QUANTITATIVE DETERMINATION OF IODOAMINO ACIDS IN THE THYROID GLAND OF THE PARROT-FISH (SCARUS GUACAMAIA)

1970 ◽  
Vol 46 (4) ◽  
pp. 417-423 ◽  
Author(s):  
A. J. MATTY ◽  
C. C. THORNBURN
Keyword(s):  
Body Weight ◽  
Quantitative Analysis ◽  
Thyroid Gland ◽  
Quantitative Determination ◽  
Sex Difference ◽  
Total Content ◽  
Parrot Fish

SUMMARY A quantitative analysis of the iodoamino acids in whole dried parrot-fish thyroid gland was carried out by determination of stable 127I. Mono- and di-iodotyrosine together made up about half of the total iodoamino acids and thyroxine about a third to a half. The tri-iodothyronine content was low, about 5%. The total content of the acids increased with body weight, and there was an indication of a sex difference.

scholarly journals Current Approaches to Valerian Tincture Standardisation in Terms of Assay

2019 ◽  
Vol 9 (4) ◽  
pp. 265-271
Author(s):  
N. P. Antonova ◽  
E. P. Shefer ◽  
A. M. Kalinin ◽  
N. E. Semenova ◽  
S. S. Prokhvatilova ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Determination ◽  
High Performance ◽  
Test Procedure ◽  
Total Content ◽  
Reference Standard ◽  
Active Ingredients ◽  
Test Procedures ◽  
Valerenic Acid

The quality control of the «Valerian rhizome and roots» herbal substance is carried out using high performance liquid chromatography (HPLC) according to the State Pharmacopoeia of the Russian Federation, XIV edition. The quantitative analysis of the active ingredients in valerian tincture is performed using a non-specific and non-selective spectrophotometric method. Therefore, it is important to introduce in Russia a more modern test procedure for quantitative determination of active ingredients in valerian tincture.The aim of the study was to develop a selective and sensitive HPLC procedure for quantitative determination of the total content of sesquiterpenic acids, expressed as valerenic acid, for the purpose of valerian tincture standardisation.Materials and methods: valerian tincture samples produced by seven Russian manufacturers were used as test samples, and valerenic acid was used as the reference standard. The quantitative analysis of the active ingredients was performed by two methods: spectrophotometry at 512 nm following the reaction of valerenic acid ethylester with hydroxyalamine and ferric chloride, and by HPLC using a Nucleosil C18 column, 125×4.6 mm, 5 µm particle size, in gradient elution mode, with detection at 220 nm.Results: the spectrophotometric technique was shown to be insufficiently specific. The authors of the study validated the chromatographic test procedure, established system suitability criteria, and compared the results obtained by the two test procedures. They also determined a tentative standard of the total content of sesquiterpenic acids, expressed as valerenic acid, obtained by HPLC.Conclusions: the HPLC assay developed for quantitative determination of active ingredients in valerian tincture is more specific as compared to the spectrophotometric technique, as the sum of the peaks of valerenic and acetoxyvalerenic acids and the results for the reference standard are taken into account during calculations. The new test procedure is in line with the cross-cutting standardisation principle and can be recommended for inclusion into the draft monograph «Valerian tincture».

Comparative analysis of the methods of quantitative determination of the total content of indole alkaloids

1996 ◽  
Vol 30 (4) ◽  
pp. 252-257
Author(s):  
Yu. V. Shelud'ko ◽  
I. M. Gerasimenko ◽  
I. A. Kostenyuk
Keyword(s):  
Comparative Analysis ◽  
Quantitative Determination ◽  
Indole Alkaloids ◽  
Total Content

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

Qualitative and quantitative determination of trace aldehydes and ketones in food preservative propionic acid for quality improvement

2021 ◽  
Author(s):  
Yiwen Cao ◽  
Shenjie Zhu ◽  
Lin Zhang ◽  
Qun Cui ◽  
Haiyan Wang
Keyword(s):  
Quality Improvement ◽  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Propionic Acid ◽  
Qualitative And Quantitative Analysis ◽  
Food Preservative ◽  
Qualitative And Quantitative ◽  
Aldehydes And Ketones ◽  
Aldehyde Content

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in...

scholarly journals Quantitative Analysis in Combination with Fingerprint Technology and Chemometric Analysis Applied for Evaluating Six Species of Wild Paris Using UHPLC-UV-MS

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yuangui Yang ◽  
Ji Zhang ◽  
Hang Jin ◽  
Jinyu Zhang ◽  
Yuanzhong Wang
Keyword(s):  
Principal Component Analysis ◽  
Quantitative Analysis ◽  
High Performance ◽  
Yunnan Province ◽  
Principal Component ◽  
Total Content ◽  
Similarity Analysis ◽  
Fast Method ◽  
Paris Polyphylla

A fast method was developed by ultra high performance liquid chromatography (UHPLC) for simultaneous determination of polyphyllin I and polyphyllin II. Chemometric analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) based on UHPLC chromatography were used to evaluate 38 batches from six species of Paris. Variable importance of projection was applied to select important peaks. Meanwhile, similarity analysis of UHPLC fingerprint was used to evaluate the sample of Paris polyphylla yunnanensis (PPY) and P. axialis (PA). The results indicated that the total content of saponins in PPY and PA collected from Baoshan City of Yunnan Province above 8.07 mg/g was stronger than that from other areas of the rest of species. PLS-DA showed better performance than PCA with regard to classifying the samples. Retention time during 20–27 minutes of UHPLC was screened as significant peak for distinguishing Paris of different species and original geography. All of PPY and PA with similarity value were more than 0.80. It indicated that quantitative analysis combined with chemometric and similarity analyses could evaluate the different species of Paris effectively and comprehensively.

More-sensitive enzyme-multiplied immunoassay technique for procainamide and N-acetylprocainamide in plasma, serum, and urine.

1988 ◽  
Vol 34 (5) ◽  
pp. 957-960 ◽  
Author(s):  
P R Henry ◽  
R A Dhruv
Keyword(s):  
Quantitative Analysis ◽  
Plasma Concentration ◽  
Controlled Release ◽  
Quantitative Determination ◽  
Dosage Forms ◽  
Clinical Samples ◽  
Immunoassay Technique ◽  
Analytical Recovery ◽  
Serum And Urine

Abstract A commercially available (Syva Co.) enzyme-multiplied immunoassay technique (EMIT) for the quantitative determination of procainamide (PA) and N-acetylprocainamide (NAPA) was modified to allow automated quantitative analysis of approximately 100 samples per day, in a working range of 0.1 to 2.0 micrograms/mL. Such a test was needed to evaluate the pharmacokinetic characteristics of controlled-release dosage forms characterized by long half-lives at low plasma concentration. Analytical recovery of PA and NAPA from serum, plasma, and urine was satisfactory, but at extreme ratios for PA:NAPA the accuracy of determining the lower-concentration component became unsatisfactory. In fact, however, we found no such ratios in 5400 clinical samples assayed by this procedure.

Quantitative Analysis of Flavonoids in the Flowers and Leaves of Ficaria verna Huds.

2003 ◽  
Vol 58 (9-10) ◽  
pp. 763-765 ◽  
Author(s):  
Michał Tomczyk ◽  
Jan Gudej
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Quantitative Determination ◽  
High Performance ◽  
Hplc Analysis ◽  
Flavonoid Content ◽  
Growing Seasons ◽  
Ficaria Verna

AbstractThe quantitative determination of flavonoid compounds in flowers and leaves of Ficaria verna Huds. (Ranunculaceae) was carried out in different growing seasons of the plant, using Christ-Müller’s method (Polish Pharmacopoeia, 1999) and high performance liquid chromatography (HPLC) analysis after acid hydrolysis. The flavonoid content was much higher in flowers than in leaves.

Quantitative determination of the neutral glycosyl ceramides in bovine thyroid gland

Biochimie ◽  
1977 ◽  
Vol 59 (10) ◽  
pp. 839-848 ◽  
Author(s):  
G. Van Dessel ◽  
A. Lagrou ◽  
H.J. Hilderson ◽  
W. Dierick ◽  
G. Dacremont
Keyword(s):  
Thyroid Gland ◽  
Quantitative Determination ◽  
Bovine Thyroid
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