scholarly journals mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil

2022 ◽  
Vol 52 (3) ◽  
Author(s):  
José Givanildo da Silva ◽  
Anderson Carlos Camargo ◽  
Renata Pimentel Bandeira de Melo ◽  
Breno Bezerra Aragão ◽  
Junior Mário Baltazar de Oliveira ◽  
...  
Keyword(s):  
Restriction Analysis ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Pulsed Field Gel Electrophoresis ◽  
Cow Milk ◽  
Dairy Production ◽  
Microbiological Analysis ◽  
Production Environment ◽  
Genetic Profiles ◽  
Staphylococcus Spp

ABSTRACT: This study detected the presence and distribution of mecA in Staphylococcus spp. in the dairy production environment at farm level in Brazil. We analyzed 335 samples of mastitis cow milk, 15 samples of nostrils and hand swabs from milkers, 14 teat cup swabs, and 9 milking buckets swabs. Initially, the samples were subjected to microbiological analysis to detect Staphylococcus spp. and then S. aureus and mecA positive isolates were identified by PCR. All S. aureus isolates carrying the mecA genes were subjected to DNA macro-restriction analysis by Pulsed-Field Gel Electrophoresis (PFGE). The mecA gene was detected in 6/335 (1.78%) of mastitis cow milk, 5/15 (33.3%), and 5/15 (33.3%) of nostrils and hand swab, and 4/14 (28.5%) of the teat cup isolates. MRSA genotyping was performed by PFGE, a total of seven pulsotypes were grouped in two clusters. This study identified the occurrence and spread of MRSA at dairy environment of farms, and also the existence of distinct genetic profiles between isolates.

scholarly journals Phenotypic and Genotypic Characteristics and Resistance Profile of Staphylococcus spp. from Bovine Mastitis

2020 ◽  
Vol 48 ◽  
Author(s):  
Érica Chaves Lucio ◽  
Gisele Veneroni Gouveia ◽  
Mateus Matiuzzi Da Costa ◽  
Mário Baltazar De Oliveira ◽  
Rinaldo Aparecido Mota ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Milk Production ◽  
Biofilm Formation ◽  
Negative Impact ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Resistant Bacteria ◽  
Production Chain ◽  
Biofilm Production ◽  
Staphylococcus Spp

Background: Bovine mastitis remains one of the health problems that cause the most damage to milk producers. The negative impact of mastitis is due to reduced milk production, early slaughter of females, reduced commercial value of the affected animals, losses in the genetic potential of the herd, expenses with medicines and veterinary medical assistance. Staphylococcus spp. stands out as the cause of this disease and has been able to remain in the mammary gland, becoming resistant to several antimicrobials. The aims of the present study were to characterize the phenotypes, genotypes and resistance profiles of Staphylococcus spp. isolates from bovine mastitis cases in the state of Pernambuco, Brazil.Materials, Methods & Results: These isolates were classified according to biochemical tests and the presence of the nuc gene.  The polymerase chain reaction (PCR) for amplification of the mecA and blaZ genes was used to analyze the genetic potentials of antimicrobial resistance. Isolates were also phenotypically tested for resistance to nine antimicrobials (ampicillin, doxicillin, erythromycin, gentamicin, rifampicin, cephalothin, amoxicillin, nalidixic acid and oxacillin). The genetic potentials for biofilm production were evaluated by the amplifications of the icaD, icaA and bap genes. The phenotypic test of gentian violet was used for biofilm formation analyzes. Ninety-three (93.0%) of the isolates among the Staphylococcus spp. samples were classified as Staphylococcus aureus. The lowest percentage of sensitivity observed was for amoxicillin (28.0%). All of the isolates were sensitive to erythromycin and gentamicin, and 15 (15%) exhibited sensitivity to all of the drugs tested. All of the isolates were negative for the mecA gene, and 36 (36%) were positive for blaZ. In the adhesion microplate tests, 44 (44%) of the isolates were capable of biofilm formation. Of these, seven (15.9%) were strong formers, whereas 16 (36.3%) and 21 (47.8%) were moderate and weak formers, respectively. The icaD gene was confirmed in 89 (89%) of the isolates. The icaA gene was confirmed in 61 (61%) samples, and the bap gene in 52 (52%) samples. One of the samples did not possess icaA, icaD or bap and exhibited moderate biofilm formation according to the microplate adherence test. Sixteen isolates simultaneously exhibited the three genes tested for biofilm production (icaA, icaD and bap) and were negative according to the microplate adherence test.Discussion:  The indiscriminate use of antibiotics to treat mastitis is a common practice in the study area, which may have contributed to the high proportion of herds (88.23%; 15/17) with multi-resistant isolates, constituting a selection factor for the dissemination of resistant bacteria among herds.  The absence of the mecA gene in the present study may be associated with the development of resistant bacteria through another mechanism, such as the overproduction of beta-lactamases. The results demonstrate that antimicrobial resistance occurs in Staphylococcus spp. that cause bovine mastitis in herds of Pernambuco and that these isolates have the a great capacity for biofilm formation. It is necessary to sensitize the professionals involved in the milk production chain of Brazil regarding the importance of the adequate use of antimicrobials for the treatment and control of mastitis, since studies in the country indicate the dissemination of resistant bacterial strains.

scholarly journals Antimicrobial Susceptibility Profile of Bacteria in Mastitis Cow Milk Samples

2021 ◽  
Vol 78 (1) ◽  
pp. 96
Author(s):  
George Cosmin NADĂȘ ◽  
Cristiana Ștefania NOVAC ◽  
Cosmina Maria BOUARI ◽  
Ioana Adriana MATEI ◽  
Nicodim Iosif FIȚ
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bovine Mastitis ◽  
Cow Milk ◽  
Bacterial Strains ◽  
Disk Diffusion Test ◽  
Physical Trauma ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Bovine mastitis is a cause of major financial losses in the dairy industry worldwide. Bovine mastitis is defined as the inflammatory response of the udder tissue due to either physical trauma or infections caused by different microorganisms, especially bacteria. More than 135 pathogenic agents have been identified from bovine mastitis. The aim of the study was to identify the bacterial genera involved in mastitis etiology in cattle and to test the antimicrobial susceptibility to antibiotics. The samples were collected before morning milking and evaluated by microscopic and cultural examination, and further identified using biochemical methods. The evaluation of antimicrobial susceptibility was performed using Kirby-Bauer disk diffusion test. Overall, Staphylococcus spp., Bacillus spp., Streptococcus spp., Micrococcus spp., Escherichia spp. and Trueperella spp. were identified. The most frequent bacterial associations were observed for Staphylococcus-Bacillus, Bacillus-Streptococcus and Staphylococcus-Micrococcus. Regarding the antimicrobial sensitivity profile, the majority of the bacterial strains we have isolated were classified as sensitive, with the exception of Escherichia coli, with one strain being resistant to all tested antibiotics. Among the antimicrobials, the Amoxicillin and clavulanic acid was the most efficient one and Trimethoprim/Sulfamethoxazole did not exhibit an inhibitory activity in 19 milk samples.

scholarly journals Expression of genes encoding resistance in Staphylococcus spp. isolated from bovine subclinical mastitis in Brazil

2020 ◽  
Vol 14 (07) ◽  
pp. 772-780
Author(s):  
Eveline Zuniga ◽  
Nilson Roberti Benites ◽  
Aline Santana da Hora ◽  
Priscila Luiza Mello ◽  
Marco Antonio Laes ◽  
...  
Keyword(s):  
Methicillin Resistance ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Gene Encoding ◽  
Resistance Factors ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Staphylococcus Spp

Introduction: Staphylococci are the most important agents associated with bovine mastitis. This study aimed at characterizing resistance factors to antimicrobials in Staphylococcus spp. isolated from the milk of cows with subclinical mastitis. Methodology: In vitro resistance of 243 Staphylococcus spp. isolates to antimicrobials commonly used in clinical practice was evaluated. The detection and expression of genes encoding resistance mecA (gene encoding penicillin binding protein 2a) mecALGA251 (mecA homologue), blaZ (gene encoding penicillin resistance), femA and femB (genes encoding essential factors - A and B - for the expression of methicillin resistance) and aacA-aphD (gene encoding for a bifunctional enzyme that confers resistance to gentamicin) using PCR and RT-PCR was investigated. Results: One or more genes encoding resistance to different antimicrobials were detected in 184 Staphylococcus spp. samples. The femA and femB genes were the most frequent. Regarding the variables’ detection (N = number of strains) and expression (% of strains), the following results were obtained: blaZ (N = 40 – 82.5%), femA (N = 147 – 47.6%), aacAaphD (N = 30 – 43.3%), femB (N = 138 – 29.7%), mecA (N = 33 – 27.3%), mecALGA251 (N = 01 – 0.0%). There was a higher occurrence of phenotypic resistant strains for amoxicillin, ampicillin and penicillin in isolates positive for detection and/or expression of blaZ gene when compared with the other genes. Conclusions: The present study provides new information on genotypic traits of Staphylococcus isolates from bovine subclinical mastitis especially regarding the evaluation of expression of genes associated with antimicrobial resistance in Staphylococcus spp. using molecular tools.

The Effectiveness of Amoxicillin and Cefalexin in Two Species from Staphylococcus and Bacillus Isolated from Bovine Mastitis in Sudan

2019 ◽  
Author(s):  
Reem Rabie Mohammed Salih
Keyword(s):  
Bacillus Cereus ◽  
Bovine Mastitis ◽  
Bacillus Coagulans ◽  
Staph Aureus ◽  
Milk Samples ◽  
Sensitivity Tests ◽  
Bacillus Spp ◽  
Staphylococcus Spp

In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial agents. The percentage of isolates was as follows: Staphylococcus spp 52%, Bacillus spp 26%, Enteroccocispp 3%; microccus 3%, Nesseria 2%, Branhamella 3%, Clostridium 1%, Bordetella 2%, Enterobacteria 3%, Aeromonas 4%, Fusobacterium 1%. In sensitivity tests used two antibiotics Amoxicillin and Cephalexin against two highest isolates in this study (Staph spp and Bacillus spp and found: Staph. aureus and Staph. hyicus were resistant to Amoxicllin and cephlaxin relatively, Bacillus coagulans and Bacillus cereus were susceptible to Amoxicllin and cephlaxin.

scholarly journals In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

2017 ◽  
Vol 37 (4) ◽  
pp. 368-378 ◽  
Author(s):  
Jusciêne B. Moura ◽  
Agueda C. de Vargas ◽  
Gisele V. Gouveia ◽  
João J. de S. Gouveia ◽  
Juracy C. Ramos-Júnior ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activities ◽  
Meca Gene ◽  
Usnic Acid ◽  
Organic Extract ◽  
In Vitro Antimicrobial Activity ◽  
Low Concentrations ◽  
Wide Range ◽  
Staphylococcus Spp

ABSTRACT: Cladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.

scholarly journals Impairments of mecA gene detection in bovine Staphylococcus spp.

2014 ◽  
Vol 45 (3) ◽  
pp. 1075-1082 ◽  
Author(s):  
Dayanne Araújo de Melo ◽  
Irene da Silva Coelho ◽  
Cássia Couto da Motta ◽  
Anna Carolina Coelho Marín Rojas ◽  
Felipe Carlos Dubenczuk ◽  
...  
Keyword(s):  
Meca Gene ◽  
Gene Detection ◽  
Staphylococcus Spp

scholarly journals Microbiological analysis and sensitivity profile of Staphylococcus spp. in subclinical mastitis of dairy goats

2016 ◽  
Vol 68 (2) ◽  
pp. 336-344
Author(s):  
S.R.S. Salaberry ◽  
A.B.S. Saidenberg ◽  
E. Zuniga ◽  
F.F. Gonsales ◽  
P.A. Melville ◽  
...  
Keyword(s):  
Subclinical Mastitis ◽  
Economic Losses ◽  
Microbiological Analysis ◽  
Dairy Goats ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Resistant Strains ◽  
Staphylococcus Spp ◽  
Sensitivity Profile

Subclinical mastitis in goats causes economic losses and risks to public health. Given the need for research that shows the most isolated staphylococci species and sensibility tests comparing the resistance between coagulase-negative (CNS) and positive Staphylococcus (CPS) goats with subclinical mastitis, the aim of this study was to identify the microorganisms isolated from milk samples of goats with subclinical mastitis, as well as define the staphylococci species and determine the sensitivity profile of Staphylococcus spp. to antimicrobials. To collect samples, tests were performed for mug of black background and California mastitis test (CMT), collecting milk from CMT positive animals. A total of 226 samples from seven herds of dairy goats was collected and forwarded to the laboratory, where they were seeded for the isolation of the microorganism and implementing the antibiotic sensibility test. Of these, 122 samples had bacterial growth and the most isolated staphylococci species were: S. epidermidis (24.55%), S. lugdunensis (15.40%) and S. intermedius (13.64%). Samples showed increased resistance to antimicrobials: penicillin (81.8%), oxacillin (60.0%) and ampicillin (55.5%). Greater sensitivity to: enrofloxacin (99.1%), erythromycin (98.2%), gentamicin (98.2%) and vancomycin (98.2%) were observed. The S. epidermidis showed higher antimicrobial resistance to amoxicillin and penicillin than S. lugdunensis and S. intermedius. Similar resistance in vitro between CNS and CPS was observed to most antimicrobials. It is important to control the overuse of antibiotics to prevent the emergence of resistant strains.

scholarly journals Antimicrobial profile of multidrug-resistant Staphylococcus spp. isolated from bovine mastitis cases in the northwest region of Paran State, Brazil

2014 ◽  
Vol 8 (37) ◽  
pp. 3392-3397
Author(s):  
Luiz Pereira De Oliveira Jorge ◽  
Ratti da Silva Danilo ◽  
Fernanda De Oliveira Aliny ◽  
Luiza Mello Priscila ◽  
Olivotto Agostinis Renata ◽  
...  
Keyword(s):  
Bovine Mastitis ◽  
Multidrug Resistant ◽  
Northwest Region ◽  
Staphylococcus Spp

scholarly journals Microbiological analysis of commonly used toothpaste samples in Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 38-40
Author(s):  
Mousumi Talukder ◽  
Ifra Tun Nur
Keyword(s):  
Microbiological Quality ◽  
Enteric Bacteria ◽  
Fungal Species ◽  
Microbiological Analysis ◽  
Bacillus Spp ◽  
Viable Bacteria ◽  
Dental Diseases ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Great Public Health

Present study attempted to identify and enumerate microorganisms spoiling commonly used toothpaste samples. Among 7 brand of toothpaste, almost all were found to be rigorously contaminated with total viable bacteria within a range of 103 – 105 cfu/g. Proliferation of fungal species was observed up to 105 cfu/g. Prevalence of Staphylococcus spp., Pseudomonas spp. and Bacillus spp. was observed within a range of 103-105 cfu/g while Vibrio spp. were completely absent. Among the enteric bacteria, Escherichia coli and Klebsiella spp. was found in all the samples tested. Such findings highlighted a great public health risk associated with dental diseases among the users and thereby specified the importance to introduce a proper guideline in maintaining good microbiological quality for such daily usage healthcare products. Stamford Journal of Microbiology, Vol.8(1) 2018: 38-40

scholarly journals Detection of β-lactamase, blaZ and mecA in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Garanhuns, Brazil

2021 ◽  
Vol 15 (2) ◽  
pp. 140-145
Author(s):  
Amanda Pereira Lucas ◽  
Andriele Renata Barbosa de Farias ◽  
Elizabete Cristina da Silva ◽  
Kleber Régis Santoro ◽  
Marcelo Mendonça ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Meca Gene ◽  
Resistance Mechanism ◽  
Bovine Mastitis ◽  
Duplex Pcr ◽  
Genetic Determinants ◽  
The City

There are few reports in the literature about genetic determinants of resistance to β-lactams in Staphylococcus aureusisolated from dairy cattle located in the municipality of Garanhuns, state of Pernambuco, Brazil. Thus, this study aimed to investigate the production of β-lactamase and the presence of the blaZ and mecA genes in penicillin-resistant S. aureus isolated from cases of subclinical bovine mastitis in the city of Garanhuns. Forty-six strains of penicillin-resistant S. aureus were evalu-ated using the nitrocefin disc test and duplex PCR. The results revealed that 45 strains (97.8%) were positive for β-lactamase production and 44 (95.7%) carried the blaZ gene. Among the latter, 43 (97.7%) were β-lactamase producers and only one (2.3%) was not. The mecA gene was not detected in any of the isolates investigated. The results suggest that enzymatic inacti-vation is the main β-lactam resistance mechanism expressed by S. aureus in the herds analyzed.

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