Analysis of pH and release of calcium of association between melaleuca alternifolia oil and calcium hydroxide

Abstract Introduction The use of intracanal medications with antimicrobial properties is essential for decontaminating root canals during endodontic treatment. Calcium hydroxide is used for this because of its excellent properties. Melaleuca alternifolia oil has shown medicinal importance by demonstrating antifungal and bactericidal action against proven human pathogens. Objective To evaluate the physical and chemical aspects such as pH and calcium release, of Melaleuca alternifolia oil associated with calcium hydroxide, during different time intervals. Material and method Calcium hydroxide powder was added to vehicles to reach a concentration of 72mg / 0.1mL. Three groups were formed: Group I: Calcium Hydroxide + Distilled Water; Group II: Calcium hydroxide + Propylene Glycol; Group III: Calcium hydroxide + Melaleuca oil. The pH of each group was measured after time intervals of 10 minutes; 24 and 48 hours; 7, 15 and 30 days after tooling by a pH meter. Calcium release was analyzed by atomic absorption spectrometry equipped with a calcium hollow cathode lamp. Data were statistically analyzed by using the Kruskall-Wallis and Dunn test. Result Group II showed high pH, similar to group III that remained uniform at 15 and 30 days. Calcium release that began after 24 hours, was similar in Groups II and III, and showed a peak release in 48 hours. Conclusion The association of Melaleuca oil with calcium hydroxide showed good results in the pH and calcium release analyses, and showed action similar to that of propylene glycol + calcium hydroxide.

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Propolis: A Smart Supplement for an Intracanal Medicament

International Journal of Clinical Pediatric Dentistry ◽

10.5005/jp-journals-10005-1459 ◽

2017 ◽

Vol 10 (4) ◽

pp. 324-329

Author(s):

Alok Avinash ◽

Harsha Munot ◽

Rashmi Baranwal ◽

Vijay Duggi ◽

Alok Dubey ◽

...

Keyword(s):

Calcium Hydroxide ◽

Propylene Glycol ◽

Root Canal ◽

Permanent Teeth ◽

Dentinal Tubules ◽

Group Iii ◽

Ph Values ◽

Group I ◽

The Mean ◽

Group Ii

ABSTRACT Introduction One of the most important factors for successful endodontic therapy is root canal cleaning. The difficulty involved in eliminating microorganisms, as well as their residual presence, warrants the use of root canal dressings after biomechanical preparation. Aim The aim of the study was to compare the diffusion ability between nonalcoholic calcium hydroxide—propolis paste, calcium hydroxide—saline paste, and calcium hydroxide—propylene glycol paste. Materials and methods For this proposed study, single-rooted extracted permanent teeth were randomly divided into three groups to fill the canals: group I: Calcium hydroxide—propylene glycol paste, group II: Calcium hydroxide—saline paste, and group III: Calcium hydroxide—propolis paste. After complete filing of the root canal, the pH values of the solutions in the flasks are measured at an interval of 3, 24, 72, 168 hours. Results After 168-hour interval, it was noticed that the mean pH obtained by calcium hydroxide—propolis paste was 10.54 (± 0.38), which was greater than calcium hydroxide—propylene glycol paste 9.70 (± 0.45) and calcium hydroxide—saline paste 9.16 (± 0.30) consecutively. Conclusion The nonalcoholic calcium hydroxide—propolis paste used during the study was able to diffuse through the dentinal tubules. Thus, it can be used as a vehicle for calcium hydroxide. How to cite this article Baranwal R, Duggi V, Avinash A, Dubey A, Pagaria S, Munot H. Propolis: A Smart Supplement for an Intracanal Medicament. Int J Clin Pediatr Dent 2017;10(4):324-329.

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Assessment of the Antibacterial Activity of Calcium Hydroxide Combined with Chlorhexidine Paste and Other Intracanal Medications against Bacterial Pathogens

European Journal of Dentistry ◽

10.1055/s-0039-1698852 ◽

2011 ◽

Vol 05 (01) ◽

pp. 001-007 ◽

Cited By ~ 8

Author(s):

Cláudia Fernandes de Magalhães Silveira ◽

Rodrigo Sanches Cunha ◽

Carlos Eduardo Fontana ◽

Alexandre Sigrist de Martin ◽

Brenda Paula Figueiredo de Almeida Gomes ◽

...

Keyword(s):

Antibacterial Activity ◽

Calcium Hydroxide ◽

Propylene Glycol ◽

Group Iv ◽

Microbial Cells ◽

Group Iii ◽

Group I ◽

In Vitro Antibacterial Activity ◽

Resistant Microorganism

ABSTRACTObjectives: The purpose of this study was to assess the in vitro antibacterial activity of four formulations of calcium hydroxide [Ca(OH)2] pastes against Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus mutans. Methods: A broth dilution test was performed, and the lengths of time for different pastes to kill the microbial cells were recorded and statistically analyzed. The following medications were assessed: Group I Ca(OH)2 + 2.0% chlorhexidine (CHX) gel; Group II Ca(OH)2 + camphorated paramonochlorophenol (CMCP) and propylene glycol; Group III Ca(OH)2 + propylene glycol; Group IV Ca(OH)2 + saline. Results: The results showed that E. faecalis was the most resistant microorganism. Groups II and III eliminated all the microbial cells in 15 seconds. Group I took 45 seconds to eliminate E. faecalis. Conclusions: Under the conditions of this study, it was concluded that all the intracanal medications tested showed antibacterial activity. However, the association of Ca(OH)2 and PMCC or Ca(OH)2 and propylene glycol showed a better performance, since Groups II and III took a shorter length of time than the other groups to eliminate S. aureus and E. faecalis. (Eur J Dent 2011;5:1-7)

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Effect of 17% EDTA on removal of canal wall smear layer and calcium hydroxide dressing: part II

RGO - Revista Gaúcha de Odontologia ◽

10.1590/1981-8637201400010000082382 ◽

2014 ◽

Vol 62 (1) ◽

pp. 53-58

Author(s):

Rosana de Souza PEREIRA ◽

Gisela de Souza PEREIRA ◽

Juliana Machado BARROSO ◽

Carlos Henrique Siqueira BARROS ◽

Márcia Gabriella Lino de Barros BORTOLOTTI ◽

...

Keyword(s):

Calcium Hydroxide ◽

Propylene Glycol ◽

Root Canal ◽

Smear Layer ◽

Canal Wall ◽

Group Iii ◽

Group I ◽

Working Length ◽

Almost All ◽

Scanning Electron

OBJECTIVE: the aim of this study was to evaluate, by scanning electron microscopy, the effectiveness of 17% EDTA on removal of canal wall smear layer and calcium hydroxide dressing in different thirds of root canal. METHODS: Ttwenty-four premolars were instrumented in the cervical and middle thirds using Gates-Glidden drills. At the working length, the canal was widened up to # 35 file, followed by scaling up to file # 50. After instrumentation, the teeth were divided into four groups according to the treatment received: GI - irrigation performed with 5 ml of NaOCl 2.5 % ; GII - irrigation with 5 ml of 17% EDTA for 2 and a half minutes , submitted to agitation with file # 15, followed by irrigation with 5 ml of 2.5% NaOCl ; GIII - irrigation with 5 ml of NaOCl 2.5% , drying , application of temporary dressing of Ca (OH )2 with propylene glycol and sealing. After 5 days , removing the sealing irrigation with 5 ml of 17% EDTA for 2 and a half minutes , submitted to agitation with file # 15, followed by a final flush with 5 ml of 2.5% NaOCl ; GIV - irrigation with 5 ml of 17% EDTA for 2 and a half minutes , submitted to agitation with file # 15 , followed by irrigation with 5 ml of 2.5% NaOCl , drying and after use of the medication in Ca (OH )2 with the sealing and propylene glycol which was removed after 5 days performing the irrigation with 5 ml of 17% EDTA for 2 and a half minutes , submitted to agitation with file # 15, followed by using 5 ml 2.5% NaOCl . Next, the teeth were prepared and taken to the scanning electron microscope . RESULTS: The images revealed: Group I - a marked presence of smear layer in all three thirds, Group II - removal of almost all of the smear layer in the cervical third and removal of large quantity in the middle third, whereas in the apical third a large quantity of smear layer still remained however, in comparison with the other groups, this was shown to be cleaner; Group III - in the three thirds, the presence of smear layer was observed, which was shown to be much less compacted than that observed in Group 1; Group IV- in all the thirds there was reduction of smear layer and Ca(OH)2-based medication, and presence of several unobstructed dentinal tubules could be observed, demonstrating the second best result observed. CONCLUSION: It could be concluded that the use of 17% EDTA favored removal of the smear layer and intracanal medication residues in all thirds of the root canal.

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Proliferation of odontoblast-like cells following application of a combination of calcium hydroxide and propolis

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v52.i4.p183-186 ◽

2019 ◽

Vol 52 (4) ◽

pp. 183

Author(s):

Ira Widjiastuti ◽

Sri Kunarti ◽

Fauziah Diajeng Retnaningsih ◽

Evri Kusumah Ningtyas ◽

Debby Fauziah Suryani ◽

...

Keyword(s):

Calcium Hydroxide ◽

Wistar Rats ◽

Control Group ◽

Group V ◽

Propolis Extract ◽

Group Iii ◽

Group I ◽

Occlusal Surfaces ◽

Significant Difference ◽

Group Ii

Background: One purpose of operative dentistry is the maintenance of healthy pulp by reducing the need for root canal treatment and the possibility of undesirable scenarios such as tooth loss. Propolis is a plant-derived substance that contains a resin produced by honeybees belonging to the Apis mellifera species. Purpose: This study aimed to investigate the effect of a combination of calcium hydroxide (Ca(OH)2) and propolis extract on odontoblast-like cell proliferation in Wistar rats (Rattus norvegicus). Methods: This research constituted a true experimental laboratory-based investigation with post-test control group design. Thirty Wistar rats were randomly divided into six groups. The first molar pulp of each sample was perforated on occlusal surfaces using a low speed round bur. On day 3, the samples were divided into six groups (n=10): Group I: control; Group II: Ca(OH)2 + 11%; propolis extract; Group III: Ca(OH)2 + aquadest, and on day 7: Group IV: control; Group V: Ca(OH)2 + 11% propolis extract; Group VI: Ca(OH)2 + aquadest. All samples were filled with restorative material. They were subsequently sacrificed after 3 and 7 days post-pulp capping administration and the afflicted tooth extracted for hematoxylin and eosin (H&E) staining. The resulting data was subjected to statistical analysis to ascertain the proliferation of odontoblast-like cells. The significance of differences between the groups was determined by a one-way ANOVA test followed by a post hoc Tuckey HSD. A p-value <0.05 was considered to be significant. Results: On day 3, a significant difference existed between group II (Ca(OH)2–propolis) and group I (control group) and group III (Ca(OH)2–aquades), whereas Ca(OH)2–propolis revealed that the proliferation of odontoblast-like cells was higher. Meanwhile, on day 7, there was a significant difference between all groups whereas, with regard to Ca(OH)2–propolis, the proliferation of odontoblast-like cells in group V was higher. Conclusion: Application of combination of Ca(OH)2-propolis extract can increase the proliferation of odontoblast-like cells in pulp tissue on days 3 and 7.

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Antimicrobial activity and biocompatibility of a calcium hydroxide - and aloe vera-based intracanal medication

Revista Brasileira de Odontologia ◽

10.18363/rbo.v74n3.p.180 ◽

2017 ◽

Vol 74 (3) ◽

pp. 180

Author(s):

Liza Porcaro Bretas ◽

Carolina Oliveira de Lima ◽

Nadia Rezende Barbosa Raposo ◽

Beatriz Julião Vieira Aarestrup ◽

Maíra Do Prado ◽

...

Keyword(s):

Antimicrobial Activity ◽

Calcium Hydroxide ◽

Propylene Glycol ◽

Subcutaneous Tissue ◽

Aloe Vera ◽

Control Group ◽

Group Iii ◽

The Third ◽

Histological Characteristics ◽

Group Ii

Objective: to investigate the antimicrobial activity and biocompatibility of the calcium hydroxide [Ca(OH)2] + propylene glycol (PEG) + aloe vera (AV) paste in comparison with other pastes used as intracanal medication. Material and Methods: there was evaluated 3 intracanal medication based on calcium hydroxide and propylene glycol, varying only the third component. In group 1, the third component was camphorated paramonoclorofenol (PMCC); in group II, chlorhexidine (CLX); and in group III, aloe vera. The antimicrobial activity was analyzed through the direct contact of the intracanal medication pastes with strains of Enterococcus faecalis; Kocuria rhizophila; Pseudomonas aeruginosa and Candida albicans. The biocompatibility was evaluated in subcutaneous tissue of rats during experimental 7, 21 and 63 days. Results: it was observed that the Group II showed the best results regarding antimicrobial activity, followed by group III and I. The Ca(OH)2 + PEG + AV paste was considered biocompatible since it presented discrete fibrosis and suggestive histological characteristics of normal healing after 63 days, approaching the control group. Conclusion: the association of Ca (OH)2 + PEG + AV showed antibacterial activity and adequate biocompatibility when compared with commonly pastes used as intracanal medication.

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Content of Glutathione and Vitamin C in the Liver of Rats Exposed to Dimethoate and Pyrantel Tartrate

Acta Veterinaria Brno ◽

10.2754/avb200877030355 ◽

2008 ◽

Vol 77 (3) ◽

pp. 355-362 ◽

Cited By ~ 3

Author(s):

A. Spodniewska ◽

A. Zasadowski

Keyword(s):

Vitamin C ◽

Oral Administration ◽

Rat Liver ◽

Time Intervals ◽

Group Iii ◽

Group I ◽

Liver Homogenates ◽

Group Ii ◽

Pyrantel Tartrate

The study was undertaken to examine the effect of oral administration of dimethoate (Bi 58 Nowy) and/or pyrantel tartrate on the concentration of glutathione (GSH) and vitamin C in rat liver. Rats of Group I were administered pyrantel tartrate at a dose of 85 mg/kg b.w. at a twoweek interval, while the animals of Group II received Bi 58 Nowy (38% dimethoate) at a dose of 25 mg/kg b.w. for 28 days, and animals of Group III received both compounds together as described above. Pyrantel tartrate was found to increase the concentration of glutathione in the liver, whereas the content of vitamin C oscillated around values reported for the control. After a 28-day exposure to dimethoate (Bi 58 Nowy), except for the 6th hour after intoxication, the content of GSH was observed to increase and significant differences (p ≤ 0.05) occurred after days 3 and 14 (p ≤ 0.01). The concentration of vitamin C in liver homogenates after dimethoate administration was decreased compared to the control until day 3. An increase was then observed continuing until the end of the experiment. In animals receiving both compounds, except for day 7, the concentration of vitamin C was slightly decreased. The administration of pyrantel tartrate before dimethoate (Bi 58 Nowy) only in some time intervals was found to reduce the intensity of changes evoked by the exclusive administration of insecticide. It may suggest that not in all cases of mixed intoxications, intensification should be expected in changes of the variables analyzed.

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Evaluation of the Efficacy of Coenzyme Q10 in the Management of Chronic Periodontitis: A Clinical Study

Dental Journal of Advance Studies ◽

10.1055/s-0041-1736267 ◽

2021 ◽

Author(s):

Gaurav Pandav ◽

Sakshi Pandav ◽

Sanjeev Jain ◽

Divya Saxena ◽

Ridhi Aggarwal ◽

...

Keyword(s):

Coenzyme Q10 ◽

Chronic Periodontitis ◽

Time Intervals ◽

Pocket Depth ◽

Group Iii ◽

Probing Pocket Depth ◽

Group I ◽

Intergroup Comparison ◽

Significant Difference ◽

Group Ii

Abstract Aim The present study was aimed to clinically evaluate the effectiveness of coenzyme Q10 (CoQ10) in the management of chronic periodontitis. Materials and Methods A total of 60 patients aged between 30 and 60 years with bleeding on probing and probing pocket depth (PPD) of 3 to 5 mm were selected and divided into three groups, with group I receiving scaling and root planing, group II CoQ10 formulation for 6 weeks, and group III receiving both scaling and root planning, followed by coenzyme Q10 administration for 6 weeks. PPD, relative attachment level (RAL), and gingival index were recorded in all the groups at baseline, 6 weeks, and 3 months, respectively. The data was statistically analyzed using Kruskal–Wallis, Mann–Whitney, and Wilcoxon signed rank tests. Result Intragroup comparison showed statistically significant difference (p ≤ 0.05) between the clinical parameters of all the groups at all time intervals, whereas intergroup comparison of all the parameters showed high statistically significant difference (p ≤ 0.001) in group III at various time intervals followed by group I and group II. Conclusion It was concluded from the study that CoQ10 is a useful adjunct in treating chronic periodontitis by boosting the host resistance to periodontal disease.

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Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068035 ◽

1970 ◽

Vol 28 ◽

pp. 208-209

Author(s):

K.K. SEKHRI ◽

C.S. ALEXANDER ◽

H.T. NAGASAWA

Keyword(s):

Osmium Tetroxide ◽

Male Mice ◽

Alcohol Group ◽

Group Iii ◽

Group I ◽

C57bl Mice ◽

Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

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Heterogeneity and Immunochemical Properties of Anti-β2-glycoprotein I Autoantibodies

Thrombosis and Haemostasis ◽

10.1055/s-0037-1615218 ◽

1998 ◽

Vol 80 (09) ◽

pp. 393-398 ◽

Cited By ~ 33

Author(s):

V. Regnault ◽

E. Hachulla ◽

L. Darnige ◽

B. Roussel ◽

J. C. Bensa ◽

...

Keyword(s):

Fluid Phase ◽

Anticardiolipin Antibodies ◽

Dual Reactivity ◽

Group Iii ◽

Important Group ◽

Epitope Specificity ◽

Glycoprotein I ◽

Group I ◽

Group Ii ◽

Sufficient Concentration

SummaryMost anticardiolipin antibodies (ACA) associated with antiphospholipid syndrome (APS) are directed against epitopes expressed on β2-glycoprotein I (β2GPI). Despite a good correlation between standard ACA assays and those using purified human β2GPI as the sole antigen, some sera from APS patients only react in the latter. This is indicative of heterogeneity in anti-β2GPI antibodies. To characterize their reactivity profiles, human and bovine β2GPI were immobilized on γ-irradiated plates (β2GPI-ELISA), plain polystyrene precoated with increasing cardiolipin concentrations (CL/β2GPI-ELISA), and affinity columns. Fluid-phase inhibition experiments were also carried out with both proteins. Of 56 selected sera, restricted recognition of bovine or human β2GPI occurred respectively in 10/29 IgA-positive and 9/22 IgM-positive samples, and most of the latter (8/9) were missed by the standard ACA assay, as expected from a previous study. Based on species specificity and ACA results, IgG-positive samples (53/56) were categorized into three groups: antibodies reactive to bovine β2GPI only (group I) or to bovine and human β2GPI, group II being ACA-negative, and group III being ACA-positive. The most important group, group III (n = 33) was characterized by (i) binding when β2GPI was immobilized on γ-irradiated polystyrene or cardiolipin at sufficient concentration (regardless of β2GPI density, as assessed using 125I-β2GPI); (ii) and low avidity binding to fluid-phase β2GPI (Kd in the range 10–5 M). In contrast, all six group II samples showed (i) ability to bind human and bovine β2GPI immobilized on non-irradiated plates; (ii) concentration-dependent blockade of binding by cardiolipin, suggesting epitope location in the vicinity of the phospholipid binding site on native β2GPI; (iii) and relative avidities approximately 100-fold higher than in group III. Group I patients were heterogeneous with respect to CL/β2GPI-ELISA and ACA results (6/14 scored negative), possibly reflecting antibody differences in terms of avidity and epitope specificity. Affinity fractionation of 23 sera showed the existence, in individual patients, of various combinations of antibody subsets solely reactive to human or bovine β2GPI, together with cross-species reactive subsets present in all samples with dual reactivity namely groups III and II, although the latter antibodies were poorly purified on either column. Therefore, the mode of presentation of β2GPI greatly influences its recognition by anti-β2GPI antibodies with marked inter-individual heterogeneity, in relation to ACA quantitation and, possibly, disease presentation and pathogenesis.

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Oxygen Fraction Adjustment According to Body Surface Area during Extracorporeal Circulation

The Heart Surgery Forum ◽

10.1532/hsf.1325 ◽

2015 ◽

Vol 18 (3) ◽

pp. 098

Author(s):

Cem Arıtürk ◽

Serpil Ustalar Özgen ◽

Behiç Danışan ◽

Hasan Karabulut ◽

Fevzi Toraman

Keyword(s):

Body Temperature ◽

Surface Area ◽

Body Surface Area ◽

Body Surface ◽

Extracorporeal Circulation ◽

Group Iii ◽

Oxygen Fraction ◽

Arterial Blood ◽

Group I ◽

Group Ii

Background: The inspiratory oxygen fraction (FiO2) is usually set between 60% and 100% during conventional extracorporeal circulation (ECC). However, this strategy causes partial oxygen pressure (PaO2) to reach hyperoxemic levels (>180 mmHg). During anesthetic management of cardiothoracic surgery it is important to keep PaO2 levels between 80-180 mmHg. The aim of this study was to assess whether adjusting FiO2 levels in accordance with body temperature and body surface area (BSA) during ECC is an effective method for maintaining normoxemic PaO2 during cardiac surgery.Methods: After approval from the Ethics Committee of the University of Acıbadem, informed consent was given from 60 patients. FiO2 adjustment strategies applied to the patients in the groups were as follows: FiO2 levels were set as 0.21 × BSA during hypothermia and 0.21 × BSA + 10 during rewarming in Group I; 0.18 × BSA during hypothermia and 0.18 × BSA + 15 during rewarming in Group II; and 0.18 × BSA during hypothermia and variable with body temperature during rewarming in Group III. Arterial blood gas values and hemodynamic parameters were recorded before ECC (T1); at the 10th minute of cross clamp (T2); when the esophageal temperature (OT) reached 34°C (T3); when OT reached 36°C (T4); and just before the cessation of ECC (T5).Results: Mean PaO2 was significantly higher in Group I than in Group II at T2 and T3 (P = .0001 and P = .0001, respectively); in Group I than in Group III at T1 (P = .02); and in Group II than in Group III at T2, T3, and T4 (P = .0001 for all). Conclusion: Adjustment of FiO2 according to BSA rather than keeping it at a constant level is more appropriate for keeping PaO2 between safe level limits. However, since oxygen consumption of cells vary with body temperature, it would be appropriate to set FiO2 levels in concordance with the body temperature in the rewarming period.

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