scholarly journals Substrate disinfection methods on the production and nutritional composition of a wild oyster mushroom from the Amazon

2021 ◽  
Vol 45 ◽  
Author(s):  
Lorena Vieira Bentolila de Aguiar ◽  
Ceci Sales-Campos ◽  
Paula Romenya dos Santos Gouvêa ◽  
Bazilio Frasco Vianez ◽  
Eustáquio Souza Dias ◽  
...  

ABSTRACT One of the most expensive steps in mushroom production is the disinfection of the substrate. This study aimed to evaluate different methods of disinfecting various substrates used in the cultivation of Pleurotus ostreatus from the Amazon. P. ostreatus was grown under uncontrolled temperature conditions in a greenhouse (at approximately 30 °C) on substrates formulated with residues (seeds) of açaí and tucumã palm trees, Brazil nuts shell and pine sawdust. The different substrate disinfection treatments comprised autoclaving, immersion in hot water or immersion in calcium hydroxide (2%). The chemical composition of residues, substrates, and mushrooms was analyzed. During cultivation, it was found that in the treatment with hot water, all the bags were contaminated, and treatment with calcium hydroxide inhibited the colonization and growth of the mushrooms. P. ostreatus grown on the autoclaved açaí substrate supplemented with tucumã had higher production. The highest percentage of fiber content was obtained when the mushrooms were cultivated in the pine substrate supplemented with Brazil nuts (32.68 to 33.65%), and the highest protein content (20.03 to 20.88%) was found in the mushrooms grown on açaí-based substrates. Considering that P. ostreatus showed higher productivity in autoclaved substrates, further studies should be performed to develop alternative methods of disinfecting substrates for the cultivation of mushrooms in the Amazon region, as well as, in other regions with a tropical climate. However, the cultivation of this mushroom is promising using some regional substrates, especially with açaí, which is one of the important natural products of the Amazon.

2016 ◽  
Vol 4 (4) ◽  
pp. 52 ◽  
Author(s):  
Funda Atila

AbstractThe aim of this paper is to estimate which of disinfection method is more effective in case of higher yield and fewer cost in cultivation of Pleurotus ostreatus in rural areas.  For this purpose, four different treatments; scalping in hot water (60, 80 and 100°C), and chemical disinfection (1% formaldehyde) were compared with sterilization by autoclave. It has been noticed that method of disinfection affects mycelium growing duration(day), yield(g/kg), biological efficiency (BE%), avarage weight of fruitbody(g), diameter of pileus(cm), stipe lenght(cm) and width(cm).  P. ostreatus took significantly much time to colonise the substrate (19.9 days) disinfected by chemical disinfection compared with others. Chemical disinfection (271.28 g/kg and 91.96%) and scalding hot water at 80oC (263.42 g/kg and 90.83%) had highest productivity in terms of yield and BE, respectively, while scalding hot water at 60oC (136.08 g/kg and 46.92%) had least productivity. The largest diameter of fruitbody was obtained by scalding in hot water at 80oC, while the longest stipes were harvested in disinfected substrate by chemicals.  In conclusion, cheaper and more easily applecable scalping in hot water 80°C and chemical disinfection may be use for the disinfection of growing substrates instead of sterilization with autoclave in rural areas.Keywords: mushroom growing substrate, disinfection methods, autoclaved, scalding, chemical disinfection, Pleurotus ostreatus


2011 ◽  
Vol 415-417 ◽  
pp. 1265-1272
Author(s):  
Wen Biao Zhang ◽  
Wen Zhu Li ◽  
Bing Song Zheng

Miscanthus is a highly productive, rhizomatous, C4 perennial grass that should be considered as an excellent active carbon precursor. This paper compares the charcoal characterization and chemical composition between M. sinensis and M. floridulus. Species differed in water content, hot water extract, 1% NaOH extract, organic solvent extract, cellulose, lignin and ash. Carbonization temperatures have effects on charcoal yields of Miscanthus, which ranged from 23.5% to 48.0% for M. sinensis and 11.3% to 37.2% for M. floridulus. Water content, charcoal density, pH value, and specific surface area of charcoal characterization varied between two species of Miscanthus. The specific surface area increased with the increase of carbonization temperature. The highest specific surface area of M. sinensis and M. floridulus was 351.74 m2g−1and 352.74 m2g−1, respectively, when the carbonization temperature was 800°C.


2017 ◽  
Vol 100 ◽  
pp. 9-18 ◽  
Author(s):  
Bárbara R. Cardoso ◽  
Graziela B. Silva Duarte ◽  
Bruna Z. Reis ◽  
Silvia M.F. Cozzolino

Author(s):  
Ade Chandra Iwansyah ◽  
Rizal M. Damanik ◽  
Lilik Kustiyah ◽  
Muhammad Hanafi

<p><strong>Objective: </strong>The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.</p><p><strong>Methods: </strong>A wide range of analytical parameters was studied, including yield; pH; water activity (a<sub>w</sub>); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.</p><p><strong>Results: </strong>All of the galactagogue herbs, <em>viz</em>. <em>Plectranthus amboinicus </em>(Lour.) (PA), <em>Sauropus androgynus</em> (SA), <em>Moringa oleifera</em> (MO), and <em>Carica papaya</em> leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC<sub>50</sub>), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO&gt;CA&gt;PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.<strong></strong></p><p><strong>Conclusion: </strong>This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.</p>


2020 ◽  
Author(s):  
Om Kolhe ◽  
Aidan Moy

Freshwater acidification is the result of acid rain precipitating over a freshwater body. There are significant ecological consequences that result from such precipitation, as ecosystems surrounding freshwater bodies are destroyed because many of the organisms in these ecosystems cannot tolerate the pH change caused by acid rain. Current solutions involve a technique known as liming, where powdered calcium carbonate is added to freshwater bodies creating a buffer that helps neutralize the acidity caused by acid rain. However, scientists have been searching for alternative methods to combat the decrease in pH caused by freshwater acidification, by using substitute compounds. In this experiment, we test the efficacy of alternate solutions involving montmorillonite and calcium hydroxide when compared to the currently employed method of using calcium carbonate to combat acidification.


2008 ◽  
Vol 18 (12) ◽  
pp. 1723-1727 ◽  
Author(s):  
Jae-Young Cha ◽  
Jung-Wook Kim ◽  
Bo-Kyung Park ◽  
Hyun-Jin Jin ◽  
Sung-Young Kim ◽  
...  

BioResources ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 816-824
Author(s):  
Jonathan Sergio Chávez-Rosales ◽  
Luis Fernando Pintor-Ibarra ◽  
Nicolás González-Ortega ◽  
Rocio Orihuela-Equihua ◽  
Faustino Ruiz-Aquino ◽  
...  

The basic chemical composition and calorific value of 19 samples of pine sawdust from different forest industries located in five states of the Mexican Republic (Chihuahua, Michoacán, Durango, Oaxaca, and Nuevo León) were determined. The results obtained ranged as follows: total extractives (6.1% to 23.4%), holocellulose (60.1% to 70.4%), lignin (20.5% to 25.8%), ash (0.27% to 0.95%), pH (4.1 to 5.3), and calorific value (20.1 MJ/kg to 21.0 MJ/kg). Except for the ash content, significant statistical differences were found according to the origin of the pine sawdust samples. Based on the results obtained, the sawdust biomass has the potential to obtain densified solid biofuels.


1970 ◽  
Vol 20 (2) ◽  
pp. 483-487
Author(s):  
LF Zitte ◽  
RS Konya

Antinociceptive is reducing sensitivity to painful stimuli for the individual. The objective of this study was to evaluate the antinociceptive potentials of graded doses of Pleurotus ostreatus (Oyster Mushroom) aqueous extract in Rattus norvegicus (Albino Rats) and its chemical pattern by comparing it with a standard drug and a control using the hot water based flick tail test. Thirty five adult rats of both sexes were used for the experiment which, were divided into five groups of seven rats per group. Group one was used as the control (with 1ml normal saline), while groups two, three and four were treated with 100, 200 and 400 mg/kg of Pleurotus ostreatus aqueous extracts and group five treated with 15 mg/kg Aspirin (a standard drug) as positive control. Hot water at 550C was used to determine the nociceptive responses of the animals to detect anti-nociceptive effects of Pleurotus ostreatus extracts as compared to the control in hot water inflicted pain. The results suggested that Pleurotus ostreatus aqueous extract exhibits antinociceptive properties against thermal stimulus at 550C. The diversity of individual animals’ pain tolerance threshold when immersed in hot water was also observed during the experiment. However, the extract indicated a high degree of anti-nociceptive effect at 30, 60, 90 and 120 minutes post treatment period, with a progressively longer threshold time for pain sensitivity. It was also found that at 90 minute period, the control portrayed a relatively short response time.KEY WORDS: Anti-nociceptive, threshold pain, Pleurotus ostreatus, hot water test flick tail.


Sign in / Sign up

Export Citation Format

Share Document