scholarly journals Temporal expression of a PGIP-gene in strawberry cultivars induced by wounding or by Botrytis cinerea infection

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 504-506 ◽  
Author(s):  
L. Mehli
Keyword(s):  
Botrytis Cinerea ◽  
Developmental Regulation ◽  
Constitutive Expression ◽  
Comparative Experiment ◽  
Inhibitor Protein ◽  
Temporal Expression ◽  
Rt Pcr ◽  
Strawberry Cultivar ◽  
Strawberry Cultivars ◽  
Pgip Gene

The expression of a PGIP gene (polygalacturonase inhibitor protein) was monitored with semi-quantitative (SQ)-RT-PCR in green, white and red berries of the strawberry cultivar Korona upon infection with Botrytis cinerea and wounding. In addition, the PGIP expression in infected white berries was quantified in four additional cultivars. The constitutive expression of PGIP increased from green to red berries in Korona suggesting developmental regulation of the gene. Wounding and fungal infection caused a moderate or a high induction in the PGIP level, respectively. The maximum peak was observed 24 h after the treatments. In the comparative experiment with five cultivars, infection of white berries caused an induction in the PGIP level 24 h after inoculation in four out of five cultivars.

Strawberry Cultivar Performance and Susceptibility to Tarnished Plant Bug Injury

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460d-460
Author(s):  
D.T. Handley ◽  
M.A. Schupp ◽  
J.F. Dill
Keyword(s):  
Lower Order ◽  
Tarnished Plant Bug ◽  
Strawberry Cultivar ◽  
Marketable Fruit ◽  
Feeding Pressure ◽  
High Yields ◽  
Insecticide Sprays ◽  
Strawberry Cultivars

Twelve strawberry cultivars established in matted row plots in 1993 were treated with insecticides for tarnished plant bug or left untreated for the 1994, 1995, and 1996 seasons. `Honeoye', `Cavendish', and `Oka' had the highest yields of marketable fruit. `Jewel', `Chambly', and `Kent' had lower, but acceptable, yields. `Lateglow', `Blomidon', `Seneca, NY1424', `Settler', and `Governor Simcoe' had lower yields than other varieties. Tarnished plant bug populations were very low during the 1994 and 1996 seasons, and thus feeding pressure may have been too low for any differences in susceptibility between varieties to be expressed. In 1995, when tarnished plant bug feeding pressure was greatest, `Oka', `Cavendish', and `Honeoye' had the lowest injury levels. `Kent' and `Lateglow' had the highest levels of injury. Insecticide sprays significantly reduced the percent of injured fruit for most cultivars, but did not significantly increase the weight of marketable fruit harvested. This is due to injury being most prevalent on lower order, and thus smaller, fruit. Cultivars that produced high yields, had low injury levels, and had the least difference between sprayed and unsprayed treatments are most likely to have resistance to tarnished plant bug injury. `Oka', `Cavendish', and `Honeoye' were the most promising cultivars in this regard.

Fungicide Dip Treatments for Management of Botrytis cinerea Infection on Strawberry Transplants

2018 ◽  
Vol 19 (4) ◽  
pp. 279-283
Author(s):  
Michelle S. Oliveira ◽  
Leandro G. Cordova ◽  
Marcus V. Marin ◽  
Natalia A. Peres
Keyword(s):  
Botrytis Cinerea ◽  
Causal Agent ◽  
Fruit Rot ◽  
Plant Establishment ◽  
Fungicide Sensitivity ◽  
Strawberry Cultivars

Botrytis cinerea, the causal agent of Botrytis fruit rot, is annually introduced into Florida strawberry fields by infected transplants. The disease can be managed by fungicide sprays throughout the season; however, previous studies have demonstrated that several chemical classes are no longer effective owing to B. cinerea resistance. The objectives of this study were to evaluate the effectiveness of preplant fungicide-dip treatments of strawberry transplants on reducing B. cinerea colonization and to evaluate fungicide sensitivity of surviving B. cinerea isolates. Transplants of two strawberry cultivars, Winterstar ‘FL 05-107’ and ‘Florida Radiance’, were dipped in 11 different fungicides. After the plant establishment period, eight leaves per plot were collected before and 14 days after treatment to evaluate B. cinerea incidence. Isolates (n = 139) obtained from the transplants were tested for fungicide sensitivity. Plant diameter was measured 47 days after planting. All treatments, including the controls, reduced B. cinerea incidence by at least 77% compared with predip incidence. However, fungicide-resistant isolates were recovered from all treatments tested.

scholarly journals Human mononuclear cells express 12-LX: coordinated mRNA regulation with 5-LX and FLAP genes

Blood ◽  
1996 ◽  
Vol 87 (1) ◽  
pp. 331-340
Author(s):  
WE Kaminski ◽  
E Jendraschak ◽  
K Baumann ◽  
R Kiefl ◽  
S Fischer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Time Course ◽  
Cell Activation ◽  
Mononuclear Cells ◽  
Constitutive Expression ◽  
Ionophore A23187 ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Human Mononuclear Cells

Lipoxygenases (LXs) catalyze formation of leukotrienes and hydroxy-eicosatetraenoic acids (HETEs), proinflammatory, and spasmogenic autacoids that are critical for host defense systems. We studied the expression and regulation of LX genes (12-LX, 5-LX, and 15-LX) and the 5-lipoxygenase activating protein (FLAP) in human mononuclear cells (MNC) and granulocytes using a quantitative reverse transcription polymerase chain reaction (RT-PCR) technique. We show that 12-LX mRNA is constitutively expressed in resting platelet-free MNC. 12-LX gene expression was upregulated by activation with lipopolysaccharide (LPS). The formation of 12-HETE was inducible with ionophore in MNC, as assessed by high-performance liquid chromatography (HPLC) and gas chromatography, and increased after LPS pretreatment. In addition to 12- LX, resting MNC expressed the genes for 5-LX and FLAP constitutively. Quantitative time course analyses of 12-LX, 5-LX, and FLAP gene expression suggested coregulation of 12-LX and FLAP mRNAs, and reciprocal regulation of 5-LX and FLAP mRNAs. During cell stimulation with LPS 5-LX mRNA levels remained unchanged, whereas FLAP gene expression increased. No 15-LX mRNA expression or 15-HETE formation was detectable in unstimulated and activated MNC. In contrast to MNC, quantitative RT-PCR mRNA analysis showed intermittent intraindividual expression of the 5-LX and FLAP genes in resting granulocytes. mRNAs for 12-LX and 15-LX were not expressed. On stimulation of granulocytes ex vivo, mRNA expression of 5-LX and FLAP was upregulated. Stimulation by LPS differed from that by ionophore A23187. Neither LPS nor ionophore induced gene expression of 12-LX or 15-LX in granulocytes. Our data indicate that resting human MNC and granulocytes express LX and FLAP genes in a cell-specific manner. Cell activation induces coordinated upregulation of 12-LX and FLAP genes in MNC, and 5-LX and FLAP genes in granulocytes, respectively. The constitutive expression of 12-LX mRNA, its upregulation on cell activation, and the formation of 12-HETE clearly indicate the presence of a functional 12-LX in human MNC.

scholarly journals CHROMOSOME NUMBER AND TIME PERIOD OF MITOTIC CYCLE OF FESTIVAL AND CALIFORNICA STRAWBERRY CULTIVARS (Fragaria x ananassa AND Fragaria vesca)

2015 ◽  
Vol 2 (1) ◽  
pp. 476
Author(s):  
Ganies Riza Aristya ◽  
Rezika Alyza ◽  
Rosyidatul Khoiroh ◽  
Budi Setiadi Daryono
Keyword(s):  
Chromosome Number ◽  
Reverse Genetics ◽  
Fragaria X Ananassa ◽  
Fragaria Vesca ◽  
Time Period ◽  
Genomics Research ◽  
Strawberry Cultivar ◽  
Pre Treatment ◽  
Seed Propagation ◽  
Strawberry Cultivars

<p>The cultivated strawberries, Fragaria x ananassa and Fragaria vesca, are the most economically-important softfruit species. F x ananassa and F vesca, both diploid (2n=2x=14) relatives of the commercial octoploid strawberry, are an attractive model for functional genomics research in Rosaceae. Its small genome size, short reproductive cycle, and facile vegetative and seed propagation make F. x annassa and F.vesca a promising candidates for forward and reverse genetics experiments. In order to determine their genetic differences in more detail, chromosome characterization of the two strawberry cultivars was investigated. A method used for chromosome slides in this research was a squash method with modification in pre-treatment. The result showed Fragaria x ananassa had (2n = 4x = 28) chromosome number is 28 and Fragaria vesca had (2n = 2x = 14) chromosome number is 14. The time of mitotic that both strawberry cultivars was similar at 7 to 8.30 am. In addition, mixoploid cells were found in both strawberry cultivar indicating that these cultivars had been treated by mutagenic agents for a breeding program.</p><p><br /><strong>Keywords</strong> : Fragaria, chromosome, mitotic</p>

scholarly journals Human mononuclear cells express 12-LX: coordinated mRNA regulation with 5-LX and FLAP genes

Blood ◽  
1996 ◽  
Vol 87 (1) ◽  
pp. 331-340 ◽  
Author(s):  
WE Kaminski ◽  
E Jendraschak ◽  
K Baumann ◽  
R Kiefl ◽  
S Fischer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Time Course ◽  
Cell Activation ◽  
Mononuclear Cells ◽  
Constitutive Expression ◽  
Ionophore A23187 ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Human Mononuclear Cells

Abstract Lipoxygenases (LXs) catalyze formation of leukotrienes and hydroxy-eicosatetraenoic acids (HETEs), proinflammatory, and spasmogenic autacoids that are critical for host defense systems. We studied the expression and regulation of LX genes (12-LX, 5-LX, and 15-LX) and the 5-lipoxygenase activating protein (FLAP) in human mononuclear cells (MNC) and granulocytes using a quantitative reverse transcription polymerase chain reaction (RT-PCR) technique. We show that 12-LX mRNA is constitutively expressed in resting platelet-free MNC. 12-LX gene expression was upregulated by activation with lipopolysaccharide (LPS). The formation of 12-HETE was inducible with ionophore in MNC, as assessed by high-performance liquid chromatography (HPLC) and gas chromatography, and increased after LPS pretreatment. In addition to 12- LX, resting MNC expressed the genes for 5-LX and FLAP constitutively. Quantitative time course analyses of 12-LX, 5-LX, and FLAP gene expression suggested coregulation of 12-LX and FLAP mRNAs, and reciprocal regulation of 5-LX and FLAP mRNAs. During cell stimulation with LPS 5-LX mRNA levels remained unchanged, whereas FLAP gene expression increased. No 15-LX mRNA expression or 15-HETE formation was detectable in unstimulated and activated MNC. In contrast to MNC, quantitative RT-PCR mRNA analysis showed intermittent intraindividual expression of the 5-LX and FLAP genes in resting granulocytes. mRNAs for 12-LX and 15-LX were not expressed. On stimulation of granulocytes ex vivo, mRNA expression of 5-LX and FLAP was upregulated. Stimulation by LPS differed from that by ionophore A23187. Neither LPS nor ionophore induced gene expression of 12-LX or 15-LX in granulocytes. Our data indicate that resting human MNC and granulocytes express LX and FLAP genes in a cell-specific manner. Cell activation induces coordinated upregulation of 12-LX and FLAP genes in MNC, and 5-LX and FLAP genes in granulocytes, respectively. The constitutive expression of 12-LX mRNA, its upregulation on cell activation, and the formation of 12-HETE clearly indicate the presence of a functional 12-LX in human MNC.

scholarly journals Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Chirag Nepal ◽  
Yavor Hadzhiev ◽  
Piotr Balwierz ◽  
Estefanía Tarifeño-Saldivia ◽  
Ryan Cardenas ◽  
...  
Keyword(s):  
Developmental Regulation ◽  
Fruit Fly ◽  
Temporal Expression ◽  
Transcription Start ◽  
Transcription Start Sites ◽  
Promoter Architecture ◽  
Transcript Processing ◽  
Spatio Temporal ◽  
Host Genes ◽  
Dual Initiation

AbstractVariations in transcription start site (TSS) selection reflect diversity of preinitiation complexes and can impact on post-transcriptional RNA fates. Most metazoan polymerase II-transcribed genes carry canonical initiation with pyrimidine/purine (YR) dinucleotide, while translation machinery-associated genes carry polypyrimidine initiator (5’-TOP or TCT). By addressing the developmental regulation of TSS selection in zebrafish we uncovered a class of dual-initiation promoters in thousands of genes, including snoRNA host genes. 5’-TOP/TCT initiation is intertwined with canonical initiation and used divergently in hundreds of dual-initiation promoters during maternal to zygotic transition. Dual-initiation in snoRNA host genes selectively generates host and snoRNA with often different spatio-temporal expression. Dual-initiation promoters are pervasive in human and fruit fly, reflecting evolutionary conservation. We propose that dual-initiation on shared promoters represents a composite promoter architecture, which can function both coordinately and divergently to diversify RNAs.

Growth Suppressors IFI-204 and IFI-205 Inhibit Primitive Hematopoietic Cell Proliferation In Vitro and in Vivo.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1691-1691
Author(s):  
Kimberly Klarmann ◽  
Daniel Gough ◽  
Benyam Asefa ◽  
Chris Clarke ◽  
Katie Renn ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Growth ◽  
Cell Line ◽  
Constitutive Expression ◽  
Rt Pcr ◽  
Hematopoietic Stem ◽  
Inhibit Cell Growth ◽  
Stem And Progenitor Cells

Abstract Members of the interferon inducible-200 (IFI-200) family of proteins inhibit cell growth and may be important mediators of differentiation. We examined IFI-204 and IFI-205 mRNA expression in purified populations of hematopoietic stem and progenitor cells at different stages of maturation using quantitative RT-PCR and found that their expression markedly increased during myeloid maturation. To evaluate the effect of IFI-205 and IFI-204 on hematopoietic stem cell (HSC) growth, we transduced these genes into mouse bone marrow cells (BMC) using retroviral vectors. The presence IFI-204 or IFI-205 resulted in a decrease in cell growth in response to hematopoietic growth factors. Further analysis revealed the infected cells were 98% c-Kit+ Sca-1+, indicative of the stem cell surface phenotype, suggesting they may be blocked in a primitive stage of maturation. When transplanted, BMC transduced with IFI-204 or IFI-205 failed to engraft lymphoid, myeloid, or erythroid lineages in both short and long term reconstitution assays, suggesting that constitutive expression of IFI-204 and IFI-205 inhibited HSC development both in vitro and in vivo. However, based on the quantitative RT-PCR results, which show that IFI-205 increased during myeloid differentiation, we know its endogenous, regulated expression must permit the cells to mature. Therefore, to study of the effects of these genes on differentiation we transduced the mulitpotential EML (erythroid, myeloid, lymphoid) cell line with IFI-204 and IFI-205 to circumvent severe growth inhibition caused by expression of IFI-204 and Ifi-205 in normal cells. Single cell analysis of EMLs transduced with IFI-205 demonstrated that expression of IFI-205 in this cell line did not significantly inhibit cell growth. We have isolated EML clones from the transduced cells and verified IFI-205 expression. In addition, we generated transgenic mice that express IFI-205 under control of the Vav and MRP8 promoters, and we identified transgenic lines that express IFI-205 at higher levels compared to wild type controls. Analysis of hematopoiesis in these animals is currently in progress. Altogether, our data demonstrate 3 findings: 1) IFI-204 and IFI-205 expression increases during myeloid development based on quantitative RT-PCR analysis, 2) constitutive expression of IFI-204 and -205 results in potent inhibition of growth and maturation of normal hematopoietic stem and progenitor cells in vivo and in vitro and 3) these genes did not significantly inhibit the proliferation of the EML cell line, which provides us with a means to study the mechanism by which these molecules regulate myeloid maturation. Finally, the considerable inhibitory effects of these family members on normal hematopoietic cell growth suggest their potential as therapeutic modalities for treatment of leukemia.

scholarly journals Relationship between production, nematodes and "redness" in strawberries

2016 ◽  
Vol 46 (8) ◽  
pp. 1309-1315 ◽  
Author(s):  
Paula Nogueira Curi ◽  
Pedro Maranha Peche ◽  
Rafael Pio ◽  
Csaignon Mariano Caproni ◽  
Márcia Souza de Oliveira
Keyword(s):  
Botrytis Cinerea ◽  
Fruit Quality ◽  
Fruit Production ◽  
Causal Agent ◽  
Meloidogyne Hapla ◽  
Plant Survival ◽  
Camino Real ◽  
High Incidence ◽  
The Relationship ◽  
Strawberry Cultivars

ABSTRACT: In recent years "redness" has increasingly appeared in strawberry plants with leaves taking on a reddish color. No causal agent has been associated with plants. Since strawberries presented problems due to the incidence of nematodes, the purpose of this study was to look at the relationship between production, resistance to the Meloidogyne hapla nematode and the "redness" symptom in strawberry cultivars. Two experiments were performed, both with the 'Camino Real', 'Festival', 'Oso Grande', 'Albion' and 'Camarosa' cultivars. The first experiment was performed in the field, where the following were evaluated: strawberry production, fruit quality, macro and micronutrient contents in fruit and leaves, percentage of plant survival, incidence of nematodes, quantity of eggs in the roots and juveniles in the soil, and the incidence of Botrytis cinerea . In the second experiment, the strawberries were transplanted into pots and filled with pinus bark-based commercial substrate. Half the pots were inocculated with Meloidogyne hapla . Cultivars presented differences in fruit production and also in the incidence of "redness". Lowest performance in production was related to the high incidence of the nematode Meloidogyne hapla. 'Oso Grande' and 'Albion' presented nematode-resistant behavior. It was possible find a relationship between the incidence of the Meloidogyne hapla nematode, and the incidence of "redness" only 'Camino Real' cultivar.

scholarly journals Strawberry Cultivar Injury After Two Contrasting Minnesota Winters

2009 ◽  
Vol 19 (4) ◽  
pp. 803-808 ◽  
Author(s):  
Shengrui Yao ◽  
James J. Luby ◽  
David K. Wildung
Keyword(s):  
Snow Cover ◽  
Weather Conditions ◽  
Winter Hardiness ◽  
Severe Winter ◽  
Straw Mulch ◽  
Grand Rapids ◽  
Soil Temperatures ◽  
Strawberry Cultivar ◽  
Plant Stand ◽  
Strawberry Cultivars

As part of our hardy strawberry (Fragaria ×ananassa) breeding program, winter hardiness of 15 strawberry cultivars was evaluated in the field after Winter 2005–2006 and a test Winter 2006–2007 with no snow cover at Grand Rapids, MN. After the snow-covered Winter 2005–2006, plant stand (percent leaf coverage for the designated area for each plot) increased for all cultivars in the mulched treatment and some cultivars in the unmulched treatment with slight decreases only for several cultivars in the unmulched treatment. However, after Winter 2006–2007, the plant stands of all cultivars drastically decreased in both mulched and unmulched treatments. ‘Clancy’, ‘Evangeline’, and ‘L'Amour’ were the three most sensitive cultivars among the 15 cultivars tested. ‘Kent’, ‘Mesabi™’, ‘Cavendish’, and ‘Brunswick’ were the highest yielding cultivars for both 2006 and 2007 in the mulched treatment. In the unmulched treatment, ‘Brunswick’, ‘Mesabi™ ’, ‘Cavendish’, ‘Sable’, and ‘Kent’ were the top yielding cultivars after Winter 2006–2007. During Winter 2005–2006, with 20 to 30 cm snow cover throughout the season, the 5- and 10-cm soil temperatures remained constant at ≈30 to 31.5 °F in both mulched and unmulched treatments. In contrast, during Winter 2006–2007, there were 16 and 24 days (consecutive) in February below 18 °F at 5-cm soil depths for mulched and unmulched treatments, respectively, which probably led to the severe winter damage. Although straw mulch afforded the plants some protection, snow cover is critical to the survival of strawberries in northern Minnesota and other areas with similar weather conditions.

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