scholarly journals Prior intake of Brazil nuts attenuates renal injury induced by ischemia and reperfusion

2018 ◽  
Vol 40 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Natassia Alberici Anselmo ◽  
Leticia Colombo Paskakulis ◽  
Renata Correia Garcias ◽  
Fernanda Fortuci Resende Botelho ◽  
Giovana Queda Toledo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Creatinine Clearance ◽  
Renal Injury ◽  
Ischemia Reperfusion ◽  
Antioxidant Properties ◽  
Kidney Injury ◽  
Urinary Output ◽  
Left Renal Artery ◽  
And Oxidative Stress

ABSTRACT Introduction: Ischemia-reperfusion (IR) injury results from inflammation and oxidative stress, among other factors. Because of its anti-inflammatory and antioxidant properties, the Brazil nut (BN) might attenuate IR renal injury. Objective: The aim of the present study was to investigate whether the intake of BN prevents or reduces IR kidney injury and inflammation, improving renal function and decreasing oxidative stress. Methods: Male Wistar rats were distributed into six groups (N=6/group): SHAM (control), SHAM treated with 75 or 150 mg of BN, IR, and IR treated with 75 or 150 mg of BN. The IR procedure consisted of right nephrectomy and occlusion of the left renal artery with a non-traumatic vascular clamp for 30 min. BN was given daily and individually for 7 days before surgery (SHAM or IR) and maintained until animal sacrifice (48h after surgery). We evaluated the following parameters: plasma creatinine, urea, and phosphorus; proteinuria, urinary output, and creatinine clearance; plasmatic TBARS and TEAC; kidney expression of iNOS and nitrotyrosine, and macrophage influx. Results: Pre-treatment with 75 mg of BN attenuated IR-induced renal changes, with elevation of creatinine clearance and urinary output, reducing proteinuria, urea, and plasmatic phosphorus as well as reducing kidney expression of iNOS, nitrotyrosine, and macrophage influx. Conclusion: Low intake of BN prior to IR-induced kidney injury improves renal function by inhibition of macrophage infiltration and oxidative stress.

Trehalose attenuates renal ischemia-reperfusion injury by enhancing autophagy and inhibiting oxidative stress and inflammation

2020 ◽  
Vol 318 (4) ◽  
pp. F994-F1005
Author(s):  
Suwen Liu ◽  
Yunwen Yang ◽  
Huiping Gao ◽  
Ning Zhou ◽  
Peipei Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Renal Injury ◽  
Ischemia Reperfusion ◽  
Kidney Injury ◽  
Renal Ischemia ◽  
Renal Histology ◽  
Hypoxia Reoxygenation

Renal ischemia-reperfusion (IR) injury is one of the most common acute kidney injuries, but there is still a lack of effective treatment in the clinical setting. Trehalose (Tre), a natural disaccharide, has been demonstrated to protect against oxidative stress, inflammation, and apoptosis. However, whether it could protect against IR-induced renal injury needs to be investigated. In an in vivo experiment, C57BL/6J mice were pretreated with or without Tre (2 g/kg) through a daily single intraperitoneal injection from 3 days before renal IR surgery. Renal function, apoptosis, oxidative stress, and inflammation were analyzed to evaluate kidney injury. In an in vitro experiment, mouse proximal tubular cells were treated with or without Tre under a hypoxia/reoxygenation condition. Western blot analysis, autophagy flux detection, and apoptosis assay were performed to evaluate the level of autophagy and antiapoptotic effect of Tre. The in vivo results showed that the renal damage induced by IR was ameliorated by Tre treatment, as renal histology and renal function were improved and the enhanced protein levels of kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin were blocked. Moreover, autophagy was activated by Tre pretreatment along with inhibition of the IR injury-induced apoptosis, oxidative stress, and inflammation. The in vitro results showed that Tre treatment activated autophagy and protected against hypoxia/reoxygenation-induced tubular cell apoptosis and oxidative stress. Our results demonstrated that Tre protects against IR-induced renal injury, possibly by enhancing autophagy and blocking oxidative stress, inflammation, and apoptosis, suggesting its potential use for the clinical treatment of renal IR injury.

scholarly journals Isoquercitrin Ameliorates Cisplatin-Induced Nephrotoxicity Via the Inhibition of Apoptosis, Inflammation, and Oxidative Stress

2020 ◽  
Vol 11 ◽  
Author(s):  
Hao Wang ◽  
Weiwei Xia ◽  
Guangfeng Long ◽  
Zhiyin Pei ◽  
Yuanyuan Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Cell Injury ◽  
Kidney Diseases ◽  
Kidney Injury ◽  
Epithelial Cell Line ◽  
Therapeutic Uses ◽  
And Oxidative Stress

Cisplatin is extensively used and is highly effective in clinical oncology; nevertheless, nephrotoxicity has severely limited its widespread utility. Isoquercitrin (IQC), a natural flavonoid widely found in herbage, is well known and recognized for its antioxidant, anti-inflammatory, and anti-apoptotic properties. However, the potential effects and mechanism of IQC in cisplatin-induced acute kidney diseases remain unknown. In this study, we postulated the potential effects and mechanism of IQC upon cisplatin exposure in vivo and in vitro. For the in vivo study, C57BL/6J mice were pretreated with IQC or saline (50 mg/kg/day) by gavage for 3 days before cisplatin single injection (25 mg/kg). Renal function, apoptosis, inflammation, oxidative stress and p-ERK were measured to evaluate kidney injury. In vitro, mouse proximal tubular cells (mPTCs) and human proximal tubule epithelial cell line (HK2) were pretreated with or without IQC (80 μM for mPTCs and 120 μM for HK2) for 2 h and then co-administrated with cisplatin for another 24 h. Apoptosis, inflammation, ROS and p-ERK of cells were also measured. In vivo, IQC administration strikingly reduced cisplatin-induced nephrotoxicity as evidenced by the improvement in renal function (serum creatinine and blood urea nitrogen), kidney histology (PAS staining), apoptotic molecules (cleaved caspase-3, caspase-8, Bax and Bcl-2), inflammatory cytokines (IL-1β, IL-6, TNF-α, and COX-2), oxidative stress (MDA and total glutathione) and p-ERK. In line with in vivo findings, IQC markedly protected against cisplatin-induced cell injury in mPTCs and HK2 cells. Collectively, these findings demonstrated that IQC administration could significantly protect against cisplatin nephrotoxicity possibly through ameliorating apoptosis, inflammation and oxidative stress accompanied by cross talk with p-ERK. Furthermore, IQC may have potential therapeutic uses in the treatment of cisplatin-induced acute kidney injury.

scholarly journals Curcumin Protects against Renal Ischemia/Reperfusion Injury by Regulating Oxidative Stress and Inflammatory Response

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Xiaoying Cui ◽  
Lili Lin ◽  
Xiaoling Sun ◽  
Lin Wang ◽  
Rong Shen
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
Renal Injury ◽  
Iron Reduction ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Renal Ischemia ◽  
Inflammatory Factors ◽  
Left Renal Artery ◽  
Contralateral Kidney

Objective. The aim of this study was to explore the pharmacological effects of curcumin on oxidative stress and inflammatory response of renal dysfunction induced by renal ischemia/reperfusion (RIRI). Methods. Fifty male SD rats (Sprague Dawley) were randomly divided into the sham group, RIRI group, and curcumin group (low, medium, and high). The RIRI model was established by clipping the left renal artery for 45 min and then reperfusion for 24 h and resection of the contralateral kidney. In the curcumin group, curcumin was intraperitoneally injected once a day for 3 consecutive days using different dosage regimens. The RIRI group was intraperitoneally administered with normal saline. Renal injury was evaluated by measuring the concentration of creatinine (Cr) and urea nitrogen (BUN) in serum. Oxidative stress was assessed by assessing the level of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH), and iron reduction/antioxidant capacity (FRAP) in tissues. In addition, the protective effect of RIRI was investigated by measuring Paller scores, the level of serum inflammatory factors and caspase-3, and the number of apoptotic cells. Results. Ischemia/reperfusion resulted in increased levels of Cr and BUN in serum and MDA in tissues and decreased levels of SOD, CAT, GPx, GSH, and FRAP. Curcumin pretreatment strikingly increased the level of SOD, CAT, GPx, GSH, IL-10, IFN-γ, and FRAP and significantly decreased MDA, Cr, BUN, IL-8, TNF-α, IL-6, and myeloperoxidase (MPO) expressions in tissues. Conclusion. Curcumin can relieve the degree of renal injury and improve renal function in ischemia-reperfusion, which may be related to the fact that curcumin can increase SOD content in serum and reduce MDA and FRAP levels in the rat model.

scholarly journals Effect of complete and partial capsulotomy on the renal function tests and oxidative stress markers in rats undergoing ischemia-reperfusion injury

2019 ◽  
Vol 20 (4) ◽  
pp. 29-36
Author(s):  
Yomna Khater ◽  
Awad Rizk ◽  
Mohamed Saad ◽  
Adel Zaghloul
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Serum Creatinine ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Post Surgery ◽  
Renal Function Tests ◽  
And Oxidative Stress

Objective: To compare the effect of complete and partial renal capsulotomy on the renal function tests and oxidative stress markers in rats undergoing ischemia-reperfusion injury. Design: Randomized controlled experimental study. Animals: A 60 Spraque-dawely rats weighing 180 ± 50 g. Procedures: Rats were divided into 3 groups in triplicate (6 each). In addition, 6 rats were subjected to blood and renal tissues sampling for estimation of normal parameters. Group 1 (Positive control): ischemia reperfusion (IR) injury; Group 2: Complete capsulotomy + I R; Group 3: Partial capsulotomy + IR. Six rats from each group were sacrified at 2, 7 and 14 days post- surgery. Results: The complete capsulotomy induced a significant decrease in the serum creatinine at 2 and 7 days post- capsulotomy in comparison with partial capsulotomy (P < 0.05), whereas at 14 days, the partial capsulotomy induced the significant decrease (P < 0.05). Complete capsulotomy showed a significant improvement in creatinine clearance in comparasion with partial capsulotomy at 2, 7 and 14 days post- surgery (p<0.05). At 2 and 7 days, BUN of IR+ Capsulotomy group showed a significant decrease (P < 0.05) compared to the other groups, while at 14 days partial capsulotomy, the serum BUN reached to the normal value. Serum sodium level showed a significant decrease (P < 0.05) at 2 days after partial capsulotomy, and at 14 days after complete capsulotomy (P < 0.05). Nitric oxide level in IR + partial capsulotomy group showed a significant decrease at 7 and 14 days (P < 0.05). Results of MDA of IR+ partial capsulotomy groups showed a significant decrease (P < 0.05) compared to the IR+ compete capsulotomy groups at 2,7 and 14 days. Conclusion and clinical relevance: The partial capsulotomy ameliorates could improve serum creatinine, BUN and could lower the oxidative stress at 14 days. Partial capsulotomy could also improve the renal tissues at both short and long-term. So this study indicates the importance of the presence of intact renal capsule for ischemic acute kidney injury.

scholarly journals In Vivo and In Vitro Evaluation of Urinary Biomarkers in Ischemia/Reperfusion-Induced Kidney Injury

2021 ◽  
Vol 22 (21) ◽  
pp. 11448
Author(s):  
Keiko Hosohata ◽  
Denan Jin ◽  
Shinji Takai
Keyword(s):  
Oxidative Stress ◽  
Ischemia Reperfusion ◽  
Kidney Injury ◽  
Experimental Models ◽  
Left Renal Artery ◽  
Tubular Injury ◽  
Renal Tubular ◽  
High Level

Oxidative stress plays an important role in the pathophysiology of acute kidney injury (AKI). Previously, we reported that vanin-1, which is involved in oxidative stress, is associated with renal tubular injury. This study was aimed to determine whether urinary vanin-1 is a biomarker for the early diagnosis of AKI in two experimental models: in vivo and in vitro. In a rat model of AKI, ischemic AKI was induced in uninephrectomized rats by clamping the left renal artery for 45 min and then reperfusing the kidney. On Day 1 after renal ischemia/reperfusion (I/R), serum creatinine (SCr) in I/R rats was higher than in sham-operated rats, but this did not reach significance. Urinary N-acetyl-β-D-glucosaminidase (NAG) exhibited a significant increase but decreased on Day 2 in I/R rats. In contrast, urinary vanin-1 significantly increased on Day 1 and remained at a significant high level on Day 2 in I/R rats. Renal vanin-1 protein decreased on Days 1 and 3. In line with these findings, immunofluorescence staining demonstrated that vanin-1 was attenuated in the renal proximal tubules of I/R rats. Our in vitro results confirmed that the supernatant from HK-2 cells under hypoxia/reoxygenation included significantly higher levels of vanin-1 as well as KIM-1 and NGAL. In conclusion, our results suggest that urinary vanin-1 might be a potential novel biomarker of AKI induced by I/R.

scholarly journals Nephroprotective effect of methanol extract of Moringa oleifera leaves on acute kidney injury induced by ischemia-reperfusion in rats

2020 ◽  
Vol 20 (3) ◽  
pp. 1382-1396
Author(s):  
Akinleye Stephen Akinrinde ◽  
Olusegun Oduwole ◽  
Fadeyemi Joseph Akinrinmade ◽  
Folusho Bolawaye Bolaji-Alabi
Keyword(s):  
Oxidative Stress ◽  
Moringa Oleifera ◽  
Low Dose ◽  
Methanol Extract ◽  
Ischemia Reperfusion ◽  
Kidney Injury ◽  
Control Group ◽  
Left Renal Artery ◽  
High Dose ◽  
Group B

Background: Moringa oleifera is known to exhibit protection against oxidative damage due to its rich content of compounds with antioxidant activity. This study investigated the protective effect of the methanol extract of Moringa oleifera (MO) in a rat model of renal ischemia-reperfusion (IR) injury. Methods: Forty two wistar rats were randomly assigned to six groups of seven rats each, as follows: A, control group; B, sham-operated group; C, IR group; D, IR + low dose (200 mg/kg) MO; E, IR + high dose (400 mg/kg) MO and F, IR + Vitamin C (200 mg/kg). Unilateral ischaemia was induced by occluding the left renal artery for 45 minutes followed by rep- erfusion up to 24 hours. Results: Moringa oleifera significantly (p<0.05) ameliorated IR-induced increases in malondialdehyde (MDA), protein carbon- yls (PC) and advanced oxidation protein products (AOPP), while also decreasing serum BUN and Creatinine levels. More- over, the low dose of MO caused reductions in renal NO and H2O2 levels, while increasing renal GPx and GST activities. Histopathology revealed marked improvement of tissue alterations induced by IR with both doses of MO. Conclusion: Overall, the methanol extract of M. oleifera effectively attenuated the deleterious effects of renal IR via allevi- ation of tissue oxidative stress. Keywords: Oxidative stress; ischaemia-reperfusion; Moringa oleifera; kidneys; antioxidants.

scholarly journals Polyphenol-rich extract of Ocimum gratissimum leaves Prevented Toxic Effects of Cyclophosphamide on the kidney Function of Wistar rats

2021 ◽  
Author(s):  
Quadri K. Alabi ◽  
Rufus O. Akomolafe ◽  
Joseph G. Akomolafe ◽  
Ayodeji Aturamu ◽  
Mokolade S. Ige ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Low Cost ◽  
Plasma Concentrations ◽  
Kidney Injury ◽  
Ocimum Gratissimum ◽  
Reactive Protein ◽  
Aldosterone Production ◽  
Food And Water Intake ◽  
And Oxidative Stress

Abstract Background Cyclophosphamide (CP) is one of the potent and low cost chemotherapy used in clinical setting against a variety of tumors. However, its association with nephrotoxicity limits its therapeutic use. Ocimum gratissimum leaf is a natural plant with numerous pharmacological and therapeutic efficacies, such as antioxidant, anti-inflammation, and anti-apoptotic properties.Methods The present study was designed to evaluate the protective effect of Ocimum gratissimum (OG) against CP-induced kidney dysfunction in rats. Rats were pre-treated with 400 mg/kg b.w. of polyphenol-rich Ocimum gratissimum leaves (PREOG) for 4 days and then 50 mg/kg b.w. of CP was co-administered from day 5 to day 7 along with PREOG. Markers of renal function and oxidative stress, food and water intake, electrolytes, aldosterone, leukocytes infiltration, inflammation and histopathological alteration were evaluated.Results Obvious renal inflammation and kidney injuries were observed in CP treated groups. PREOG administration prevented oxidative stress, kidney injuries, attenuated inflammation, increased aldosterone production and reduced sodium and water loss in rats. PREOG also decreased the plasma concentrations of Interleukin-(IL)-6, C-reactive protein and activity of myeloperoxidase and malondialdehyde in CP treated rats.Conclusion OG prevented kidney injury and enhanced renal function via inhibiting inflammation and oxidant-induced CP toxicity. The efficacy of OG is related to the presence of various phytochemicals in the plant.

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