scholarly journals Chemical constituents and pharmacological profile of Gunnera manicata L. extracts

2014 ◽  
Vol 50 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Kristiane de Cássia Mariotti ◽  
Roselena Silvestri Schuh ◽  
Jéssica de Matos Nunes ◽  
Sabrina Pinto Salamoni ◽  
Gabriela Meirelles ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
South African ◽  
Native Species ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Total Phenolic ◽  
South American ◽  
Flavonoid Content ◽  
Antibacterial And Antifungal ◽  
Diffusion Assay

Gunnera perpensa L. (Gunneraceae) is a native South African plant widely used in traditional medicine as an antibacterial and antifungal. In southern Brazil there is the native species called Gunnera manicata L. that also belongs to the Gunneraceae. Nevertheless, there is no information about chemical and pharmacological properties of South American Gunnera species. Therefore this study aimed at assessing the phytochemical and pharmacological profiles of aqueous and methanol Brazilian G. manicata extracts. The results showed that antimicrobial activity in an agar diffusion assay was effective against Staphylococcus aureus and Candida albicans . Phenolic compounds were investigated by liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS) and all extracts presented gallic acid and only the methanol extract obtained from the leaves exhibited hyperoside. Rutin, quercetin and chlorogenic acid were not found in the samples analysed. Total phenolic content was higher in methanol extract and total flavonoid content was low in all extracts. Antioxidant activity was evaluated by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical test, and all samples presented good to moderate antioxidant activity. These results encourage complementary studies on the chemical composition of the plant extracts focusing on isolation and structure elucidation of their active compounds.

scholarly journals FOURIER-TRANSFORM INFRARED SPECTROSCOPY STUDIES AND EVALUATION OF TOTAL PHENOLICS, FLAVONOIDS, AND ANTIOXIDANT ACTIVITY OF CLEOME GYNANDRA

2019 ◽  
pp. 214-218 ◽  
Author(s):  
RAMYA KUBER BANOTH ◽  
ASHWINI THATIKONDA
Keyword(s):  
Antioxidant Activity ◽  
Colorimetric Assay ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Flavonoid Content ◽  
Cleome Gynandra ◽  
Dpph Radical Scavenging

Objective: The objective of this study was to evaluate the nature of chemical constituents, total phenolics, total flavonoids, and antioxidant activity of Cleome gynandra and their functional groups with the help of phytochemical, Fourier-transform infrared spectroscopy (FTIR) analysis, colorimetric assay, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Methods: C. gynandra of the Cleomaceae family is an annual herb. The dried leaves were powdered and extracted using Soxhlet apparatus by different solvents. Preliminary phytochemical analysis was carried out to identify the phytoconstituents present in the extract of C. gynandra, FTIR spectrum was scanned at the range of 4000-400 cm−1. The extracts were subjected to the colorimetric assay in triplicate manner to quantitative determination of total phenolic and total flavonoid content. Gallic acid and rutin used as standards to determine the total phenolic content and total flavonoid content. Antioxidant activity was evaluated using DPPH radical scavenging method. Results: Phytochemical analysis of the ethanolic extract of C. gynandra revealed the presence of alkaloids, phenolics, saponins, steroids, flavonoids, cardiac glycosides, and tannins. FTIR spectrum showed intense bands at 3679.18, 3616.63, 3317.34, 2943.67, 1634.01, 1360.20, 1036.71, and 778.04 cm−1 corresponding to N-H2, O-H stretch, aliphatic C-H stretch, C=O, C-H benzene, C-O stretch, and C-Cl. The total phenolic content was found to be 8.39 ± 0.0952 mg gallic acid equivalent/g and 66.76 ± 0.0333 mg rutin equivalent/g. The DPPH radical scavenging activity of ethanolic extract was showed more scavenging activity compared to ethyl acetate and n-hexane fractions. Conclusion: The present research work creates a platform to screen many bioactive chemical constituents present in C. gynandra to treat various diseases.

Extraction of Phenolic Compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using Reflux method and Screening of Antioxidant Activity in vitro

2021 ◽  
pp. 161-167
Author(s):  
Habchi A. ◽  
Dekmouche M. ◽  
Hamia C. ◽  
Saidi M. ◽  
Yousfi M. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Methanol Extract ◽  
Phoenix Dactylifera ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phoenix Dactylifera L ◽  
Phenolic And Flavonoid

This study was designed to evaluate the antioxidant activity of phenolic compounds for six cultivars of date from In Saleh region (Phoenix dactylifera L.), which were extracted by reflux with solvents, 80% methanol and 70% aceton after the phenolic and flavonoid content were estimated. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were measured using Folin–Ciocalteau and aluminum chloride colorimetric methods, respectively. Antioxidant activity acetone/H2O and methanol/H2O extracts using ABTS, DPPH, FRAP, TAC and CUPRAC assays. The results showed that methanolic solvent reflux gives better yields. The highest level of phenolic compounds for all dates varieties was found in the 80% methanol extract. This level ranged from 8.04 ± 0.14 to 46.81 ± 1.22 mg GAE/100 g fresh weight and flavonoid content was observed relatively low for both two solvents. However, extraction into 70% acetone gave the highest antioxidant activity with all assay. These results showed that dates had strongly scavenging activity on DPPH reaching 3.12 µg/mL in methanol/H2O from Cul1 (6.9 µg/mL with ascorbic acid), also on ABTS reaching 6.51 µg/mL in acetone/H2O from Cul2 (104.98 µg/mL with ascorbic acid).

scholarly journals Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn

2019 ◽  
Vol 40 ◽  
pp. 30-35
Author(s):  
Ishwor Pathak ◽  
Muna Niraula
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Ocimum Sanctum ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid

Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.

scholarly journals ISOLATION OF QUERCETIN FROM FLOWER PETALS, ESTIMATION OF TOTAL PHENOLIC, TOTAL FLAVONOID AND ANTIOXIDANT ACTIVITY OF THE DIFFERENT PARTS OF RHODODENDRON ARBOREUM SMITH

2015 ◽  
Vol 12 (12) ◽  
pp. 34-40 ◽  
Author(s):  
Laxman Bhandari ◽  
Meena Rajbhandari
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Acetone Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Different Parts

The flowers of Rhododendron arboreum Smith is a source of polyphenolic compounds. A flavonol, quercetin, was isolated from ethyl acetate fraction of the methanol extract of flower petals of R. arboreum by repeated Sephadex LH-20 column chromatography. Isolated quercetin was characterized by comparing melting point, Rf values, UV and IR spectra with authentic quercetin. The isolated quercetin was used as a standard for the estimation of total flavonoids.  Total phenolic and total flavonoid content in different parts of R. arboreum was carried out spectrophotometrically using Folin-Ciocalteu reagent and Aluminium chloride reagent respectively. Gallic acid and quercetin were used as standard for the construction of calibration curve of phenolic and flavonoid respectively. The results showed that the highest total phenolic content was detected in the 70% acetone extract of the flowers (600 mg GAE/g extract) and petals (600 mg GAE/g extract) and the lowest amount was detected in methanol extract of stem (188 mg GAE/g extract). Similarly, the highest total flavonoid was detected in the 70% acetone extract of the twigs (170 mg QE/g extract) and the lowest amount was detected in the methanol extract of stem (45 mg QE/g extract).  The antioxidant activity of the methanol extracts obtained from different parts of R. arboreum was determined by 2, 2- diphenyl-1-picryl hydrazyl (DPPH) assay and the radical scavenging activity (IC50) was calculated. The highest free radical scavenging effect was observed in leaves with IC50=8.34 ?g/ml and lowest was observed in stem with IC50=67.83 ?g/ml. The IC50 values, total phenolic and total flavonoid content (correlation coefficient R2= 0.923 for phenolic, R2= 0.965 for flavonoid) were correlated which showed strong correlation indicating that the major components responsible for antioxidant activity is phenolics. The highest the phenolic content, the lowest the IC50 value observed. The result indicated that R. arboreum is a rich source of high value polyphenols as natural antioxidant to use in preventive medicine as well as in food and pharmaceutical industry.Scientific World, Vol. 12, No. 12, September 2014, page 34-40         

scholarly journals ANTIOXIDANT ACTIVITY OF HALOPHILA OVALIS AND HALOPHILA BECCARII (HYDROCHARITACEAE): TWO IMPORTANT SEAGRASS SPECIES OF CHILIKA LAGOON, INDIA

2019 ◽  
pp. 136-140 ◽  
Author(s):  
SUBRAT KUMAR KAR ◽  
SUPRAVA SAHOO ◽  
BASUDEBA KAR ◽  
SOUMENDRA KUMAR NAIK ◽  
PRATAP CHANDRA PANDA
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Chilika Lagoon ◽  
Flavonoid Content ◽  
Halophila Ovalis ◽  
Ic50 Values

Objective: The present study was undertaken to evaluate the total phenolic and flavonoid content and the antioxidant property of two important seagrass species namely, Halophila ovalis and Halophila beccarii occurring in Chilika lagoon, Odisha, India. Methods: Total Phenolic Content (TPC) of the extracts of Halophila species was determined by Folin-Ciocalteu method with little modifications and the total flavonoid content (TFC) was measured by aluminum chloride colorimetric assay. The antioxidant activity of different extracts was investigated by DPPH and ABTS radical scavenging activity. IC50 values were calculated for the DPPH and ABTS methods. Result: The study revealed that the methanol extract of H. ovalis has greater antioxidant activity than H. beccarii. Methanol extract of both the species (H. ovalis and H. beccarii) was found to possess high phenolic content at value of 70.25 mg GAE/g of extract and 48.53 mg GAE/g of extract respectively. Similarly flavonoid contents was found highest in methanol extract for both H. ovalis (76.82 mg quercetin equivalent/ g of extract) and H. beccarii (64.28 mg quercetin equivalent/ g of extract). The antioxidant activity of different extracts of these two species were evaluated using DPPH and ABTS radical assay. The methanol extract of both H. ovalis and H. beccarii showed high radical scavenging activity with IC50 values of 37.77 μg/ml and 52.25 μg/ml for DPPH and 25.62 μg/ml and 45.45 μg/ml for ABTS respectively. Conclusion: The study revealed the potential of the Halophila species as natural sources of antioxidants having considerable commercial importance.

Comparative Phytochemicals and Antioxidant activity of various Solvent extracts of Zanthoxylum armatum leaves from different Geographical regions of Himachal Pradesh and their correlation analysis

2021 ◽  
pp. 2270-2276
Author(s):  
Manjula Gautam ◽  
Vikas Kumar ◽  
Sharma Sharma ◽  
Anuradha Sourirajan ◽  
Kamal Dev
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Chloroform Extract ◽  
Himachal Pradesh ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Zanthoxylum Armatum ◽  
Geographical Regions

Zanthoxylum armatum (family-Rutaceae) is a medicinally important and endangered plant species from Himalayan region. The objective of this study was to analyze the effect of different solvents on total phenolic content, total flavonoid content and antioxidant activity of Z. armatum leaves collected from different geographical regions of Himachal Pradesh. Extract of various solvents (chloroform, methanol, n-butanol and water) were prepared using cold maceration method. Total phenolic and flavonoid content of different extracts was quantified by spectrophotometric method. DPPH and FRAP assays were used to evaluate the antioxidant activity of different extracts of Z. armatum leaves. Among all the solvent extracts from different regions, methanol extract (9.26±1.13mg g-1 GAE) of leaves samples collected from Sirmour showed higher total phenolic content; while chloroform extract of Solan (10.7±0.29mg g-1 RE) showed higher flavonoid content. Chloroform extract of Sirmour (9.61±4.02µg ml-1) showed comparatively higher % DPPH radical scavenging activity; whereas, methanol extract of Shimla (55.92±2.0µM Fe (II) equivalents) showed higher FRAP activity among all solvents extract from all geographical regions. The present study also showed correlation of solvent with various parameters such as phenolic content, flavonoid content, DPPH and FRAP assay. These parameters also vary with geographical locations. The results from the present study validate the importance of leaves of Z. armatum as source of natural antioxidants.

scholarly journals Antioxidant activity, Total Phenolic and Flavonoid Contents and Cytotoxic activity of Euphorbia aegyptiaca

2020 ◽  
Vol 10 (2) ◽  
pp. 37-41
Author(s):  
Osman Adam Osman Adam ◽  
Ragaa Satti Mohmmed Abadi ◽  
Saad Mohamed Hussein Ayoub
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Soxhlet Apparatus ◽  
Promising Source ◽  
Phenolic And Flavonoid

Euphorbia aegyptiaca is a herbaceous plant traditionally used in Sudan for treatment of various diseases, and the study of this plant is still limited. The aim of the present study was to screen the phytochemicals and to assess the Antioxidant activity, total phenolic, flavonoid contents and cytotoxic activity of Euphorbia aegyptiaca. The plant material was extracted successively by Soxhlet apparatus using n-hexane, chloroform and methanol. The chemical constituents of the extracts were carried out using the standard procedures. The Folin- Ciocalteu and Aluminium chloride method was employed to calculate the total phenolic and flavonoid content, respectively. The antioxidant activity, was assessed by measuring the scavenging activity of the DPPH (2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl) and Propyl Gallate as standard antioxidants. While cytotoxic activities were screened using brine shrimp. Phytochemical screening studies revealed that flavonoids, tannins, coumarins, saponins, sterols, terpenes, anthraquinones and alkaloids were the main phytochemicals present in extracts of E. aegyptiaca. The methanol extract showed the highest level of total phenolic contents (173.49±2.427 mg GAE/g) and flavonoid content (239.53±7.90 mg QE/g), and the highest antioxidant activity 89% with least (IC50 0.0449µg/ml), and the no toxicity against brine shrimp (LD50 3423.156). Furthermore, no toxicity in all extracts was observed. The present study is the first evaluation regarding the characterization of E. aegyptiaca and its safety, and the results demonstrate its antioxidant potential and suggest its safe therapeutic use. The results suggest that methanol extract is a rich source of phytochemicals and exhibits highest amount of and total phenolic, flavonoid content and significant antioxidant activity and it has no cytotoxic activity. E. aegyptiaca plant can be regarded as a promising Source of naturally occurring potential antioxidants. Keywords: Euphorbia aegyptiaca, Sudan, Antioxidant, Total phenolic, total flavonoid, Cytotoxicity.

Uşak İli’nden Toplanan Taxus Baccata (L) Türünün Metanol Ekstresinin Yaprak, Dal ve Meyvesinde Biyolojik Aktivitenin Belirlenmesi

2019 ◽  
Vol 7 (10) ◽  
pp. 1533
Author(s):  
Yasemin Karafakıoglu
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Maximum Amount ◽  
Chemical Components ◽  
Taxus Baccata ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Future Studies ◽  
Different Parts

Taxus species in different parts of the world prevent the formation of hepatoprotective, anticoagulant, antiulcerogenic, anti-coagulant, antifungal and tumour cells. In this study, biological activity studies were performed on the leaves, branches and fruits of the methanol extract of Taxus baccata L. species collected from Sivaslı district of Uşak province. The methanol extract of the T.baccata species, the total amount of phenolic content of the sample was found to be 14.76 mg GAEQ/1g dry sample in the highest leaves. In T.baccata methanol extract, the maximum amount of flavonoid content was 0.468±mg QE/g dry sample. Antioxidant activity value as DPPH radical; % inhibition value was found as 93.21%. Based on the results; It can be concluded that the leaves of T.baccata have higher antioxidant activity than fruits and branches. Future studies need to explore the chemical components contained in the T.baccata species to determine and investigate in further detail.

scholarly journals Antifungal and Antioxidant Potential of Methanolic Extracts from Acorus calamus L., Chlorella vulgaris Beijerinck, Lemna minuta Kunth and Scenedesmus dimorphus (Turpin) Kützing

2021 ◽  
Vol 11 (11) ◽  
pp. 4745
Author(s):  
Toncho Dinev ◽  
Milena Tzanova ◽  
Katya Velichkova ◽  
Diyana Dermendzhieva ◽  
Georgi Beev
Keyword(s):  
Antioxidant Activity ◽  
Chlorella Vulgaris ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Acorus Calamus ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Methanolic Extracts ◽  
Scenedesmus Dimorphus

Plant extracts are an important alternative to antibiotics, which are ever more restricted because of their developing microbial resistance and some adverse effects that have been observed following frequent application. The aim of the present study was to determine the antifungal and antioxidant activity of the methanolic extracts of Acorus calamus, Chlorella vulgaris, Lemna minuta and Scenedesmus dimorphus. The antifungal activity of the extracts against strains of Aspergillus flavus, Aspergillus parasiticus, Aspergillus ochraceus, Aspergillus niger, Aspergillus carbonarius, Fusarium graminearum, Fusarium oxysporum, Penicillium chrysogenum and Alternaria alternata was evaluated via the agar well diffusion method. The antioxidant activity of the extracts was measured through the determination of three parameters—total phenolic content, total flavonoid content and radical scavenging potential (determined through UV/Vis analysis). A. calamus extracts had the highest antimicrobial activity against eight fungal strains, followed by the C. vulgaris, L. minuta and S. dimorphus extracts, which were inhibitory against two to three strains. Among the extracts from the species studied, the extract from S. dimorphus showed the highest antioxidant potential, as determined via the DPPH (1,1’-diphenyl-2-picrylhydrazil-radical) method. This correlated to its high total phenolic and flavonoid content. From A. calamus and L. minuta, methanolic extracts were obtained that exhibited similar values of the aforementioned parameters, followed by C. vulgaris extracts, which showed the lowest antioxidant activity. Based on the Pearson correlation coefficients, the impacts of the total phenolic content and the total flavonoid content on radical scavenging capacity are similar, and flavonoids were a significant part of the total phenolic compounds extracted from the plant materials studied.

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