A Hypercaloric pellet-diet cycle induces obesity and co-morbidities in wistar rats

The present study was carried to develop and analyze the consequences of hypercaloric pellet-diet cycle that promotes obesity in rats. Male Wistar rats were randomly distributed into two groups that received either normal diet (ND; n =32; 3,5 Kcal/g) or a hypercaloric diet (HD; n =32; 4,6 Kcal/g). The ND group received commercial Labina rat feeding while the HD animals received a cycle of five hypercaloric diets over a 14-week period. The effects of the diets were analyzed in terms of body weight, body composition, hormone-metabolite levels, systolic arterial pressure and glucose tolerance at the 5% significance level. The hypercaloric pellet diet cycle promoted an increase in body weight and fat, systolic arterial pressure and a high serum level of glucose, triacylglycerol, insulin and leptin. The HD group also presented an impaired glucose tolerance. In conclusion, the results of this study show that the hypercaloric pellet-diet cycle promoted obesity in Wistar rats and displayed several characteristics that are commonly associated with human obesity, such as high arterial pressure, insulin resistance, hyperglycaemia, hyperinsulinaemia, hyperleptinaemia and dyslipidaemia.

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Efficacy of Garcinia Cambogia on Body Weight, Inflammation and Glucose Tolerance in High Fat Fed Male Wistar Rats

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2015/12045.5577 ◽

2015 ◽

Cited By ~ 4

Author(s):

Ramalingam Sripradha

Keyword(s):

Body Weight ◽

Glucose Tolerance ◽

Wistar Rats ◽

High Fat ◽

Garcinia Cambogia ◽

Male Wistar Rats

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Utilization of Snakehead Fish Meat for Increasing Kidney Function in Rat Experiencing Physiological Stress

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v10i3.16254 ◽

2018 ◽

Vol 10 (3) ◽

pp. 553-558

Author(s):

Sunarno Sunarno ◽

Hajar Sokmawati ◽

Rully Rahadian

Keyword(s):

Body Weight ◽

Kidney Function ◽

Wistar Rats ◽

Physiological Stress ◽

The Body ◽

Control Group ◽

Kidney Weight ◽

Significance Level ◽

Fish Meat ◽

Male Wistar Rats

Physiological stress for a long time can lead to impaired kidney organ function. Snakehead fish meat are known to contain important nutrients such as albumin, glutamine, glycine, cysteine, and various minerals that can improve kidney function. This study was conducted to examine and analyze the effect of snakehead fish supplementation in feed towards body weight, kidney weight, diameter and number of glomerulus in Wistar rats with physiological stress. This study used 20 male Wistar rats divided into 5 groups consisted of 1 control group (P0) and 4 treatment groups (P1: 5%, P2: 10%, P3: 15%, P4: 20%). The treatments were performed with 4 times repetition. This study used Completely Randomized Design (CRD). The variables measured were body weight, kidney weight, diameter and number of renal glomerulus. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance level. The results of this study were: the supplementation of snakehead fish with 20% of concentration can increase the body weight; concentration 10; 15; and 20% can increase the weight of the kidney; concentrations of 5%, 10%, 15%, and 20% can increase the glomerular diameter in Wistar rats with physiological stress. The supplementation of snakehead fish with concentration of 5% to 20% did not affect the number of glomerulus of Wistar rats with physiological stress. Results of this study is useful as information for people who study the mechanism of kidney repair due to physiological stress.

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Application of Snakehead Fish to Increase Liver Function of Rats after Experiencing Physiological Stress

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v10i1.13957 ◽

2018 ◽

Vol 10 (1) ◽

pp. 109-116

Author(s):

Sunarno Sunarno ◽

Siti Muflichatun Mardiati ◽

Rully Rahadian

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Physiological Stress ◽

The Body ◽

Central Venous ◽

Significance Level ◽

Energy Deficiency ◽

Randomized Design ◽

Male Wistar Rats ◽

Hepatic Histology

Physiological stress due to nutritional deficiency accompanied by excessive activity can cause problems of energy deficiency required by the body. Energy shortage can affect the body weight, as well as the weight and histology of the liver. This study was conducted to examine and analyze the effect of snakehead fish meat supplementation in feed toward body weight, weight and hepatic histology in animals experiencing physiological stress. This study used 20 male Wistar rats consisting of 5 treatments with 4 replications. The treatment in this study consisted of control (P0) and treatment (P1: 5%, P2: 10%, P3: 15%, P4: 20%) groups. This research used Completely Randomized Design (RAL). The variables measured in this study were body weight, hepatic weight, central venous diameter and hepatocytes in the test animals. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance. The result of data analysis showed that fish snakehead fish supplementation in feed gave a significant effect to body weight, weight and histology of animal liver. Concentrations of snakehead fish supplements can increase the body weight, hepatic weight, and hepatic histology which was indicated by higher central venous and hepatocyte diameter values compared to other controls and treatments. The conclusion of this research is the provision of snakehead fish supplement (Channia striata) can increase the body weight, hepatic weight, central venous and hepatocytes diameter of Wistar rats with the most influential concentration of 20%. This evidence is useful in studying the mechanism of liver repair due to stress

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Tissue Deposition of Zinc from a Zinc Chelate and from Inorganic Zinc in Rats

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600027161 ◽

1994 ◽

Vol 1994 ◽

pp. 171-171 ◽

Cited By ~ 1

Author(s):

Ronan Power ◽

Kevin Cashman ◽

Albert Flynn

Keyword(s):

Amino Acids ◽

Tissue Distribution ◽

Wistar Rats ◽

Gel Filtration ◽

Normal Diet ◽

Farm Animals ◽

Trace Minerals ◽

Inorganic Salts ◽

Male Wistar Rats ◽

Differential Tissue

Some reports have suggested differential tissue deposition of dietary trace minerals such as Zinc (Zn) when supplied to farm animals either chelated to amino acids or as inorganic salts. To test this hypothesis, an experiment was conducted to determine the ultimate tissue distribution of Zinc in rats fed either a radioactively-labeled 65Zn-chelate or 65ZnSO4. The 65Zn-chelate was prepared by heating a solution of 65ZnSO4 and an equimolar mixture of glycine and methionine for 5 minutes at 90°C. The resulting chelate was then separated from unincorporated 65ZnSO4 by gel filtration chromatography. Ten 25-d old male wistar rats (mean weight 34.5 g) were randomized by weight into two groups (n = 5/group), fasted for 18 hours and given 0.4 ml (8 μg Zn, 1 μCi65Zn) of one or other labelled solution by gavage. Four hours later, animals were returned to their normal diet for the duration of the experiment. The 65Zn activity of the animals was determined two hours after administration and daily thereafter for 7 days.

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Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

African Journal of Food, Agriculture, Nutrition and Development ◽

10.18697/ajfand.95.19950 ◽

2020 ◽

Vol 20 (07) ◽

pp. 16984-16996

Author(s):

MMC Anyakudo ◽

◽

DO Adeniji ◽

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Treated Group ◽

Morphological Features ◽

High Protein ◽

Control Group ◽

Formulated Diet ◽

Low Carbohydrate ◽

Male Wistar Rats ◽

And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

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Abstract 404: NLR Family, Pyrin Domain-containing 3 Knockout Rescues Cardiac Dysfunction Induced by High-fat Diet Feeding

Circulation Research ◽

10.1161/res.121.suppl_1.404 ◽

2017 ◽

Vol 121 (suppl_1) ◽

Author(s):

Matthew R Peterson ◽

Samantha Haller ◽

Tracy Ta ◽

Luiza Bosch ◽

Aspen Smith ◽

...

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Inflammatory Response ◽

Glucose Tolerance ◽

High Fat Diet ◽

Fasting Blood Glucose ◽

Left Ventricular ◽

Normal Diet ◽

High Fat ◽

Pyrin Domain

NLR family, pyrin domain-containing 3 (NLRP3) is a pattern recognition receptor responsible for perpetuating an inflammatory response through production of pro-inflammatory cytokines IL-1β and IL-18. It has been implicated in the sustained inflammatory response in obesity and multiple cardiovascular disease conditions. In order to investigate NLRP3 as a potential therapeutic target in metabolic syndrome, C57BL/6 wild-type (WT) and NLRP3 knockout (NLRP3-\-) mice were fed a normal diet (ND; 12% fat chow) or a high fat diet (HFD; 45% fat chow) for 5 months. At 5 months, echocardiography and glucose tolerance tests (GTTs) were performed. Cardiac function assessed by fractional shortening (FS) was significantly impaired by HFD feeding in the WT group (0.335 HFD vs. 0.456 ND; p<0.05) but not in the NLRP3-\- (0.449 HFD vs. 0.492 ND; p>0.05). FS was higher in NLRP3-\-HFD than in WT-HFD (p<0.05). Two-dimensional analysis shows the FS difference between NLRP3-\-HFD and WT-HFD was primarily explained by the difference in left ventricular end-systolic dimension (0.2716 cm WT vs. 0.1883 cm NLRP3-\-; p<0.05). Glucose tolerance measured by area under the curve (AUC) was significantly impaired by HFD feeding for both WT (23183 ND vs. 57298 HFD; p<0.001) and NLRP3-\- (23197 ND vs. 44626 HFD; p<0.001), but significantly better in the NLRP3-\-HFD than in WT-HFD (p<0.01). HFD feeding increased fasting blood glucose (FBG) for both WT (97.7 mg . dl -1 ND vs. 164.7 mg . dl -1 HFD; p<0.01) and NLRP3-\- (80.50 mg . dl -1 ND vs. 108.8 mg . dl -1 HFD; p<0.05), but significantly less in NLRP3-\- mice (NLRP3-\- vs. WT; p<0.05). For GTTs, body weight was significantly higher in the WT than NLRP3-\- fed HFD (47.93 g vs. 36.5 g; p<0.001). Body weight explained 92% of variation in glucose tolerance (p<0.0001) and 69% of variation in fasting blood glucose (p<0.0001). WT-HFD averaged 1.31X heavier than NLRP3-\-HFD, while the AUC for the IGTT was 1.28X larger for the WT-HFD than NLRP3-\-HFD. Body weights were not significantly different between genotypes at the time of echo. The results suggest that knockout of NLRP3 may be protective against HFD induced cardiovascular dysfunction. A protective effect on glucose tolerance is not strongly supported.

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Hypothalamic paraventricular nucleus lesions do not prevent anorectic effect of exercise in male rats

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1990.259.3.r579 ◽

1990 ◽

Vol 259 (3) ◽

pp. R579-R584 ◽

Cited By ~ 4

Author(s):

S. Rivest ◽

D. Richard

Keyword(s):

Body Weight ◽

Energy Balance ◽

Paraventricular Nucleus ◽

Wistar Rats ◽

Energy Gain ◽

Hypothalamic Paraventricular Nucleus ◽

Male Rats ◽

Hypothalamic Nucleus ◽

Serum Corticosterone ◽

Male Wistar Rats

The effects of a hypothalamic paraventricular nucleus (PVN) lesion on energy balance were investigated in exercise-trained rats. Male Wistar rats weighing initially 250 g were divided into four groups. Two groups of rats underwent a bilateral PVN lesion, whereas the two remaining groups were sham operated. The PVN lesions were done electrolytically. One group from each surgical treatment was exercised, while the other group was kept in sedentary conditions. Rats were exercised on a rodent motor-driven treadmill at moderate intensity, 1 h/day for 21 consecutive days. Food intake and body weight were measured each day during the study. At the end of the treatment period, rats were killed, and carcasses were analyzed for their energy content. Serum corticosterone was measured by a competitive protein-binding assay. Energy gain and energy intake were lower in exercised rats than in sedentary controls, regardless of whether they were sham or PVN lesioned. Concurrently, there was no difference in the energy gain between PVN-lesioned and sham-operated rats, despite the fact that PVN-lesioned rats ended the experiment with a larger body weight than the sham-lesioned animals. Serum corticosterone levels were lower in PVN-lesioned rats than in sham-lesioned rats. In conclusion, the present results indicate that the PVN, the hypothalamic nucleus predominantly controlling the pituitary-adrenal axis activity, is not a prominent structure in the regulation of energy balance in exercised male Wistar rats.

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Model of spontaneous obesity in aging male Wistar rats

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1990.259.6.r1117 ◽

1990 ◽

Vol 259 (6) ◽

pp. R1117-R1125 ◽

Cited By ~ 10

Author(s):

F. D. Newby ◽

M. DiGirolamo ◽

G. A. Cotsonis ◽

M. H. Kutner

Keyword(s):

Body Weight ◽

Lipid Content ◽

Wistar Rats ◽

Lipid A ◽

Curvilinear Relationship ◽

Body Lipid ◽

Fat Depots ◽

Male Wistar Rats ◽

Total Body ◽

Relationship Of

We analyzed retrospectively data from 148 chow-fed male Wistar rats killed between the age of 6 wk and 2 yr while varying in body weight from 136 to 917 g. The purpose of this study was to clarify the relationship of body weight and body lipid content with the composition and cellularity of the epididymal and retroperitoneal fat depots. A positive linear association was found between body weight and body water or fat-free dry residue, whereas total body lipid exhibited a curvilinear relationship with body weight. The weight of the epididymal pads was linearly related to body weight but not to body lipid. In contrast, retroperitoneal pad weight was exponentially related to body weight and paralleled total body lipid. A strong linear correlation was found between total body lipid and weight (r = 0.959) or depot lipid content (r = 0.967) of the retroperitoneal fat pads. In this rat model of aging and spontaneous obesity, significant regional differences exist in adipose depot composition and cellularity. A practical outcome of this study is a simple and accurate prediction of body lipid content from the gravimetric determination of the retroperitoneal fat depots.

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Vitamin C decreases the obesogenic and hyperglycemic effect of invert sugar in prediabetic rats

Revista de Nutrição ◽

10.1590/1678-98652017000100003 ◽

2017 ◽

Vol 30 (1) ◽

pp. 23-32 ◽

Cited By ~ 1

Author(s):

Patrícia MOLZ ◽

Alana Nunes RAEL ◽

Maiara de Queiroz FISCHER ◽

Luana Beatriz LIMBERGER ◽

Daniel PRÁ ◽

...

Keyword(s):

Vitamin C ◽

Glucose Tolerance ◽

Body Mass ◽

Wistar Rats ◽

Area Under The Curve ◽

Tolerance Test ◽

Invert Sugar ◽

Future Studies ◽

Fasting Glycemia ◽

Male Wistar Rats

ABSTRACT Objective: To evaluate whether vitamin C can help to prevent obesity and hyperglycemia in Wistar rats treated with excess invert sugar to induce prediabetes. Methods: One hundred-day-old Male Wistar rats with a mean weight of 336.58±23.43g were randomly assigned to the following groups: (1) control, receiving water (C); (2) invert sugar control, receiving a 32% watery solution of invert sugar; (3) vitamin C control, receiving a watery solution of vitamin C (60mg/L), and (4) vitamin C plus invert sugar, receiving a watery solution of vitamin C and invert sugar. All animals had access to chow and water ad libitum and were treated for 17 weeks. Prediabetes was assessed according to two criteria: obesity (based on body mass indexand peritoneal fat content) and impaired glucose tolerance (assessed by the intraperitoneal glucose tolerance test and expressed as area under the curve) . Results: Group invert sugar control gained significantly more weight (p=0.035) and visceral fat (p<0.001) than groups vitamin C control and vitamin C plus invert sugar. Consequently, groups vitamin C control and vitamin C plus invert sugar had gained as little body mass index as group C by the end of the experiment. Vitamin C decreased the fasting glycemia of both groups supplemented with vitamin C and normalized the glucose tolerance of group vitamin C plus invert sugar, whose area under the curve matched that of group C. Conclusion: Vitamin C has anti-obesogenic and glycemia-lowering effects in Wistar rats, which might be promising to prediabetics. Future studies are needed to understand the anti-obesogenic and anti-hyperglycemic mechanisms of vitamin C in prediabetes.

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