In Vitro evaluation of microleakage of different materials used as pit-and-fissure sealants

The aim of this study was to evaluate in vitro the marginal microleakage of different materials used as pit-and-fissure sealants (Delton, Filtek Flow, Dyract Flow and Vitremer). Fifty-six extracted sound human third molars were randomly assigned to 4 groups (n=14). After sealant placement, the teeth were thermocycled (500 cycles; 5ºC, 37ºC and 55ºC), isolated, immersed in 2% buffered methylene blue dye for 4 h, included in acrylic resin and sectioned longitudinally in a buccolingual direction. The sections were analyzed for leakage using an stereomicroscope. A 4-criteria ranked scale was used to score dye penetration. All materials exhibited dye penetration to some extension and no statistically significant difference was observed among the groups (p>0.05). In conclusion, the findings of this study showed that a flowable composite resin, a flowable compomer and resin-modified glass ionomer placed on occlusal pits and fissures had similar marginal sealing as the unfilled self-cured resin-based sealant.

Download Full-text

Ameloplasty is counterproductive in reducing microleakage around Resin Modified Glass Ionomer and Resin based fissure sealants

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.36.3.1268 ◽

2020 ◽

Vol 36 (3) ◽

Author(s):

Tabinda Nawaz Khan ◽

Farhan Raza Khan ◽

Syed Yawar Ali Abidi

Keyword(s):

Glass Ionomer Cement ◽

Ordinal Scale ◽

P Value ◽

Glass Ionomer ◽

Fissure Sealants ◽

Human Teeth ◽

Fissure Sealant ◽

Significant Difference ◽

Resin Modified Glass Ionomer

Objective: To compare the microleakage around resin modified glass ionomer cement (RMGIC) based sealants and flowable resin based sealants placed with or without ameloplasty in extracted human teeth. Methods: This in-vitro experimental study was conducted at the Operative Dentistry Department, Dow University of Health Sciences, Karachi, Pakistan from June 2017 to December 2018. Sixty extracted human molars and premolars were assigned to four groups (n=15) each, according to the type of fissure sealant (flowable resin based sealant or resin modified glass ionomer based sealant) used and either placed with or without ameloplasty. Specimens were thermocycled and then immersed in 1% methylene blue for 24 hours. Specimens were then sectioned and examined using stereo-microscope (50X) for microleakage that was scored on an ordinal scale. Mann-Whitney U test and Ordinal regression were applied. Level of significance kept at 0.05. Results: There was a statistically significant difference (p-value <0.001) between the two sealant types for the microleakage scores. Sealants placed with ameloplasty demonstrated significantly higher microleakage values (p-value <0.001). Conclusion: Microleakage was found to be more pronounced in RMGIC based sealants compared to the resin based sealants. Ameloplasty resulted in higher leakage around the sealants irrespective of the chemistry of material. doi: https://doi.org/10.12669/pjms.36.3.1268 How to cite this:Khan TN, Khan FR, Abidi SYA. Ameloplasty is counterproductive in reducing microleakage around Resin Modified Glass Ionomer and Resin based fissure sealants. Pak J Med Sci. 2020;36(3):---------. doi: https://doi.org/10.12669/pjms.36.3.1268 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download Full-text

FISSURE SEALANT INTERFACE MICROLEAKAGE USING ONE STEP ADHESIVES

ODONTO Dental Journal ◽

10.30659/odj.1.1.49-52 ◽

2014 ◽

Vol 1 (1) ◽

pp. 49

Author(s):

Sandy Christiono

Keyword(s):

Blue Dye ◽

Fissure Sealant ◽

Whitney Test ◽

Dye Penetration ◽

Mann Whitney Test ◽

Group I ◽

Significant Difference ◽

One Step ◽

Group Ii

Background:This study evaluated the efficacy of a one step adhesives in reducing microleakage after water storage and thermocycling. Method: eighteen freshly extracted caries-free human premolars were used. The teeth were randomly divided into two groups; Group I: control etch adhesive (GC),Group II: G Bond One step adhesive (GC) The teeth were restored using helioseal Vivadent. Group II Each layer was cured using the Spectrum 800 curing light (Dentsply/Caulk) for 20 seconds at 600mW/cm2. The teeth were stored in artifisial saliva for 7 days in incubator. Samples were thermocycled 250x between 5°C and 55°C with a dwell of 30 seconds, then placed in a 0.5% methylene blue dye solution for 24 hours at 37°C. Samples were sectioned longitudinally and evaluated for microleakage at the occlusal and gingival margins under a microscope at 40x magnification. Dye penetration was scored: 0: no microleakage visible, score 1: microleakage up to half of the fissure, score 2: microleakage more than half of the fissure. Result: Mann-Whitney test was used to demonstrate significantly more dye penetration in Group I and Group II. When comparing the scores for two group, the Mann Whitney test showed no significant difference in dye penetration for etch and One step adhesive (p>0.05). Conclusion: Based on the results of this study, it can be concluded that etch and one step adhesive in the fissure sealant no significant difference to reduce of microleakage in study in vitro.

Download Full-text

Coronal Sealing Capacity of Temporary Restorative Materials in Pediatric Dentistry: A Comparative Study

International Journal of Clinical Pediatric Dentistry ◽

10.5005/jp-journals-10005-1419 ◽

2017 ◽

Vol 10 (2) ◽

pp. 115-118

Author(s):

Shabnam Milani ◽

Bahman Seraj ◽

Alireza Heidari ◽

Atousa Mirdamadi ◽

Mahdi Shahrabi

Keyword(s):

Comparative Study ◽

Time Lapse ◽

Pediatric Dentistry ◽

Blue Dye ◽

Time Intervals ◽

Dye Penetration ◽

Sealing Capacity ◽

Significant Difference ◽

Restorative Materials

ABSTRACT Aims The aim of this in vitro study is to compare the coronal microleakage of three common temporary restorative materials, namely Coltosol, Compoglass, and Zonalin, used in pediatric dentistry after endodontic treatment at different time intervals (1 week, 1, and 2 months) using dye penetration. Materials and methods Access cavities were prepared in 72 intact extracted premolar teeth. The samples were divided into three groups (n = 24) and filled with Coltosol, Compoglass, or Zonalin. After thermal cycling for 500 cycles (5–55°C), the teeth were immersed in 1% methylene blue dye at 37°C for 1 week (n = 8), 1 month (n = 8), and 2 months (n = 8). The samples were sectioned buccolingually, and the linear depth of dye penetration was measured using a stereomicroscope at 16 × magnification. The data were analyzed using Kruskal–Wallis test. Results There were no significant differences in the microleakage values of Coltosol and Zonalin or Zonalin and Compoglass groups at 1 week (p > 0.05) or 1 month (p > 0.05) intervals, but a significant difference was noted between Coltosol and Compoglass groups (p < 0.01); Coltosol provided a more favorable coronal seal. No significant difference was found among the experimental groups at the 2-month interval (p > 0.05). Conclusion At 1 week or 1 month of use, Coltosol showed better coronal seal. At 2 months, there was no significant difference apparent between the groups. A longer time lapse was associated with an increased likelihood of microleakage. How to cite this article Milani S, Seraj B, Heidari A, Mirdamadi A, Shahrabi M. Coronal Sealing Capacity of Temporary Restorative Materials in Pediatric Dentistry: A Comparative Study. Int J Clin Pediatr Dent 2017;10(2):115-118.

Download Full-text

Longitudinal photographic observation of the occurrence of bubbles in pit and fissure sealants

Journal of Applied Oral Science ◽

10.1590/s1678-77572006000100006 ◽

2006 ◽

Vol 14 (1) ◽

pp. 27-32 ◽

Cited By ~ 2

Author(s):

Renato Herman Sundfeld ◽

Theodore P. Croll ◽

Silvio José Mauro ◽

André Luiz Fraga Briso ◽

Rodrigo Sversut de Alexandre ◽

...

Keyword(s):

Separate Analysis ◽

Fissure Sealants ◽

Pit And Fissure Sealants ◽

Longitudinal Investigation ◽

Sealing Material ◽

Occlusal Surfaces ◽

Significant Difference ◽

Clinical Purpose ◽

Pits And Fissures ◽

Sealing Materials

The purpose of this study was to employ photographic evaluation to observe the presence or absence of bubbles in pit and fissure sealants Concise and Prisma Shield, at 7 days, 18 months, 36 months and 11 years after accomplishment of treatment. METHODS: A hematoxylin-based staining solution was applied to the occlusal surface at the study periods, which allowed assessment of the sealing material on the surface. All occlusal surfaces were photographed and the photographs corresponding to each period were subjected to photographic evaluation. RESULTS: There was statistically significant difference only at 18 months, with a significantly higher number of specimens with bubbles for the Prisma Shield sealant compared to the Concise, whereas the groups were similar at all other study periods. On the other hand, separate analysis of subsequent periods of the sealants did not reveal any significant difference. CONCLUSIONS: According to the results of the present longitudinal investigation, it may be concluded that the sealing materials showed that bubbles came into and went out with time. However, this fact did not interfere with its clinical purpose, provided the remaining material covers the expected region of pits and fissures.

Download Full-text

The impact of occlusal morphology on fissure sealant penetration

Stomatoloski glasnik Srbije ◽

10.2298/sgs0602087p ◽

2006 ◽

Vol 53 (2) ◽

pp. 87-94

Author(s):

Bojan Petrovic ◽

Dejan Markovic ◽

Duska Blagojevic

Keyword(s):

Glass Ionomer ◽

Fissure Sealants ◽

Depth Of Penetration ◽

Fissure Sealant ◽

Group I ◽

Significant Difference ◽

Penetration Ability ◽

The Impact ◽

Pits And Fissures

Introduction: In contemporary preventive dentistry there are numerous experimental in vitro and clinical studies on the growing number of materials for sealing pits and fissures. Aim: the aim of this study was to evaluate the impact of occlusal tooth morphology on penetrating abilities of two types of fissure sealants and measure the unfilled space at the bottom of occlusal groove using scanning electron microscopy (SEM). Materials and methods: The sample consisted of 10 freshly extraced intact premolars and 10 molars randomly divided into 4 groups. In group I (5 premolars), and group II (5 molars) glass ionomer Fuji VII was used as fissure sealant, while in groups III (5 premolars) and IV (5 molars) resin-based sealant (Helioseal-F) was used. Six sections of each tooth were prepared for SEM. Sealant penetration was observed at 30x magnification and the unfilled space at the bottom of the groove at 500x. Sealant penetration in relation to the absolute depth of occlusal grooves was recorded and the unfilled space beneath the sealant was expressed through its greatest diameter. Results were statistically analyzed using ?2 test. Results: There was no statistically significant difference in penetration ability and the unfilled space between tested materials or teeth groups. The average depth of penetration for glass ionomer was 83% and for resin-based sealant 81% of fissure depth. Mean diameters of the unfilled space beneath the sealants were 95 ?m (glass ionomer) and 93 ?m (resin-based sealant). Shallower penetration of both sealing materials was observed in deep pits and fissures with greater diameter of unfilled space (p<0.05). Conclusion: Based on results of the present study it can be concluded that penetration of contemporary fissure sealants largely depends on occlusal tooth anatomy.

Download Full-text

Comparative study of coronal sealing ability using root cross sections method

Stomatoloski glasnik Srbije ◽

10.2298/sgs0704224m ◽

2007 ◽

Vol 54 (4) ◽

pp. 224-230

Author(s):

Dejan Markovic ◽

Vesna Zivojinovic ◽

Slavoljub Zivkovic

Keyword(s):

Cross Sections ◽

Root Canal ◽

Smear Layer ◽

Blue Dye ◽

Human Teeth ◽

Root Canals ◽

Dye Penetration ◽

Gutta Percha ◽

Significant Difference

Objective: An in vitro comparison of coronal microleakage of three root canal sealers was performed using the dye leakage method. Methodology: Ninety-eight single-rooted human teeth were instrumented and randomly allocated to two groups. In the first group, root canals were treated with EDTA to remove the smear layer. The roots in both groups were obturated using laterally condensed gutta-percha and Sealapex, AH-26 or Ketac-Endo as the sealer. The teeth were suspended in 1% methylene blue dye for 6 days. The teeth were then rinsed, sectioned perpendicular to the long axis and evaluated for coronal dye penetration. Results: In general, Ketac-Endo root canal sealer showed greater dye penetration than Sealapex and AH-26. A statistically significant difference in the coronal penetration of the dye was found in the group with intact smear layer. Conclusion: Highly significant differences between AH-26 and Ketac-Endo (p < 0.001) and Sealapex and Ketac Endo (p < 0.001) were found.

Download Full-text

An In vitro Comparative Evaluation of Microleakage of ACP Containing Pit and Fissure Sealant and Moisture Tolerant Pit and Fissure Sealant

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i38a32086 ◽

2021 ◽

pp. 274-280

Author(s):

Rachna Mulani ◽

Aditi Mathur

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

In Vivo Studies ◽

Blue Dye ◽

Fissure Sealants ◽

Fissure Sealant ◽

Pit And Fissure Sealants ◽

Occlusal Surfaces ◽

Pit And Fissure Sealant

Background: Pits and fissure sealants are widely used to prevent caries in children. Microleakage is one of the most crucial factors resulting in sealant compromise. Factors pertaining to microleakage like, pre-treatment of occlusal surfaces, moisture control, bonding systems, and flowability of the sealant, will determine the longevity of the treatment. In vitro microleakage studies are useful in predicting the marginal sealing ability of pit and fissure sealants. Aim: This in vitro study was undertaken to evaluate and compare the microleakage of ACP (Aegis®) containing pit and fissure sealant and moisture tolerant pit and fissure sealant (EmbraceTM WetbondTM). Study Design: In vitro study. Place and Duration of Study: Department of Pedodontics and Preventive Dentistry, Dr. D.Y. Patil Dental College and Hospital, Pune. between June 2020 and February 2021. Methodology: 26 permanent non- carious premolars extracted for orthodontic treatment were used. The sealants used for the analysis of microleakage were Aegis® and EmbraceTM WetbondTM pit and fissure sealants. The premolars were divided into two groups of 13 each. After sealant application, they were immersed in methylene blue dye. The teeth were subjected to thermocycling for 24 hours, after which The teeth were sectioned buccolingually and dye penetration was studied under a stereomicroscope (magnification 10X). Results: Both the groups were studied and statistically evaluated using Mann- Whitney U test. Aegis® showed higher microleakage in (46.2%) than Embrace (38%), though the results were not statistically significant. Conclusion: Both the materials showed microleakage, more In-vivo studies with longer follow- up periods are needed to evaluate and compare the clinical success of these pit & fissure sealants.

Download Full-text

An in vitro study of microleakage of pit and fissure sealants in the presence of occlusal forces

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.24.4.w725246855722u72 ◽

2000 ◽

Vol 24 (4) ◽

pp. 273-278 ◽

Cited By ~ 1

Author(s):

Christina Zervou ◽

Eileen Doherty ◽

Athanasios Zavras ◽

George White

Keyword(s):

Cross Section ◽

In Vitro Study ◽

Fissure Sealants ◽

Pit And Fissure Sealants ◽

Dye Penetration ◽

Wisdom Teeth ◽

Group A ◽

Group B ◽

Group D

The purpose of this study was to evaluate the effect of load on microleakage of pit and fissure sealants. In the first phase of the study load of maximal force (F = 880 N) on central and peripheral enamel was applied. Three groups of ten specimens were tested for microleakage. Group A no application of load, Group B application of load on the central pit of the tooth, and Group application of load on the peripheral enamel 2.5 mm away from the margin of the tooth, at the level of dentinoenamel junction. The results of that study showed significant increase of microleakage on the peripheral sealed enamel, as well as enamel microcracks. So, in the phase II sixty wisdom teeth were sealed with Ultra seal XT plus and load of different magnitude was applied on the peripheral enamel. The specimens were thermocycled for 500 cycles at 5°C and 55°C with a dwell of 30 seconds at each temperature prior to load application. Six groups of ten specimens were tested for microleakage under load. The force was applied at the peripheral enamel, 2.5 mm away from the margin of the tooth, at the level of dentin-enamel junction in cross-section view. Group A: F = 0 N; Group B: F = 300 N; Group C: F = 400 N; Group D: F = 500 N; Group E: F = 600 N; Group F: F = 700 N. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underlying fissure. A linear regression statistics was used with R-squared of 0.31. The groups with load applied (B, C, D, E, F) showed statistically significant results (p<0.05) compared with group A (F = 0 N). Both, the magnitude of the force and tooth morphology may influence the degree of microleakage observed after the placement of sealants.

Download Full-text

Enameloplasty effects on microleakage of pit and fissure sealants under load: an in vitro study

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.24.4.e491n06064j85285 ◽

2000 ◽

Vol 24 (4) ◽

pp. 279-285 ◽

Cited By ~ 5

Author(s):

Christina Zervou ◽

Gerald Kugel ◽

Cataldo Leone ◽

Athanasios Zavras ◽

Eileen Doherty ◽

...

Keyword(s):

In Vitro Study ◽

Conventional Technique ◽

Control Group ◽

Fissure Sealants ◽

Occlusal Force ◽

Pit And Fissure Sealants ◽

Significant Difference ◽

Group A ◽

Group D

Enameloplasty has been implicated in the successful application of pit and fissure sealants. The present study aimed to evaluate the effect of enameloplasty technique on microleakage of sealants when occlusal force was applied on the teeth. The study also allowed a direct comparison of the effectiveness of enameloplasty, when two different burs were used. Six groups of ten human extracted wisdom teeth were tested. Group A: no load, conventional (no enameloplasty - control); Group B: no load, enameloplasty 1/2 round bur (Brasseler USA); Group C: no load, enameloplasty diamond fissure bur REF/UP 791( Ultradent); Group D: load 500 N, conventional , Group E: load 500 N, 1/2 round bur; Group F: load 500 N, diamond fissure bur. Specimens were thermocycled for 500 cycles at 5° and 55° with a dwell time of 30 seconds after load application. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underling fissure. The Kruskal-Wallis One Way Anova and Mann -Whitney U test showed group D having the greatest degree of microleakage statistically significant (p<0.05) comparing with other groups. Sealants prepared with the conventional technique (A, D) had statistically significant differences in microleakage with those prepared with enameloplasty (B,C,E, F) (p = 0.01). Enameloplasty in groups with no load (B, C) did reduce microleakage, but not significantly (p = 0.3) However, loaded teeth with enameloplasty appeared to perform superior, as compared to those without (p=0.005) Regarding the effect of load, teeth without load (Groups A,B, C) were found to have significantly less microleakage than teeth where mechanical force had been applied (Groups D, E, F), (p = 0.01). Microleakage in Group A (conventional technique without load) was significantly less than microleakage in Group D (conventional technique with load), (p = 0.04). However, load did not seem to influence microleakage when enameloplasty had been performed. This finding was true for both round bur enameloplasty (p = 0.29), and fissure bur enameloplasty (p = 0.26). There was no statistically significant difference between Groups B, E (round bur) and C, F (fissure bur) (p > 0.05), or between Groups B and C (p > 0.05) and between Groups E and F (p > 0.05). The results of the study indicated that enameloplasty reduced microleakage of pit and fissure sealants, especially when load was applied to teeth, irrespective of what bur was used to enlarge the fissure, as there was no statistical significant differences between the round and fissured diamond burs. The application of occlusal force to the tooth produces significantly more microleakage, unless enameloplasty is performed.

Download Full-text