scholarly journals Optimizing a culture medium for biomass and phenolic compounds production using Ganoderma lucidum

2013 ◽  
Vol 44 (1) ◽  
pp. 215-223 ◽  
Author(s):  
Carlos Andrés Zárate-Chaves ◽  
María Camila Romero-Rodríguez ◽  
Fabián Camilo Niño-Arias ◽  
Jorge Robles-Camargo ◽  
Melva Linares-Linares ◽  
...  
2014 ◽  
Vol 111 ◽  
pp. 936-946 ◽  
Author(s):  
Irene Fraga ◽  
João Coutinho ◽  
Rui M. Bezerra ◽  
Albino A. Dias ◽  
Guilhermina Marques ◽  
...  

2014 ◽  
Vol 62 (1-2) ◽  
pp. 43-45 ◽  
Author(s):  
Barbara Dohnal ◽  
Wanda Kisiel

The addition of an aqueous extract from fruitbodies of <i>Tylopilus felleus</i> to tissue cultures of <i>Holarrhena antidysenterica</i> (<i>Apocynaceae</i>) caused the accumulation of an unknown compound in the culture medium. The compound was isolated and identified as 5-hydroxymethyl-2-furancarboxaldehyde (1). Moreover, biosynthesis of phenolic compounds was stimulated in response to the stress agents of the fungal preparation. Methyl ferulate (2) was the major phenolic constituent.


1973 ◽  
Vol 51 (4) ◽  
pp. 820-823 ◽  
Author(s):  
Sally Liau ◽  
R. K. Ibrahim

Flax cotyledonary tissue loses its biosynthetic potential for flavonoid and sinapoyl compounds when grown on agar medium containing sucrose, NAA, kinetin, and coconut water. The pattern of phenolic compounds of the callus tissue, however, exhibits a marked differentiation which is related to the levels of growth substances or phenolic precursors added to the culture medium. The induced formation of a C-glycoflavone (iso-orientin) and an anthocyanin (cyanidin-3,5-diglucoside) under high levels of kinetin and white light was demonstrated.


2016 ◽  
Vol 19 (2) ◽  
pp. 33-43
Author(s):  
Suong Thi Tuyet Ha ◽  
Mai Thi Bach Vo

Under the influence of plant hormones, after 8 weeks of in vitro culture, the growth of breadfruit shoot (Artocarpus altilis (Park.) Fosberg) was very different. With shoots that were cultured on 1 mg/L BA medium after 10 days of being transferred to ½ MS supplemented with 10 mg/L BA medium, the percentage of shoot development was the highest (86.8 %), and the secretion of phenolic compounds or forming callus were not observed. On the ½ MS supplemented with 12 mg/L BA medium, shoots growed strongly and healthily but the secretion of phenolic compounds and the forming of callus affected the ability of the shoot development. On the ½ MS supplemented with 0.45 mg/L BA, 0.6 mg/L Kinetin (Kin) medium and on the ½ MS supplemented with 0.45 mg/L BA, 0.6 mg/L Kin, 0.35 mg/L GA3 medium, the percentage of shoot developed were lower than those on the ½ MS supplemented with 10 mg/L medium. The addition of 0.35 mg/L GA3 in the culture medium help to appear the lateral more than the remaining experiments. Roles of respiration rate and endogenous hormones were discussed to understand the physiological changes in the in vitro culture shoots breadfruit.


Author(s):  
NEERAN OBIED JASIM ◽  
NOOR DAKEL MEHDI

Objective: A new medium was prepared to isolate and diagnose the yeast Cryptococcus neoformans from flower and leaves aqueous extract of chrysanthemum. Methods: Prepared differential culture medium for C. neoformans from aqueous extract of leaves and flowers of Chrysanthemum cinerariaefolium and chemical, spectral tests of the extracts were tested, in addition of gas chromatography (GC)–mass was used to diagnose phenolic compounds in both leaves and flowers. Results: Showed that the yeast was grow with typical colonies on the new medium compared with other media which using in diagnosed of this yeast such as Staib agar and Sabourauds dextrose agar and unlike the yeast Candida albicans (as a negative control), which appeared in cream to white on this medium. Furthermore, the colonies are dark brown in color on flower chrysanthemum medium and light brown color on leaves chrysanthemum medium. In addition, the results of the chemical and spectral tests of the extracts confirmed that the plant contains many active compounds such as alkaloids, turbines, tannins, and phenols. The analysis of the extracts of phenolic compounds using GC–mass led to the diagnosis of five compounds in the leaf extract and nine compounds in the flower extract of this plant. Conclusions: The media was prepared is differential medium that use to diagnosis of Cryptococcus such as Staib agar. Moreover, low economic cost, which consists of leaves and flowers of a plant available, abundance and the method of preparation is very simple.


2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S197-S199 ◽  
Author(s):  
R. Fabiani ◽  
P. Rosignoli ◽  
R. Fuccelli ◽  
F. Pieravanti ◽  
A. De Bartolomeo ◽  
...  

In the present investigation the ability of different phenolic compounds, either present or not in olive oil, to induce both apoptosis on tumour cells and H<sub>2</sub>O<sub>2</sub> accumulation in cell culture medium was assesed. Among the phenols studied we found that tyrosol (<I>p</I>-HPEA), homovanillic alcohol and protocatechuic, <I>o</I>-coumaric, vanillic, homovanillic, ferulic and syringic acids did not induce either apoptosis on HL60 cells or H<sub>2</sub>O<sub>2</sub> accumulation, while hydroxytyrosol (3,4-DHPEA), 3,4-dihydroxyphenylacetic acid (3,4-DHPA), 3,4-dihydroxy-hydrocinnamic acid (3,4-DHHC) and gallic acid induced both apoptosis and accumulation of H<sub>2</sub>O<sub>2</sub> in the culture medium which were significantly reduced by catalase. In contrast, the dialdehydic form of elenoic acid linked to hydroxytyrosol (3,4-DHPEA-EDA) and to tyrosol (<I>p</I>-DHPEA-EDA) induced high level of apoptosis not reduced by catalase. Finally, oleuropein exerted a weak pro-apoptotic effect not mediated by H<sub>2</sub>O<sub>2</sub> release. From these results it is evident that: (<I>I</I>) the cathecol moiety of phenols is necessary but not sufficient to induce apoptosis and H<sub>2</sub>O<sub>2</sub> accumulation; <I>(ii</I>) the 3,4-DHPEA metabolism may partially reduce its pro-apoptotic potential; <I>(iii</I>) the pro-apoptotic activity of 3,4-DHPEA-EDA and<I> p</I>-DHPEA-EDA is not mediated by H<sub>2</sub>O<sub>2</sub> releasing activity.


1978 ◽  
Vol 56 (14) ◽  
pp. 1588-1593 ◽  
Author(s):  
Saeed I. Zafar ◽  
Nicholas Colotelo

Numerous pycnidia of Plenodomus meliloti occur naturally on roots of legumes and cultures incubated in continuous light. Cultures incubated in darkness produced pycnidia only when various inorganic oxidants and phenolic compounds were incorporated into the culture medium, and even then they produced considerably fewer pycnidia than cultures incubated in continuous light did. The relationship between oxidizing compounds and pycnidia initiation is discussed briefly.


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