Treatment of Solanum torvum seeds improves germination in a batch-dependent manner1

ABSTRACT The Solanum torvum species can grow in soils with a heavy load of nematodes and pathogenic fungi. It is currently much in demand in intensive agriculture as a rootstock of Solanaceae species, such as eggplant and tomato. This study aimed at comparing treatments, in order to determine the best method to accelerate the germination of S. torvum seed batches. Three seed batches were submitted to four treatments to overcome dormancy (water, potassium nitrate, gibberellic acid and pre-imbibition in gibberellic acid). The first germination count, germination percentage, germination speed index, mean germination time and mean germination speed were assessed. Treatments with gibberellic acid, with either pre-imbibition or only moistened substrate, exhibited the best germination speed index, mean germination time and mean germination speed. The final germination percentage showed a significant interaction between treatments and seed batches. Therefore, the treatments affect the final germination in a batch-dependent manner.

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Pre-germinative treatments and seed storage of Psidium cattleyanum Sabine morphotypes

Colloquium Agrariae ◽

10.5747/ca.2020.v16.n4.a387 ◽

2020 ◽

Vol 16 (4) ◽

pp. 102-109

Author(s):

Haron Victor Ferreira Camargo ◽

Letícia Siqueira Walter ◽

Mônica Moreno Gabira ◽

Dagma Kratz

Keyword(s):

Storage Period ◽

Seed Storage ◽

Germination Percentage ◽

Transparent Plastic ◽

Germination Time ◽

Hand Control ◽

Germination Speed ◽

Mean Germination Time ◽

Cold Chamber

This study aimed to evaluate the germination behavior of two morphotypes of P. cattleyanumsubmitted to storage and pre-germinative treatments. The experiment was designed in a factorial scheme 2 x 3 x 3 (morphotypes x storage periods x pre-germinative treatments) completely randomized, using for each treatment 5 replicates with 30 seeds. The seeds were stored in semipermeable packages in a cold chamber at 5 ± 2 °C, for 14, 45,and 90 days. After each storage period, the seeds were submitted to treatments to overcome dormancy: immersion in water at 80 °C,kept in imbibition for 24 and 48 hand control, without any treatment. The seeds were arranged under moistened substrate in transparent plastic boxes and allocated in a germination chamber at 25 °C and photoperiod of 8 h for evaluation of germination percentage (G), germination speed index (GSI)and mean germination time (MGT). The red morphotype seeds showed higher germination percentages than the yellow morphotype and did not have a reduction in these values after 90 days of storage. MGT for yellow morphotype was lower in all pre-germinativetreatments. The GSI had better results at 45 days in the control treatments and 24 h in immersion in water and 90 days for the three pre-germinative treatments. The red morphotype does not require pre-germinative treatments, while the yellow morphotype presents better germination when the seeds are stored for 45 days and immersed in water at 80 °C for 48 h.

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Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

Agronomía Colombiana ◽

10.15446/agron.colomb.v33n3.53074 ◽

2016 ◽

Vol 34 (1) ◽

pp. 17-24 ◽

Cited By ~ 2

Author(s):

Fabio Ernesto Martínez M. ◽

Diego Miranda L. ◽

Stanislav Magnitskiy

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Positive Response ◽

Germination Rate ◽

Germination Percentage ◽

Annona Squamosa ◽

Germination Time ◽

Mean Germination Time ◽

Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

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Breaking seed dormancy of Tulipa scardica Bornm. and Tulipa kosovarica Kit Tan, Shuka & Krasniqi by pre-chilling, plant growth regulators and some chemical treatments

Acta agriculturae Slovenica ◽

10.14720/aas.2019.113.2.1 ◽

2019 ◽

Vol 113 (2) ◽

pp. 203 ◽

Cited By ~ 1

Author(s):

Bekim GASHI ◽

Mirsade OSMANI ◽

Sali ALIU

Keyword(s):

Sulfuric Acid ◽

Plant Growth ◽

Gibberellic Acid ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Potassium Nitrate ◽

Germination Percentage ◽

Dormancy Breaking ◽

Germination Time ◽

Mean Germination Time

Tulipa scardica and T. kosovarica are rare, endemic and endangered plants in Kosovo. This research was carried out to study the dormancy breaking treatment in Tulipa scardica and T. kosovarica seeds by pre-chilling and various concentration of potassium nitrate (KNO3), gibberellic acid (GA3), kinetin, thiourea and sulfuric acid (H2SO4). The experiment was conducted with untreated seeds (without stratification) and with stratified seeds (8 weeks) and treated with different concentration of some chemicals and plant growth regulators. Results showed that the final germination percentage (FGP) of seeds without stratification at both Tulipa plant species was zero, while germination was enhanced by pre-chilling (stratification) especially after treatment of these seeds by above mentioned treatments. Both Tulipa species showed an increase in mean germination time (MGT), an indication of slower germination, as different chemicals or plant growth regulators increased. Depending on treatments, germination was ranging from 80 % to 90 %, the maximum germination was detected in seeds treated with KNO3, GA3, and their combination, while the minimum germination in seeds without treatments and treated with thiourea. The conservation of these plants in botanic gardens by cultivation or propagation for commercial use as ornamental plants could give an effective contribution to the conservation of these plants. Abbreviations: KNO3 – potassium nitrate; GA3 - gibberellic acid; H2SO4 – sulfuric acid; FGP – final germination percentage; MGT – mean germination time

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Dormancy break in seeds of "quina" (Strychnos pseudoquina A. St.-Hil.)

Revista Brasileira de Plantas Medicinais ◽

10.1590/s1516-05722011000400017 ◽

2011 ◽

Vol 13 (4) ◽

pp. 507-511 ◽

Cited By ~ 1

Author(s):

J.M. Vasconcelos ◽

M.A. Rodrigues ◽

S.C. Vasconcelos Filho ◽

J.F. Sales ◽

F.G. Silva ◽

...

Keyword(s):

Sulfuric Acid ◽

Medicinal Plant ◽

Gibberellic Acid ◽

Plant Species ◽

Germination Percentage ◽

Boiling Water ◽

Medicinal Plant Species ◽

Distilled Water ◽

Brazilian Cerrado ◽

Germination Speed

"Quina" (Strychnos pseudoquina A. St. Hil) is a medicinal plant species from the Brazilian Cerrado. As its seeds show dormancy, they were subjected to the treatments pre-cooling at 5ºC during 7 days, pre-heating at 40ºC during 7 days, pre-soaking in sulfuric acid PA during 5 and 15 min, pre-soaking in boiling water during 5 and 15 min, pre-soaking in 100 and 200 ppm gibberellic acid during 48 h, pre-soaking in distilled water during 24 and 48 h, and mechanical scarification to break dormancy. Counts were daily conducted from the 2nd day after the experiment implementation until the germination stabilization at the 65th day. The germination speed index (GSI) and the germination percentage were evaluated. Germination rates above 96% were reached in seeds pre-soaked in water during 48 h and substrate moistened with water or KNO3.

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Physiological potential of stylosanthes cv. Campo Grande seeds coated with different materials

Journal of Seed Science ◽

10.1590/2317-1545v37n2145982 ◽

2015 ◽

Vol 37 (2) ◽

pp. 117-124 ◽

Cited By ~ 6

Author(s):

Priscilla Brites Xavier ◽

Henrique Duarte Vieira ◽

Cynthia Pires Guimarães

Keyword(s):

Activated Carbon ◽

Calcium Silicate ◽

Dry Matter ◽

Seed Quality ◽

Maximum Diameter ◽

Minimum Diameter ◽

Germination Time ◽

Germination Speed ◽

Mean Germination Time ◽

Limestone Sand

The aim of this study was to assess the effect of different coatings on the physiological potential of stylosanthes cv. Campo Grande seeds. The treatments were: uncoated seeds; limestone + PVA glue; limestone + sand + PVA glue; limestone + activated carbon + PVA glue; calcium silicate + PVA glue; calcium silicate + sand + PVA glue; calcium silicate + activated carbon + PVA glue. Posteriorly, the seeds were analyzed for water content (WC), maximum diameter (MAD) and minimum diameter (MID), thousand seed weight (TSW), germination test, germination speed index (GSI), mean germination time (MGT), emergence, emergence speed index (ESI), mean emergence time (MET), shoot and root length, fresh and dry matter of shoot and root. The coating increased the TSW, MAD and MID and decreased its WC. The treatments comprising limestone + PVA glue and limestone + sand + PVA glue increased the germination time, but none of the treatments negatively affected the physiological seed quality. Treatment with calcium silicate + PVA glue was outstanding for germination speed index and fresh and dry matter of shoot and root in the stylosanthes cv. Campo Grande seeds coating.

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Can Bulk and Nanosized Titanium Dioxide Particles Improve Seed Germination Features of Wheatgrass (Agropyron desertorum)

Notulae Scientia Biologicae ◽

10.15835/nsb539072 ◽

2013 ◽

Vol 5 (3) ◽

pp. 325-331 ◽

Cited By ~ 13

Author(s):

Reyhaneh AZIMI ◽

Hassan FEIZI ◽

Mohammad KHAJEH HOSSEINI

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Germination Time ◽

Positive Effects ◽

Tio2 Particles ◽

Nanosized Titanium Dioxide ◽

Mean Germination Time ◽

Germination Value ◽

Improve Seed Germination ◽

Nanosized Tio2

The goal of this study was to evaluate concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L-1 with bulk TiO2 for possible stimulatory effects on wheatgrass seed germination and early growth stage. After 14 days of seed incubation, germination percentage improved by 9% following exposure to 5 ppm nanosized TiO2 treatment comparing to control. Similar positive effects occurred in terms of germination value and mean daily germination. Application of bulk TiO2 particles in 80 ppm concentration greatly decreased the majority of studied traits. Therefore phytotoxicity effect observed on wheatgrass seedling by application of bulk TiO2 particles in 80 ppm concentration. Exposure of wheatgrass seeds to 5 ppm nanosized TiO2 and bulk and nanosized TiO2 at 60 ppm obtained the lowest mean germination time but higher concentrations did not improve mean germination time. In general, there was a positive response by wheatgrass seed to some concentrations of nanosized TiO2. Usage of nanoparicles in order to improve germination and establishment of range plant in adverse environments similar to rangeland could be possible.

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SUPERAÇÃO DA DORMÊNCIA EM SEMENTES DE Plathymenia reticulata Benth.

Nativa ◽

10.31413/nativa.v7i3.6679 ◽

2019 ◽

Vol 7 (3) ◽

pp. 317

Author(s):

Cássio Do Prado Borges ◽

Cheila Deisy Ferreira ◽

Daniela Pereira Dias

Keyword(s):

Electrical Conductivity ◽

Germination Percentage ◽

Lateral Side ◽

Detergent Solution ◽

Sodium Hypochlorite Solution ◽

Percentage Germination ◽

Dormancy Breaking ◽

Germination Speed ◽

Mean Germination Time ◽

T1 And T2

Este trabalho objetivou avaliar a germinação e vigor de sementes de Plathymenia reticulata Benth. submetidas a diferentes tratamentos para superação de dormência. Os tratamentos testados foram: controle - sementes intactas (T0), escarificação mecânica com lixa d’água nº 60 (T1), desponte na região lateral do tegumento (T2), imersão em água fervente por 10 min (T3), em hipoclorito de sódio por 15 min (T4) e em solução com detergente por 15 min (T5). Foram determinados: peso de mil sementes, grau de umidade, porcentagem de germinação, índice de velocidade de germinação (IVG), tempo médio de germinação (TMG), curva de embebição, teste da condutividade elétrica e comprimento da radícula. A porcentagem de germinação das sementes submetidas ao T1 e T2 foi estatisticamente igual (respectivamente, 90 e 88%) e superiores aos outros tratamentos testados (12%). As sementes expostas aos T1 e T2 apesentaram porcentagem de absorção de água superiores e em menor tempo, assim como maiores IVG e menores TMG, comparadas aos demais tratamentos. O comprimento das radículas não variou entre os tratamentos testados. A condutividade elétrica da solução com as sementes do T0 foi superior aos outros tratamentos testados. Sementes de P. reticulata expostas ao T1 e T2 apresentam maior germinação e vigor.Palavras-chave: germinação, índice de velocidade de germinação, teste de condutividade elétrica, vigor, vinhático. DORMANCY BREAKING OF Plathymenia reticulata Benth. SEEDS ABSTRACT:This study aimed to evaluate the germination and vigor of Plathymenia reticulata Benth. seeds submitted to different dormancy breaking treatments. Testing treatments were: control - intact seeds (T0); mechanical scarification with sandpaper n. 60 (T1), coating cutting in the lateral side of tegument (T2), immersion in boiling water during 10 min (T3), in sodium hypochlorite solution during 15 min (T4), and in detergent solution during 15 min (T5). Moisture content, 1000-seed weight, germination percentage, germination speed index (GSI), mean germination time (MGT), imbibition curve, electrical conductivity test, and radicle length were determined. Germination percentage to T1 and T2 were statistically equal (90 and 88 %, respectively) and higher than others treatments tested (12%). Seeds exposed to T1 and T2 also presented higher and faster water absorption percentage, when compared to the others treatments, as well as higher GSI, and lower MGT. The rootlets length did not vary among treatments tested. Electrical conductivity of intact seeds was higher than others treatments. P. reticulata seeds expose to T1 e T2 had highest germination percentage and vigor.Keywords: germination, germination speed index, electrical conductivity test, vigor, vinhático.

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Sensitivity of Seed Germination to Salt Stress in Teff [Eragrostis tef (Zucc.) Trotter]

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:2019.0004 ◽

2019 ◽

Vol 76 (1) ◽

pp. 91

Author(s):

Panayiota PAPASTYLIANOU ◽

Ilias TRAVLOS ◽

Ioannis ROUSSIS ◽

Dimitrios BILALIS

Keyword(s):

Salt Stress ◽

Agricultural Development ◽

Animal Feed ◽

Moisture Stress ◽

Germination Percentage ◽

Limiting Factors ◽

Eragrostis Tef ◽

Germination Time ◽

Randomized Design ◽

Mean Germination Time

Teff is an annual grass originated in Ethiopia, grown for both human food and animal feed. Salinity and moisture stress are the main limiting factors of agricultural development on arid and semi-arid regions. The aim of this study was to investigate the effect of salinity due to NaCl on germination of teff. A set of experiments was conducted under a completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 25 °C) in the dark for 10 days. In order to create salt stress conditions during the germination process, seven solutions were used (0 as control, 80, 160, 240 and 320, 400 and 480 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The presence of NaCl reduced germination, especially above 240 mM for 15 °C and 400 mM for 25 °C. The mean germination time increased with higher NaCl levels.

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Sample size for determination of the physiological potential of coriander (Coriandrum sativum L.) seeds

Australian Journal of Crop Science ◽

10.21475/ajcs.19.13.04.p1482 ◽

2019 ◽

Vol 13 ((04) 2019) ◽

pp. 552-557

Author(s):

Érica Fernandes Leão Araújo ◽

Anderson Rodrigo da Silva ◽

Amanda Rithieli Pereira dos Santos ◽

Kaique Ferreira Alves, Lara Bernardes da S. Ferreira ◽

Hygor Amaral Santana ◽

...

Keyword(s):

Sample Size ◽

Reference Sample ◽

Seed Vigor ◽

Species Determination ◽

Germination Time ◽

Germination Speed ◽

Minimum Number ◽

Mean Germination Time ◽

Number Of Seeds

The objective of this work was to determine the minimum number of replications and seeds per replication to perform the germination and seed vigor tests with coriander seeds. Two seed lots were compared in terms of water content, vigor and viability. Eight hundred seeds per lot were used. Values of germination first count, germination, germination speed index and mean germination time were analyzed. Sample size scenarios were developed using different combinations of number of replications (from 2 to 10) and the number of seeds per replication (from 20 to 80) by means of a resampling with replacement technique. The reference sample consisted of four replications of 50 seeds, as it is commonly used in researches with this species. Determination of the minimum number of replications and seeds was based on comparing the 95% bootstrapped confidence intervals for the index of variation (CV/n0.5) of each scenario with the confidence interval of the reference sample. It is reasonable to reduce the number of seeds per replication from 50 to 30 in order determine germination first count, germination and germination speed index. Forty seeds per replication are recommended to quantify the mean germination time. However, reductions in the number of replications can affect negatively the accuracy of germination and vigor tests.

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GIBBERELLIC ACID PRIMING ENHANCES MAIZE SEED GERMINATION UNDER LOW WATER POTENTIAL

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v22n1.2021.p17-26 ◽

2021 ◽

Vol 22 (1) ◽

pp. 17

Author(s):

Amit Gnawali ◽

Roshan Subedi

Keyword(s):

Gibberellic Acid ◽

Water Potential ◽

Low Cost ◽

Shoot Length ◽

Maize Seedlings ◽

Germination Time ◽

Stressful Environment ◽

Mean Germination Time ◽

Low Water Potential ◽

Germination Parameters

Germination is a portentous yield determining factor that is a challenge in the low water potential environment due to disrupted imbibition. Hormonal seed priming can markedly increase the germination on maize even in such a stressful environment. Therefore, research was aimed to analyze the application of gibberellic acid (GA3) priming to maize seeds to minimize the deleterious effects of reduced water potential. GA3 priming was done at 0, 50, 100, 150, and 200 ppm for 12 hours and subjected to drought levels of 0, 0.15, 0.50, 1.05, and 1.75 MPa by using NaCl solution. Different germination parameters, i.e., germination percentage, mean germination time, germination index, relative water content, seedling vigor index, root length, and shoot length, were determined on the final day of the experiment. The results showed that all germination parameters were adversely influenced by low water potential. Every level of GA3 priming has hastened all parameters. GA3 priming at 100 ppm decreased the mean germination time by 35 hours under 1.75 MPa compared to non-primed seeds. GA3 priming increased the shoot length of maize seedlings with a shoot length of 2.9 cm in non-primed seedlings compared to 6.4 cm in 200 ppm GA3 priming under 1.05 MPa. GA3 priming is the best method for the early establishment of maize seedlings in low water potential conditions. As a result, it may be utilized as a low-cost and straightforward approach for establishing maize crops under saline and drought conditions.

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