scholarly journals The effect of a fungal preparation on the tissue cultures of Holarrhena anfidysenterica (Roxb.) Wall.

2014 ◽  
Vol 62 (1-2) ◽  
pp. 43-45 ◽  
Author(s):  
Barbara Dohnal ◽  
Wanda Kisiel
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Culture Medium ◽  
Tissue Cultures ◽  
Unknown Compound ◽  
Holarrhena Antidysenterica ◽  
Phenolic Constituent ◽  
Methyl Ferulate

The addition of an aqueous extract from fruitbodies of <i>Tylopilus felleus</i> to tissue cultures of <i>Holarrhena antidysenterica</i> (<i>Apocynaceae</i>) caused the accumulation of an unknown compound in the culture medium. The compound was isolated and identified as 5-hydroxymethyl-2-furancarboxaldehyde (1). Moreover, biosynthesis of phenolic compounds was stimulated in response to the stress agents of the fungal preparation. Methyl ferulate (2) was the major phenolic constituent.

scholarly journals PREPARATION DIFFERENTIAL CULTURE MEDIUM FOR CRYPTOCOCCUS NEOFORMANS FROM AQUEOUS EXTRACT OF LEAVES AND FLOWERS OF CHRYSANTHEMUM CINERARIAEFOLIUM

2019 ◽  
pp. 114-118
Author(s):  
NEERAN OBIED JASIM ◽  
NOOR DAKEL MEHDI
Keyword(s):  
Phenolic Compounds ◽  
Cryptococcus Neoformans ◽  
Aqueous Extract ◽  
Culture Medium ◽  
Economic Cost ◽  
Negative Control ◽  
The Media ◽  
Differential Medium ◽  
Chrysanthemum Cinerariaefolium ◽  
Light Brown Color

Objective: A new medium was prepared to isolate and diagnose the yeast Cryptococcus neoformans from flower and leaves aqueous extract of chrysanthemum. Methods: Prepared differential culture medium for C. neoformans from aqueous extract of leaves and flowers of Chrysanthemum cinerariaefolium and chemical, spectral tests of the extracts were tested, in addition of gas chromatography (GC)–mass was used to diagnose phenolic compounds in both leaves and flowers. Results: Showed that the yeast was grow with typical colonies on the new medium compared with other media which using in diagnosed of this yeast such as Staib agar and Sabourauds dextrose agar and unlike the yeast Candida albicans (as a negative control), which appeared in cream to white on this medium. Furthermore, the colonies are dark brown in color on flower chrysanthemum medium and light brown color on leaves chrysanthemum medium. In addition, the results of the chemical and spectral tests of the extracts confirmed that the plant contains many active compounds such as alkaloids, turbines, tannins, and phenols. The analysis of the extracts of phenolic compounds using GC–mass led to the diagnosis of five compounds in the leaf extract and nine compounds in the flower extract of this plant. Conclusions: The media was prepared is differential medium that use to diagnosis of Cryptococcus such as Staib agar. Moreover, low economic cost, which consists of leaves and flowers of a plant available, abundance and the method of preparation is very simple.

scholarly journals Antioxidant Evaluation of the Aqueous Extract of Hulls of Campsiandra laurifolia in Colitis Induced by Acetic Acid in Wistar Rats

2021 ◽  
Vol 41 (02) ◽  
pp. 138-144
Author(s):  
Lucas Petitemberte de Souza ◽  
Henrique Sarubbi Fillmann ◽  
Sandielly Rebeca Benitez da Fonseca ◽  
Marilda da Silva Brasil ◽  
Norma Anair Possa Marroni ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Antioxidant Activities ◽  
Experimental Colitis ◽  
Anal Pressure ◽  
Antioxidant Power ◽  
Significance Level ◽  
Sphincter Anal

AbstractDue to the ethnopharmacological use of Campsiandra laurifolia (Fabaceae), popularly known as Acapurana, to treat wounds and ulcers, associated with the lack of alternative treatments for intestinal inflammations such as ulcerative colitis (UC), the present work sought to characterize its phytochemical and antioxidant activities, and to evaluate remedial action in experimental colitis with acetic acid. Phytochemical analyzes were performed through qualitative and quantitative colorimetric tests of the main secondary metabolites. In the colitis model, 24 male Wistar rats aged ± 60 days old were used, divided into 4 groups: Control (CO) control + aqueous extract of C. laurifolia 50 mg/kg (CO + A50); Colitis (CL); and Colitis + aqueous extract of C. laurifolia 50 mg/kg (CL + A50). Measurement of sphincter anal pressure and histological tests of the large intestine, lipoperoxidation (LPO), enzyme activity of superoxide dismutase (SOD), and levels of glutathione (GSH) were performed. For statistical analysis, the oxidative stress (OS) results were expressed as means ± standard error, adopting a significance level of p < 0.05. The screening indicated the presence of flavonoids, saponins and tannins in the extract, with high levels of phenolic compounds and tannins, and was related to high antioxidant capacity. In the histological analysis, the CL group presented loss of the crypts, edema and inflammatory infiltrate. The use of C. laurifolia extract restructured the crypts, decreased edema and increased sphincter anal pressure, with a decrease in LPO, SOD, and an increase in GSH. It is suggested that the use of C. laurifolia extract reduces OS due to its antioxidant power conferred by the phenolic compounds present in the extract.

scholarly journals Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3303
Author(s):  
Amina M. G. Zedan ◽  
Mohamed I. Sakran ◽  
Omar Bahattab ◽  
Yousef M. Hawsawi ◽  
Osama Al-Amer ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Individual Treatment ◽  
Alcoholic Extract ◽  
Anticancer Effect ◽  
Mcf7 Cells ◽  
Vespa Orientalis ◽  
Anti Inflammatory

The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2; (2) suppression of intracellular reactive oxygen species; (3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1; (4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1; and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.

Hypoglycemic effects of phenolic compound-rich aqueous extract from water dropwort (Oenanthe javanica DC.) on streptozotocin-induced diabetic mice

2020 ◽  
Vol 44 (14) ◽  
pp. 5190-5200
Author(s):  
Shudong He ◽  
Mingming Tang ◽  
Zuoyong Zhang ◽  
Haiyan Liu ◽  
Mingfeng Luo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Aqueous Extract ◽  
Akt Pathway ◽  
Diabetic Mice ◽  
Glut4 Translocation ◽  
Hypoglycemic Action ◽  
Oenanthe Javanica ◽  
Water Dropwort

Phenolic compounds in water dropwort aqueous extract were identified, and the IRS-2/PI3K-AKT pathway and GLUT4 translocation were regulated for hypoglycemic action.

scholarly journals Extract of Mangifera indica L. leaves may reduce biofilms of Staphylococcus spp. in stainless steel and teatcup rubbers

2019 ◽  
Vol 26 (1) ◽  
pp. 11-20
Author(s):  
Andressa GB Manzur ◽  
Valdo SM Junior ◽  
Franciellen Morais-Costa ◽  
Emanuelly GA Mariano ◽  
Roberta T Careli ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Mangifera Indica ◽  
Ethanolic Extract ◽  
Aureus Strain ◽  
Leaf Extracts ◽  
Log Reduction ◽  
Agar Diffusion Test ◽  
Staphylococcus Spp

Human ingestion of antimicrobial residues in dairy products is a problem of public health. In this study we evaluated antibacterial effectiveness of aqueous and ethanolic leaf extracts of Mangifera indica L. as a natural alternative for reduction of Staphylococcus spp. biofilms. Eight Staphylococcus spp. strains from cows with mastitis and the Staphylococcus aureus strain (ATCC 25923) were evaluated. High performance liquid chromatography indicated the presence of phenolic compounds in the aqueous extract. Agilent Technologies gas chromatography revealed the presence of phenolic compounds, carbohydrates, and gallic acid in ethanolic extract. The tannin contents were 2.27 ± 0.01 and 2.67 ± 0.01% for ethanolic extract and aqueous extract, respectively. Extracts were evaluated in agar diffusion test and the size of inhibition zones were compared. The ethanolic extract was the most effective, showing minimum inhibitory concentrations and minimum bactericidal concentrations between 1.8–7.5 and 15.1–45.3 mg/ml, respectively. After tannin removal, the extracts were less effective, indicating these secondary metabolites as one of the main antibacterial compounds. The ethanolic extract at 45.3 mg/ml reduced mature biofilms in teat rubber (average: 3.91 log reduction) and stainless steel (average: 3.87 log reduction) after 5 min of contact. The extracts of M. indica leaves represent natural alternatives against Staphylococcus spp. strains and the ethanolic extract shows potential as a natural sanitizer.

Induction of Phenolic Compounds in Wheat (Triticum aestivum L.) Tissue Cultures by Streptomycin

2007 ◽  
Vol 62 (1-2) ◽  
pp. 50-54 ◽  
Author(s):  
Atiat M. A. Hassan
Keyword(s):  
Triticum Aestivum ◽  
Phenolic Compounds ◽  
Experimental Period ◽  
Triticum Aestivum L ◽  
Stationary Growth Phase ◽  
Total Phenolic ◽  
Ms Medium ◽  
Tissue Cultures ◽  
Mature Embryos ◽  
Pal Activity

The tissue cultures of wheat (Triticum aestivum L.) were induced from the mature embryos (explants) of the dry grains and grown on MS medium containing kinetin (0.1 mg/l) and 2,4 D (1.0 mg/l). The cultures were incubated for two weeks at (25 ± 2) °C under a light/dark regime (16 h light daily). The formed calli were subcultured at the beginning of the stationary growth phase (15 days) with fresh MS medium containing 0, 5, 10, 25, 50, 100, 150 mg/l streptomycin elicitor and maintained for two weeks for three subcultures. A significant increase in phenylalanine ammonia lyase (PAL) activity coincided with the increase of the total phenolic compounds after elicitation with streptomycin. Maximum induction was recorded during the first two weeks, then gradually declined during the rest of the experimental period, but the values attained were still markedly higher than that of the control. The endogenous cinnamic acid content was also increased significantly with the increase in PAL activity making about 2-18% of the total phenolic acids. The growth and accumulation of phenolic compounds were inversely related. However, accumulation of phenolic compounds became limited for growth of wheat tissue culture especially during the long term cultivation.

scholarly journals Optimizing a culture medium for biomass and phenolic compounds production using Ganoderma lucidum

2013 ◽  
Vol 44 (1) ◽  
pp. 215-223 ◽  
Author(s):  
Carlos Andrés Zárate-Chaves ◽  
María Camila Romero-Rodríguez ◽  
Fabián Camilo Niño-Arias ◽  
Jorge Robles-Camargo ◽  
Melva Linares-Linares ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ganoderma Lucidum ◽  
Culture Medium

Biochemical differentiation in flax tissue culture. Phenolic compounds

1973 ◽  
Vol 51 (4) ◽  
pp. 820-823 ◽  
Author(s):  
Sally Liau ◽  
R. K. Ibrahim
Keyword(s):  
Tissue Culture ◽  
Phenolic Compounds ◽  
White Light ◽  
Culture Medium ◽  
Callus Tissue ◽  
Agar Medium ◽  
Coconut Water ◽  
Growth Substances ◽  
Biochemical Differentiation

Flax cotyledonary tissue loses its biosynthetic potential for flavonoid and sinapoyl compounds when grown on agar medium containing sucrose, NAA, kinetin, and coconut water. The pattern of phenolic compounds of the callus tissue, however, exhibits a marked differentiation which is related to the levels of growth substances or phenolic precursors added to the culture medium. The induced formation of a C-glycoflavone (iso-orientin) and an anthocyanin (cyanidin-3,5-diglucoside) under high levels of kinetin and white light was demonstrated.

Phenolic compounds from the aqueous extract ofAcacia catechu

2011 ◽  
Vol 13 (9) ◽  
pp. 826-830 ◽  
Author(s):  
Xing-Cui Li ◽  
Chao Liu ◽  
Li-Xin Yang ◽  
Ruo-Yun Chen
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract
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