.25 g mL-1 were analyzed on how they affected seed germination and seedling growth. The extracts, regardless of the source or concentration of the material used, have significantly reduced germination percentage, index of germination speed, and root growth of seedlings of tough lovegrass. Effects on mean germination time and shoot length varied according to the origin and concentration of the extracts. GL, at concentrations 0.15 and 0.25 g mL-1, showed the most damaging effect. In tough lovegrass, autotoxicity is a mechanism that may be involved in the control of germination and plant growth, which could explain the distance between their clumps in the field.

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In vitro germination to overcome dormancy in seeds of ‘Red Globe’, ‘Italia’ and ‘Niagara Rosada’ grapes

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452019495 ◽

2019 ◽

Vol 41 (5) ◽

Author(s):

Andressa Leal Generoso ◽

Alexandre Pio Viana ◽

Virginia Silva Carvalho ◽

Otalício Damásio da Costa Júnior

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Growth Media ◽

Seedling Vigor ◽

In Vitro Germination ◽

Grape Seeds ◽

Breeding Programs ◽

Promising Alternative ◽

Niagara Rosada

Abstract Dormancy in grape seeds slows the progress of many breeding programs of the crop, culminating in low uniformity and low germination percentages. Conventional methods used to overcome dormancy are time-consuming. Thus, in vitro seed germination emerges as a promising alternative to ensure the germination of grape seeds. In this study, we examined the in vitro germination and vigor of seedlings originating from seeds of ‘Red Globe’, ‘Italia’ and ‘Niagara Rosada’ grapes in growth media supplemented with five concentrations of gibberellic acid (GA3) (0, 1.41, 2.83, 4.24 and 5.66 µmol L-1) for 47 days. The use of GA3 increased seed germination percentage and seedling vigor, in the three varieties. Therefore, for the in vitro germination of seeds of ‘Red Globe’ can be used between 1.41 to 4.24 µmol L-1 of GA3. For ‘Italia’ grapes is indicated 1.41 µmol L-1 from GA3 and for ‘Niagara Rosada’ grape is as between 1.41 to 5.66 µmol L-1 from GA3 can be used.

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Germination and salinity tolerance of seeds of sixteen Fabaceae species in Thailand for reclamation of salt-affected lands

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210547 ◽

2020 ◽

Vol 21 (5) ◽

Author(s):

Yongkriat KU-OR ◽

Nisa Leksungnoen ◽

Damrongvudhi Onwimon ◽

Peerapat Doomnil

Keyword(s):

Seed Germination ◽

Salinity Tolerance ◽

Germination Percentage ◽

Percentage Germination ◽

Salt Tolerant ◽

Senna Siamea ◽

Sesbania Grandiflora ◽

Germination Speed ◽

Salinity Levels ◽

Mean Germination Time

Abstract. Ku-Or Y, Leksungnoen N, Onwinom D, Doomnil P. 2020. Germination and salinity tolerance of seeds of sixteen Fabaceae species in Thailand for reclamation of salt-affected lands. Biodiversitas 21: 2188-2200. Over the years, areas affected by salinity have increased dramatically in Thailand, resulting in an urgent need for reclamation of salt-affected areas using salinity tolerant plant species. In this context, seed germination is an important process in plant reproduction and dispersion. This research aimed to study the ability of 16 fabaceous species to germinate and tolerate salt concentrations of at 6 different levels (concentration of sodium chloride solution, i.e., 0, 8, 16, 24, 32, and 40 dS m-1). The germination test was conducted daily for 30 days, and parameters such as germination percentage, germination speed, and germination synchrony were calculated. The electrical conductivity (EC50) was used to compare the salt-tolerant ability among the 16 species. Our results showed that the germination percentage, germination speed, and germination synchrony of all species decreased with an increase in salinity concentration. The mean germination time increased from 2-4 days under 0 dS m-1 to 4-10 days under 16 dS m-1, indicating that the seeds germinated at a slower rate as the salinity levels increased. Seed germination percentage was more than 80% across all the species at salinity levels between 0 to 16 dS m-1. Sesbania grandiflora, Senna siamea, and Dalbergia cochinchinensis had a high EC50 value of 33.56, 32.93, and 30.83 dS m-1 respectively, suggesting that these species were the three most salt-tolerant species in this study. As such, the establishment of these species in salt-affected areas should be studied further in order to reclaim such affected lands.

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Chitosan coating on bean and maize seeds: release of agrochemical fungicide and post-storage condition

Journal of Seed Science ◽

10.1590/2317-1545v43254286 ◽

2021 ◽

Vol 43 ◽

Author(s):

Nancy Araceli Godínez-Garrido ◽

Juan Gabriel Ramírez-Pimentel ◽

Jorge Covarrubias-Prieto ◽

Francisco Cervantes-Ortiz ◽

Artemio Pérez-López ◽

...

Keyword(s):

Seed Germination ◽

Defense Mechanisms ◽

Germination Rate ◽

Storage Condition ◽

Germination Percentage ◽

Negative Effects ◽

Retention Capacity ◽

Chitosan Coating ◽

Maize Seeds ◽

Germination Speed

Abstract: Chitosan is a biopolymer obtained from deacetylation of chitin; it has multiple applications in agriculture as an antifungal, soil conditioner, inducer of defense mechanisms, fruits postharvest coating, leaves and seeds, among others. The objective in this research was to evaluate the effect of chitosan coatings mixed with fungicide (dithiocarbamate) on the germination and germination speed of bean and maize seeds in storage and to determine the retention capacity of the fungicide in the coated seeds under different times of imbibition. Two coating treatments at concentrations of 0.1 and 0.5% chitosan in water, two coatings treatments at 0.1 and 0.5% chitosan supplemented with 0.5% fungicide and a coating without chitosan using only 0.5% fungicide in water were used in bean and maize seed; and as control seeds imbibed in distilled water were used; after treatments, germination percentage and germination speed were determined, also fungicide release were determined at 0, 1, 2 and 6 h of imbibition, and the effect of storage time on germination and germination speed was determined at 30, 60, 90, 120, 150 and 180 days of storage at 4 °C and 45% relative humidity. The fungicide release effect was determined by inhibiting Fusarium oxysporum conidia germination. There were no negative effects of coatings on seed germination after storage. The treatment that provided both greater retention of the fungicidal agent and released it gradually, was 0.5% chitosan mixed with fungicide concentration. Chitosan coating seeds mixed with fungicide do not cause negative changes in seed germination or germination rate.

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Control of browning during seed germination of Meizotropis pellita: a rare and an endangered plant

Research Journal of Biotechnology ◽

10.25303/168rjbt11821 ◽

2021 ◽

Vol 16 (8) ◽

pp. 118-120

Author(s):

Renu Chandola ◽

Anshulika Upadhyaya

Keyword(s):

Seed Germination ◽

Culture Media ◽

Light Condition ◽

Endangered Plant ◽

In Vitro Germination ◽

Light Conditions ◽

Low Intensity ◽

Browning Reaction ◽

Threatened Plant Species

Meizotropis pellita is a rare, endangered and threatened plant species found in Patwadanger forest. In nature this species grows from rootstock in precise and sensitive habitats. However, phenolic compounds provoke browning reaction which is responsible for lethal browning in plant tissue culture and hindering in vitro germination. The aim of this study was to minimize the effect of phenolic compound during seed germination. We evaluate different antibrowning treatments in Meizotropis pellita seeds. The seeds were pretreated in Polyvinylpyrrolidone and then treated seeds were cultured on MS media and sterilized seeds were directly inoculated on PVP containing MS media. These culture media incubate under dark and light conditions at different durations respectively. Our results showed that addition of antibrowning agent, PVP inhibit onset of browning. Dark and light condition also played a crucial role on onset of browning. Delayed and low intensity of browning were observed in case of seeds that were inoculated on MS media containing PVP and were kept in dark. The present research offers a positive in vitro seed germination protocol for Meizotropis pellita conservation.

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Can Bulk and Nanosized Titanium Dioxide Particles Improve Seed Germination Features of Wheatgrass (Agropyron desertorum)

Notulae Scientia Biologicae ◽

10.15835/nsb539072 ◽

2013 ◽

Vol 5 (3) ◽

pp. 325-331 ◽

Cited By ~ 13

Author(s):

Reyhaneh AZIMI ◽

Hassan FEIZI ◽

Mohammad KHAJEH HOSSEINI

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Germination Time ◽

Positive Effects ◽

Tio2 Particles ◽

Nanosized Titanium Dioxide ◽

Mean Germination Time ◽

Germination Value ◽

Improve Seed Germination ◽

Nanosized Tio2

The goal of this study was to evaluate concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L-1 with bulk TiO2 for possible stimulatory effects on wheatgrass seed germination and early growth stage. After 14 days of seed incubation, germination percentage improved by 9% following exposure to 5 ppm nanosized TiO2 treatment comparing to control. Similar positive effects occurred in terms of germination value and mean daily germination. Application of bulk TiO2 particles in 80 ppm concentration greatly decreased the majority of studied traits. Therefore phytotoxicity effect observed on wheatgrass seedling by application of bulk TiO2 particles in 80 ppm concentration. Exposure of wheatgrass seeds to 5 ppm nanosized TiO2 and bulk and nanosized TiO2 at 60 ppm obtained the lowest mean germination time but higher concentrations did not improve mean germination time. In general, there was a positive response by wheatgrass seed to some concentrations of nanosized TiO2. Usage of nanoparicles in order to improve germination and establishment of range plant in adverse environments similar to rangeland could be possible.

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