Influence of Navisporus floccosus (Higher Basidiomycetes) n-Hexane Extract on Arachidonate Metabolism in Vitro

2015 ◽  
Vol 17 (5) ◽  
pp. 463-469
Author(s):  
Vivian Leliebre-Lara ◽  
Eva-Maria Pferschy-Wenzig ◽  
Ute Widowitz ◽  
Sabine Ortmann ◽  
Clara Nogueiras Lima ◽  
...  
Keyword(s):  
Hexane Extract ◽  
Arachidonate Metabolism

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals In vitro antioxidant and antidiarrheal activities of aqueous and n-hexane extracts of Cucurbita maxima seed in castor oil-induced diarrheal rats

2022 ◽  
Author(s):  
Habibu Tijjani ◽  
Adamu Matinja ◽  
Marwanatu Yahya ◽  
Emmanuel Aondofa ◽  
Akibu Sani
Keyword(s):  
Vitamin C ◽  
Aqueous Extract ◽  
Castor Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Seed Extract ◽  
Hexane Extract ◽  
Cucurbita Maxima ◽  
Seed Extracts

Diarrhea is a common health complaint occurring with mild, temporary conditions to a potentially life-threatening condition. <i>Cucurbita maxima</i> (Cucurbitaceae) seed is reportedly used traditionally for the treatment of diarrheal and thus this study aimed to evaluate the in vitro antioxidants, total tannins, phenolics contents, and antidiarrheal potentials of <i>n</i>-hexane and aqueous <i>C. maxima</i> seed extracts in castor oil induced diarrheal rats. The <i>n</i>-hexane extract mainly contains oils while the aqueous extract was thick, brown solid. The aqueous and <i>n</i>-hexane seed extract of <i>C. maxima</i> expressed significant 2,2, diphenyl-1-picrythdrazyl (DPPH) scavenging activities at 6.25 – 1000 mg/mL with IC<sub>50</sub> values of 104.01 mg/mL, 29.27 mg/mL and 26.78 mg/mL for <i>n</i>-hexane, aqueous and vitamin C respectively. The hydroxyl radical scavenging activities of the <i>n</i>-hexane and aqueous seed extract of <i>C. maxima</i> were significantly lower at higher concentrations compared with that of vitamin C. Furthermore, the total antioxidant capacities of <i>n</i>-hexane (22.08mg/mL) and aqueous seed extract (11.03 mg/mL) of <i>C. maxima</i> were found to be higher than that of vitamin C (134.46 mg/mL). The aqueous extracts (658.33±380.08 mg QE/g) total tannins were not significantly different from the <i>n</i>-hexane extract (468.33±102.55 mg QE/g) while the <i>n</i>-hexane extract contains significantly higher total phenolics (2.93±1.25 mg GAE/g) compared with the aqueous extract (0.19±0.04 mg GAE/g). Percentage inhibition of stooling was found to be 57±22%, 41±16%, and 46±11% for loperamide, <i>n</i>-hexane, and aqueous respectively. The study concludes that aqueous and n-hexane seed extracts of <i>C. maxima</i> (pumpkin) possess in vitro antioxidant activities and antidiarrheal properties.

scholarly journals Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

2020 ◽  
Vol 151 ◽  
pp. 01029
Author(s):  
Nuzul Asmilia ◽  
Mahdi Abrar ◽  
Yudha Fahrimal ◽  
Amalia Sutriana ◽  
Yobeswi Husna
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Hexane Extract ◽  
Clear Zone ◽  
Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

scholarly journals In vitro Antiprotozoal Activity of Extracts of five Turkish Lamiaceae Species

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Hasan Kirmizibekmez ◽  
Irem Atay ◽  
Marcel Kaiser ◽  
Erdem Yesilada ◽  
Deniz Tasdemir
Keyword(s):  
Trypanosoma Brucei ◽  
Leishmania Donovani ◽  
Hexane Extract ◽  
Significant Activity ◽  
Trypanosoma Brucei Rhodesiense ◽  
L6 Cells ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Organic Extracts

The in vitro antiprotozoal activities of crude methanolic extracts from the aerial parts of five Lamiaceae plants ( Salvia tomentosa, S. sclarea, S. dichroantha, Nepeta nuda subsp. nuda and Marrubium astracanicum subsp. macrodon) were evaluated against four parasitic protozoa, i.e. Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani and Plasmodium falciparum. The cytotoxic potentials of the extracts on L6 cells were also evaluated. Melarsoprol, benznidazole, miltefosine, chloroquine and podophyllotoxin were used as reference drugs. All crude MeOH extracts showed antiprotozoal potential against at least three parasites, so they were dispersed in water and partitioned against n-hexane and chloroform to yield three subextracts that were screened in the same test systems. The n-hexane extract of N. nuda was the most active against T. brucei rhodesiense while the CHCl3 extracts of S. tomentosa and S. dichroantha showed significant activity against L. donovani. All organic extracts displayed in vitro antimalarial and moderate trypanocidal activities against T. cruzi with the n-hexane extract of S. sclarea being the most active against the latter. The extracts displayed low or no cytotoxicity towards mammalian L6 cells.

Arachidonate metabolism increases as rat alveolar type II cells differentiate in vitro

1990 ◽  
Vol 259 (2) ◽  
pp. L73-L80 ◽  
Author(s):  
R. J. Lipchik ◽  
J. B. Chauncey ◽  
R. Paine ◽  
R. H. Simon ◽  
M. Peters-Golden
Keyword(s):  
Primary Cultures ◽  
Alveolar Type ◽  
Ionophore A23187 ◽  
Type Ii Cells ◽  
Type Ii ◽  
In Vitro Differentiation ◽  
Eicosanoid Synthesis ◽  
Arachidonate Metabolism ◽  
Cells Cultured

Rat type II alveolar epithelial cells are known to undergo morphological and functional changes when maintained in culture for several days. Having previously demonstrated that these cells can deacylate free arachidonic acid (AA) and metabolize it to products of the cyclooxygenase pathway, the present study was undertaken to determine whether in vitro differentiation was accompanied by alterations in the availability and metabolism of AA. We assessed the constitutive and ionophore A23187-induced deacylation and metabolism of endogenous AA, as well as the metabolism of exogenously supplied AA, in primary cultures of rat type II cells at days 2, 4, and 7 after isolation. Levels of free endogenous AA were increased at day 4, whereas eicosanoid synthesis, predominantly prostaglandin E2 and prostacyclin, increased markedly only at day 7. A similar time course of augmentation of prostanoid release was seen in response to exogenous AA. Type II cells cultured on fibronectin, intended to hasten cell flattening and spreading, demonstrated accelerated increases in available free AA in response to A23187; cells cultured on basement membrane derived from Engelbreth-Holm-Swarm mouse sarcoma, known to maintain the type II phenotype, exhibited diminished levels of available free AA. From these findings, we conclude that alterations in arachidonate metabolism are linked to alterations in cellular phenotype. The potentiation of eicosanoid synthesis accompanying in vitro differentiation suggests a possible role for the alveolar epithelium in the modulation of inflammation and fibrosis in the distal lung.

scholarly journals Isolation of stigmasterol from hexane extract of leaves of Pisonia grandis R.Br, in vitro anti-diabetic and its molecular docking studies

2020 ◽  
Vol 11 (2) ◽  
pp. 2117-2122
Author(s):  
Abhijit Mitra ◽  
Mohankumar Ramasamy ◽  
Valentina Parthiban ◽  
Thottempudi Ravi Teja ◽  
Srikalyani Vemuri ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Docking Studies ◽  
Hexane Extract ◽  
Medicinal Uses ◽  
Isolation And Purification ◽  
Molecular Docking Studies ◽  
Ic50 Value ◽  
Isolated Compound ◽  
Pisonia Grandis

Pisonia grandis R.Br belonging to the family Nyctaginaceae is a widely distributed evergreen tree in India known for its medicinal uses. The study was aimed to investigate the anti-diabetic property in the leaves of Pisonia grandis R.Br. The isolation and purification were performed by the conventional column chromatography and the resultant yield was found to be a white crystalline powder, which was further subjected for characterization through IR, 1H NMR, 13C NMR and Mass spectroscopy. From the characterization data, the isolated compound was identified as stigmasterol, it was first time isolated from the hexane extract of the leaves. The α-amylase inhibitory activity of stigmasterol from the hexane extract of the leaves of Pisonia grandis R.Br showed high potent activity with an IC50 value of 46μg/ml. The anti-diabetic activity of the compound against α-amylase and four other diabetic enzymes- α-glucosidase acid phosphatase, endo-β-N acetaglucosaminidase and β-glucuronidase were further investigated by molecular docking studies and proved that stigmasterol can be a potential anti-diabetic agent.

scholarly journals PHOTOPROTECTIVE, CYTOTOXIC AND PHOTOMODIFYING ACTIVITY OF LICHEN BIOMASS NON-POLAR FRACTIONS AGAINST HUMAN KERATINOCYTE (HaCAT)

2021 ◽  
Vol 2 ◽  
pp. 29-35
Author(s):  
Volga M. Khramchankova ◽  
◽  
Matsvei V. Matveyenkau ◽  
Keyword(s):  
Ultraviolet Radiation ◽  
Ultraviolet Irradiation ◽  
Human Keratinocytes ◽  
Hexane Extract ◽  
Hypogymnia Physodes ◽  
Evernia Prunastri ◽  
Human Keratinocyte ◽  
Xanthoria Parietina ◽  
Benzene Extract

In vitro, the photoprotective, cytotoxic and photomodifying properties of hexane and benzene extracts from the widely distributed in Belarus lichens Cladonia arbuscula, Evernia prunastri, Hypogymnia physodes, Ramalina pollinaria and Xanthoria parietina in the culture of human keratinocytes (HaCAT), are estimated. It was found that these extracts are not photoprotective, they have a photomodifying effect upon irradiation of keratinocyte cultures with ultraviolet light. Hexane extract of E. prunastri and benzene extracts of R. pollinaria and H. physodes at a concentration of 2.5 μg/ml are photoprotectors that weaken the effect of ultraviolet radiation by 1.6 ÷ 1.8 times. Extracts of C. arbuscula and X. parietina are photosensitizers – they increase the toxic effect of ultraviolet radiation by 10 or more times, regardless of the concentration of the extract in the nutrient medium. Hexane and benzene extracts of C. arbuscula and H. physodes lichens are cytotoxic to the HaCAT culture. With an increase in the dose of ultraviolet irradiation of keratinocytes from zero to lethal values, the benzene extract of R. pollinaria acted as a photoprotector at a concentration of 2.5 μg / ml, and as a photosensitizer at higher concentrations. The hexane and benzene extracts of E. prunastri and H. physodes exhibited sensitizing properties that increased with an increase in the concentration of lichen extracts. C. arbuscula hexane extract and X. parietina benzene extract were the most potent photosensitizers.

scholarly journals In vitro antioxidant anti inflammatory and cytotoxicity activities from Hexane extract of Bryonopsis laciniosa fruits.

2018 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Sanjeevkumar C.B ◽  
Ramesh L Londonkar ◽  
Umesh Madire Kattegouda
Keyword(s):  
Cell Line ◽  
Potential Application ◽  
Protein Denaturation ◽  
Cancer Cell Line ◽  
Hexane Extract ◽  
Inflammatory Activity ◽  
Cytotoxicity Activity ◽  
Anti Inflammatory ◽  
Mcf 7

<p><em>Bryonopsis laciniosa </em>also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India<em>. </em>As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of <em>B. laciniosa </em>fruits were used to evaluate <em>in</em> <em>vitro</em> anti inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities.<em> In vitro</em> anti inflammatory activity by inhibition of protein denaturation, antioxidant assays like DPPH, ABTS, H<sub>2</sub>O<sub>2</sub> and FRAP were used to measure the antioxidant capacity of the hexane extracts and cytotoxicity activity using MCF-7 breast cancer cell line. Hexane extract showed the effective antioxidant activity in all assays compared to ascorbic acid and BHT. The results for<em> In vitro</em> anti inflammatory activity of hexane extract and Dichlofenac drug were equivalent, hexane extract showed promising activity for inhibition of protein denaturation assay. The cytotoxicity activity from hexane extract was noticeable against MCF-7 cell line. The overall results show potential application of <em>Bryonopsis laciniosa </em>fruits suggesting their potential application as a health-promoting functional ingredient or natural preservative in foods.</p>

scholarly journals In vitro and in vivo investigation of polypharmacology of propolis extract as anticancer, antibacterial, anti-inflammatory, and chemical properties

2021 ◽  
Vol 19 (1) ◽  
pp. 864-874
Author(s):  
Nael Abutaha ◽  
Mohammed AL-Zharani ◽  
Amal Alotaibi ◽  
Mary Anne W. Cordero ◽  
Asmatanzeem Bepari ◽  
...  
Keyword(s):  
Chemical Properties ◽  
Acid Methyl Ester ◽  
Cost Effective ◽  
Skin Inflammation ◽  
Hexane Extract ◽  
Major Constituent ◽  
Propolis Extract ◽  
Anti Inflammatory

Abstract Numerous compounds derived from natural sources such as microbes, plants, and insects have proven to be safe, efficacious, and cost-effective therapeutics for human diseases. This study examined the bioactivities of propolis, a structural sealant and antibacterial/antifungal agent produced by honey bees. Chinese propolis was extracted in methanol or hexane. Propolis significantly reduced the numbers of viable cancer cells when applied as a methanol extract (IC50 values in μg/mL for the indicated cell line: MDA-MB-231, 74.12; LoVo, 74.12; HepG2, 77.74; MCF7, 95.10; A549, 114.84) or a hexane extract (MDA-MB-231, 52.11; LoVo, 45.9; HepG2, 52.11; MCF7, 78.01; A549, 67.90). Hexane extract also induced apoptosis of HepG2 cells according to activated caspase-3/7 expression assays (17.6 ± 2.9% at 150 μg/mL and 89.2 ± 1.9% at 300 μg/mL vs 3.4 ± 0.4% in vehicle control), suppressed the growth of Candida albicans and multiple multidrug-resistant and nonresistant Gram-positive bacteria, and inhibited croton oil-induced skin inflammation when applied as topical treatment. GC-MS identified hexadecanoic acid methyl ester as a major constituent (33.6%). Propolis hexane extract has potential anticancer, antimicrobial, and anti-inflammatory activities.

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