scholarly journals Effect of nitrogen sources on the nitrogenous forms and accumulation of amino acid in head cabbage

2008 ◽  
Vol 54 (No. 2) ◽  
pp. 66-71 ◽  
Author(s):  
E. Atanasova
Keyword(s):  
Amino Acids ◽  
Ammonium Nitrate ◽  
Nitrogen Nutrition ◽  
Calcium Nitrate ◽  
Total Content ◽  
Nitrogen Sources ◽  
Nitrogen Accumulation ◽  
Protein Nitrogen ◽  
Nitrogen Rates ◽  
Head Cabbage

The effect of two different nitrogen sources: ammonium nitrate and calcium nitrate, applied at increased rates, on the content of the total nitrogen, protein and non-protein nitrogen, and the content and composition of amino acids in head cabbage leaves was studied. The higher nitrogen accumulation was established at the ammonium nitrate fertilization compared to the calcium nitrate, but the application of Ca(NO<sub>3</sub>)<sub>2</sub> resulted in a higher content of nitrate nitrogen. More protein nitrogen was also observed in plants with Ca(NO<sub>3</sub>)<sub>2</sub> fertilization. The highest applied fertilizer rate of ammonium nitrate resulted in a significant reduction of the protein nitrogen compared to increased free amino acids. The total content of amino acids increased gradually with the increase of nitrogen rates in plants fertilized with NH<sub>4</sub>NO<sub>3</sub>. When Ca(NO<sub>3</sub>)<sub>2</sub> was applied, nitrogen rates higher than 500 mg/kg soil suppressed the synthesis of amino acids. A greater increase was observed as regards the levels of arginine, proline and some essential amino acids as lysine, phenylalanine and histidine. The increase of proline and alanine could serve as an indicator for unbalanced nitrogen nutrition.

scholarly journals Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose

2020 ◽  
Vol 8 (6) ◽  
pp. 799
Author(s):  
Emilio M. Ungerfeld ◽  
M. Fernanda Aedo ◽  
Camila Muñoz ◽  
Natalie L. Urrutia ◽  
Emilio D. Martínez ◽  
...  
Keyword(s):  
Amino Acids ◽  
De Novo ◽  
Nitrogen Sources ◽  
Environmental Benefits ◽  
De Novo Synthesis ◽  
Protein Nitrogen ◽  
Protein Supplements ◽  
Batch Cultures ◽  
Sufficient Energy ◽  
Ruminant Diets

Ameliorating methane (CH4) emissions from ruminants would have environmental benefits, but it is necessary to redirect metabolic hydrogen ([H]) toward useful sinks to also benefit animal productivity. We hypothesized that inhibiting rumen methanogenesis would increase de novo synthesis of microbial amino acids (AA) as an alternative [H] sink if sufficient energy and carbon are provided. We examined the effects of inhibiting methanogenesis with 9, 10-anthraquione (AQ) on mixed rumen batch cultures growing on cellulose or starch as sources of energy and carbon contrasting in fermentability, with ammonium (NH4+) or trypticase (Try) as nitrogen (N) sources. Inhibiting methanogenesis with AQ inhibited digestion with cellulose but not with starch, and decreased propionate and increased butyrate molar percentages with both substrates. Inhibiting methanogenesis with 9, 10-anthraquinone increased de novo synthesis of microbial AA with starch but not with cellulose. The decrease in the recovery of [H] caused by the inhibition of methanogenesis was more moderate with starch due to an enhancement of butyrate and AA as [H] sinks. There may be an opportunity to simultaneously decrease the emissions of CH4 and N with some ruminant diets and replace plant protein supplements with less expensive non-protein nitrogen sources such as urea.

Nitrogen nutrition and regulation of cephalosporin production in Streptomyces clavuligerus

1979 ◽  
Vol 25 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Yair Aharonowitz ◽  
Arnold L. Demain
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Nitrogen Nutrition ◽  
Antibiotic Production ◽  
Inorganic Nitrogen ◽  
Amino Nitrogen ◽  
Nitrogen Sources ◽  
Streptomyces Clavuligerus ◽  
Defined Medium ◽  
Repressive Effect

When used as sole nitrogen source, certain amino acids (e.g., proline, asparagine) supported both growth and sporulation by Streptomyces clavuligerus streaked onto solid defined medium. Ammonium supported growth but suppressed sporulation. Amino nitrogen was best for cephalosporin production in liquid defined medium, although urea was almost as useful. A comparison of amino acids showed asparagine and glutamine to be the best nitrogen sources and arginine to be almost as good. Ammonium salts supported a somewhat lower growth rate than asparagine, but antibiotic production was very poor on these inorganic nitrogen sources. Addition of ammonium to asparagine did not affect growth rate but increased mycelial mass; cephalosporin production was reduced by about 75%. Antibiotic production was more closely associated with growth in the absence of ammonium than in its presence, indicating a strong inhibitory and (or) repressive effect of NH4+ on antibiotic production. Ammonium exerted its negative effect when added at 24 h or earlier, i.e. before antibiotic formation began.

scholarly journals Nitrate and Ammonium Leaching Losses from N Fertilizers Applied to `Penncross' Creeping Bentgrass

HortScience ◽  
1990 ◽  
Vol 25 (2) ◽  
pp. 194-196 ◽  
Author(s):  
Charles F. Mancino ◽  
Joseph Troll
Keyword(s):  
Ammonium Nitrate ◽  
Urea Formaldehyde ◽  
Peat Soil ◽  
Calcium Nitrate ◽  
Nitrogen Sources ◽  
Creeping Bentgrass ◽  
Ammonium Nitrogen ◽  
Putting Greens ◽  
Leaching Losses ◽  
Greenhouse Study

Combining frequent N applications and irrigations for turfgrasses grown in sandy soils is a common occurrence on golf course putting greens. A greenhouse study was conducted to determine leaching losses of nitrate and ammonium nitrogen from `Penncross' creeping bentgrass (Agrostis palustris L.) growing on an 80 sand:20 peat soil mixture following frequent, moderately heavy irrigations and light or moderate N fertilizer applications. Nitrogen sources included calcium nitrate, ammonium nitrate, ammonium sulfate, urea, urea formaldehyde and isobutylediene diurea. Application levels were 9.76 kg N/ha per 7 days and 19.52 kg N/ha per 14 days for 10 weeks. Irrigation equivalent to 38 mm·week-1 was applied in three equal applications. Overall, 46% of the applied water leached. Total leaching losses were <0.5% of the applied N. Nitrate represented the major portion of the leached N, with ammonium losses being negligible. There were no differences between sources when applied at these levels. In a second study, a single 48.8 kg N/ha application resulted in higher leaching losses of N, but only calcium nitrate and ammonium nitrate had total losses > 2% (2.80% and 4.13%, respectively, over an n-day period). Nitrate concentrations were found to exceed 45 mg·liter-1 for ammonium nitrate.

Field experiments comparing ammonium sulphate, ammonium nitrate, calcium nitrate and urea combine-drilled with spring barley

1963 ◽  
Vol 61 (3) ◽  
pp. 381-390 ◽  
Author(s):  
J. R. Devine ◽  
M. R. J. Holmes
Keyword(s):  
Ammonium Nitrate ◽  
Ammonium Sulphate ◽  
Field Experiments ◽  
Spring Barley ◽  
Calcium Nitrate ◽  
Nitrogen Sources ◽  
Early Growth ◽  
Plant Population ◽  
Nitrogen Fertilizers ◽  
Grain Yields

1. Twenty-one experiments were carried out in various parts of England and Scotland in 1959–61 comparing two or more of the nitrogen sources ammonium sulphate, ammonium nitrate, calcium nitrate and urea, combine-drilled in compound fertilizers for spring barley.2. Ammonium sulphate and ammonium nitrate combine-drilled at rates from 35 to 105 lb./acre of nitrogen checked early growth slightly in some of the experiments, with no important difference between the two sources, which also gave similar grain yields.3. Calcium nitrate and urea combine-drilled at 45 lb./acre of nitrogen had no large effect on early growth, while at 70 and 90 lb./acre both fertilizers seriously delayed brairding and reduced the plant population in many of the experiments, especially in eastern England. They gave lower yields than ammonium sulphate and ammonium nitrate in many of the experiments in which early growth was affected, and gave lower mean yields at all rates of application.4. In eleven of the experiments, broadcast applications of two or more of the four nitrogen fertilizers were compared. All sources gave similar mean yields.5. There was a slightly smaller yield from combine drilling than from broadcasting ammonium sulphate and ammonium nitrate, and a markedly smaller yield from calcium nitrate and urea.

scholarly journals The contents of amino acids and sterols in maize plants growing under different nitrogen conditions

2010 ◽  
Vol 56 (No. 3) ◽  
pp. 125-132 ◽  
Author(s):  
M. Pavlík ◽  
D. Pavlíková ◽  
J. Balík ◽  
M. Neuberg
Keyword(s):  
Amino Acids ◽  
Ammonium Nitrate ◽  
Aboveground Biomass ◽  
Plant Biomass ◽  
Nitrogen Nutrition ◽  
Nitrate Solution ◽  
Amino Acid Content ◽  
Membrane Properties ◽  
Ammonium Nitrate Solution ◽  
Maize Plants

The effect of nitrogen nutrition on phytosterol and amino acid content in aboveground biomass of maize (<I>Zea mays</I> L.) was investigated in a pot experiment. For cultivation of maize plants nitrogen dose (2 or 4 g N/pot) was applied in the form of ammonium nitrate (AN) for control treatments or urea ammonium nitrate solution (UAN). UAN solution was applied according to the CULTAN method (Controlled Uptake Long Term Ammonium Nutrition). The content of amino acids as important nitrogen-containing compounds in plant biomass was affected by nitrogen nutrition. An increase of glutamine and asparagine levels in maize aboveground biomass was observed after UAN solution application. The results of free β-sitosterol analyses by HPLC showed its 94% increasing concentration after UAN application in contrast to AN treatments. Our results confirmed that sterol interconversions are controlled by environmental conditions and they are involved in the regulation of membrane properties in response to changing growth conditions.

Effects of Nitrogen Source and Concentration on the Free Amino Acid Composition of Developing Wheat Grains

1990 ◽  
Vol 17 (2) ◽  
pp. 199 ◽  
Author(s):  
C Blumenthal ◽  
JW Lee ◽  
EWR Barlow ◽  
IL Batey
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ammonium Nitrate ◽  
Amino Acid Composition ◽  
Free Amino Acid ◽  
Acid Composition ◽  
Free Amino ◽  
Amino Nitrogen ◽  
Grain Protein ◽  
Protein Nitrogen

Detached wheat heads (7 days post-anthesis) were grown in liquid culture containing nitrogen concentrations of 0.025% or 0.1% in the form of glutamine, ammonium nitrate or asparagine. With each form of the nitrogen, increasing the concentrations of nitrogen in the culture medium led to increases in the total nitrogen and the non-protein nitrogen in the grain. Protein contents (N × 5.7) were approximately 16% and 21% on a dry weight basis in the low and high treatments respectively for all nitrogen sources. Amino acids from the endosperm cavity, the ethanol-soluble extract of the grain, and the grain protein were analysed by HPLC techniques to define the site of transfer between amino acid forms. The results indicated that amino nitrogen from glutamine, ammonium nitrate, or asparagine enters the grain and is found in the endosperm cavity fluid mainly in the form of glutamine, alanine and, to a lesser extent, aspartate (including asparagine). These amino acids are then converted into the various other amino acids required for protein synthesis, as is demonstrated by the increases found in the others in the ethanol-soluble free amino acid fraction with different nitrogen regimes. These variations in the composition of the free amino acids occurred without altering the amino acid composition of the protein component of the grain.

GRAIN QUALITY FORECASTING BY AMINO ACID CONCENTRATION IN THE LEAF JUICE

2021 ◽  
pp. 29-41
Author(s):  
N.N. NOVIKOV ◽  
◽  
A.A. ZHARIKHINA ◽  
N.E. SOLOVYEVA
Keyword(s):  
Amino Acids ◽  
Grain Quality ◽  
Field Experiments ◽  
Nitrogen Nutrition ◽  
Total Content ◽  
Water Soluble ◽  
Soft Wheat ◽  
Malting Barley ◽  
Stem Node ◽  
Leaf Juice

Field experiments with soft wheat and malting barley, conducted on sod-podzolic medium loamy soil showed that under the influence of increasing rates of nitrogen nutrition in the leaf juice in the first stem node phase, the concentration of amino acids decreases. This fact is confirmed by high correlation coefficients. There is also a close correlation between the concentration of amino acids in the leaf juice, plant productivity and grain quality indicators. Wheat showed a close negative correlation of the concentration of amino acids in the leaf juice with the weight of 1000 grains, the total content of proteins and gluten in the grain, as well as gliadin and glutenin proteins, and a close positive correlation with the content of water-soluble, non-extractable proteins in the grain and the activity of proteases. The concentration of amino acids in the malting barley leaf juice was negatively correlated with the total content of proteins in the grain, the amount of gordeins, the total activity of amylases, and positively correlated with the test value indicators, grain extractivity, and the content of water-soluble proteins in the grain. The research results indicate that the concentration of amino acids in the leaf juice in the first stem node phase provides for a fairly accurate diagnoctics of nitrogen nutrition and prediction of the quality of soft wheat and malting barley grains.

The effect of different nitrogen sources on pigment production and sporulation of Monascus species in submerged, shaken culture

1977 ◽  
Vol 23 (10) ◽  
pp. 1360-1372 ◽  
Author(s):  
Mariette Carels ◽  
David Shepherd
Keyword(s):  
Ammonium Nitrate ◽  
Yeast Extract ◽  
Organic Nitrogen ◽  
Nitrogen Nutrition ◽  
Nitrogen Sources ◽  
Pigment Production ◽  
The Other ◽  
Chemical Transformations ◽  
Pigment Formation ◽  
Cultural Conditions

Thirty-nine strains from the genus Monascus were cultivated aerobically to study the relation between nitrogen nutrition and sporulation and pigment production. The effects of yeast extract, nitrate, ammonium, and ammonium nitrate have been compared. During cultivation the pHs of the different media are not the same, resulting in the formation of different coloured pigments. When the source of nitrogen is yeast extract or nitrate the pH is around 6.5 and red pigments are formed, whereas with ammonium or ammonium nitrate the pH is around 2.5 and the pigments are orange. It is proposed that only the orange pigments, monascorubrin and rubropunctatin, are produced biosynthetically and that the other pigments are formed from these by chemical transformations depending on the cultural conditions. The presence of organic nitrogen is optimal for growth and unfavourable for pigment production. Reduced growth and best pigment formation occurs with the three other nitrogen sources. Nitrate stimulates conidiation and sexual reproduction, while ammonium is inhibitory. Pigment production is better when conidiation is reduced. A mechanism is proposed for the control of sporulation and pigment production.

scholarly journals Nitrogen Sources for Tomato and Pepper Production

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 518C-518
Author(s):  
Elizabeth T. Maynard
Keyword(s):  
Ammonium Nitrate ◽  
Total Yield ◽  
Fruit Size ◽  
Calcium Nitrate ◽  
Nitrogen Sources ◽  
Chicken Manure ◽  
Blossom End Rot ◽  
Urea Ammonium Nitrate ◽  
Black Plastic ◽  
Nitrogen Treatments

Three nitrogen sources applied through drip irrigation were compared to preplant-applied urea to evaluate their effects on tomato (Lycopersicon esculentum Mill.) and bell pepper (Capsicum annum L.) earliness, yield, and blossom end rot (BER) in 1995 and 1996. Calcium nitrate (CaNO3), urea ammonium nitrate (UAN), and ammonium nitrate (NH4NO3) were applied at 11.2 kg N/ha weekly beginning 2 weeks after transplanting for a total of 8 weeks. The urea treatment received 112 kg N/ha before planting and fertigated treatments received 22.4 kg N/ha from urea before planting. In 1995 only, two additional treatments were fertilized with chicken manure only (1.3N–0.7P–0.8K) at 112 kg N/ha and 168 kg N/ha. In 1996, nitrogen treatments were compared at two levels of potassium fertilization: 0 or 269 kg K/ha. `Sunrise' or `Mountain Spring' tomatoes and `Ranger' peppers were transplanted into black plastic in mid to late June each year. Nitrogen treatments had no effect on marketable or total yield, fruit size, or BER of tomatoes. Total pepper yield was lower with urea than with CaNO3; early and marketable yields showed similar trends, but differences were not consistently significant. UAN and NH4NO3 pepper yields were usually similar to yield with CaNO3, but did not always differ from urea yields. Compost treatments produced yields intermediate between urea and fertigated treatments in 1995. In 1996, peppers from UAN and NH4NO3 plots had more BER (0.5% to 1%) than CaNO3 plots (0%); urea plots had an intermediate amount of BER (0.2%).

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