scholarly journals Anthelmintic effect of Cymbopogon citratus essential oil and its nanoemulsion on sheep gastrointestinal nematodes

2019 ◽  
Vol 28 (3) ◽  
pp. 522-527 ◽  
Author(s):  
Iara Tersia Freitas Macedo ◽  
Lorena Mayana Beserra de Oliveira ◽  
Weibson Paz Pinheiro André ◽  
José Vilemar de Araújo Filho ◽  
Jéssica Maria Leite dos Santos ◽  
...  
Keyword(s):  
Essential Oil ◽  
Worm Burden ◽  
Anthelmintic Resistance ◽  
Gastrointestinal Nematodes ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
Main Component ◽  
Vitro Effect

Abstract The anthelmintic resistance stimulated the search for strategies for controlling gastrointestinal nematodes, including the use of free essential oils or its nanoemulsion. This study evaluated the anthelmintic efficacy of Cymbopogon citratus essential oil (CcEO) and C. citratus essential oil nanoemulsion (CcEOn). Pysicochemical analyses were performed. The in vitro effect was determined using the egg hatch test (EHT) on Haemonchus contortus and in vivo effect was evaluated in sheep infected with gastrointestinal nematodes. The animals were treated with CcEO (500 mg/kg) or CcEOn (450 mg/kg) for the fecal egg count (FEC) and the determination of worm burden. The main component of CcEO was citral. The CcEO content in the nanoemulsion was 20% (v/v), and the mean particle size was 248 nm. In EHT, CcEO and CcEOn (1.25 mg/mL) inhibited larval hatching by 98.4 and 97.1%, respectively. Three animals treated with CcEO died whereas in the group treated with CcEOn one animal died. The FEC and total worm burden of the treated groups did not differ from the negative control (p>0.05). The CcEOn showed efficacy only on H. contortus (p<0.05). In conclusion, nanoencapsulation reduced toxicity and increased efficacy on H. contortus.

scholarly journals Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.

2017 ◽  
Vol 38 (4) ◽  
pp. 1947 ◽  
Author(s):  
Jessica Nascimento Moraes Monteiro ◽  
Anderson Barros Archanjo ◽  
Gabriela Porfirio Passos ◽  
Adilson Vidal Costa ◽  
Lenir Cardoso Porfirio ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Gastrointestinal Nematodes ◽  
Active Principle ◽  
Chenopodium Ambrosioides ◽  
In Vivo Test ◽  
Vitro Effect

The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces.

scholarly journals In vitro anthelmintic activity of Lippia gracilis Schauer essential oil against egg-hatching of goat gastrointestinal nematodes

2020 ◽  
Vol 87 ◽  
Author(s):  
Anna Lopes da Costa Souza ◽  
Cristina Karine de Oliveira Rebouças ◽  
Cynthia Cavalcanti de Albuquerque ◽  
Cristiane de Carvalho Ferreira Lima Moura ◽  
Taffarel Melo Torres ◽  
...  
Keyword(s):  
Essential Oil ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Effective Concentration ◽  
Alternative Methods ◽  
Drug Resistant ◽  
Egg Hatching ◽  
Hatching Process ◽  
Vitro Effect

ABSTRACT Since drug-resistant nematodes became a common problem in sheep and goat industries, alternative methods using natural products have emerged as a viable and sustainable anthelmintic treatment option. Here, the in vitro effect of essential oil extracted from Lippia gracilis Schauer was assessed on the hatching process of nematodes recovered from naturally infected goats. Essential oil at concentrations of 0.08% (0.008 μL/mL), 0.12% (0.012 μL/mL), and 0.16% (0.016 μL/mL) was able to induce an average inhibition of 74.7, 84 and 93%, respectively. The effective concentration required to inhibit egg hatching in 50% of eggs (EC50) was 0.03452%. Therefore, essential oil of L. gracilis showed promisor in vitro anthelmintic results against egg-hatching of goat gastrointestinal nematodes.

scholarly journals In vitro fungitoxic potential of Lippia gracilis (Schauer) essential oil against phytopathogens

2020 ◽  
pp. 667-674
Author(s):  
Kevison Romulo da Silva França ◽  
Flavia Mota de Figueredo Alves ◽  
Tiago Silva Lima ◽  
Alda Leaby dos Santos Xavier ◽  
Plínio Tércio Medeiros de Azevedo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Fungal Growth ◽  
Fungal Species ◽  
Phytopathogenic Fungi ◽  
Negative Control ◽  
Growth Speed ◽  
Randomized Design

This study evaluates the in vitro effects of Lippia gracilis essential oil on the mycelial growth of phytopathogenic fungi. Experiments were carried out using a completely randomized design to assess the effects of eight treatments. Five replicates were evaluated for each experimental group. The essential oil was incorporated into the potato dextrose culture medium and poured into Petri dishes. Treatments were comprised of different concentrations of the oil (0.0125, 0.025, 0.05, 0.1, and 0.2%), a negative control (0.0%), and two positive controls (commercial fungicides). The plates were inoculated with fungi including Colletotrichum gloeosporioides, C. musae, C. fructicola, C. asianum, Alternaria alternata, A. brassicicola, Fusarium solani, F. oxysporum f. sp. cubense, and Lasiodiplodia theobromae and were incubated for seven days at 27 ± 2°C. The following variables were measured to verify the differences observed among treatments: percentage of mycelial growth inhibition and index of mycelial growth speed. All concentrations of L. gracilis oil inhibited the mycelial growth of the fungal species evaluated. The complete inhibition was observed between concentrations of 0.0125 and 0.1%. Treatment with oil inhibited fungal growth with similar, or even greater, efficiency than commercial fungicides.. We recommend the development of in vivo tests to verify whether L. gracilis essential oil can protect against fungal disease in live plants.

scholarly journals Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

2015 ◽  
Vol 24 (3) ◽  
pp. 268-275 ◽  
Author(s):  
Iara Tersia Freitas Macedo ◽  
Lorena Mayana Beserra de Oliveira ◽  
Wesley Lyeverton Correia Ribeiro ◽  
Jessica Maria Leite dos Santos ◽  
Kaline das Chagas Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Meriones Unguiculatus ◽  
Parasitic Nematodes ◽  
Control Group ◽  
Cymbopogon Citratus ◽  
Egg Hatching

Parasitic nematodes are of major economic importance in livestock. An alternative for the control of parasites is phytotherapy. This study evaluated the efficacy of Cymbopogon citratus decoction (CcD), C. citratus essential oil (CcEo) and citral against Haemonchus contortus using in vitro egg hatch test (EHT) and larval development test (LDT) and an in vivo test using a Meriones unguiculatus (gerbil) model. The effect of 800 mg/kg CcEo was evaluated in gerbils that had been artificially infected with 5,000 third-stage H. contortus larvae. The effective concentrations required to inhibit 50% (EC50) of egg hatching were 0.46, 0.14 and 0.13 mg/mL for CcD, CcEo and citral, respectively. The EC50 values in the LDT were 5.04, 1.92 and 1.37 mg/mL for CcD, CcEo and citral, respectively. H. contortus population in the group treated with C. citratus essential oil was reduced by 38.5% (P< 0.05) in comparison to the control group. These results suggest that it may be possible to use C. citratusessential oil to control of H. contortus parasite of small ruminant.

scholarly journals Evaluation of Eucalyptus citriodora essential oil on goat gastrointestinal nematodes

2011 ◽  
Vol 20 (3) ◽  
pp. 223-227 ◽  
Author(s):  
Iara Tersia Freitas Macedo ◽  
Claudia Maria Leal Bevilaqua ◽  
Lorena Mayana Beserra de Oliveira ◽  
Ana Lourdes Fernandes Camurça-Vasconcelos ◽  
Luiz da Silva Vieira ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Essential Oil ◽  
Larval Development ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Gastrointestinal Parasites ◽  
Eucalyptus Citriodora ◽  
Egg Hatching

Phytotherapy may be an alternative strategy for controlling gastrointestinal parasites. This study evaluated the anthelmintic efficacy of Eucalyptus citriodora essential oil (EcEO). The in vitro effects of EcEO were determined through testing the inhibition of egg hatching and larval development of Haemonchus contortus. EcEO was subjected to acute toxicity testing on mice, orally and intraperitoneally. The in vivo effects of EcEO were determined by the fecal egg count reduction test (FECRT) in goats infected with gastrointestinal nematodes. The results showed that 5.3 mg.mL-1 EcEO inhibited egg hatching by 98.8% and 10.6 mg.mL-1 EcEO inhibited H. contortus larval development by 99.71%. The lethal doses for 50% of the mice were 4153 and 622.8 mg.kg-1, for acute toxicity orally and intraperitoneally. In the FECRT, the efficacy of EcEO and ivermectin was 66.25 and 79.16% respectively, on goat gastrointestinal nematodes eight days after treatment. EcEO showed in vitro and in vivo anthelmintic activity.

scholarly journals Worm-control practices and prevalence of anthelmintic resistance using in vivo FECRTs on smallholder sheep farms in Lithuania

2016 ◽  
Vol 53 (1) ◽  
pp. 24-30 ◽  
Author(s):  
T. Kupčinskas ◽  
I. Stadalienė ◽  
A. Šalomskas ◽  
P. Trusevičius ◽  
M. Varady ◽  
...  
Keyword(s):  
Anthelmintic Resistance ◽  
Gastrointestinal Nematodes ◽  
Parasitic Nematodes ◽  
Future Studies ◽  
Faecal Samples ◽  
Two Samples ◽  
Faecal Egg Count ◽  
Worm Control

SummaryThis study determined the prevalence of anthelmintic resistance (AR) in parasitic nematodes on smallholder sheep farms in Lithuania from April to November 2014. Faecal samples were collected from two groups of 10-15 sheep treated with fenbendazole (FBZ) or ivermectin (IVM) on 18 sheep farms. Two samples were collected from each group: on day zero (T1) and 10-14 days after treatment. Faecal egg counts (eggs per gramme, EPG) were determined using a modified McMaster technique. Animals with < 140 EPG on day zero were removed from the analysis. The prevalence of AR was estimated using the in vivo faecal egg count reduction test. AR to FBZ was detected on three of 15 farms where FBZ was used (20 %) and was suspected on one farm (6.7 %). AR to IVM was detected on two of 16 farms where IVM was used (12.5 %). The main species of resistant gastrointestinal nematodes (GINs) identified after treatment were Teladorsagia spp. and Trichostrongylus spp. A questionnaire surveying 71 sheep farmers estimated that 71.8 % of sheep farmers used anthelmintics against GINs. IVM was the most frequently (68.6 %) applied anthelmintic, and 62.7 % of the respondents reported treating their animals twice a year. This study confirmed the presence of AR to GIN infections on sheep farms in Lithuania. Future studies should assess the prevalence of AR to GIN infection using in vitro methods.

scholarly journals Amoebicidal Activity of Essential Oil ofDysphania ambrosioides(L.) Mosyakin & Clemants in an Amoebic Liver Abscess Hamster Model

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Manuel Enrique Ávila-Blanco ◽  
Martín Gerardo Rodríguez ◽  
José Luis Moreno Duque ◽  
Martin Muñoz-Ortega ◽  
Javier Ventura-Juárez
Keyword(s):  
Essential Oil ◽  
Public Health Problem ◽  
Amoebic Liver Abscess ◽  
Traditional Use ◽  
Hamster Model ◽  
Low Efficiency ◽  
Main Component ◽  
Amoebicidal Activity

Amebiasis is a parasitic disease that extends worldwide and is a public health problem in developing countries. Metronidazole is the drug recommended in the treatment of amebiasis, but its contralateral effects and lack of continuity of treatment induce low efficiency, coupled with the appearance of resistant amoebic strains. Therefore, the search of new compounds with amoebicidal activity is urgent and important. In this study, we evaluated the in vitro and in vivo antiamoebic activity of the essential oilDysphania ambrosioides(L.) Mosyakin & Clemants. It exhibited an IC50= 0.7 mg/mL against trophozoites. The oral administration of essential oil (8 mg/kg and 80 mg/kg) to hamster infected withEntamoeba histolyticareverted the infection. Ascaridole was identified as the main component of essential oil ofD. ambrosioides. The identification of amoebicidal activity of Ascaridole gives support to the traditional use. Further studies with Ascaridole will be carried out to understand the mechanism involved.

scholarly journals Control of Alternaria alternata Using Melaleuca Essential Oil (Melaleuca alternifolia)

2019 ◽  
pp. 1-10
Author(s):  
Flávia Mota de Figuerêdo Alves ◽  
Kevison Romulo da Silva França ◽  
Ionaly Gomes de Araújo ◽  
Lídia Pinheiro da Nóbrega ◽  
Alda Leaby dos Santos Xavier ◽  
...  
Keyword(s):  
Essential Oil ◽  
Alternaria Alternata ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Positive Control ◽  
Negative Control ◽  
Growth Speed ◽  
In Vitro Test

Aims: This study aimed to evaluate the fungitoxic potential of melaleuca essential oil on the mycelial growth of Alternaria alternata under in vitro condition and the treatment of cowpea beans. Study Design: The experiments comprised completely randomized designs: Eleven treatments with five replicates on in vitro test; and six treatments with five replicates on in vivo test. Place and Duration of Study: The work was carried out at the Center for Agrifood Science and Technology of the Federal University of Campina Grande, Pombal, Brazil, since February 2018 to February 2019. Methodology: In the in vitro experiment, the essential oil was incorporated into the culture medium and poured into Petri dishes. The treatments consisted of different concentrations of the essential oil (0.0125, 0.025, 0.05, 0.1, 0.2, 0.25, 0.50, 0.75, and 1.0%), a negative control (0.0%), and a positive control (Thiram). Discs of culture medium with fungal mycelia were inoculated in the center of the plates and incubated for seven days at 27±2ºC. The percentage of mycelial growth inhibition (PGI) and the index of mycelial growth speed (IMGS) was calculated to verify the difference between treatments. In the in vivo experiment, the bean seeds were treated with different concentrations of EO (0.0, 0.2, 0.5, 1.0, and 5.0%), a negative control (0.0%), and positive control (Thiram). Seeds were inoculated with colonies of the fungus for 48 hours, and after that, we performed the seed sanity test. Results: Under in vitro conditions, all concentrations of melaleuca essential oil reduced the mycelial growth of A. alternata. The oil reached complete inhibition of fungal growth from 0.2% concentration and above. In the cowpea treatment, the essential oil had no significant control over the percentage of infected seeds. Conclusion: The melaleuca essential oil had a fungitoxic effect on the A. alternata under in vitro conditions. However, using the adopted methodology, on the cowpea bean seed treatment, the essential oil did not reduce the incidence of A. alternata.

scholarly journals Nanoparticles as Alternatives for the Control of Haemonchus contortus: A Systematic Approach to Unveil New Anti-haemonchiasis Agents

2021 ◽  
Vol 8 ◽  
Author(s):  
Rehman Ali ◽  
Nisar Ahmad ◽  
Sakina Mussarat ◽  
Abdul Majid ◽  
Sultan F. Alnomasy ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Resistance ◽  
Small Ruminants ◽  
Gastrointestinal Nematode ◽  
Future Research ◽  
Cymbopogon Citratus ◽  
Toxicological Effects ◽  
Nano Emulsion

Haemonchus contortus is an infectious gastrointestinal nematode parasite of small ruminants. This study addresses the in vitro/in vivo anti-haemonchiasis potential, toxicological effects, and mechanism of action of nanoparticles. Online databases were used to search and retrieve the published literature (2000 to 2021). A total of 18 articles were selected and reviewed, out of which, 13 (72.2%) studies reported in vitro, 9 (50.0%) in vivo, and 4 (22.2%) both in vitro/in vivo efficacy of different nanoparticles. Mostly, organic nanoparticles (77.7%) were used including polymeric (85.7%) and lipid nanoparticles (14.3%). The highest efficacy, in vitro, of 100% resulted from using encapsulated bromelain against eggs, larvae, and adult worm mortality at 4, 2, and 1 mg/ml, respectively. While in vivo, encapsulated Eucalyptus staigeriana oil reduced worm burden by 83.75% and encapsulated Cymbopogon citratus nano-emulsion by 83.1%. Encapsulated bromelain, encapsulated Eucalyptus staigeriana oil, and encapsulated Cymbopogon citratus nano-emulsion were safe and non-toxic in vivo. Encapsulated bromelain damaged the cuticle, caused paralysis, and death. Nanoparticles could be a potential source for developing novel anthelmintic drugs to overcome the emerging issue of anthelmintic resistance in H. contortus. Studies on molecular effects, toxicological consequences, and different pharmacological targets of nanoparticles are required in future research.

scholarly journals Oxfendazole Resistance in Gastrointestinal Nematodes of Beetal Goats at Livestock Farms of Punjab (Pakistan)

2007 ◽  
Vol 76 (1) ◽  
pp. 79-85 ◽  
Author(s):  
M. Saeed ◽  
Z. Iqbal ◽  
A. Jabbar
Keyword(s):  
Anthelmintic Resistance ◽  
Gastrointestinal Nematodes ◽  
Post Treatment ◽  
Egg Hatch ◽  
Treatment Frequency ◽  
Contributory Factors ◽  
Reduction Test ◽  
Egg Hatch Assay

This study was carried out to screen goat farms for anthelmintic resistance (AR) against oxfendazole (OXF) and to determine contributory factors for its development. For this purpose, Beetal goat farms (n = 18) were randomly selected, with natural mixed gastrointestinal nematodosis infection. In vivo (faecal egg count reduction test) and in vitro (egg hatch assay) tests were used to ascertain the presence of AR while a scorecard was used to determine the role of possible contributory factors for oxfendazole resistance. For in vivo test, the experimental animals were divided into two groups of 10 animals each; one group received OXF treatment, while the other served as control. Pre- and post-treatment coproculture was performed to identify the species and genera of nematodes. Egg hatch assay (EHA) was used to confirm the results of FECRT. Fecal egg count reduction test (FECRT) revealed the development of resistance on six farms and post-treatment larval cultures indicated Haemonchus contortus, Trichostrongylus colubriformis, Cooperia curticei, Teladorsagia circumcincta and Oesophagostomum spp. as dominant species with resistance. Furthermore, EHA confirmed the results of FECRT. Among the presumptive factors for AR, the highest composite score was for rotation of anthelmintics followed by treatment frequency, dose rate and nature of medication. The scorecard for the development of AR, used in this study, may be helpful for the assessment of contributory factors of AR.

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