scholarly journals Occurrence of biogenic amines and amines degrading bacteria in fish sauce

2010 ◽  
Vol 28 (No. 5) ◽  
pp. 440-449 ◽  
Author(s):  
M.Z. Zaman ◽  
F.A. Bakar ◽  
J. Selamat ◽  
J.. Bakar

The contents of biogenic amines histamine, putrescine, and cadaverine in fish sauce were determined and the bacteria isolated from the samples were evaluated for their amines degradation activity. Five fish sauce samples contained 62.5–393.3 ppm of histamine, 5.6–242.8 ppm of putrescine, and 187.1–704.7 ppm of cadaverine. Thirty three bacterial isolates produced all three amines, seven isolates produced one or two amines, and one isolate did not produce any amine in differential agar media. Since the strains that produced amines were not supposed to degrade them, only eight isolates were further identified and evaluated for their amines degrading capability. Bacillus amyloliquefaciens FS-05 and Staphylococcus carnosus FS-19 degraded histamine up to 59.9% and 29.1% from its initial concentration, respectively. Staphylococcus intermedius FS-20 and Bacillus subtilis FS-12 degraded putrescine and cadaverine up to 30.4% and 28.9%, respectively. Most isolates tolerated the salt concentration of up to 15% and temperature of up to 45°C. The current study provided new information on biogenic amines degrading bacteria, isolated from high-salt-content food products. The amines degradation activity of the bacteria is considered as strain rather than species specific.

1991 ◽  
Vol 54 (6) ◽  
pp. 424-428 ◽  
Author(s):  
LAURA L. ZAIKA ◽  
ANNA H. KIM ◽  
LOUISE FORD

A partial factorial design study of the effect of NaNO2 (0, 100, 200, 1000 ppm) in combination with NaCl (0.5, 2.5, 4.0%), pH (7.5, 6.5, 5.5), and temperature (37, 28, 19°C) on growth of Shigella flexneri is reported. Experiments were done aerobically in brain-heart infusion medium, using an inoculum of 1 × 103 CFU/ml. Growth curves were fitted from plate count data by the Gompertz equation; exponential growth rates, lag times, generation times, and maximum populations were derived for all variable combinations. In the absence of nitrite, the organism grew well under all test conditions at 37 and 28°C but did not grow at 19°C at pH 5.5 nor at pH 7.5 with 4% NaCl. Nitrite did not affect growth in media of pH 7.5 at 37 and 28°C. At pH 6.5 growth was inhibited by 1000 ppm NaNO2. The organism failed to grow at 19°C at all nitrite levels in the presence of 2.5 or 4.0% NaCl. The inhibitory effect of nitrite was much greater in media of pH 5.5 and increased with increasing salt levels. More inhibition was apparent at 28 than at 37°C. While lack of growth was used as a paradigm of the effect of nitrite on S. flexneri, nitrite also increased the lag and generation times and decreased the exponential growth rate. Results indicated that NaNO2 in combinations with low temperature, low pH, and high salt content can effectively inhibit the growth of S. flexneri.


2021 ◽  
Vol 25 (7) ◽  
pp. 8-12
Author(s):  
Z.G. Lamerdonov ◽  
T.Yu. Khashirova ◽  
S.A. Zhaboev ◽  
L.Zh. Nastueva ◽  
A.А. Shogenov ◽  
...  

The results of experimental studies of the local subsurface irrigation method in comparison with drip irrigation carried out in the laboratory, which showed water savings due to a decrease in evaporation from the soil surface by 10–15 percent are presented. The method of irrigation in closed greenhouse farms using water with a high salt content is described. The paper proposes new patented schematic solutions for protecting plants from frost and pests, describes a multifunctional engineering and reclamation system capable of performing various operations depending on the emerging problems during the growing season.


2020 ◽  
Vol 5 (4(73)) ◽  
pp. 12-18
Author(s):  
G.T. Bekmirzaev ◽  
I.A. Begmatov ◽  
D.B. Yulchiev

The purpose of the experimental study was the selection of salt tolerant crops and the search for useful horticultural species for growing them on saline lands. The experimental study was conducted at the University of Algarve, Portugal, in a greenhouse. The following vegetable crops were selected for research: lettuce (Lactuca sativaL), New Zealand spinach (Tetragonia tetragonioides) and garden purslane (Portulaca oleracea). Experimental results showed that New Zealand spinach and garden purslane have high potential as species resistant to high salt content and are therefore recommended for cultivation in order to reduce soil salinity. The above crops, mainly New Zealandspinach, are good types of garden crops with high useful qualities and productivity. Therefore, it has been shown that this method is a clean and environmentally friendly tool to prevent salinization and maintain the sustainability of agricultural systems


2018 ◽  
Vol 777 ◽  
pp. 554-558
Author(s):  
Vu Hai Dang ◽  
Manoon Masniyom

The effect of the high salinity water on the compressive strength of mine backfill was studied. Two types of salinity water: saturated and unsaturated brines were employed to mix with mine backfill materials, and the results were compared. The one with saturated brine had high salt content of 400 g/l while the other had 200 g/l. The results showed that compressive strength decreased with increasing salt content. The mine backfill with high salt content (saturated brine) exhibited the poorest compressive strength in which its strength decreased to approximately 50-70 % of the original strength gained from the backfill samples based on water without salt. Additionally, the optimal saline water solid ratio was 0.2.


2019 ◽  
Vol 102 (1) ◽  
pp. 164-180 ◽  
Author(s):  
Kazuhiko Shimoji ◽  
Mikio Bakke ◽  
James M Hungerford ◽  
Christina A Mireles DeWitt ◽  
Sevim Köse

Abstract Background: Since prescribed limits for histamine in fish have been set by various regulatory bodies around the world, the rapid, specific and easy determination of histamine is in high demand. Objective: The enzymatic histamine assay developed by Kikkoman Biochemifa Co. was validated. Methods: Fresh and frozen raw tuna, canned tuna in oil and water, and anchovy fish sauce were used for the validation study under the specific guidelines of the AOAC Research Institute Performance Tested MethodSM (PTM) program. Results: Good linearity (R2 > 0.9997) for histamine standard solutions, recovery rates (90.3–125.2%), and repeatability precisions (RSDr < 10%) were shown for all spiked tested matrixes. The claimed LOQ values, 20 and 10 mg/kg for solid fish and 160 and 80 mg/kg for fish sauce using a 1 and 2 cm optical path length spectrophotometer, respectively, were validated. The cross-reactivity test showed no positive interference of other biogenic amines, except for agmatine and putrescine. Moreover, no inhibition was confirmed among the 12 biogenic amines. Stability data supported the shelf life (42 months at 4°C), lot-to-lot consistency was demonstrated, and the method was shown to be robust. Independent laboratory testing using canned tuna in oil demonstrated that the recovery ranged from 93.5 to 124.7%, and the RSDr at all spike levels was <20%. Conclusions: The simple and rapid enzymatic histamine assay method has been successfully validated. Highlights: This histamine quantitative assay kit was qualified for PTM certification No. 041802.


2020 ◽  
Vol 43 ◽  
pp. e50468
Author(s):  
Mirna Clarissa Rodrigues de Almeida ◽  
Mauricio Luiz de Mello Vieira Leite ◽  
Luciana Sandra Bastos de Souza ◽  
Vicente José Laamon Pinto Simões ◽  
Luiz Guilherme Medeiros Pessoa ◽  
...  

Agricultural production in semi-arid regions is limited due to water availability. In addition, the water quality available for irrigation is often compromised due to the high salt content present. Millet is a forage species considered tolerant to water deficit and moderately salt tolerant. In view of the above, the objective was to evaluate the growth of millet under water and saline stress associates. The experiment was carried out in a randomized block design, in a 4x3 factorial scheme, composed of four levels of water replacement, based on crop evapotranspiration (ETc): 25%.ETc, 50%.ETc, 75%.ETc and 100%.ETc and three levels of water salinity (0.03, 2.0 and 4.0 dS m-1). With 25%.ETc independent of salinity, all morphological characteristics of millet were affected, occurring death of plants in the initial growth phase. In the absence of salt and greater availability of water, greater plant growth occurred. With respect to salinity, there was a reduction in the increment of all variables evaluated, with the highest reduction at the highest saline level (4.0 dS m-1). Water and salt stresses, when associated, reduce the growth of millet, since concentrations above 2.0 dS m-1 and less than 50%.ETc compromise its full development, providing declines in yield.


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