scholarly journals Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus

2006 ◽  
Vol 17 (2) ◽  
pp. 134-138 ◽  
Author(s):  
Carlos Estrela ◽  
Cyntia R.A. Estrela ◽  
Daniel de Almeida Decurcio ◽  
Julio Almeida Silva ◽  
Lili Luschke Bammann
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Growth ◽  
Culture Medium ◽  
Distilled Water ◽  
Ultrasonic Cleaning ◽  
Antimicrobial Potential ◽  
Cleaning System ◽  
Colony Forming Units ◽  
A Current ◽  
Made In

The aim of this study was to evaluate the antimicrobial potential of ozone applied to 3 different solutions in an ultrasonic cleaning system against Staphylococcus aureus. A total of 120 mL of S. aureus were mixed in 6 L of the experimental solutions (sterilie distilled water, vinegar and sterile distilled water + Endozime AWpluz) used in a ultrasonic cleaning system (UCS). Ozone was produced by an electric discharge through a current of oxygen and bubbling with flow rate at 7g/h ozone (1.2%) into the microbial suspensions. Ten mL of each experimental suspension were collected and 5 fold dilutions were made in 9 mL of BHI and incubated at 37°C for 48 h. Bacterial growth was evaluated by turbidity of the culture medium. At the same time, 1 mL of bacterial samples was collected and inoculated in BHIA plates. After incubation at 37°C for 48 h, the number of colony forming units (cfu) per mL on BHIA surface was counted. In dilution test in BHI tubes and in BHIA plates (cfu/mL), bacterial growth was not observed in any of the experimental solutions when ozone was added. Under the tested conditions, it may be concluded that the addition of ozone to a ultrasonic cleaning system containing different experimental solutions resulted in antibacterial activity against S. aureus.

scholarly journals Detecção de agentes microbianos das mãos de acadêmicos e docentes de uma instituição de ensino superior em Primavera de Leste – MT

2021 ◽  
Vol 4 (1) ◽  
pp. 105-111
Author(s):  
Vivian Tallita Pinheiro de Santana ◽  
◽  
Phelipe Magalhães Duarte ◽  
Uvleique Alves Fernandes ◽  
Alysson Dias Dalmas ◽  
...  
Keyword(s):  
Higher Education ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Growth ◽  
Culture Medium ◽  
Education Institution ◽  
Higher Education Institution ◽  
Nutrient Culture ◽  
Means Of Transmission

The objective of the present study was to identify the microbiota present in the hands of academics and teachers of a Higher Education Institution in the municipality of Primavera do Leste - MT. Samples were collected from 24 volunteers using a swab. Of the samples collected, 19 (79.1%) showed bacterial growth in a nutrient culture medium, being isolated from this: Staphylococcus aureus (31.6%), S. lugdunensis (31.6%), S. epidermidis (15.8%), S. spp. (5.8%) and Escherichia coli (5.2%). The occurrence of bacteria found in the hands of the volunteers makes it possible to infer that the hands can act as a means of transmission and microbiological multiplication. To avoid this transmission, hygiene and antisepsis measures should be adopted regularly.

scholarly journals A study of the type of antimicrobial action of novel compounds synthesized from substituted benzaminoindoles

2019 ◽  
pp. 57-64
Author(s):  
IS Stepanenko ◽  
SA Yamashkin ◽  
YuA Kostina ◽  
ED Slastnikov ◽  
AA Batarsheva
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Optical Density ◽  
Bacterial Growth ◽  
Culture Medium ◽  
Inhibitory Concentration ◽  
Antimicrobial Action ◽  
Bacteriostatic Effect ◽  
Minimum Inhibitory Concentrations

The antimicrobial activity of novel compounds is tested by determining the minimum inhibitory concentration of the agent in question and investigating a few other parameters, including the type of antimicrobial action the drug exhibits. The aim of this study was to determine the type of antimicrobial action of the compounds synthesized from the substituted benzaminoindoles. The strain of Staphylococcus aureus АТСС 6538-Р was briefly exposed to the compounds with laboratory codes 5D, 7D, HD, and S3. Bacterial growth was evaluated macroscopically under transmitted light. Additionally, photoelectric colorimetry was applied to monitor changes in the optical density of the culture medium. The minimum inhibitory concentrations of the studied compounds delayed bacterial growth for 2–3 days and had a bacteriostatic effect on S. aureus.

scholarly journals In vitro assay on homeopathic solutions on Metarhizium anisopliae (Metsch) Sorok (Ascomycota: Clavicipitaceae)

2016 ◽  
Vol 82 (0) ◽  
Author(s):  
Silvana Damin ◽  
Luis Francisco Angeli Alves ◽  
Andreia Kusumota Bonini ◽  
Talita Moretto Alexandre
Keyword(s):  
Spodoptera Frugiperda ◽  
Metarhizium Anisopliae ◽  
Culture Medium ◽  
In Vitro Assay ◽  
Distilled Water ◽  
Biological Parameters ◽  
Vitro Assay ◽  
Colony Forming Units ◽  
Vitro Effect

ABSTRACT: This study aimed to evaluate the in vitro effect of homeopathic solutions Arsenicum Album 24CH; Calcarea carbonica 30CH; Kali iodatum 100CH;Phosphorus 3CH; Silicea 30CH;Staphysagria 6, 30 and 100CH; Spodoptera frugiperda 30CH; Sulphur 100 and 200CH andThuya occidentalis 200CH on biological parameters of the fungus Metarhizium anisopliae . The solutions were diluted in sterile distilled water (0.1%) and were sprayed on the previously inoculated fungus on PDA culture medium. Germination, colony forming units, vegetative growth, conidial production and insecticidal activity of the fungus against larvae of Diatraea saccharalis (Lepidoptera: Crambidae) were evaluated. Homeopathic solutions did not affect negatively the parameters evaluated. Thus, all treatments were considered compatible to the fungusM. anisopliae.

scholarly journals Interaction of insecticide and disinfectants used in a poultry house with the nematode entomopathogenic Steinernema arenarium

2020 ◽  
Vol 9 (8) ◽  
Author(s):  
Viviane Sandra Alves ◽  
Cristhiane Rohde ◽  
Luis Francisco Angeli Alves
Keyword(s):  
Culture Medium ◽  
Tenebrio Molitor ◽  
Control Treatment ◽  
Distilled Water ◽  
Comparison Standard ◽  
Poultry House ◽  
Poultry Houses ◽  
Colony Forming Units

This study evaluated the effect of cypermethrin insecticide and disinfectants, both used in the management of poultry houses, on nematode Steinernema arenarium, as well as their effect on the bacterium symbiont of nematode. The IOBC/WPRS methodology proposed by Vainio (1992) was used to study the effect on the nematode. The solution of the product was mixed with 1ml of the nematode suspension in distilled water [2000 infective juveniles (IJs)/ml]. As control treatment, only the suspension of the nematode was used. After 48 hours, the viability and the infectivity of the nematode on the larvae of Tenebrio molitor were assayed. The effect of products on the symbiont bacterium was also evaluated. Suspension with 108 CFU (colony forming units)/mL were inoculated in culture medium and three paper discs added (control treatment – distilled water, comparison standard – neomycin, and a disc treated with each product) and the growth was evaluated after 24 hours. All the products reduced the viability of S. arenarium, although only the chlorine-based disinfectant caused reduction above 55%. For the infectivity, the chlorine-based product and the insecticide cipermil reduced in 100% this activity and were considered harmful based on E%. All the products affected the bacterial growing.

scholarly journals Inhibition of bacterial growth by different mixtures of propofol and thiopentone

2005 ◽  
Vol 76 (2) ◽  
Author(s):  
K.E. Joubert ◽  
J. Picard ◽  
M. Sethusa
Keyword(s):  
Escherichia Coli ◽  
Bacterial Growth ◽  
Culture Medium ◽  
Saline Solution ◽  
Bacterial Count ◽  
Yeast Culture ◽  
Wound Infections ◽  
Colony Forming Units ◽  
Bactericidal Properties ◽  
C 50

Propofol is, as a result of its formulation, an ideal bacterial and yeast culture medium. An outbreak of sepsis in humans and an increase in wound infections in dogs has been ascribed to the use of propofol. It has been previously reported that a 1:1 mixture of propofol and thiopentone has bactericidal properties. This study was undertaken to determine if further serial mixtures of propofol and thiopentone maintained the bactericidal properties. Mixtures of 1:1 (solution A), 5:1 (solution B), 10:1 (solution C), 50:1 (solution D) and 100:1 (solution E) of 1 % propofol to 2.5 % thiopentone, 2.5 % thiopentone (solution T), 1 % propofol (solution P) and saline (solution S) were prepared and inoculated with between 105 and 106 colony-forming units of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. A sample was withdrawn from each solution at 0, 1, 6, 12, 48 and 120 hours after inoculation and a bacterial count was performed. This study showed that thiopentone and solution A behaved in similar fashion by inhibiting bacterial growth and was bactericidal after 48 hours. Solution B was not bactericidal against S. aureus and C. albicans. Propofol and solutions D and E all supported growth of all the organisms tested. These data indicate that mixtures of propofol and thiopentone at a ratio less than 1:1 do not maintain the bactericidal properties.

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

Penggunaan Limbah Logam Tembaga yang Didaur Ulang untuk Antibakteri dan Degradasi Metil Merah Secara Fotolisis

2019 ◽  
Vol 4 (1) ◽  
pp. 15
Author(s):  
Ariyetti Ariyetti ◽  
Muhammad Nasir ◽  
Safni Safni ◽  
Syukri Darajat
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nitrate ◽  
Copper Sulfate ◽  
Bacterial Growth ◽  
Methyl Red ◽  
Copper Metal ◽  
Sulfate Hydrate ◽  
Red Dye ◽  
And Staphylococcus Aureus

<p><em>Metil merah merupakan salah satu zat warna golongan azo yang sering digunakan dalam industri dan laboratorium. Penggunaan metil merah dapat menimbulkan efek terhadap kesehatan dan lingkungan. Oleh sebab itu dilakukan metode fotodegradasi dengan menggunakan semikonduktor dan radiasi sinar tampak. Semikonduktor yang digunakan yaitu berbahan dasar tembaga sulfat hidrat dan perak nitrat. Prekusor tembaga sulfat hidrat dibuat dari pengolahan limbah logam tembaga hasil pemotongan tembaga yang ada di bengkel Lembaga Ilmu Pengetahuan Indonesia (LIPI) Bandung. Bahan semikonduktor juga memiliki kemampuan dalam menghambat pertumbuhan bakteri. Hasil optimum yang didapatkan dalam proses fotodegradasi dan antibakteri merupakan gabungan antara kedua prekusor tembaga sulfat hidrat dan perak nitrat dengan bantuan penyinaran. Kemampuan dalam menghambat pertumbuhan bakteri didapatkan persentase kematian 100 % untuk masing-masing bakteri, yaitu Escherichia coli dan Staphylococcus aureus. Aktifitas fotokatalitiknya dengan konsentrasi semikonduktor 10 ppm untuk mendegradasi zat warna metil merah 5 ppm, selama 23 jam, dimana persentase degradasi yang didapatkan dengan penyinaran lebih tinggi dibandingkan dengan tanpa penyinaran. Pengaruh pH larutan terhadap degradasi metil merah yaitu optimum pada pH 12 (basa).</em></p><p><em><br /></em></p><p><em>Methyl red is one of the azo group dyes that is often used in industry and laboratories. The use of methyl red can have an effect on health and the environment. Therefore photodegradation method is done by using semiconductor and visible light radiation. The semiconductor used is based on copper sulfate hydrate and silver nitrate. The copper sulphate hydrate precursor is made from the processing of copper-cut copper metal waste in the workshop of the Indonesian Institute of Sciences (LIPI) in Bandung. Semiconductor materials also have the ability to inhibit bacterial growth. The optimum results obtained in the photodegradation and antibacterial process are a combination of both copper sulfate hydrate precursor and silver nitrate with the help of irradiation. The ability to inhibit bacterial growth obtained 100% mortality for each bacterium, namely Escherichia coli and Staphylococcus aureus. Photocatalytic activity with 10 ppm semiconductor concentration to degrade methyl red dye 5 ppm, for 23 hours, where the percentage of degradation obtained by irradiation is higher than without irradiation. The effect of pH of the solution on the degradation of methyl red is optimum at pH 12 (base).</em></p>

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from &lt;3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

scholarly journals Nanobubbles activate anaerobic growth and metabolism of Pseudomonas aeruginosa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miu Ito ◽  
Yuichi Sugai
Keyword(s):  
Carbon Dioxide ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Growth ◽  
Anaerobic Condition ◽  
Culture Medium ◽  
Cell Concentration ◽  
Essential Elements ◽  
Anaerobic Growth ◽  
Ferrous Ions ◽  
Positively Charged

AbstractThe effect of nanobubbles on anaerobic growth and metabolism of Pseudomonas aeruginosa was investigated. P. aeruginosa grew earlier in the culture medium containing nanobubbles and the bacterial cell concentration in that culture medium was increased a few times higher compared to the medium without nanobubbles under anaerobic condition. Both gas and protein, which are the metabolites of P. aeruginosa, were remarkably produced in the culture medium containing nanobubbles whereas those metabolites were little detected in the medium without nanobubbles, indicating nanobubbles activated anaerobic growth and metabolism of P. aeruginosa. The carbon dioxide nanobubbles came to be positively charged by adsorbing cations and delivered ferrous ions, one of the trace essential elements for bacterial growth, to the microbial cells, which activated the growth and metabolism of P. aeruginosa. The oxygen nanobubbles activated the activities of P. aeruginosa as an oxygen source.

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