scholarly journals Evaluation of the contraceptive effects of carprofen, flunixin meglumine and meloxicam in rats

2017 ◽  
Vol 62 (No. 5) ◽  
pp. 274-278
Author(s):  
Z. Paksoy ◽  
A. Kirbas
Keyword(s):  
Single Dose ◽  
Implantation Rate ◽  
Control Group ◽  
Double Dose ◽  
Sprague Dawley ◽  
Dose Administration ◽  
Treatment Groups ◽  
Flunixin Meglumine ◽  
Sprague Dawley Rats ◽  
Implantation Sites

The objective of this study was to determine the suitability of carprofen, flunixin meglumine and meloxicam for use in emergency contraception. Forty-eight pregnant Sprague-Dawley rats were used as material. Five groups were subjected to treatments while one group served as a control. The numbers of animals in each group were equal (n = 8). Treatment groups were administered carprofen (10 mg/kg, single or double dose, s.c.), flunixin meglumine (5 mg/kg, single or double dose, i.m.) and meloxicam (2 mg/kg, a single dose, s.c.) on the third day after mating. The control group received saline. The rats were sacrificed on Day 7 of gestation. Luteal spots and implantation sites were recorded. Pre-implantation loss was calculated by subtracting the number of luteal spots from the number of implantation sites. Compared with the control, the administration of flunixin meglumine (double dose), carprofen (double dose) and meloxicam highly significantly decreased the implantation rate (P < 0.001). Single dose administration of flunixin meglumine and carprofen led to significant decreases (P < 0.01). In conclusion, this study indicates that carprofen, flunixin meglumine and meloxicam treatment cause a decline in implantation rate in rats.

scholarly journals Implantation and pregnancy outcome of Sprague-Dawley rats exposed to pirimiphos-methyl

2019 ◽  
Vol 53 (3) ◽  
pp. 139-145
Author(s):  
Tolulope Oyesola ◽  
Bolanle Iranloye ◽  
Olufeyi Adegoke
Keyword(s):  
Implantation Rate ◽  
Serum Levels ◽  
Control Group ◽  
Sprague Dawley ◽  
Uterine Receptivity ◽  
Pregnant Rats ◽  
Sprague Dawley Rats ◽  
Pirimiphos Methyl ◽  
Implantation Sites ◽  
Access To Food

AbstractObjective. This study was designed to investigate the effect of sublethal doses (10, 60, and 120 mg/kg of pirimiphos-methyl on implantation and pregnancy in female Sprague-Dawley rats. Pirimiphos-methyl is a pesticide widely used worldwide, especially in Africa to protect food against pests and has gained widespread acceptance.Methods. Pregnant Sprague-Dawley rats used for this study had access to food and water ad libitum and were divided into a control group and three experimental groups based on dose of chemical given. The pregnant rats were given pirimiphos-methyl orally on days 1–5, 1–7, 7–18th day of gestation and from day 1 to term. Implantation studies were carried out on days 6 and 8 of pregnancy, while the fetal parameters were ascertained on day 19 of pregnancy and at term. Serum levels of progesterone and estradiol were measured on days 6, 8 and 19 of pregnancy.Results. Sublethal administration of pirimiphos-methyl showed decreased number of implantation sites on days 6 and 8, fetal weight, crown-to-rump length, length of umbilical cord and placenta weight (day 19), birth weight, litter size and total number (at term) in rats administered with pirimiphos-methyl when compared with control.Conclusion. Administration of pirimiphos-methyl resulted in a reduced implantation rate due to decreased uterine receptivity caused by an imbalance in the level of estradiol and progesterone and impaired reproductive outcome during pregnancy.

scholarly journals Pharmacokinetics Interaction between Imatinib and Genistein in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Zhe Wang ◽  
Li Wang ◽  
Meng-ming Xia ◽  
Wei Sun ◽  
Cheng-ke Huang ◽  
...  
Keyword(s):  
Single Dose ◽  
Multiple Dose ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Group Control Group

The objective of this work was to investigate the effect of orally administered genistein on the pharmacokinetics of imatinib and N-desmethyl imatinib in rats. Twenty-five healthy male SD (Sprague-Dawley) rats were randomly divided into five groups: A group (control group), B group (multiple dose of 100 mg/kg genistein for consecutive 15 days), C group (multiple dose of 50 mg/kg genistein for consecutive 15 days), D group (a single dose of 100 mg/kg genistein), and E group (a single dose of 50 mg/kg genistein). A single dose of imatinib is administered orally 30 min after administration of genistein (100 mg/kg or 50 mg/kg). The pharmacokinetic parameters of imatinib and N-desmethyl imatinib were calculated by DAS 3.0 software. The multiple dose of 100 mg/kg or 50 mg/kg genistein significantly (P<0.05) decreased theAUC0-tandCmaxof imatinib.AUC0-tand theCmaxof N-desmethyl imatinib were also increased, but without any significant difference. However, the single dose of 100 mg/kg or 50 mg/kg genistein has no effect on the pharmacokinetics of imatinib and N-desmethyl imatinib. Those results indicated that multiple dose of genistein (100 mg/kg or 50 mg/kg) induces the metabolism of imatinib, while single dose of genistein has no effect.

scholarly journals The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses

2017 ◽  
Vol 2017 ◽  
pp. 1-6
Author(s):  
Malene Nerstrøm ◽  
Peter-Martin Krarup ◽  
Lars Nannestad Jorgensen ◽  
Magnus S. Ågren
Keyword(s):  
Tissue Repair ◽  
Single Layer ◽  
Control Group ◽  
Sprague Dawley ◽  
Colonic Anastomoses ◽  
Treatment Groups ◽  
Sprague Dawley Rats ◽  
Heparan Sulfates ◽  
Biomechanical Strength ◽  
Mimetic Compound

Background. The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. Methods. We evaluated the following two treatment groups of male Sprague Dawley rats (220–256 g): control-treated colonic anastomoses (n=25) and OTR4120-treated colonic anastomoses (n=25). We resected 10 mm of the left colon and then applied either saline alone (control) or OTR4120 (100 μg/mL) in saline to the colonic ends before an end-to-end single-layer anastomosis was constructed and again on the anastomosis before the abdomen and skin were closed. Results. On postoperative day 3, the anastomotic breaking strengths were 1.47 ± 0.32 N (mean ± SD) in the control group and 1.52 ± 0.27 N in the OTR4120-treated animals (P=0.622). We also found that the hydroxyproline concentration (indicator of collagen) in the anastomotic wounds did not differ (P=0.571) between the two groups. Conclusions. Our data demonstrate that a single local application of OTR4120 intraoperatively did not increase the biomechanical strength of colonic anastomoses at the critical postoperative day 3 when the anastomoses are the weakest.

scholarly journals Feeding Sprague Dawley Rats With Jordanian Wild Edible Plants and a High Fat Diet Reduced the Malondialdehyde Levels

2019 ◽  
Vol 11 (10) ◽  
pp. 71
Author(s):  
Anwar A. Al-Assaff ◽  
Hamed R. Takruri
Keyword(s):  
High Fat Diet ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
The Other ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat ◽  
Treatment Groups ◽  
Sprague Dawley Rats ◽  
Plant Powders

The objective of this study was to determine the effect of selected Jordanian wild edible plant on lipid peroxidation and lipid profile in adult male Sprague Dawley rats fed high-fat diet. Fiftysix male, adult Sprague-Dawley rats at eight weeks of age, weighing about 200g were distributed into 7 experimental groups, 7 rats each . The groups included a negative control group that was fed a normal fat diet (NFD) and a possitve control group that was fed a high fat diet (HFD) (45% calories from fat). The six treatment groups were fed a HFD for the first 4 weeks of the experiment and a HFD with 9% of one of the selected dried plants for another 4 weeks. The treatment groups are sumac, thyme, clary, gundelia, garden rocket and wild mint. Blood samples were collected from the right heart ventricle. Serum malondialdehyde, lipid profile and fasting blood glucose were measured for rats. Results showed that the addition of different dried plant powders to the HFD did not significantly affect serum levels of TG, TC, HDL, LDL and fasting blood glucose. On the other hand, malondialdehyde (MDA) levels were significantly (p &lt; 0.05) higher in the HFD group (4.09&plusmn;0.45 mmol/ml) than those of other groups. MDA serum levels for the other groups were as follows: NFD (2.47&plusmn;0.05), sumac (2.45&plusmn;0.13), thyme (2.88&plusmn;0.07), clary (2.97&plusmn;0.16), garden rocket (2.96&plusmn;0.11), gundelia (2.92&plusmn;0.16) and wild mint (2.68&plusmn;0.09). These levels were not sinificantly different from each other. It is concluded that incorporating dried plant powders in rat diets had a significantly positive effect only on lipid peroxidation assay as indicated by serum MDA levels.

scholarly journals Androgenic Effects of Cinnamomum camphora in Male Sprague-dawley Rats

2019 ◽  
pp. 1-13
Author(s):  
O. H. Ayoade ◽  
G. G. Akunna ◽  
F. I. Duru
Keyword(s):  
Dose Group ◽  
Low Dose ◽  
Activity Level ◽  
Control Group ◽  
High Dose ◽  
Activity Levels ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Sprague Dawley Rats ◽  
Medium Dose

This study evaluated camphora-induced androgenic and histopathological changes in male Sprague-Dawley rats. Thirty-five animals weighing 200 g±20 g were used for this study and randomly divided equally into five groups, with seven rats in each group. Group A animals (normal control group) were served water and rat chow only; Groups B-D (treatment groups) were orally administered camphora in doses of 1 g/kg (Low-dose), 2 g/kg (Medium-dose) and 4 g/kg (High-dose) respectively while Group E (vehicle group) were orally administered 6 mL/kg olive oil (a solvent for camphora) per day for 56 days. There was a significant decrease (P< .05) in activity levels of Follicle-Stimulating Hormone (FSH); Superoxide Dismutase (SOD) when the treatment was compared with the control group. Also, a significant decrease (P< .05) in activity level of FSH was observed when the Medium-dose group was compared with Low-dose group. Insignificant irregular pattern in activity level of Testosterone was observed across the treatment groups when compared with the control. However, a significant increase (P< .05) in activity level of Testosterone was observed when the High-dose group was compared with the Medium-dose group. There was a significant increase (P< .05) in activity levels of Luteinizing Hormone (LH) and Malondialdehyde (MDA) when the treatment was compared with the control group. Semen analysis showed reduction in sperm concentration, motility and morphology with increasing concentration of camphora. Significant decrease was recorded in testicular weight when High-dose group was compared to Control and Low-dose groups. Histopathological changes were seen in the testes of the camphor administered groups, ranging from mild disintegrated interstitial tissues in Low-dose to severe degeneration and disintegration of both seminiferous and interstitial tissues in the testes in the Medium-dose and High-dose groups. In conclusion, camphora had androgenic and toxic effects on testis and may cause testicular tissue damage.

scholarly journals Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α

2014 ◽  
Vol 23 (3) ◽  
pp. 133-8 ◽  
Author(s):  
Sri W.A. Jusman ◽  
Febriana C. Iswanti ◽  
Franciscus D. Suyatna ◽  
Frans Ferdinal ◽  
Septelia I. Wanandi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normobaric Hypoxia ◽  
Control Group ◽  
Strongly Correlated ◽  
Sprague Dawley ◽  
Oxidative Stress Parameter ◽  
Treatment Groups ◽  
Stress Parameter ◽  
Sprague Dawley Rats ◽  
Mrna And Protein Expression

Background: Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.Methods: This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O2: 90% N2) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.Results: Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.Conclusion: Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.

scholarly journals Safety Study of Single-Dose Intravenously Administered DOTA-Siglec-9 Peptide in Sprague Dawley Rats

2019 ◽  
Vol 38 (1) ◽  
pp. 4-11
Author(s):  
Paulina Chrusciel ◽  
Emrah Yatkin ◽  
Xiang-Guo Li ◽  
Ulla-Marjut Jaakkola ◽  
Juhani Knuuti ◽  
...  
Keyword(s):  
Single Dose ◽  
European Medicines Agency ◽  
Control Group ◽  
Sprague Dawley ◽  
Clinical Dose ◽  
Technical Requirements ◽  
Positron Emission ◽  
Sprague Dawley Rats ◽  
Toxicological Studies ◽  
Vascular Adhesion

The peptide-based radioactive compound [68Ga]Ga-DOTA-Siglec-9 is a novel agent for imaging of inflammation with positron emission tomography. The drug target of [68Ga]Ga-DOTA-Siglec-9 is vascular adhesion protein 1. Previous studies have obtained promising results with [68Ga]Ga-DOTA-Siglec-9 in experimental animals. However, before taking this novel imaging agent into clinical trials, safety and toxicological studies need to be performed with the nonradioactive precursor compound DOTA-Siglec-9. This extended single-dose toxicity study was designed to provide information on the major toxic effects of DOTA-Siglec-9 and to indicate possible target organs after a single intravenous (iv) injection in rats. The study was performed using 60 adult Hsd: Sprague Dawley rats and included a control group and a treatment group to investigate the toxicity of DOTA-Siglec-9 solution at a final concentration of 0.2 mg/mL after a single iv injection of 582 µg/kg. The maximum dose tested was 1,000-fold the clinical dose on a mg/kg basis as indicated in European Medicines Agency International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use guideline M3(R2). The planned human clinical dose is approximately 0.582 µg of DOTA-Siglec-9 per kg of body mass. This study demonstrates that iv administration of DOTA-Siglec-9 at a dose of 582 µg/kg was well tolerated in rats and did not produce toxicologically significant adverse effects.

scholarly journals The Effect of Myrmecodia pendans Ethanol Extract on Inflamed Pulp: Study on Sprague Dawley Rats

2019 ◽  
Vol 3 (2) ◽  
pp. 115
Author(s):  
Janti Sudiono ◽  
Meylisa Hardina
Keyword(s):  
Treatment Group ◽  
Healing Process ◽  
Ethanol Extract ◽  
Flavonoid Compound ◽  
Control Group ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Group Ii

Background: Inflammation is a body response caused by injury and infection. Pulpitis is a pulp tissue inflammation which is the continuous process of pulp hyperemia by bacteria invasion. Myrmecodia pendans or Sarang semut is known to contain flavonoid compound which has the anti inflammation effect. The purpose of this study is to investigate the effect of Myrmecodia pendans ethanol extract on the healing process of pulp inflammation.Materials and Methods: This experimental study involved pre- and post-in vivo treatment of 27 Sprague Dawley rats in which the inducted pulpitis model was obtained by injecting 0.01 mL Porphyromonas gingivalis into the dental pulp for 48 hours. Subjects were divided randomly into Group I (negative control), Group II (pulpitis treated by Myrmecodia pendans extract ethanol as treatment group), and Group III (pulpitis treated by Ca(OH)2 as positive control group). Group II and III as pulpitis treatment groups were divided into subgroups based on the induction periods of 48 hours (2 days), 168 hours (7 days), and 366 hours (14 days). All specimens were processed into the slides and evaluated microscopically for the healing process.Results: The result of this study showed significant difference (p<0.05) among groups on day 2, 4 and 7. On day 4, the pulpitis treatment group of Myrmecodia pendans extract showed better healing process than Ca(OH)2. On day 7, the pulpitis treatment group of Ca(OH)2 showed better healing process than Myrmecodia pendans extract. On day 14, both of the pulpitis treatment groups showed normal pulp.Conclusion: Myrmecodia pendans ethanol extract is effective for the healing process of inflamed pulp.Keywords: inflamed pulp, Myrmecodia pendans, sarang semut, Ca(OH)2 , healing process

Efficacy of Perioperative Thromboprophylactic Agents in the Maintenance of Anastamotic Patency and Survival of Rat Microvascular Free Groin Flaps

2003 ◽  
Vol 129 (3) ◽  
pp. 176-182 ◽  
Author(s):  
Prad Murthy ◽  
Michael V. Riesberg ◽  
Steve Hart ◽  
Andres Bustillo ◽  
Carlos. S. Duque ◽  
...  
Keyword(s):  
Therapy Group ◽  
Combined Therapy ◽  
Control Group ◽  
Low Molecular Weight ◽  
Microvascular Anastomosis ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Sprague Dawley Rats ◽  
Blinded Study ◽  
Arterial Patency

OBJECTIVES: We sought to determine the thromboprophylactic effects of low-molecular-weight heparin (LMWH) and pentoxifylline. DESIGN: Randomized blinded study. MATERIALS AND METHODS: Sixty Sprague-Dawley rats were assigned to 1 of 4 treatment groups: (1) control group (n = 15), (2) pentoxifylline group (n = 15), (3) LMWH group (n = 15), and (4) combined therapy group (n = 15). A groin/abdominal free flap was elevated in each, with division of the artery and microvascular repair The animals received therapy for 4 days preoperatively and 6 days postoperatively. Flaps were monitored visually, and on the seventh postoperative day, the flap was elevated, and the microvascular anastomosis was assessed for patency. RESULTS: There was a statistically significant improvement in arterial patency in both pentoxifylline and LMWH groups compared with controls ( P < 0.01) but no significant improvement with combined therapy ( P > 0.05). CONCLUSIONS: In this animal model, LMWH and pentoxifylline have significant thrombroprophylactic effects when used individually but not in combination.

scholarly journals Skeletal Muscle Metabolomic Responses to Endurance and Resistance Training in Rats under Chronic Unpredictable Mild Stress

2021 ◽  
Vol 18 (4) ◽  
pp. 1645
Author(s):  
Xiangyu Liu ◽  
Xiong Xue ◽  
Junsheng Tian ◽  
Xuemei Qin ◽  
Shi Zhou ◽  
...  
Keyword(s):  
Resistance Exercise ◽  
Endurance Exercise ◽  
Field Tests ◽  
Treadmill Running ◽  
Control Group ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Sprague Dawley ◽  
Exercise Interventions ◽  
Sprague Dawley Rats

The objectives of this study were to compare the antidepressant effects between endurance and resistance exercise for optimizing interventions and examine the metabolomic changes in different types of skeletal muscles in response to the exercise, using a rat model of chronic unpredictable mild stress (CUMS)-induced depression. There were 32 male Sprague-Dawley rats randomly divided into a control group (C) and 3 experimental groups: CUMS control (D), endurance exercise (E), and resistance exercise (R). Group E underwent 30 min treadmill running, and group R performed 8 rounds of ladder climbing, 5 sessions per week for 4 weeks. Body weight, sucrose preference, and open field tests were performed pre and post the intervention period for changes in depressant symptoms, and the gastrocnemius and soleus muscles were sampled after the intervention for metabolomic analysis using the 1H-NMR technique. The results showed that both types of exercise effectively improved the depression-like symptoms, and the endurance exercise appeared to have a better effect. The levels of 10 metabolites from the gastrocnemius and 13 metabolites from the soleus of group D were found to be significantly different from that of group C, and both types of exercise had a callback effect on these metabolites, indicating that a number of metabolic pathways were involved in the depression and responded to the exercise interventions.

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