scholarly journals Virulence factors of uropathogenic Escherichia coli from a University Hospital in Ribeirão Preto, São Paulo, Brazil

2006 ◽  
Vol 48 (4) ◽  
pp. 185-188 ◽  
Author(s):  
Edilene Santo ◽  
Claudia Macedo ◽  
José Moacir Marin
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Tertiary Care ◽  
Uropathogenic Escherichia Coli ◽  
University Hospital ◽  
Adhesive Systems ◽  
Specific Primers ◽  
Polymerase Chain ◽  
The Difference

The aim of the study was to determine the occurrence of virulence genes expressing fimbriae, production of hemolysin, colicin and aerobactin among a hundred Escherichia coli isolates obtained from in-and outpatients of a tertiary-care teaching hospital, between July and August 2000, showing clinical and laboratory signs of urinary tract infection (UTI). The presence of genes (pap, afa, sfa) for fimbriae expression was assayed using specific primers in a polymerase chain reaction. Among the isolates studied, the prevalence of the virulence factors was 96.0%, 76.0%, 24.0%, for hemolysin, aerobactin and colicin, respectively; the prevalence of genes coding for fimbrial adhesive systems was 32.0%, 19.0% and 11.0% for pap, sfa and afa respectively. The strains isolated from the outpatients displayed a greater number of virulence factors compared to those from hospitalized subjects, emphasizing the difference between these two kinds of patients.

scholarly journals Prevalence of virulence factors in Escherichia coli strains isolated from the genital tract of healthy cows

2007 ◽  
Vol 59 (3) ◽  
pp. 564-568 ◽  
Author(s):  
D. Resende ◽  
E. Santo ◽  
C. Macedo ◽  
J.M. Marin
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Genital Tract ◽  
Virulence Genes ◽  
Clinical Signs ◽  
Chain Reaction ◽  
Specific Primers ◽  
E Coli ◽  
Bacterial Pathogenicity ◽  
Polymerase Chain

The prevalence of virulence genes expressing fimbriae, production of hemolysin, colicin and aerobactin, was determined in Escherichia coli isolates from healthy cow’s genital tract not showing clinical signs of infection. The presence of fimbriae expression genes (pap, sfa, afa) was assayed using specific primers in a polymerase chain reaction; none were detected in any of the isolates. Yet, a prevalence of 90.4%, 69.8%, and 28.5% of virulence factors for colicin, hemolysin and aerobactin respectively, was detected in the isolates. Analysis of the bacterial pathogenicity of isolates from the bovine genital tract may contribute towards the understanding of E. coli behavior.

Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

2019 ◽  
Vol 19 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Hassan Valadbeigi ◽  
Elham Esmaeeli ◽  
Sobhan Ghafourian ◽  
Abbas Maleki ◽  
Nourkhoda Sadeghifard
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multiplex Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Uropathogenic Escherichia Coli ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

scholarly journals Molecular characterization of virulence factors in Aeromonas hydrophila obtained from fish

2012 ◽  
Vol 32 (8) ◽  
pp. 701-706 ◽  
Author(s):  
Samira T.L. Oliveira ◽  
Gisele Veneroni-Gouveia ◽  
Mateus M. Costa
Keyword(s):  
Virulence Factors ◽  
Aeromonas Hydrophila ◽  
Nile Tilapia ◽  
Virulence Genes ◽  
Saline Solution ◽  
Specific Primers ◽  
In Vivo Tests ◽  
Polymerase Chain

Multiple factors can be involved in the virulence processes of Aeromonas hydrophila. The objective of the present paper was to verify the presence of aerolysin, hidrolipase, elastase and lipase virulence genes through the polymerase chain reaction (PCR) in A. hydrophila isolates obtained from fish of the São Francisco River Valley, and to evaluate virulence according to the presence of these genes in Nile tilapia fingerlings. One hundred and fourteen isolates from the bacteria were used. DNA was heat extracted and PCR undertaken using specific primers described in the literature. For in vivo tests Nile tilapia fingerlings were used. From the PCR tests, negative isolates for all genes tested were selected, positive isolates for two genes (aerolysin and elastase) and positive for the four genes tested. These were inoculated at a concentration of 10(8) UFC/ml into the tilapias, considered as treatments; another group of animals was used as control (with inoculation of saline solution). In all, 12 distinct standards regarding the presence of virulence factors in isolates from A. hydrophila, were observed. Of the 114 isolates analyzed, 100 (87.72%) presented at least one of the virulence factors under study. The virulence factors were widely distributed among the A. hydrophila isolates. Aerolysin was the most frequent virulence factor present in the isolates analyzed. A. hydrophila led to the mortality of the Nile tilapia fingerlings, regardless of the absence or quantity of virulence genes tested.

scholarly journals Survival of enteroaggregative Escherichia coli and Vibrio cholerae in frozen and chilled foods

2015 ◽  
Vol 9 (08) ◽  
pp. 837-843 ◽  
Author(s):  
Diana Elizabeth Waturangi ◽  
Steven Amadeus ◽  
Yustinus Ericko Kelvianto
Keyword(s):  
Escherichia Coli ◽  
Vibrio Cholerae ◽  
Room Temperature ◽  
Virulence Genes ◽  
Food Samples ◽  
Food Preservation ◽  
Poor Survival ◽  
Specific Primers ◽  
Polymerase Chain ◽  
Almost All

Introduction: Enteroaggregative Escherichia coli (EAEC) and Vibrio cholerae are common bacteria that infect people in developing countries. Nowadays, food preservation by freezing and refrigeration are very common practices that extend the shelf life of food products. Unfortunately, EAEC and V. cholerae are suspected to survive at low temperatures. Methodology: Various foods and beverages used as samples were artificially inoculated with EAEC and V. cholerae pure colonies and incubated at room temperature (27°C), refrigeration temperature (4°C), and frozen temperature (-20°C) for one week, two weeks, one month, two months, and three months. The survival of these bacteria was confirmed by polymerase chain reaction (PCR) with specific primers to detect their virulence genes (aggR for EAEC and toxR for V. cholerae) that represent the presence of these bacteria. Results: This study showed that EAEC was able to survive in all food samples used in this study for at least three months under room temperature, refrigeration temperature, and frozen temperature. V. cholerae had poor survival in almost all food samples over two months under room temperature and over three months under refrigeration temperature. V. cholerae was able to survive in all food samples for at least three months under frozen temperature. Conclusions: The survival of EAEC and V. cholerae in this study should increase our awareness of how hazardous these bacteria are. Therefore, in order to improve food safety and hygiene, advanced preservation and sterilization methods should be discovered to prevent contamination with and survival of EAEC and V. cholerae.

scholarly journals Detection of Urovirulence Genes (eae,E-hly,α-hly) of Uropathogenic Escherichia coli by Specific PCR

2010 ◽  
Vol 4 (1) ◽  
pp. 44-54
Author(s):  
Rawa’a Al-Chalabi ◽  
Ayad Al –Ubaidy ◽  
Muneera Al- Ibadi
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Health Problem ◽  
Intestinal Flora ◽  
Uropathogenic Escherichia Coli ◽  
Specific Primers ◽  
Specific Pcr ◽  
Erythrocyte Lysis ◽  
The World ◽  
Different Types

Urinary tracts infection remains a common troublesome health problem in the world. Although E.coli is normal intestinal flora but considered as the main important opportunistic active uropathogene because of it’s pathogenesity which referred to it’s different virulence factors like hemolysin, biofilm, enterotoxins, shiga like toxins, cytotoxic necrotizing factor and others. Seventy seven E.coli isolates which isolated from 125 midstream urine samples of patients suffering from different types of UTIs. The results showed that 44 isolates 57% were produced hemolysin. These isolates were differed in the efficiency of erythrocyte lysis because which depend on type of hemolysin and source of blood. Seventy two isolates 90.9% were formed biofilm with variety in thickness of biofilm layer. Each isolate produce hemolysin also formed biofilm. Presence of genes encoded for virulence traits of UPEC (hemolysin, biofilm) were examined by PCR with specific primers. The results showed that the percentage of α-hly gene encoded for α-hemolysin 50% while percentage of eae gene encoded for intimin and E-hly gene encoded for enterohemolysin were 40% and 20% respectively.

scholarly journals Antimicrobials resistance patterns and the presence of stx1, stx2 and eae in Escherichia coli

2015 ◽  
Vol 16 (2) ◽  
pp. 308-316 ◽  
Author(s):  
Gustavo Lacerda Homem ASSUMPÇÃO ◽  
Marita Vendovelli CARDOZO ◽  
Lívia Gerbase BERALDO ◽  
Renato Pariz MALUTA ◽  
Joviany Talita SILVA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Chain Reaction ◽  
Resistance Patterns ◽  
Antimicrobial Susceptibility Tests ◽  
Polymerase Chain ◽  
Susceptibility Tests

The objectives of this study were to investigate whether antimicrobial resistance (AMR) or the presence of resistance genes was associated with the occurrence of the virulence genes, stx1, stx2 andeae. Three virulence genes and 11 AMR phenotypes were examined using polymerase chain reaction (PCR) and antimicrobial susceptibility tests. From 800 samples collected in this study, 561 samples were isolatesE. coli strains , being: 90 (16.0%) carriers ofstx1, 97 (17.3%) of stx2 and 45 (8.0%) ofeae genes singly. Thirty seven (6.6%) isolates were carriers of stx1 and stx2, 110 (19.6%) were carriers of stx1 and eae and 67 (11.9%) were carriers of stx2 and eae. The most common virulence gene detected was stx1followed bystx2. The findings showed no relationship between presence of virulence factors and antimicrobial resistance. Also was not found relationship between serogroup and virulence factors.

scholarly journals Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer

2020 ◽  
Vol 50 (2) ◽  
Author(s):  
Juliana Maria Avanci Agostinho ◽  
Marita Vedovelli Cardozo ◽  
Mariana Monezi Borzi ◽  
José Moacir Marin
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Organic Waste ◽  
Virulence Genes ◽  
Organic Fertilizer ◽  
Chain Reaction ◽  
E Coli ◽  
Chicken Litter ◽  
Polymerase Chain

ABSTRACT: Brazilian poultry industry generates large amounts of organic waste, such as chicken litter, which is often used in agriculture. Among the bacteria present in organic fertilizer are members of the Enterobacteriaceae family, such as Escherichia coli. The aim of this study was to analyze the profile of virulence factors and antimicrobial resistance of E. coli isolates from avian organic fertilizer. A total of 47 E. coli strains were tested through Polymerase chain reaction to detect virulence genes (hlyF, iss, ompT, iutA and iroN). Fourteen antimicrobials were used to test antimicrobial susceptibility in the strains. Genes characteristic of Avian Pathogenic E. coli (APEC) were reported among the strains, with the hlyF, iss and ompT genes being the most prevalent. The isolates showed high resistance (˃50%) to tetracycline, gentamicin, cefotaxime, nitrofurantoin, trimethoprim-sulfamethoxazole and ampicillin. Multidrug resistance was reported in a great number of strains (>70%). The results showed the presence of APEC cells with virulence genes and antimicrobial resistance after 15 days of the windrowing process in poultry houses, it means this process should be improved to eliminate these cells.

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