scholarly journals Transformation of Xanthomonas axonopodis pv. citri by electroporation

2005 ◽  
Vol 30 (3) ◽  
pp. 292-294 ◽  
Author(s):  
Alexandre M. do Amaral ◽  
Cristiane P. Toledo ◽  
Juliana C. Baptista ◽  
Marcos A. Machado
Keyword(s):  
Electrical Resistance ◽  
High Efficiency ◽  
Electrical Parameters ◽  
Electrical Field ◽  
Electrical Field Strength ◽  
Xanthomonas Axonopodis ◽  
Additional Information ◽  
Complete Sequencing ◽  
Selection Of ◽  
Selection Of Transformants

This study describes the use of electroporation for transforming Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus (Citrus spp.) canker. It also evaluates the methodology used for this species under different electrical parameters. The bacterium used in the study (Xac 306) was the same strain used for recent complete sequencing of the organism. The use of a plasmid (pUFR047, gentamycin r) is reported here to be able to replicate in cells of Xac. Following the preparation and resuspension of competent cells of Xac at a density of ~4 x 10(10) cfu/ml, in 10% glycerol, and the addition of the replicative plasmid, an electrical pulse was applied to each treatment. Selection of transformants showed a high efficiency of transformation (1.1 x 10(6) transformants/mug DNA), which indicates an effective, and inverse, combination between electrical resistance (50 W) and capacitance (50 µF) for this species, with an electrical field strength of 12.5 kV.cm-1 and 2.7-ms pulse duration. Besides the description of a method for electroporation of Xac 306, this study provides additional information for the use of the technique on studies for production of mutants of this species.

Development of an AC Electrical Field Tribochemical Polishing Technique to Promote High-Efficiency Polishing for Glass Substrates

2012 ◽  
Vol 78 (4) ◽  
pp. 316-320
Author(s):  
Hiroshi IKEDA ◽  
Yoichi AKAGAMI ◽  
Michio UNEDA ◽  
Osamu OHNISHI ◽  
Syuhei KUROKAWA ◽  
...  
Keyword(s):  
High Efficiency ◽  
Electrical Field ◽  
Glass Substrates

scholarly journals Design Concepts for NiTiNOL Wires to Function as a Sensor

2021 ◽  
Author(s):  
D. Josephine Selvarani Ruth
Keyword(s):  
Shape Memory Alloy ◽  
Electrical Resistance ◽  
Nickel Titanium ◽  
The Other ◽  
Sensing Element ◽  
Proper Selection ◽  
Design Concepts ◽  
Mechanical Element ◽  
Selection Of ◽  
Inverse Hysteresis

AbstractNickel Titanium Naval Ordinance Laboratory (NiTiNOL) is widely called as a shape memory alloy (SMA), a class of nonlinear smart material inherited with the functionally programmed property of varying electrical resistance during the transformation enabling to be positioned as a sensing element. The major challenge to instrument the SMA wires is to suppress the wires’ nonlinearity by proper selection of two important factors. The first factor is influenced by the mechanical biasing element and the other is to identify the sensing current for the sensing device (SMA wires + biasing). This paper focuses on developing SMA wires for sensing in different orientation types and configurations by removing the non-linearity in the system’s output by introducing inverse hysteresis to the wires through the passive mechanical element.

scholarly journals The Relationship between Soil Electrical Parameters and Compaction of Sandy Clay Loam Soil

Agriculture ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Katarzyna Pentoś ◽  
Krzysztof Pieczarka ◽  
Kamil Serwata
Keyword(s):  
Electrical Conductivity ◽  
Magnetic Susceptibility ◽  
Precision Agriculture ◽  
Soil Compaction ◽  
Soil Layer ◽  
Soil Parameters ◽  
Soil Electrical Conductivity ◽  
Electrical Parameters ◽  
Management Zones ◽  
Additional Information

Soil spatial variability mapping allows the delimitation of the number of soil samples investigated to describe agricultural areas; it is crucial in precision agriculture. Electrical soil parameters are promising factors for the delimitation of management zones. One of the soil parameters that affects yield is soil compaction. The objective of this work was to indicate electrical parameters useful for the delimitation of management zones connected with soil compaction. For this purpose, the measurement of apparent soil electrical conductivity and magnetic susceptibility was conducted at two depths: 0.5 and 1 m. Soil compaction was measured for a soil layer at 0–0.5 m. Relationships between electrical soil parameters and soil compaction were modelled with the use of two types of neural networks—multilayer perceptron (MLP) and radial basis function (RBF). Better prediction quality was observed for RBF models. It can be stated that in the mathematical model, the apparent soil electrical conductivity affects soil compaction significantly more than magnetic susceptibility. However, magnetic susceptibility gives additional information about soil properties, and therefore, both electrical parameters should be used simultaneously for the delimitation of management zones.

New Development in High Efficiency Deep Grinding

2012 ◽  
Author(s):  
Andre D. L. Batako ◽  
Valery V. Kuzin ◽  
Brian Rowe
Keyword(s):  
Machine Tool ◽  
High Efficiency ◽  
Machining Process ◽  
Depth Of Cut ◽  
Removal Rates ◽  
Workpiece Surface ◽  
New Development ◽  
Cutting Processes ◽  
Selection Of ◽  
Made In

High Efficiency Deep Grinding (HEDG) has been known to secure high removal rates in grinding processes at high wheel speed, relatively large depth of cut and moderately high work speed. High removal rates in HEDG are associated with very efficient grinding and secure very low specific energy comparable to conventional cutting processes. Though there exist HEDG-enabled machine tools, the wide spread of HEDG has been very limited due to the requirement for the machine tool and process design to ensure workpiece surface integrity. HEDG is an aggressive machining process that requires an adequate selection of grinding parameters in order to be successful within a given machine tool and workpiece configuration. This paper presents progress made in the development of a specialised HEDG machine. Results of HEDG processes obtained from the designed machine tool are presented to illustrate achievable high specific removal rates. Specific grinding energies are shown alongside with measured contact arc temperatures. An enhanced single-pole thermocouple technique was used to measure the actual contact temperatures in deep cutting. The performance of conventional wheels is depicted together with the performance of a CBN wheel obtained from actual industrial tests.

Research and Application of High-Efficiency Multi-Cyclone Dust Removal

2011 ◽  
Vol 109 ◽  
pp. 400-404
Author(s):  
Yan Hong Yang ◽  
Da Fu Ni
Keyword(s):  
Structural Design ◽  
High Efficiency ◽  
Dust Removal ◽  
Working Principle ◽  
Selection Of

Performance and working principle of high-efficiency multi-cyclone were analyzed, and the structural design shortage of original high-efficiency multi-cyclone was pointed out. Its structure was researched and designed, including determination of setting chamber and pipe number, selection of material and the design of cyclones.

From Coarse to Fine: An Augmented Reality Based Dynamic Inspection Method for Visualized Railway Routing of Freight Cars

2021 ◽  
Author(s):  
Bing Yi ◽  
Renkai Sun ◽  
Long Liu ◽  
Yongfeng Song ◽  
Yinggui Zhang
Keyword(s):  
Augmented Reality ◽  
High Efficiency ◽  
Low Cost ◽  
Digital Data ◽  
Route Selection ◽  
Inspection Method ◽  
Envelope Model ◽  
Freight Car ◽  
Cargo Transportation ◽  
Selection Of

Abstract It is a challenge for the dynamic inspection of railway route for freight car transporting cargo that out-of-gauge. One possible way is using the inspection frame installed in the inspection train to simulate the whole procedure for cargo transportation, which costs a lot of manpower and material resources as well as time. To overcome the above problem, this paper proposes an augmented reality (AR) based dynamic inspection method for visualized railway routing of freight car with out-of-gauge. First, the envelope model of the dynamic moving train with out-of-gauge cargo is generated by using the orbital spectrum of the railway, and the envelope model is matched with a piece of homemade calibration equipment located on the position of the railway that needs to be inspected. Then, the structure from motion (SFM) algorithm is used to reconstruct the environment where the virtual envelope model occludes the buildings or equipment along the railway. Finally, the distance function is adopted to calculate the distance between the obstacle and the envelope of the freight car with out-of-gauge, determining whether the freight car can pass a certain line. The experimental results show that the proposed method performs well for the route selection of out-of-gauge cargo transportation with low cost, high precision, and high efficiency. Moreover, the digital data of the environments along the railway and the envelope of the freight car can be reused, which will increase the digitalization and intelligence for route selection of out-of-gauge cargo transportation.

Assessment of efficiency of distribution centre at different variants of freight traffic consolidation

2021 ◽  
pp. 22-27
Author(s):  
Elena Dmitrievna Pserovskaya ◽  
◽  
Anna Pavlovna Grefenshteyn ◽  
Keyword(s):  
Potential Benefit ◽  
High Efficiency ◽  
Positive Influence ◽  
Correct Selection ◽  
Freight Traffic ◽  
Distribution Centre ◽  
Selection Of

For the determination of a potential benefit from creation of a distribution centre the paper proposes a method and presents results of calculation of a decrease of total number of rides and total run of automobiles at various variants of goods consolidation. The authors have established a positive influence of the centre on intracity transportation. At the same time, high efficiency of the considered technology can be reached by involvement of a bigger number of consignees and by consolidation of a relatively small number of goods batches in one automobile at efficient routing and correct selection of location of a distribution centre.

scholarly journals Use of factors of photoperiod duration and growth regulators to increase the yield of mixoploid plants in C0 generation in meadow clover (Trifolium pratense L.)

2021 ◽  
Vol 901 (1) ◽  
pp. 012024
Author(s):  
M Yu Novoselov ◽  
L V Drobysheva ◽  
O A Starshinova
Keyword(s):  
Apical Dominance ◽  
High Efficiency ◽  
Trifolium Pratense ◽  
Morphological Changes ◽  
Continuous Illumination ◽  
Experimental Plant ◽  
Trifolium Pratense L ◽  
Naphthylacetic Acid ◽  
Spraying Plants ◽  
Selection Of

Abstract It has been established that the studied mode of cultivation of meadow clover under controlled environmental conditions, with continuous illumination with an intensity of 18-20 thousand lux and a constant temperature of + 25 °C, causes significant morphological changes in meadow clover. Compared with the optimal (control), the proposed growing regime halves the development time of plants and by the same amount reduces the severity of morphological traits, while simultaneously causing apical dominance in 54% of plants. Spraying plants with phytohormone in the form of naphthylacetic acid in all studied concentrations reduced the growing season by 4-8 days and increased pollen fertility by 8-10%. The optimal concentration of naphthylacetic acid (NLA) was revealed to suppress the growth of secondary meristems in meadow clover under the conditions of an experimental plant growing regime. The most significant effect on the manifestation of apical dominance was shown by the use of naphthylacetic acid at a concentration of 0.001%. The combined use of the proposed growing regime and treatment with naphthylacetic acid at a concentration of 0.001% increased the yield of plants with apical dominance from 54.5% to 76%. The developed method has shown high efficiency for the creation of tetraploid forms of meadow clover. When growing clover C0 generation and selection of mixoploid plants, the yield of mixoploids increased 5.4 times, the survival rate of seedlings increased by 21%, the time to obtain one generation and the selection of mixoploids decreased by 58%.

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