scholarly journals Chemical composition, botanical evaluation and screening of radical scavenging activity of collected pollen by the stingless bees Melipona rufiventris (Uruçu-amarela)

2009 ◽  
Vol 81 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Tania M.S. Silva ◽  
Celso A. Camara ◽  
Antonio C.S. Lins ◽  
Maria de Fátima Agra ◽  
Eva M.S. Silva ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Stingless Bees ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Northeastern Brazil ◽  
Scavenging Activity ◽  
Dominant Type ◽  
Etoac Extract ◽  
Pollen Loads ◽  
Pollen Types

Stingless bees in Brazil are indigenous and found all over the country. Bee pollen is used for its nutritional value in the human diet. It is made up of natural flower pollen mixed with nectar and bee secretions. In order to evaluate the chemical composition, free radical scavenging activity, and botanical origin, sample of pollen loads from stingless bee, Melipona rufiventris (Uruçu amarela) was studied. The EtOAc extract of pollen of Melipona rufiventris yielded the following compounds: p-hydroxycinnamic acid, dihydroquercetin, isorhamnetin, isorhamnetin3-O-(6"-O-E-p-coumaroyl)-β-D-glucopyranoside, luteolin, and quercetin. This is the first report of the isolation of isorhamnetin3-O-(6"O-E-p-coumaroyl)β-D-glucopyranoside from pollen. The free radicalscavenging activities of different solvent extracts of pollen were determined using DPPH assay. This activity decreases in the order: EtOAc>EtOH>Hexane extract. It appears that the EtOAc extract of the pollen is a good scavenger of active oxygen species. The botanical evaluation of pollen loads showed the composition by two pollen types, with the dominant type (97.3%) being Scopariadulcis (L.) (Scrophulariaceae) and the minor one Senna obtusifolia (L.) Irwin & Barneby (Fabaceae). This suggests a specific foraging behavior in Melipona rufiventris bees, even in an environment with such a rich botanical diversity as the Northeastern Brazil.

Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke

2006 ◽  
Vol 19 (6-7) ◽  
pp. 507-511 ◽  
Author(s):  
Tania Maria Sarmento Silva ◽  
Celso Amorim Camara ◽  
Antonio Claudio da Silva Lins ◽  
José Maria Barbosa-Filho ◽  
Eva Mônica Sarmento da Silva ◽  
...  
Keyword(s):  
Free Radical ◽  
Chemical Composition ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Stingless Bee ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Pollen Loads ◽  
Melipona Subnitida

scholarly journals Mangifera indica Bark Essential Oil: Chemical Composition and Biological Activities in Comparison with Aqueous and Ethanol Extracts

2018 ◽  
Vol 13 (7) ◽  
pp. 1934578X1801300
Author(s):  
Gislaine Aurelie Kemegne ◽  
Maximilienne Ascension Nyegue ◽  
Sylvain Leroy Sado Kamdem ◽  
François-Xavier Etoa ◽  
Chantal Menut
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Mangifera Indica ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Dpph Method ◽  
Ethanol Extracts

Ethnobotanic surveys have revealed the use of Mangifera indica L. (Anacardiaceae) bark for the treatment of infectious diarrhea. The essential oil of M. indica bark is described for the first time for its chemical composition, radical scavenging activity (DPPH method) and antimicrobial properties. The total phenols content of its water and ethanol bark extracts as well as their radical scavenging and antimicrobial properties were also evaluated. Four commercial plant extracts were also studied for a comparison purpose. The antimicrobial activities were measured for all samples against three Gram (-): Escherichia coli, Salmonella enteritidis, Shigella and two Gram (+): Staphylococcus aureus and Bacillus cereus bacteria. The M. indica bark essential oil was characterized by the association of two major sesquiterpenes: ( E)-β-caryophyllene (60.3%) and α-humulene (36.7%). It presented the lowest ratio of concentration to inhibition zone diameter on all the strains. The aqueous and ethanol extracts of M. indica bark were characterized by high contents of total phenols compounds and high radical scavenging activity compared to the essential oil. Finally, the interesting combination of the antibacterial and antiradical activities of the aqueous M. indica bark extract justifies the traditional use of this plant part in decoction form for the treatment of diarrheal infections.

scholarly journals CHEMICAL COMPOSITION AND HEPATOPROTECTIVE EFFECTS OF POLYPHENOLIC FRACTION FROM RIVEA HYPOCRATERIFORMIS IN PARACETAMOL INDUCED LIVER DAMAGE IN WISTAR ALBINO RATS

2016 ◽  
Vol 8 (10) ◽  
pp. 228
Author(s):  
Shivakumar S. Godipurge ◽  
Naveen J. Biradar ◽  
Jaiprakash S. Biradar ◽  
Nitin Mahurkar
Keyword(s):  
Lipid Peroxidation ◽  
Chemical Composition ◽  
Hydroxyl Radical ◽  
Liver Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Aerial Parts ◽  
Hepatoprotective Effects ◽  
Phenolic And Flavonoid

<p><strong>Objective: </strong>This study was designed to chemical composition and hepatoprotective effects of a polyphenolic fraction of aerial parts of <em>R. hypocrateriformis </em>(PPFRH). It was shown to exhibit strong <em>in vitro</em> lipid peroxidation and scavenging activity against hydroxyl radical.</p><p><strong>Methods: </strong>The chemical composition of a polyphenolic fraction of <em>R. hypocrateriformis</em> was analyzed by High-performance liquid chromatography method. Hepatocellular injuries induced by paracetamol were assessed by liver damage in Wistar albino rat; the hepatoprotective effect was evaluated by biochemical parameters in rat serum, antioxidant hydroxyl radical scavenging activity and lipid peroxidation in liver tissue.</p><p><strong>Results: </strong>The polyphenolic fraction of aerial parts of <em>R. hypocrateriformis</em> for lipid peroxidation is significantly (p&lt;0.05). In the hepatoprotective activity of liver enzymes and hepatic necrosis were significantly (p&lt;0.001) closer to paracetamol. The correlation coefficient between the hydroxyl scavenging radical and total phenolic and flavonoid contents were found to be R² = 0.9045 and R² = 0.8876 suggesting the contribution of phenolic and flavonoid compounds of the polyphenolic fraction of aerial parts of <em>R. hypocrateriformis</em> by 90% and 88% to its radical scavenging activity.</p><p><strong>Conclusion: </strong>The polyphenolic fraction of aerial parts of <em>R. hypocrateriformis</em> possesses a significant protective effect against acute hepatotoxicity induced by paracetamol and which may be due to the phenolic and flavonoid components.</p>

scholarly journals Phytochemical, in-vitro biological and chemo-preventive profiling of Arisaema jacquemontii Blume tuber extracts

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Saira Tabassum ◽  
Muhammad Zia ◽  
Esperanza J. Carcahe de Blanco ◽  
Riffat Batool ◽  
Roohi Aslam ◽  
...  
Keyword(s):  
Cell Lines ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Meoh Extract ◽  
Etoac Extract

Abstract Background Arisaema jacquemontii is traditionally used in treatment of different diseases. In this study, phytochemical, in vitro biological and chemo-preventive screening of A. jacquemontii was carried out to explore its pharmacological potential. Methods The dried tuber of A. jacquemontii was extracted in 11 organic solvent mixture of different polarity. The extracts were screened for phytochemical assays (phenolics and flavonoids), antioxidants potential (free radical scavenging activity, total antioxidant activity, reducing power), biological activities (antibacterial, antifungal, cytotoxic, antileishmanial, protein kinase inhibition), and chemopreventive activities using different cell lines through standard protocols. Results Significant amount phenolic contents were determined in EtOH and MeOH extracts (210.3 ± 3.05 and 193.2 ± 3.15 μg GAE/mg, respectively). Maximum flavonoid content was determined in MeOH extract (22.4 ± 4.04 μg QE/mg). Noteworthy, DPPH scavenging activity was also recorded for MeOH extract (87.66%) followed by MeOH+EtOAc extract (85.11%). Considerable antioxidant capacity (7.8 ± 0.12 μg AAE/mg) and reducing power (3.1 ± 0.15 μg AAE/mg) was observed in extract of MeOH. The LC50 against brine shrimp and leishmanial parasite was found 9.01 and 12.87 μg/mL for n-Hex and CHCl3 extracts, respectively. The highest zone of inhibition against Streptomyces hyphae formation (12.5 ± 1.77 mm) by n-Hex extract. Growth zone of inhibition 13.8 ± 1.08 mm was recorded for EtOAc and MeOH extracts, respectively against Micrococcus luteus while 10.0 ± 0.11 mm for MeOH extract against Aspergillus flavus. In-vitro cytotoxic assay showed that n-Hex extract had higher cytotoxicity against DU-145 prostate cancer and HL-60 cancer cell lines. NF-kB and MTP potential showed 34.01 and 44.87 μg/mL for n-Hex and CHCl3 extracts, respectively in chemo-preventive potential. Conclusion The study concludes that Arisaema jacquemontii bears significant phytochemical activity and pharmacological activities, this plant can be further explored for isolation of active component against a number of aliments.

scholarly journals Correlation between chemical composition and radical scavenging activity of 10 commercial essential oils: Impact of microencapsulation on functional properties of essential oils

2020 ◽  
Vol 13 (8) ◽  
pp. 6815-6827 ◽  
Author(s):  
Hoda Hanem Mohamed Fadel ◽  
Ahmed H. El-Ghorab ◽  
Ahmed M.S. Hussein ◽  
Khaled F. El-Massry ◽  
Shereen Nazeh Lotfy ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Functional Properties ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity

Chemical Composition and TLC-DPPH-Radical Scavenging Activity ofCyperus alternifoliusRottb. Essential Oils

2017 ◽  
Vol 20 (4) ◽  
pp. 1125-1130 ◽  
Author(s):  
Shimaa S. Elshrif ◽  
Abd El-Nasser G. El Gendy ◽  
Abdelsamed I. Elshamy ◽  
Mahmoud I. Nassar ◽  
Hesham R. El-Seedi
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

Wine Chemical Composition and Radical Scavenging Activity of Some Cabernet Franc Clones

2017 ◽  
Vol 18 (4) ◽  
pp. 343-350 ◽  
Author(s):  
Jelena Popovic-Djordjevic ◽  
Boris Pejin ◽  
Aleksandra Dramicanin ◽  
Sonja Jovic ◽  
Dragan Vujovic ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Cabernet Franc

Chemical Composition and Bioactivity of Essential Oil and Extracts of Salvia limbata C.A. Mey (Lamiaceae)

2020 ◽  
Vol 16 (9) ◽  
pp. 1299-1305
Author(s):  
Behnam Mahdavi ◽  
Somaye Ghezi ◽  
Behroz Maleki
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Plant Extracts ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Enterobacter Aerogenes ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Etoac Extract ◽  
Medicinal Uses

Background: This research tried to evaluate the chemical composition of the essential oil of Salvia limbata, and also bioactivity of essential oil and the plant extract. S. limbata is an aromatic plant with medicinal uses, such as analgesic, anti-inflammatory activities. Methods: The essential oils of the flowers of Salvia limbata were obtained using a Clevenger-type apparatus, and its chemical composition was identified by chromatography methods. The study was also conducted to evaluate antioxidant and antibacterial activities of the plant extracts. For this purpose, the leaves and flowers of S. limbata were extracted using methanol and then fractioned by n-hexane and Ethyl Acetate (EtOAc). Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Radical Scavenging Activity (RSA) and the Disc-Diffusion (DD) assay were run to study the bioactivity of the plant extracts. Results: The oil was dominated by the oxygenated hydrocarbon of 5-methylene-2,3,4,4-tetramethylcyclopent- 2-enone (37.1%), oxygenated sesquiterpene of occidol (22.8%), and sesquiterpene hydrocarbon of β-bourbonene (12.1%). The EtOAc extract of the flowers showed the TPC with 66.2±0.62 mg GAE/g (mg of gallic acid equivalents per gram of extract) and RSA with IC50 of 17.96±0.54 μg/mL. Although the EtOAc extract of the leaves exhibited the highest TFC with 61.96±4.73 mg RuE/g (mg of rutin equivalents per gram of extract). The extracts inhibited the growth of Escherichia coli, Citrobacter frurdi, Pseudomonas aeruginosa, and Streptococcus pneumonia. The extracts were inactive against Staphylococcus coagulase, Enterobacter aerogenes, Acinetobacter baumannii, Serratia marcescens, and Klebsiella pneumoni. Conclusion: According to the results, the plant extracts were potent agant in antioxidant activity.

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