scholarly journals Germination and Dormancy in Seeds of Sorghum halepense and Sorghum arundinaceum

2015 ◽  
Vol 33 (2) ◽  
pp. 223-230 ◽  
Author(s):  
F.H. Krenchinski ◽  
A.J.P. Albrecht ◽  
L.P. Albrecht ◽  
H.L. Villetti ◽  
G. Orso ◽  
...  
Keyword(s):  
Germination Percentage ◽  
The Other ◽  
Sorghum Halepense ◽  
Design Experiment ◽  
Dormancy Breaking ◽  
Randomized Design ◽  
Breaking Dormancy ◽  
Completely Randomized Design ◽  
Presence And Absence ◽  
Double Factorial

Light, temperature and dormancy are factors that influence the germination of seeds and are strictly linked to the emergence of weeds. The objective of this work was to assess the germination of Sorghum arundinaceum and Sorghum halepense subjected to different conditions of temperature and luminosity, as well as assessing seed dormancy breaking mechanisms. For this, two experiments were conducted, both arranged in a completely randomized design. Experiment 1 was installed in a 2 x 5 double factorial design. The first factor was the absence or presence of light for 12 hours, and the other was composed of five constant temperatures: 15, 20, 30, 40 and 45 oC. In experiment 2, the efficiency of nine treatments used for breaking dormancy of seeds was assessed. The variables analyzed for both experiments were germination percentage and germination speed index (GSI). For the statistical analysis were performed an analysis of variance (ANOVA) and all the necessary consequences, as well as regression, when relevant. In experiment 1 for both species greater germination occurred in the presence of light. For S. arundinaceum the temperatures at which there was the highest percentage of germination were 33.13 and 31.24 oC for presence and absence of light respectively. As for S. halepense these temperatures were 31.98 and 29.75 oC for presence and absence of light respectively. As for the treatments for breaking dormancy, the mechanical scarification of seeds with sandpaper presented the highest germination and GSI. It is concluded that the Sorghum species studied are neutral photoblastic seeds and present mechanical type dormancy.

scholarly journals Métodos para a Superação de Dormência da Tarumarana

UNICIÊNCIAS ◽  
2021 ◽  
Vol 24 (2) ◽  
pp. 135-140
Author(s):  
Cristiane Ramos Vieira ◽  
Joás Dos Santos Soares ◽  
Bruno Conceição de Veiga ◽  
Maicon Marinho Vieira Araujo ◽  
Rayza Samara de Assis Carneiro
Keyword(s):  
Sulfuric Acid ◽  
Germination Percentage ◽  
Shoot Length ◽  
Hot Water ◽  
Initial Growth ◽  
Forest Species ◽  
Randomized Design ◽  
Breaking Dormancy ◽  
Germination Speed ◽  
Completely Randomized Design

A tarumarana (Buchenavia tomentosa Eichler) é uma espécie florestal com potencial para a recomposição florestal no bioma Cerrado. No entanto, a formação das mudas dessa espécie, via semente, fica comprometida, por esta apresentar dormência tegumentar. Com base nessa problemática, desenvolveu-se um experimento com o objetivo de avaliar a escarificação ácida e a imersão em água, como métodos para a superação da dormência de pirênios de tarumarana. Os tratamentos testados foram: T0 – sem tratamento para superação da dormência; T1 – imersão em água quente por uma hora; T2 – imersão em água quente por uma hora e meia; T3 – imersão em água quente por duas horas; T4 – imersão em ácido sulfúrico por uma hora; T5 – imersão em ácido sulfúrico por uma hora e meia. O experimento foi realizado em delineamento inteiramente casualizado, com 20 pirênios de tarumarana por repetição. Ao final de 30 dias, a contar do começo das emergências, foram avaliadas as seguintes variáveis: índice de velocidade de germinação (IVG), o tempo médio de germinação (TMG), porcentagem de germinação (G) e o índice de velocidade de germinação (IVG), comprimento da parte aérea (H) e diâmetro de coleto (DC). O método mais eficiente para a superação da dormência dos pirênios de tarumarana foi a imersão em água quente por uma hora e meia, que garantiu a maior taxa de germinação. No entanto, a imersão em água quente, como método para superação da dormência de pirênios de tarumarana, não influenciou de forma significativa no crescimento inicial em altura e em diâmetro das mudas obtidas.   Palavras-chave: Buchenavia Tomentosa. Ácido Sulfúrico. Água Quente. Quebra de Dormência. Semente Florestal.   Abstract Tarumarana (Buchenavia tomentosa Eichler) is a forest species with potential for restoration in the Cerrado biome. However, the formation of seedlings of this species, via seed, is compromised, because it presents integumentary dormancy. Based on this problem, an experiment was developed with the objective of evaluating acid scarification and immersion in water, as methods for overcoming dormancy in tarumarana pyrenes. The tested treatments were: T0 - without treatment to overcome dormancy; T1 - immersion in hot water for one hour; T2 - immersion in hot water for one hour and a half; T3 - immersion in hot water for two hours; T4 - immersion in sulfuric acid for one hour; T5 - immersion in sulfuric acid for one hour and a half. The experiment was carried out in completely randomized design, with 20 pyrenes of tarumarana per repetition. At the end of 30 days, counting from the beginning of emergencies, the following variables were evaluated: germination speed index (IVG), average germination time (TMG), germination percentage (G), the germination speed index (IVG), shoot length (H) and diameter (DC). The most efficient method for overcoming dormancy in tarumarana pyrenes was immersion in hot water for one hour and a half, which guaranteed the highest germination percentage. However, immersion in hot water, as a method to overcome dormancy of tarumarana pyrenes, did not influence, significantly, the initial growth in height and diameter of the seedlings obtained.   Keywords: Buchenavia tomentosa. Sulfuric Acid. Hot Water. Breaking Dormancy. Forest Seed.

scholarly journals Isolation and Characterization of Yeasts from Rumen Fluids for Potential Use as Additives in Ruminant Feeding

2021 ◽  
Vol 8 (3) ◽  
pp. 52
Author(s):  
Chanon Suntara ◽  
Anusorn Cherdthong ◽  
Metha Wanapat ◽  
Suthipong Uriyapongson ◽  
Vichai Leelavatcharamas ◽  
...  
Keyword(s):  
Incubation Time ◽  
Cellulase Activity ◽  
The Other ◽  
Pichia Kudriavzevii ◽  
Factor B ◽  
Yeast Strains ◽  
Randomized Design ◽  
Holstein Friesian ◽  
The Media ◽  
Completely Randomized Design

Saccharomyces cerevisiae is a yeast strain often used to improve the feed quality of ruminants. However, S. cerevisiae has limited capacity to provide biomass when inoculated with carbon sources and a low ability to produce cellulase enzymes. Here, we hypothesized that yeast in the rumen produces a large amount of biomass and could release cellulase enzymes to break down fiber content. Therefore, the aim of this study was to screen, isolate and identify yeast from the rumen fluids of Holstein Friesian steers and measure the efficiency of biomass production and cellulase activity. A fermentation medium containing sugarcane molasses as a carbon source and urea as a nitrogen source was optimized. Two fistulated–crossbred Holstein Friesian steers averaging 350 ± 20 kg body weight were used to screen and isolate the ruminal yeast. Two experiments were designed: First, a 12 × 3 × 3 factorial was used in a completely randomized design to determine biomass and carboxymethyl cellulase activity. Factor A was the isolated yeast and S. cerevisiae. Factor B was sugarcane molasses (M) concentration. Factor C was urea (U) concentration. In the second experiment, potential yeasts were selected, identified, and analyzed for 7 × 4 factorial use in a completely randomized design. Factor A was the incubation times. Factor B was the isolated yeast strains, including codes H-Khon Kaen University (KKU) 20 (as P. kudriavzevii-KKU20), I-KKU20 (C. tropicalis-KKU20), and C-KKU20 (as Galactomyces sp.-KKU20). Isolation was imposed under aerobic conditions, resulting in a total of 11 different colonies. Two appearances of colonies including asymmetric colonies of isolated yeast (indicated as A, B, C, E, and J) and ovoid colonies (coded as D, F, G, H, I, and K) were noted. Isolated yeast from the rumen capable of providing a high amount of biomass when inoculant consisted of the molasses 15% + urea 3% (M15 + U3), molasses 25% + urea 1% (M25 + U1), molasses 25% + urea 3% (M25 + U3), and molasses 25% + urea 5% (M25 + U5) when compared to the other media solution (p < 0.01). In addition, 11 isolated biomass-producing yeasts were found in the media solution of M25 + U1. There were 4 isolates cellulase producing yeasts discovered in the media solution of M25 + U1 and M25 + U5 whereas molasses 5% + urea 1% (M5 + U1), molasses 5% + urea 3% (M5 + U3), molasses 5% + urea 5% (M5 + U5), molasses 15% + urea 1% (M15 + U1), molasses 15% + urea 3% (M5 + U3), and M25 + U3 were found with 2, 3, 1, 2, 1, and 2 isolates, respectively. Ruminal yeast strains H-KKU20, I-KKU20, and C-KKU20 were selected for their ability to produce biomass. Identification of isolates H-KKU20 and I-KKU20 revealed that those isolates belonged to Pichia kudriavzevii-KKU20 and Candida tropicalis-KKU20 while C-KKU20 was identified as Galactomyces sp.-KKU20. Two strains provided maximum cell growth: P. kudriavzevii-KKU20 (9.78 and 10.02 Log cell/mL) and C. tropicalis-KKU20 (9.53 and 9.6 Log cells/mL) at 60 and 72 h of incubation time, respectively. The highest ethanol production was observed in S. cerevisiae at 76.4, 77.8, 78.5, and 78.6 g/L at 36, 48, 60, and 72 h of incubation time, respectively (p < 0.01). The P. kudriavzevii-KKU20 yielded the least reducing sugar at about 30.6 and 29.8 g/L at 60 and 72 h of incubation time, respectively. The screening and isolation of yeasts from rumen fluids resulted in 11 different yeasts being obtained. The potential yeasts discovered in the rumen fluid of cattle were Pichia kudriavzevii-KKU20, Candida tropicalis-KKU20, and Galactomyces sp.-KKU20. P. kudriavzevii-KKU20 had higher results than the other yeasts in terms of biomass production, cellulase enzyme activity, and cell number.

scholarly journals Infestation of froghopper nymphs changes the amounts of total phenolics in sugarcane

2005 ◽  
Vol 62 (6) ◽  
pp. 543-546 ◽  
Author(s):  
Rafael José Navas da Silva ◽  
Eduardo Rossini Guimarães ◽  
José Francisco Garcia ◽  
Paulo Sérgio Machado Botelho ◽  
Maria Inês Tiraboschi Ferro ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Low Temperature ◽  
Temperature Variation ◽  
Total Phenolics ◽  
The Other ◽  
Plant Phenolics ◽  
High Moisture ◽  
Randomized Design ◽  
Leaf Tissues ◽  
Completely Randomized Design

The increased rate of sugarcane harvest without previous burn has provided a very favorable environment to the froghopper Mahanarva fimbriolata (Stal, 1854), with high moisture and low temperature variation. Few works have studied the response of sugarcane to this pest, so little is known about resistant cultivars. Plant phenolics are widely studied compounds because of their known antiherbivore effect. This research aims to determine if the attack of M. fimbriolata nymphs stimulates the accumulation of total phenolics in sugarcane. The experiment was carried out in greenhouse and arranged in completely randomized design, in a 3 X 2 X 4 factorial with three replications. Second instar nymphs of M. fimbriolata were infested at the following rates: control, 2-4 and 4-8 nymphs per pot (first-second infestations, respectively). Pots were covered with nylon net and monitored daily to isolate the effect of leaf sucking adults. Leaf and root samples were collected and kept frozen in liquid nitrogen until analyses. Infested plants showed higher levels of phenolics in both root and leaf tissues. In roots, the cultivar SP80-1816 accumulated more phenolic compounds in response to the infestation of M. fimbriolata. On the other hand, higher levels were found in leaves and roots of control plants of SP86-42, which might be an indication of a non-preference mechanism. The increase of total phenolics in sugarcane infested with root-sucking froghopper nymphs does not seem to be useful to detect the resistance to this pest.

scholarly journals Size and vigor of Anadenanthera colubrina (Vell.) Brenan seeds harvested in Caatinga areas

2017 ◽  
Vol 39 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Jaciara de Souza Bispo ◽  
Danielle Carolina Campos da Costa ◽  
Samara Elizabeth Vieira Gomes ◽  
Gilmara Moreira de Oliveira ◽  
Janete Rodrigues Matias ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Dry Matter ◽  
Folk Medicine ◽  
Germination Percentage ◽  
Climate Variations ◽  
Randomized Design ◽  
Mother Plants ◽  
Physiological Quality ◽  
Completely Randomized Design

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.

scholarly journals Analisis Efektifitas Tepung Jamur sebagai Larvasida Aedes aegypti

2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam
Keyword(s):  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Storage Time ◽  
Natural Sciences ◽  
The Other ◽  
Biology Department ◽  
Randomized Design ◽  
Two Factors ◽  
Result Show ◽  
Completely Randomized Design

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.

scholarly journals Response of Quality Attributes of Banana (Musa paradisiaca L.) cv. Williumto Post Shooting Spray and Bunch Covering Material

2021 ◽  
pp. 38-45
Author(s):  
D. R. Paradva ◽  
M. J. Patel ◽  
H. L. Kacha
Keyword(s):  
Design Experiment ◽  
Farm Yard Manure ◽  
Blue Colour ◽  
College Of Agriculture ◽  
Titrable Acidity ◽  
Randomized Design ◽  
Covering Material ◽  
Completely Randomized Design ◽  
Horticultural Research

Aims: To study the post harvest shooting namely; control, humic acid 2%, 2, 4-D 30 mg/l, gibberellic acid (GA3) 100 mg/l, CPPU 4 mg/l and sulphate of potash (SOP) 2% and covering materials viz., non- woven material bag and blue colour polyethylene sleeve (6% perforated) bag on bunch of banana cv. Willium Study Design: Experiment was laid out in a Completely Randomized Design (Factorial) with three repetitions. Place and Duration of Study: Experiment was carried out at the Horticultural Research Farm,Department of Horticulture, B. A. College of Agriculture,  AAU, Anand during the year 2017-18 and 2018-19 Methodology: The experimental plot was prepared by deep ploughing, harrowing and levelling. The pits of 30 x 30 x 30 cm were dug out at a spacing of 1.8 x 1.8 m2 and well decomposed fine textured Farm Yard Manure (FYM) at the rate of 10 kg per pit was applied at planting. Well hardened, healthy, uniform tissue cultured tissue culture plants of willium banana having 5-6 leaves were used for planting. Post shooting sprays were given twice i.e. 1st spray after complete opening of inflorescence and 2nd spray after 30 days of first spray with covering the bunch immediately after second spray.The fruits which were used for recording the weight loss during ripening, were used to calculating pulp: peel ratio. Pulp to peel ratio was calculated by dividing respective pulp weight by respective peel weight. The TSS value of the fruit was recorded by using hand refractometer having range of 0-32 ⁰Brix. Acidity (%) was calculated by the method described by Rangana (1977) was adopted for estimation of titrable acidity. Results: The results revealed that the banana bunches sprayingof CPPU 4 mg/lwith bunch covering with non woven materials were recorded significantly lower physiological loss in weight (12.80, 13.60 and 13.20%) and (13.26, 14.14 and 13.70%), respectively. Whereas, post shooting spray of SOP 2% with non-woven material bag coveringwere recorded significantly maximum TSS (21.42, 21.05 and 21.17 ºBrix; 21.39, 20.93 and 21.16 ºBrix), reducing sugar (12.55, 12.34 and 12.45%; 12.21, 12.16 and 12.18%) and total sugar (21.42, 20.82 and 21.12%; 20.84, 20.50 and 20.67%) as compared to rest of the treatments of post shooting sprays and bunch covering materials on banana quality. Conclusion: From the two years of field study, it can be concluded that the post shooting spraying of CPPU 4 mg/l and SOP 2% with non woven bunch covering bags were improve fruit quality of banana.

scholarly journals Study of antioxidant activity and anti-cholesterol content on red yeast rice with substrates variation (rice, corn and dried cassava)

2011 ◽  
Vol 9 (2) ◽  
pp. 38-44
Author(s):  
HADI WIYOTO ◽  
M. A.M. ANDRIANI ◽  
NUR HER RIYADI PARNANTO
Keyword(s):  
Antioxidant Activity ◽  
Amylose Content ◽  
Cholesterol Content ◽  
The Other ◽  
Red Yeast Rice ◽  
Red Yeast ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Level Of Significance ◽  
High Amylose

Wiyoto H, Andriani MAM, Parnanto NHR. 2011. Study of antioxidant activity and anti-cholesterol content on red yeast rice with substrates variation (rice, corn and dried cassava). Biofarmasi 9: 38-44. Red yeast rice is one of fermented rice product by Monascus purpureus. Traditionally, the substrate used to produce red yeast rice is rice. Usually, the rice with high amylose content is proper to produce red yeast rice than low amylose. The other substrates that be used to produce red yeast rice are corn and dried cassava. The purposes of this research were to determine the effect of substrates variation (rice, corn and dried cassava) on antioxidant activity and anti-cholesterol content in red yeast rice, and to determine the substrate(s) that produce the highest antioxidant activity and anticholesterol content. The design of this research was a Completely Randomized Design with one factor, i.e. the kind of substrates: rice, corn and dried cassava, with three replications. Then, the data were analyzed with ANOVA at a level of significance α=0.05, and continued with DMRT at the same level. This results showed that the effect of substrates kind to antioxidant activity and anti-cholesterol content on red yeast rice. The rice substrate had higher antioxidant activity and anti-cholesterol content than corn and dried cassava substrates. The antioxidant activity and the anti-cholesterol content on red yeast rice from rice substrate were 45.6100% and 0.026600%, respectively. The antioxidant activity and the anti-cholesterol content on red yeast rice from corn substrate were 44.0500% and 0.022833%, respectively, while the antioxidant activity and the anti-cholesterol content on red yeast rice from dried cassava substrate were 42.8333% and 0.013200%, respectively.

scholarly journals Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. N. Mahadi ◽  
F. Zawawi ◽  
R. Nulit ◽  
M. H. Ibrahim ◽  
N. I. Ab. Ghani
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Germination Percentage ◽  
Leaf Length ◽  
Seed Vigor ◽  
Rice Seed ◽  
Combination Treatments ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

scholarly journals THE EFFECT OF CORONA INCANDESCENT PLASMA RADIATION AND STORAGE TEMPERATURE ON THE VIABILITY OF GARLIC SEEDS (ALLIUM SATIVUM L. LUMBU KUNING VARIETY)

2021 ◽  
Vol 5 (1) ◽  
pp. 11-17
Author(s):  
Febiasasti Trias Nugraheni ◽  
Erma Prihastanti ◽  
Endah Dwi Hastuti
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Storage Temperature ◽  
Germination Percentage ◽  
Plasma Radiation ◽  
Agricultural Commodity ◽  
Time Duration ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Storage

Garlic (Allium sativum L.) is an agricultural commodity which is widely consumed by the community. The obstacle experienced in cultivation is that a relatively long dormancy period of about 6-7 months. The dormancy of garlic seeds occurs because the embryo has not yet been fully formed, and the growth hormone and inhibitory hormone are not balanced. The methods used to break dormancy and accelerate germination are by using corona incandescent plasma radiation and storage temperature. The research utilized factorial Completely Randomized Design (CRD), consisting of six treatments with five replications. The first factor was the corona incandescent plasma radiation carried out on the garlic seeds ‘Lumbu Kuning’ variety with time duration of 0, 15, and 30 minutes. The second factor was storage temperature comprising of room temperature and cold temperature of 7oC stored for 30 days. Viability parameters observed are germination percentage dan the length of sprouts. The data were analyzed by using ANOVA followed by the DMRT test at the 95% confidence level. The results showed that corona incandescent plasma radiation influenced the germination percentage using plasma radiation for 15- 30 minutes.

scholarly journals APPLICATION OF BENZYLADENINE (BA) IN THE FORM OF LANOLIN PASTE ON FLOWER-STALK BUDS SUCCESSFULLY INDUCED RE-BLOOMING OF HYBRID Phalaenopsis

2020 ◽  
Vol 8 (2) ◽  
pp. 383
Author(s):  
Mukhaila Iryani ◽  
Yusnita Yusnita ◽  
Dwi Hapsoro ◽  
Kukuh Setiawan ◽  
Agus Karyanto
Keyword(s):  
Plant Growth ◽  
The Other ◽  
Control Treatment ◽  
Bud Break ◽  
Flower Stalk ◽  
Moth Orchid ◽  
Randomized Design ◽  
Time Period ◽  
Long Time ◽  
Completely Randomized Design

Hybrid moth orchid (genus Phalaenopsis) is one of the most popular ornamentals in Indonesia. It has beautiful and long-lasting flowers, but cultivating this orchid is still become a challenging issues due to the need of specific condition to grow and long time period to re-blooming. Plant growth regulators (PGR) (i.e. Benzyladenine (BA)) has been widely documented as a flower-inducing substance in several orchids. However, the optimal concentration and its mechanism in inducing flower-stalk bud and re-blooming is still unclear. This research aimed to study the effects of BA application in the form of lanolin paste on hybrid Phalaenopsis flower-stalk buds. We conducted this study using completely randomized design with four replications at the greenhouse laboratory Faculty of Agriculture University of Lampung on August to December 2018. We divided the orchid into 5 group of BA concentration (0, 1000, 1500, 3000, or 6000 ppm). The sheath of the fourth or fifth buds of the flowers were carefully opened, then it smeared with BA. The percentage of bud break into flower spike or keiki, length of shoots or spike and number of open flowers were recorded until 10 weeks of observation. The results showed that, neither of the buds under the control treatment (without BA), 1000 ppm nor 1500 ppm BA broke and grew into keiki or spike. On the other hand, application of BA at 3000 ppm or 6000 ppm successfully induced 100% flower spikes on the buds treated. No keiki was formed in all buds treated. In addition, treatment of the buds with 6000 ppm BA produced longer flower spikes as well as more open flowers. We conclude that the application of BA (minimal 3000 ppm) successfully induced flower spike of  hybrid Phalaenopsis.

Sign in / Sign up

Export Citation Format

Share Document