Antioxidant activity, Total Phenolic and Flavonoid Contents and Cytotoxic activity of Euphorbia aegyptiaca

Euphorbia aegyptiaca is a herbaceous plant traditionally used in Sudan for treatment of various diseases, and the study of this plant is still limited. The aim of the present study was to screen the phytochemicals and to assess the Antioxidant activity, total phenolic, flavonoid contents and cytotoxic activity of Euphorbia aegyptiaca. The plant material was extracted successively by Soxhlet apparatus using n-hexane, chloroform and methanol. The chemical constituents of the extracts were carried out using the standard procedures. The Folin- Ciocalteu and Aluminium chloride method was employed to calculate the total phenolic and flavonoid content, respectively. The antioxidant activity, was assessed by measuring the scavenging activity of the DPPH (2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl) and Propyl Gallate as standard antioxidants. While cytotoxic activities were screened using brine shrimp. Phytochemical screening studies revealed that flavonoids, tannins, coumarins, saponins, sterols, terpenes, anthraquinones and alkaloids were the main phytochemicals present in extracts of E. aegyptiaca. The methanol extract showed the highest level of total phenolic contents (173.49±2.427 mg GAE/g) and flavonoid content (239.53±7.90 mg QE/g), and the highest antioxidant activity 89% with least (IC50 0.0449µg/ml), and the no toxicity against brine shrimp (LD50 3423.156). Furthermore, no toxicity in all extracts was observed. The present study is the first evaluation regarding the characterization of E. aegyptiaca and its safety, and the results demonstrate its antioxidant potential and suggest its safe therapeutic use. The results suggest that methanol extract is a rich source of phytochemicals and exhibits highest amount of and total phenolic, flavonoid content and significant antioxidant activity and it has no cytotoxic activity. E. aegyptiaca plant can be regarded as a promising Source of naturally occurring potential antioxidants. Keywords: Euphorbia aegyptiaca, Sudan, Antioxidant, Total phenolic, total flavonoid, Cytotoxicity.

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Extraction of Phenolic Compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using Reflux method and Screening of Antioxidant Activity in vitro

Asian Journal of Research in Chemistry ◽

10.52711/0974-4150.2021.00030 ◽

2021 ◽

pp. 161-167

Author(s):

Habchi A. ◽

Dekmouche M. ◽

Hamia C. ◽

Saidi M. ◽

Yousfi M. ◽

...

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Phenolic Compounds ◽

Methanol Extract ◽

Phoenix Dactylifera ◽

Total Phenolic ◽

Flavonoid Content ◽

Phoenix Dactylifera L ◽

Phenolic And Flavonoid

This study was designed to evaluate the antioxidant activity of phenolic compounds for six cultivars of date from In Saleh region (Phoenix dactylifera L.), which were extracted by reflux with solvents, 80% methanol and 70% aceton after the phenolic and flavonoid content were estimated. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were measured using Folin–Ciocalteau and aluminum chloride colorimetric methods, respectively. Antioxidant activity acetone/H2O and methanol/H2O extracts using ABTS, DPPH, FRAP, TAC and CUPRAC assays. The results showed that methanolic solvent reflux gives better yields. The highest level of phenolic compounds for all dates varieties was found in the 80% methanol extract. This level ranged from 8.04 ± 0.14 to 46.81 ± 1.22 mg GAE/100 g fresh weight and flavonoid content was observed relatively low for both two solvents. However, extraction into 70% acetone gave the highest antioxidant activity with all assay. These results showed that dates had strongly scavenging activity on DPPH reaching 3.12 µg/mL in methanol/H2O from Cul1 (6.9 µg/mL with ascorbic acid), also on ABTS reaching 6.51 µg/mL in acetone/H2O from Cul2 (104.98 µg/mL with ascorbic acid).

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Influence of total phenolic content and total flavonoid content on the DPPH radical scavenging activity of Costus speciosus (Koen Ex. Retz.) Sm.

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.4(5).p261-266 ◽

2015 ◽

Vol 4 (5) ◽

pp. 261-266

Author(s):

M. Borkataky ◽

B. B. Kakoti ◽

L. R. Saikia

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Flavonoid ◽

Aluminium Chloride ◽

Total Phenolic ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Costus Speciosus ◽

Phenolic And Flavonoid

The focus of the present study was to determine the association of the anti-oxidant activity of Costus speciosus (Koen Ex. Retz.) Sm. with the total phe-nolic content and the total flavonoid content. The antioxidant activity of the extracts was quantified by diphenylpicrylhydrazyl (DPPH) assay. The total phenolic content was determined by the Folin-Ciocalteau method and the total flavonoid content was determined by the aluminium chloride method. Among the four extracts of the plant, the ethanol extract exhibited the high-est antioxidant activity and also the highest total phenolic and flavonoid con-tents. A strong correlation was observed between the antioxidant activity and the total phenolic and flavonoid contents. A statistical model was de-rived to explain this dependence and a non-linear association was observed between the antioxidant activity and the total flavonoid content.

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Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn

Journal of Nepal Chemical Society ◽

10.3126/jncs.v40i0.27275 ◽

2019 ◽

Vol 40 ◽

pp. 30-35

Author(s):

Ishwor Pathak ◽

Muna Niraula

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Colorimetric Method ◽

Radical Scavenging ◽

Hexane Extract ◽

Ocimum Sanctum ◽

Total Phenolic ◽

Flavonoid Content ◽

Ic50 Value ◽

Phenolic And Flavonoid

Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.

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Antioxidant activity of Clematis brachiata Thunb. leaf

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v53i3.38264 ◽

2018 ◽

Vol 53 (3) ◽

pp. 185-190 ◽

Cited By ~ 1

Author(s):

M Mostafa ◽

S Ahmed ◽

AJ Afolayan

Keyword(s):

Antioxidant Activity ◽

Dry Matter ◽

Methanol Extract ◽

Total Phenolics ◽

Colorimetric Method ◽

Aluminium Chloride ◽

Total Phenolic ◽

Good Source ◽

Diammonium Salt ◽

Phenolic And Flavonoid

The antioxidant activity, total phenolic and flavonoid contents of different extracts of the Clematis brachiata Thunb leaves were determined. The antioxidant activity was evaluated using spectroscopic methods against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2′-azinobis [3-ethylbenzothiazoline-6-sulphonic acid] diammonium salt radical cation (ABTS). Folin Ciocalteu method was used to determine the total phenolics and Aluminium Chloride Colorimetric method was used to determine the flavonoids contents in these extracts. The results showed that the methanol extract of the leaf exhibited the highest antioxidant activity with the value of 180.45 ±2.4 μg mL-1 in DPPH and 60 ±0.80 μg mL-1 in ABTS assay among the extracts. The methanol extract contains more phenolic compounds (178±2.20 mg/g as galic acid equivalent per g dry matter) and the acetone extract contains more flavonoids (135.11±1.20 mg/g as quercitin equivalent per g dry matter) among the extracts. This study provides the evidence that the leaves of the Clematis brachiata Thunb could be a good source of natural antioxidant.Bangladesh J. Sci. Ind. Res.53(3), 185-192, 2018

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Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v18i7.20 ◽

2021 ◽

Vol 18 (7) ◽

pp. 1499-1506

Author(s):

Nacer Amraoui ◽

Nozha Mayouf ◽

Noureddine Charef ◽

Abderrahmane Baghiani ◽

Lekhmici Arrar

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Folk Medicine ◽

Dry Weight ◽

Aluminium Chloride ◽

Total Phenolic ◽

Paw Edema ◽

Adjuvant Induced Arthritis ◽

Anti Inflammatory ◽

Phenolic And Flavonoid

Purpose: To evaluate the antioxidant, anti-inflammatory and anti‐arthritic effects of the methanol extract of Tamus communis roots. Methods: The total phenolic and flavonoid contents were determined using Folin–Ciocalteu’s reagent and aluminium chloride assays, respectively while the antioxidant capacity was determined using DPPH and β-carotene/linoleate tests. For anti-inflammatory activity, carrageenan-induced paw edema in rat was used. Formaldehyde-induced paw edema and adjuvant-induced arthritis in rats were used to evaluate the anti-arthritic effect of the extract. Results: The total phenolic and flavonoid contents of T. communis showed 55.2 ± 0.036 mg GAE/g dry weight and 1.191 ± 0.002 mg QEq/g dry weight, respectively while the antioxidant activity (DPPH) showed a half-maximal inhibitory concentration (IC50 of 0.128 ± 0.011 mg/mL. Using βcarotene/linoleate assay, the extract showed 88.13 ± 4.03 % inhibition. In carrageenan-induced paw edema in rat, all doses of T. communis did not show inhibition of edema. In contrast, formaldehyde induced paw edema decreased at the dose of 150 mg/kg days 2 and 4. However, doses of 300 and 600 mg/kg did not show any activity. In adjuvant-induced arthritis, all used doses caused no reduction in arthritic scores and in paw thickness except a significant decrease with the dose 150 mg/kg at the last day. Conclusion: T. communis extract exhibits high antioxidant activity related to the phenolic compound contents. However, the results of the anti-inflammatory and anti‐arthritic studies did not support its use in folk medicine in the treatment of rheumatic ailment except on low doses

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Molecular and phytochemical characterization of F1 Streptocarpus hybrids and antioxidant potential of their flower extracts

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha48311959 ◽

2020 ◽

Vol 48 (3) ◽

pp. 1341-1356

Author(s):

Monica HȂRŢA ◽

Doru PAMFIL ◽

Orsolya BORSAI ◽

Rodica POP ◽

Doina CLAPA ◽

...

Keyword(s):

Antioxidant Activity ◽

Genetic Relationships ◽

Flower Colour ◽

Chemical Compounds ◽

Aluminium Chloride ◽

Total Phenolic ◽

Flavonoid Content ◽

Polyphenolic Content ◽

Pharmaceutical Industries ◽

Phenolic And Flavonoid

Streptocarpus x hybridus is a very popular houseplant with colourful flowers arousing thus the interest of plant breeders. Its potential value is even higher since different chemical compounds could be identified and used for food and pharmaceutical industries. Therefore, the objectives of this study were: to determine genetic relationships between twelve F1 Streptocarpus hybrids and their genitors; to determine the total phenolic and flavonoid content; to evaluate the antioxidant activity of the analysed individuals. Although 12 out of the 24 RAPD primers detected polymorphism among the individuals, the UPGMA dendrogram did not show a very clear grouping pattern for flower colour. In contrast, the phytochemical dendrogram shed more light on the dissimilarities among Streptocarpus flowers in terms of their polyphenolic content. The total phenolic and flavonoid content of the flower extracts determined by the Folin-Ciocalteau and aluminium chloride colorimetric methods ranged from 680.02 to 2360.57 mg of GAE/100 g FW and from 12.52 to 78.36 mg QE/100 g FW. The radical scavenging activity against ABTS ranged from 27.17 to 130.35 µM Trolox/g FW. Due to its ornamental value, H12 was screened by HPLC-PDA-ESI-MS to identify and quantify the chemical compounds involved in flower colour. Among the six compounds that were identified and quantified (i.e. cyanidin-3-O-galactoside, cyanidin-3-O-xyloside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, peonidin-3-O-glucoside and apigenin-6-C-glucoside), isovitexin reached the highest concentration 4183 µg rutin/100 g FW. These preliminary results, showing high total polyphenolic content and ABTS antioxidant activity, suggest that Streptocarpus flowers could be a valuable source of antioxidants.

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Antioxidant activity and total phenolic and flavonoid contents of Dendrobium amoenum Wall. ex Lindl.

Botanica Orientalis Journal of Plant Science ◽

10.3126/botor.v9i0.21010 ◽

2015 ◽

Vol 9 ◽

pp. 20-26 ◽

Cited By ~ 5

Author(s):

Mukti Ram Poudel ◽

Mukesh Babu Chand ◽

Namrata Karki ◽

Bijaya Pant

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Phenolic Content ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Biologically Active ◽

Aluminium Chloride ◽

Total Phenolic ◽

Flavonoid Content ◽

Phenolic And Flavonoid

Dendrobium amoenum has biologically active phytoconstituents valued for traditional Chinese or folk medicine as tonic. Total phenolic and flavonoid contents of its stem extract was estimated by using Folin-Ciocalteu’s reagent and Aluminium chloride methods respectively. Antioxidant activity was determined by DPPH free radical scavenging method. Total phenolic content found in acetone (134.34 μg GAE/mg extract) and chloroform (101.55 μg GAE/mg extract) extract was significantly higher than other solvent extracts. Similarly, these two extracts had significantly high flavonoid content (acetone: 115.73 μg QE/mg extract, and chloroform: 84.16 μg QE/mg extract). Presence of high phenolic and flavonoid contents in these extracts showed the highest antioxidant activity. Highest antioxidant activity of these extracts was determined by their lowest IC50 value (acetone: 53.19 μg/ml and chloroform: 36.48 μg/ml). Significant negative relationship was found between phenolic content and IC50 (R2 = 0.209, p < 0.01) and flavonoid content and IC50 (R2 = 0.389, p < 0.01), which indicates high antioxidant activity due to high phenolic and flavonoid contents. This result revealed that D. amoenum act as an antioxidant agent due to its free radical scavenging activity which plays a crucial role in the development of new chemotherapeutic agents.

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NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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Spectrophotometric determination of sun protective factor (SPF) and antioxidant potential of different solvent extracts of graptophyllum pictum

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10ispl1.1722 ◽

2019 ◽

Vol 10 (SPL1) ◽

Author(s):

Noor Diyana Ibrahim ◽

Wan Nur Farihah Che Mustafa ◽

Mahendran Sekar ◽

Khor Poh Yen ◽

Seow Lay Jing

Keyword(s):

Total Phenolic Content ◽

Methanol Extract ◽

Phenolic Content ◽

Total Flavonoid ◽

Antioxidant Potential ◽

Aluminium Chloride ◽

Total Phenolic ◽

Uv Spectrophotometry ◽

Total Flavonoid Content ◽

Flavonoid Content

Natural substances extracted from plants have been gaining attraction as protective agents due to their safety and responsible for multiple biological effects on skins. The present study was to determine the total phenolic and flavonoid contents, Sun Protection Factor (SPF), and antioxidant potential of different solvent extracts of Graptophyllum pictum leaves. The dried powdered leaves were extracted by the cold maceration method, using three different solvents, i.e., methanol, ethanol, and water. The extracted leaves was tested for antioxidant activity using of DPPH (1,1-diphenyl-2-picryl-hydrazyl). Total phenolic content was determined using the Follin-Ciocalteu and for total flavonoid content with used the aluminium chloride. The SPF of all extracts were analyzed by ultraviolet (UV) spectrophotometry. The total phenolic content is the highest in methanol extract (377.61±0.31 µg/ml), and total flavonoid content is the highest in ethanol extract (158.06 ± 0.18 µg/ml). For DPPH free radical scavenging activity, ethanolic extract showed the maximum percentage of inhibition, and for SPF, methanol extract had displayed the highest. The study recommends that methanol is a good solvent for the extraction of sun-protective constituents from G. pictum.

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Evaluation of Antioxidant Potential of Stem and Leaf Extracts of Himalayan Tinospora cordifolia Hook.f. & Thomson

The Open Bioactive Compounds Journal ◽

10.2174/1874847302109010002 ◽

2021 ◽

Vol 9 (1) ◽

pp. 2-8

Author(s):

Geetanjali Upadhyay ◽

Lalit M. Tewari ◽

Geeta Tewari ◽

Neha Chopra ◽

Naveen C. Pandey ◽

...

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Radical Scavenging ◽

Aluminium Chloride ◽

Tinospora Cordifolia ◽

Total Phenolic ◽

Flavonoid Content ◽

Leaf Extracts ◽

The Family ◽

Stem Extract

Background: Medicinal plants are considered a rich source of ingredients, which can be used in drug development and synthesis. Tinospora cordifolia (Wild.) Hook.f. & Thomson, commonly known as guduchi, heart-leaved moonseed and giloya is a herbaceous vine of the family Menispermaceae, has several beneficial properties including antioxidant activity. Aim: The present study was carried out to analyze the antioxidant activity of leaf and stem extracts of Tinospora cordifolia by using DPPH (1,1-Diphenyl-2-picrylhydrazyl) and ABTS (2,2´-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) free radical scavenging assays. Materials and Methods: Dried and powdered leaves and stem of T. cordifolia were extracted with methanol. Ascorbic acid was taken as standard. Total phenolic content was estimated by using Folin-ciocalteu's reagent while total flavonoid content by aluminium chloride reagent to find the correlation of polyphenols with antioxidant activity. ABTS assay of methanolic leaf and stem extracts showed the highest scavenging activity as compared to the DPPH assay. Results: Methanolic stem extract showed higher phenolic and flavonoid content along with antioxidant activity as compared to the methanolic leaf extract. Conclusion: The stem extract exhibited more antioxidant activity than the leaf extract with regards to the all parameters analyzed.

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