scholarly journals Effect of Different substrate Disinfection Methods on the Production of Pleurotus ostreatus

2016 ◽  
Vol 4 (4) ◽  
pp. 52 ◽  
Author(s):  
Funda Atila
Keyword(s):  
Rural Areas ◽  
Pleurotus Ostreatus ◽  
Hot Water ◽  
Biological Efficiency ◽  
Disinfection Methods ◽  
Chemical Disinfection

AbstractThe aim of this paper is to estimate which of disinfection method is more effective in case of higher yield and fewer cost in cultivation of Pleurotus ostreatus in rural areas.  For this purpose, four different treatments; scalping in hot water (60, 80 and 100°C), and chemical disinfection (1% formaldehyde) were compared with sterilization by autoclave. It has been noticed that method of disinfection affects mycelium growing duration(day), yield(g/kg), biological efficiency (BE%), avarage weight of fruitbody(g), diameter of pileus(cm), stipe lenght(cm) and width(cm).  P. ostreatus took significantly much time to colonise the substrate (19.9 days) disinfected by chemical disinfection compared with others. Chemical disinfection (271.28 g/kg and 91.96%) and scalding hot water at 80oC (263.42 g/kg and 90.83%) had highest productivity in terms of yield and BE, respectively, while scalding hot water at 60oC (136.08 g/kg and 46.92%) had least productivity. The largest diameter of fruitbody was obtained by scalding in hot water at 80oC, while the longest stipes were harvested in disinfected substrate by chemicals.  In conclusion, cheaper and more easily applecable scalping in hot water 80°C and chemical disinfection may be use for the disinfection of growing substrates instead of sterilization with autoclave in rural areas.Keywords: mushroom growing substrate, disinfection methods, autoclaved, scalding, chemical disinfection, Pleurotus ostreatus

scholarly journals Substrate disinfection methods on the production and nutritional composition of a wild oyster mushroom from the Amazon

2021 ◽  
Vol 45 ◽  
Author(s):  
Lorena Vieira Bentolila de Aguiar ◽  
Ceci Sales-Campos ◽  
Paula Romenya dos Santos Gouvêa ◽  
Bazilio Frasco Vianez ◽  
Eustáquio Souza Dias ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Calcium Hydroxide ◽  
Pleurotus Ostreatus ◽  
Nutritional Composition ◽  
Hot Water ◽  
Alternative Methods ◽  
Pine Sawdust ◽  
Brazil Nuts ◽  
Disinfection Methods ◽  
Palm Trees

ABSTRACT One of the most expensive steps in mushroom production is the disinfection of the substrate. This study aimed to evaluate different methods of disinfecting various substrates used in the cultivation of Pleurotus ostreatus from the Amazon. P. ostreatus was grown under uncontrolled temperature conditions in a greenhouse (at approximately 30 °C) on substrates formulated with residues (seeds) of açaí and tucumã palm trees, Brazil nuts shell and pine sawdust. The different substrate disinfection treatments comprised autoclaving, immersion in hot water or immersion in calcium hydroxide (2%). The chemical composition of residues, substrates, and mushrooms was analyzed. During cultivation, it was found that in the treatment with hot water, all the bags were contaminated, and treatment with calcium hydroxide inhibited the colonization and growth of the mushrooms. P. ostreatus grown on the autoclaved açaí substrate supplemented with tucumã had higher production. The highest percentage of fiber content was obtained when the mushrooms were cultivated in the pine substrate supplemented with Brazil nuts (32.68 to 33.65%), and the highest protein content (20.03 to 20.88%) was found in the mushrooms grown on açaí-based substrates. Considering that P. ostreatus showed higher productivity in autoclaved substrates, further studies should be performed to develop alternative methods of disinfecting substrates for the cultivation of mushrooms in the Amazon region, as well as, in other regions with a tropical climate. However, the cultivation of this mushroom is promising using some regional substrates, especially with açaí, which is one of the important natural products of the Amazon.

Growth and yield impact of oyster mushroom Pleurotus ostreatus (Jacq P. Kumm) cultivated on different agricultural wastes

2021 ◽  
Vol 27 (01) ◽  
pp. 2225-2233
Author(s):  
M. O. Agba ◽  
◽  
A. A. Markson ◽  
J. O. Oni ◽  
G. A. Bassey ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Growth Parameters ◽  
Total Yield ◽  
Fruit Body ◽  
Food Product ◽  
Dry Weight ◽  
Edible Mushroom ◽  
Growth And Yield ◽  
Biological Efficiency ◽  
Essential Food

Pleurotus ostreatus, an edible mushroom is an essential food product. Recognised as one of the cheapest sources of proteins, their high nutritional and anti-nutritional properties are of immense importance to humans. The present study evaluated the effect of sawdust and dried plantain leaves on the growth and yield of Pleurotus ostreatus. Sawdust and dried plantain leaves were composted into seven substrates; as single substrates and at different combinations. The composted substrates include sawdust 100 % (M0), Dry plantain leaves 100% (M1), Dry plantain leaves + sawdust at the ratio of 1:4 (M2), 2:3 (M3), 3:2 (M4), 1:1 (M5) and 4:1(M6). Growth parameters of interest assessed flush include mycelia running rate (MRR), area of pileus, length of the stipe, the girth of the stipe, fresh weight and dry weight, number of fruit body, total yield and biological efficiency. At the end of the three weeks spawn running period, M0 was overall best in supporting mycelia running rate with a mean MRR of 16.00 cm. M1 substrates produced mushroom with longer and bigger stipes (7.17 cm) at the first flush. A higher number of fruit bodies (82.66), total yield (130.35 g), and biological efficiency (43.45 g) were all seen in M0 substrates. Sawdust at 100 % (M0) proved to be the best substrate for the cultivation of Pleurotus ostreatus.

scholarly journals Antinociceptive effect of Pleurotus ostreatus (Oyster Mushroom) Aqueous Extracts in Rattus norvegicus (Albino Rats)

1970 ◽  
Vol 20 (2) ◽  
pp. 483-487
Author(s):  
LF Zitte ◽  
RS Konya
Keyword(s):  
Aqueous Extract ◽  
Rattus Norvegicus ◽  
Pleurotus Ostreatus ◽  
Hot Water ◽  
Oyster Mushroom ◽  
Albino Rats ◽  
Thermal Stimulus ◽  
Aqueous Extracts ◽  
Standard Drug ◽  
Nociceptive Responses

Antinociceptive is reducing sensitivity to painful stimuli for the individual. The objective of this study was to evaluate the antinociceptive potentials of graded doses of Pleurotus ostreatus (Oyster Mushroom) aqueous extract in Rattus norvegicus (Albino Rats) and its chemical pattern by comparing it with a standard drug and a control using the hot water based flick tail test. Thirty five adult rats of both sexes were used for the experiment which, were divided into five groups of seven rats per group. Group one was used as the control (with 1ml normal saline), while groups two, three and four were treated with 100, 200 and 400 mg/kg of Pleurotus ostreatus aqueous extracts and group five treated with 15 mg/kg Aspirin (a standard drug) as positive control. Hot water at 550C was used to determine the nociceptive responses of the animals to detect anti-nociceptive effects of Pleurotus ostreatus extracts as compared to the control in hot water inflicted pain. The results suggested that Pleurotus ostreatus aqueous extract exhibits antinociceptive properties against thermal stimulus at 550C. The diversity of individual animals’ pain tolerance threshold when immersed in hot water was also observed during the experiment. However, the extract indicated a high degree of anti-nociceptive effect at 30, 60, 90 and 120 minutes post treatment period, with a progressively longer threshold time for pain sensitivity. It was also found that at 90 minute period, the control portrayed a relatively short response time.KEY WORDS: Anti-nociceptive, threshold pain, Pleurotus ostreatus, hot water test flick tail.

scholarly journals Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2740 ◽  
Author(s):  
Ekaterina Baeva ◽  
Roman Bleha ◽  
Ekaterina Lavrova ◽  
Leonid Sushytskyi ◽  
Jana Čopíková ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Pleurotus Ostreatus ◽  
Cold Water ◽  
Water Soluble ◽  
Hot Water ◽  
Biologically Active ◽  
Alkaline Extract ◽  
A Cell ◽  
Oyster Mushrooms

Oyster mushrooms are an interesting source of biologically active glucans and other polysaccharides. This work is devoted to the isolation and structural characterization of polysaccharides from basidiocarps of the cultivated oyster mushroom, Pleurotus ostreatus. Five polysaccharidic fractions were obtained by subsequent extraction with cold water, hot water and two subsequent extractions with 1 m sodium hydroxide. Branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan predominated in cold- and hot-water-soluble fractions, respectively. Alternatively, these polysaccharides were obtained by only hot water extraction and subsequent two-stage chromatographic separation. The alkali-soluble parts originating from the first alkali extraction were then fractionated by dissolution in dimethyl sulfoxide (DMSO). The polysaccharide insoluble in DMSO was identified as linear (1→3)-α-d-glucan, while branched (1→3)(1→6)-β-d-glucans were found to be soluble in DMSO. The second alkaline extract contained the mentioned branched β-d-glucan together with some proteins. Finally, the alkali insoluble part was a cell wall complex of chitin and β-d-glucans.

Obtaining extracts rich in antioxidant polysaccharides from the edible mushroom Pleurotus ostreatus using binary system with hot water and supercritical CO2

2020 ◽  
Vol 330 ◽  
pp. 127173 ◽  
Author(s):  
Jhonatas Rodrigues Barbosa ◽  
Maurício Madson S. Freitas ◽  
Luã Caldas Oliveira ◽  
Luiza Helena S. Martins ◽  
Andryo Orfi Almada-Vilhena ◽  
...  
Keyword(s):  
Binary System ◽  
Pleurotus Ostreatus ◽  
Supercritical Co2 ◽  
Edible Mushroom ◽  
Hot Water

scholarly journals Influence of Calcium Silicate on the Chemical Properties of Pleurotus ostreatus var. florida (Jacq.) P. Kumm

2020 ◽  
Vol 6 (4) ◽  
pp. 299
Author(s):  
Rossana V. C. Cardoso ◽  
Márcio Carocho ◽  
Ângela Fernandes ◽  
Diego Cunha Zied ◽  
Juan Diego Valenzuela Cobos ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Pleurotus Ostreatus ◽  
Calcium Silicate ◽  
Insect Pests ◽  
Chemical Properties ◽  
Biological Efficiency ◽  
Vitamin D2 ◽  
Fruiting Bodies ◽  
Productivity Rate ◽  
The Impact

Supplementation of mushroom substrates has been linked to a higher resistance against insect pests, although few studies show the impact of this supplementation on the different agronomical parameters of mushrooms or even their chemical composition. In this work, the variation in the biological and chemical composition of oyster mushroom (Pleurotus ostreatus var. florida (Jacq.) P. Kumm) was analysed after varying the substrate supplementation of calcium silicate (0, 0.5, 1, 2, and 4% (w/w%)) during two harvest flushes. Overall, supplementation did not change the weight, the number of fruiting bodies, biological efficiency, yield ratio, and productivity rate of the mushrooms, although the harvest flushes did show significant differences. Furthermore, slight changes were found in the chemical composition with an increase in vitamin D2 and tocopherols for the mushrooms with higher amounts of calcium silicate. Overall, the substrate supplementation did not seem to induce expressive changes or decrease production yields, and can, therefore, continue to be researched as a potential application to fight agronomical pests.

scholarly journals Analysis of heat source selection for residential buildings in rural areas

2018 ◽  
Vol 10 ◽  
pp. 02034 ◽  
Author(s):  
Tomasz Szul
Keyword(s):  
Energy Efficiency ◽  
Rural Areas ◽  
Residential Buildings ◽  
Hot Water ◽  
Single Family ◽  
Output Capacity ◽  
Source Selection ◽  
Energy Needs ◽  
Actual Use ◽  
The Mean

The research aiming to check whether the output of currently installed boilers matches the use requirements together with estimation of their energy efficiency was carried out on a group of 84 single-family residential buildings located in rural areas. Heating and hot water energy needs were calculated for each building in order to determine the use requirements. This enabled verification whether the currently installed boilers match the actual use requirements in the buildings. Based on the calculations it was determined that the designed average boiler output in the group of buildings subject to analysis is 15.7 kW, whereas the mean rated output capacity of boilers installed therein is 25.4 kW. On average, the output capacity of the installed boilers exceeds the use requirements for the buildings by 60%. To calculate the energy efficiency of boilers, the mean annual boiler output capacity use coefficient was determined. For boilers selected on the basis of standard calculations, the mean coefficient is 0.47. For boilers currently in use it is 0.31, less than the above figure. The above calculations show that if boilers were correctly selected in compliance to the building needs, then the average estimated seasonal efficiency of 65% would be feasible. However, in the current state the achievable efficiency is approx. 55%.

scholarly journals Evaluation of Using Spent Mushroom Sawdust Wastes for Cultivation of Auricularia polytricha

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1892
Author(s):  
Chiu-Yeh Wu ◽  
Chih-Hung Liang ◽  
Zeng-Chin Liang
Keyword(s):  
Fruiting Body ◽  
Pleurotus Ostreatus ◽  
Pleurotus Eryngii ◽  
Biological Efficiency ◽  
Main Ingredient ◽  
Auricularia Polytricha ◽  
Pleurotus Cystidiosus

The purpose of this study was to investigate the suitability of different spent mushroom sawdust wastes (SMSWs) and different proportions of SMSWs as potential substrates for the cultivation of Auricularia polytricha by evaluating yield and biological efficiency of the fruiting body. Nine SMSWs were respectively utilized as the main ingredient in the cultivation of A. polytricha. Then, spent Pleurotus eryngii, Pleurotus cystidiosus, and Pleurotus ostreatus sawdust wastes were screened among these nine SMSWs to be utilized as substrate and to determine the suitable proportion of SMSW in the cultivation of A. polytricha based on their yields and biological efficiencies. The highest yield and biological efficiency (total of two flushes) of A. polytricha cultivation on a single SMSW substrate was obtained with spent P. eryngii sawdust waste, followed by spent P. cystidiosus and P. ostreatus sawdust wastes. These three SMSWs were then applied in nine combination substrates, which were screened based on yield and biological efficiency for cultivation of A. polytricha. The combination substrate with the highest yield and biological efficiency of A. polytricha cultivation was P. eryngii (PES) + P. cystidiosus spent sawdust (PCYS) (235.4 g/bag yield and 58.85% biological efficiency); its yield was 1.62 folds higher than that of the control. From the results, we found that it was feasible to use spent sawdust wastes of P. eryngii and P. cystidiosus to replace sawdust for cultivation of A. polytricha.

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