scholarly journals  Comparison between thermal hydrolysis and enzymatic proteolysis processes for the preparation of tilapia skin collagen hydrolysates

2013 ◽  
Vol 31 (No. 1) ◽  
pp. 1-4 ◽  
Author(s):  
w. Wang ◽  
Z. Li ◽  
J.-Z. Liu ◽  
Y.-J. Wang ◽  
S.-H. Liu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Radical Scavenging Activity ◽  
Average Molecular Weight ◽  
Radical Scavenging ◽  
Thermal Hydrolysis ◽  
Hydrolysis Process ◽  
Enzymatic Proteolysis ◽  
Skin Collagen ◽  
Activity Yield ◽  
Collagen Hydrolysates

The tilapia (Oreochromis niloticus) skin hydrolysate was produced by thermal or enzymatic hydrolysis processes. Several product characteristics were studied such as the average molecular weight, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, yield, and protein content, in order to compare thermal hydrolysis and enzymatic proteolysis processes for the hydrolysed tilapia skin collagen production. The effects of the following hydrolysis parameters (retorting time and pH, protease combination, and proteolysis time) were studied. Compared with the thermal hydrolysis process, the enzymatic proteolysis process needed less time and milder conditions, under which hydrolysates could be obtained as low molecular weight antioxidant peptides.

scholarly journals FUNCTIONAL PROPERTIES OF CATFISH SKIN COLLAGEN HYDROLYSATES

2021 ◽  
Vol 52 (6) ◽  
pp. 1528-1540
Author(s):  
A. A. Ayat ◽  
K. A. Shakir
Keyword(s):  
Functional Properties ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Consumption ◽  
Degree Of Hydrolysis ◽  
Foaming Properties ◽  
Soluble Collagen ◽  
Skin Collagen ◽  
Collagen Hydrolysates

Collagen hydrolysates were obtained from catfish skin collagen hydrolysis using catfish collagenase, pepsin and trypsin individually and mixed for 15-300min. The degree of hydrolysis , antioxidant activity based on, DPPH radical-scavenging activity (RSA), and reducing power (RP) for all obtained hydro lysates were studied , then the collagenase hydrolysate was (CH)  was selected to be evaluated for  antibacterial activity , functional properties including solubility, emulsification and foaming  properties besides the toxicity  . The highest values for RSA (72.5%) has been noticed when DH reached (24.30%) after 30 min. of hydrolysis by collagenase (CH). The water and oil holding capacities for this hydro lysate was compared to that for ASC (acid soluble collagen and PSC (pepsin soluble collagen), the results showed that the values recorded by CH were significantly higher than ASC & PSC. The molecular weight of CH peptides ranged from 180 to 11 Da. as analyzed by SDS- PAGE.  The toxicity assay result revealed that CH is safe for human consumption. There was no antibacterial function for CH toward E.coli and S. aureus. At concentration of 2mg/ml. This study suggests that catfish collagen hydro lysate could be a good natural alternative for synthetic antioxidants in food industries.   

scholarly journals Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

2020 ◽  
Vol 15 (3) ◽  
pp. 119
Author(s):  
Ir. Yusro Nuri Fawzya, MSi ◽  
Nugrah Analiadi Putra ◽  
Arif Budi Witarto ◽  
Gintung Patantis
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Sea Cucumber ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Degree Of Hydrolysis ◽  
Dpph Method ◽  
Collagen Hydrolysates ◽  
Stichopus Horrens

Golden sea cucumber or locally known as “teripang emas” is one of Indonesia’s most popular sea cucumber and widely processed as functional food or supplement due to its bioactivities. The sea cucumber is often misidentified due to its morphological similarities with other Stichopus spp. This study aimed to identify the golden sea cucumber obtained from West Nusa Tenggara, Indonesia, by a molecular method and study the antioxidant activities of its collagen hydrolysates. The hydrolysates were produced by hydrolyzing acid collagen extract using neutrase for 30, 60, 120, 180, and 240 mins. The products were then analyzed for their degree of hydrolysis, peptide content, molecular weight distribution and radical scavenging activity by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. Results showed that hydrolysis for 180 mins was optimal in producing the highest peptide content, 12.79 ± 0.44 mg/mL, with a degree of hydrolysis (DH) of 55.2 ± 1.50%. However, the highest antioxidant activity (IC50 of 5.25 ± 0.15 mg/mL) was demonstrated after 60 mins hydrolysis with molecular weight (MW) ranged from less than 14.4 kDa to approximately 25 kDa. The hydrolysate might be categorized as a weak to moderate antioxidant. Based on the molecular identification, the golden sea cucumber had 99% similarities with Stichopus horrens and S. monotuberculatus. 

scholarly journals Radical scavenging activity of crude polysaccharides from Camellia sinensis

2011 ◽  
Vol 63 (3) ◽  
pp. 717-721
Author(s):  
Fan Yang ◽  
Zhiwei Yang ◽  
Jianbo Xiao
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Camellia Sinensis ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Molecular Weights

A preparation of crude polysaccharides (TPS) was isolated from Camellia sinensis by precipitation and ultrafiltration. TPS1, TPS2, and TPS3 had molecular weights of 240, 21.4, and 2.46 kDa, respectively. The radical scavenging activities of TPS were evaluated by DPPH free radical, hydroxyl radical and superoxide radical scavenging. These results revealed that TPS exhibited strong radical scavenging activity in a concentration-dependent manner. TPS3 with lowest molecular weight showed a higher radical scavenging activity.

scholarly journals Purification, Characterization, Prebiotic Preparations and Antioxidant Activity of Oligosaccharides from Mulberries

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2329 ◽  
Author(s):  
Erna Li ◽  
Shiyuan Yang ◽  
Yuxiao Zou ◽  
Weiwei Cheng ◽  
Bing Li ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Average Molecular Weight ◽  
Radical Scavenging ◽  
Lactobacillus Rhamnosus ◽  
Gel Permeation Chromatography ◽  
Water Soluble ◽  
Gas Chromatography Mass Spectrometry ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Power

A water-soluble oligosaccharide termed EMOS-1a was prepared by enzymatic hydrolysis of polysaccharides purified from mulberries by column chromatography. The chemical structure of the purified fraction was investigated by ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography–mass spectrometry, which indicated that galactose was the main constituent of EMOS-1a. Chemical analyses showed that the uronic acid and sulfate content of EMOS-1a were 5.6% and 8.35%, respectively, while gel permeation chromatography showed that EMOS-1a had an average molecular weight of 987 Da. The antioxidant activities of EMOS-1a were next investigated, and EMOS-1a exhibited concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, Trolox equivalent antioxidant capacity, and ferric reducing antioxidant power. The level of proliferation of Lactobacillus rhamnosus reached 1420 ± 16% when 4% (w/v) EMOS-1a was added, where the number of colonies in MRS (de Man, Rogosa, and Sharpe) medium with no added oligosaccharide was defined as 100% proliferation. These results indicate that the oligosaccharide EMOS-1a could be used as a natural antioxidant in prebiotic preparations.

scholarly journals Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5337 ◽  
Author(s):  
Chanikan Sonklin ◽  
Natta Laohakunjit ◽  
Orapin Kerdchoechuen
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Peptide Chain ◽  
Antioxidant Power ◽  
Meal Protein ◽  
Ferric Reducing Antioxidant Power

Background Bioactive peptides can prevent damage associated with oxidative stress in humans when consumed regularly. Recently, peptides have attracted immense interest because of their beneficial functional properties, safety and little or no side effects when used at high concentration. Most antioxidant peptides are small in size, less than 1 kDa, and contains a high proportion of hydrophobic amino acid. Particularly, tyrosine, leucine, alanine, isoleucine, valine, lysine, phenyalanine, cysteine, methionine and histidine in peptide chain exhibited high antioxidant activity. Mungbean meal protein (MMP) is highly abundant in hydrophobic amino acids. It indicated that MMP might be a good source of antioxidants. Therefore, the objectives were to optimize the conditions used to generate mungbean meal protein hydrolysate (MMPH) with antioxidant activity from bromelain and to investigate the antioxidant activities of different molecular weight (MW) peptide fraction. Methods Response Surface Methodology (RSM) was used for screening of the optimal conditions to produce MMPH. After that MMPH was fractionated using ultrafiltration membranes with different MW distributions. Crude-MMPH and four fractions were investigated for five antioxidant activities: 2,2,1-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, superoxide, ferric reducing antioxidant power (FRAP) and metal ion chelation activity. Results The optimal condition to produce the MMPH was 15% (w/w) of bromelain and hydrolysis time for 12 h which showed the greatest DPPH and ABTS radical scavenging activity. After mungbean protein from optimal condition was separated based on different molecular weight, the DPPH radical scavenging activity was the highest for the F4 (less than 1 kDa) peptide fraction. Metal ion chelating activity was generally weak, except for the F4 that had a value of 43.94% at a protein concentration of 5 mg/mL. The F4 also exhibited high hydroxyl and superoxide activities (54 and 65.1%), but moderate activity for ferric reducing antioxidant power (0.102 mmole Fe2+/g protein) compared to other peptide fractions and crude-MMPH. Molecular weight and amino acid were the main factors that determined the antioxidant activities of these peptide fractions. Results indicated that F4 had strong antioxidant potentials. Discussion The lowest MW fraction (less than 1 kDa) contributed to the highest DPPH, superoxide, hydroxyl and metal chelation activity because influence of low MW and high content of hydrophobic amino acid in peptide chain. Results from this study indicated that MMPH peptides donate protons to free radicals because they had significantly high DPPH value compared to superoxide, hydroxyl and FRAP, which reactions were electron donation. Moreover, MMPH peptides had the ability to inhibit transition metal ions because of highly abundant glutamic acid and aspartic acid in peptide chain.

scholarly journals Chitooligosaccharides from squid pen prepared using different enzymes: characteristics and the effect on quality of surimi gel during refrigerated storage

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Avtar Singh ◽  
Soottawat Benjakul ◽  
Thummanoon Prodpran
Keyword(s):  
Microbial Growth ◽  
Water Solubility ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Radical Scavenging Activity ◽  
Average Molecular Weight ◽  
Radical Scavenging ◽  
Refrigerated Storage ◽  
Surimi Gel

Abstract Chitooligosaccharides (COS) from squid pen produced using amylase, lipase and pepsin were characterized. COS produced by 8% (w/w) lipase (COS-L) showed the maximum FRAP and ABTS radical scavenging activity than those prepared using other two enzymes. COS-L had the average molecular weight (MW) of 79 kDa, intrinsic viscosity of 0.41 dL/g and water solubility of 49%. DPPH, ABTS radical scavenging activities, FRAP and ORAC of COS-L were 5.68, 322.68, 5.66 and 42.20 μmol TE/g sample, respectively. Metal chelating activity was 2.58 μmol EE/g sample. For antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COS-L against the targeted bacteria were in the range of 0.31–4.91 mg/mL and 0.62–4.91 mg/mL, respectively. Sardine surimi gel added with 1% (w/w) COS-L showed the lower PV, TBARS and microbial growth during 10 days of storage at 4 °C. COS-L from squid pen could inhibit lipid oxidation and extend the shelf-life of refrigerated sardine surimi gel. Graphical abstract

The Radical Scavenging Activity of Protein Hydrolysates Prepared from Mercenariamercenaria and Ruditapesphilippinarum

2011 ◽  
Vol 361-363 ◽  
pp. 748-752 ◽  
Author(s):  
Jiao Jiao Mu ◽  
Qian Cheng Zhao ◽  
Jian Wei Li
Keyword(s):  
Molecular Weight ◽  
Hydroxyl Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Mercenaria Mercenaria ◽  
Ruditapes Philippinarum ◽  
Protein Hydrolysates ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Enzymatic Methods

The Mercenaria mercenaria protein hydrolysates (MMPH) and Ruditapes philippinarum protein hydrolysates (RPPH) were prepared by three enzymatic methods, and the radical scavenging activity of the protein hydrolysates was analyzed. The results showed that the protein hydrolysates prepared by the combination of Protamex and Flavourzyme exhibited significant radical scavenging activity; For DPPH radical, the EC50values of MMPH and RPPH were 33.0 mg/mL and 93.0 mg/mL, respectively, and for hydroxyl radical, the EC50values of the protein hydrolysates were 7.5 mg/mL and 7.0 mg/mL, respectively; The protein hydrolysates were further fractionated by ultrafiltration membrane of 6000 Da, and the radical scavenging activity of the resultant fraction with lower molecular weight (<6000 Da) was significantly (P<0.05) increased, and for DPPH radical, the EC50values of MMPH and RPPH fractions were 29.5 mg/mL and 58.5 mg/mL, respectively, and for hydroxyl radical, the EC50 values of the fractions were 6.0 mg/mL and 7.0 mg/mL, respectively.

scholarly journals Ultrasound-Assisted Ultra-Mild-Acid Hydrolisis of -Carrageenan

REAKTOR ◽  
2018 ◽  
Vol 17 (4) ◽  
pp. 191
Author(s):  
Ratnawati Ratnawati ◽  
Nita Indriyani
Keyword(s):  
Molecular Weight ◽  
Acid Hydrolysis ◽  
Average Molecular Weight ◽  
Weight Fraction ◽  
Number Average Molecular Weight ◽  
Hydrolysis Process ◽  
Higher Temperature ◽  
Ultrasound Assisted ◽  
Hydrolysis Of ◽  
Mild Acid

The low molecular weight fraction of κ-carrageenan is useful in biomedical applications. An ultrasound-assisted acid hydrolysis of κ-carrageenan has been studied. κ-carrageenan with an initial number-average molecular weight of 629 kDa was dispersed in distilled water to form a 5 g/l solution. The pH (3 and 6) of the solution was adjusted by adding HCl solution. The depolymerization reaction was carried out in an ultrasonic device at various temperatures (30, 40, 50, and 60°C) and times (8, 16, 24, and 32 min). The experimental results showed that ultrasound positively contributed to acid hydrolysis process. The number-average molecular weight of the treated k-carrageenan was lower or the percentage of reduction was higher at lower pH, longer reaction time, and higher temperature. The lowest number-average molecular weight (14 kDa) or the highest percent of molecular weight reduction reduction (97.7%) was achieved after ultrasonic irradiation at 60°C and pH 3 for 32 min. Keywords: depolymerization; midpoint scission; ultrasonication

scholarly journals Antioxidant Activity of Protein Hydrolysates from Aqueous Extract of Velvet Antler (Cervus elaphus) as Influenced by Molecular Weight and Enzymes

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Lei Zhao ◽  
Yang-Chao Luo ◽  
Cheng-Tao Wang ◽  
Bao-Ping Ji
Keyword(s):  
Molecular Weight ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Velvet Antler ◽  
Dpph Radical Scavenging ◽  
Peptide Fractions

The crude protein hydrolysates from aqueous extract of velvet antler (AEVA) were prepared by simulated gastrointestinal digestion (SGI, pepsin-pancreatin) using pancreatin-pepsin, alcalase and neutrase. The resulting hydrolysates were separated by sequential ultrafiltration into four fractions. The antioxidant activities of peptide fractions were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and Fe2+-chelating assays. Results showed that the hydrolysate prepared by SGI had a low degree of hydrolysis, which was significantly improved with altered proteases, such as pancreatin-pepsin and alcalase. Antioxidant activities of peptide fractions varied with molecular weight (MW) and the enzyme used. Generally, low-MW peptide fractions had higher ABTS radical scavenging activity and Fe2+-chelating ability, and high-MW peptide fractions were more effective in DPPH radical scavenging activity and reducing power.

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