scholarly journals Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α

2014 ◽  
Vol 23 (3) ◽  
pp. 133-8 ◽  
Author(s):  
Sri W.A. Jusman ◽  
Febriana C. Iswanti ◽  
Franciscus D. Suyatna ◽  
Frans Ferdinal ◽  
Septelia I. Wanandi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normobaric Hypoxia ◽  
Control Group ◽  
Strongly Correlated ◽  
Sprague Dawley ◽  
Oxidative Stress Parameter ◽  
Treatment Groups ◽  
Stress Parameter ◽  
Sprague Dawley Rats ◽  
Mrna And Protein Expression

Background: Liver is sensitive against hypoxia and hypoxia will stabilize HIF-1α. At the same time, hypoxia will produce reactive oxygen species (ROS) which can be scavenged by Cygb. The purpose of our study is to know, if normobaric hypoxia can induce Cygb expression and its association with HIF-1α stabilization.Methods: This is an experimental study using 28 male Sprague-Dawley rats, 150-200 g weight. Rats are divided into 7 groups: control group and treatment groups that are kept in hypoxic chamber (10% O2: 90% N2) for 6 hours, 1, 2, 3, 7 and 14 days. All rats are euthanized after treatment and liver tissue are isolated, homogenized and analyzed for oxidative stress parameter, expression of Cygb and HIF-1α.Results: Expression of Cygb mRNA and protein was increased on the day-1 after treatment and reach the maximum expression on the day-2 of hypoxia treatment. But, the expression was decreased after the day-3 and slightly increased at the day-14 of hypoxia. The correlation between expression of Cygb and oxidative stress parameter was strongly correlated. Cygb mRNA, as well as protein, showed the same kinetic as the HIF-1, all increased about day-1 and day-2.Conclusion: Systemic chronic hypoxia and/or oxidative stress up-regulated HIF-1α mRNA which is correlated with the Cygb mRNA and protein expression. Cygb mRNA as well as Cygb protein showed the same kinetic as the HIF-1, all increased about day-1 and day-2 suggesting that Cygb could be under the regulation of HIF-1, but could be controlled also by other factor than HIF-1.

scholarly journals Sulodexide Protects Contrast-Induced Nephropathy in Sprague-Dawley Rats

2016 ◽  
Vol 40 (3-4) ◽  
pp. 621-632 ◽  
Author(s):  
Qing Zhao ◽  
Jianyong Yin ◽  
Zeyuan Lu ◽  
Yiwei Kong ◽  
Guangyuan Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caspase 3 ◽  
Vehicle Group ◽  
Control Group ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Contrast Induced Nephropathy ◽  
Sprague Dawley Rats ◽  
Hk2 Cells

Background: Sulodexide is a powerful antithrombin agent with reno-protective property. However, whether it has beneficial effects on Contrast-Induced Nephropathy (CIN) remained elusive. In the current study, we evaluated the therapeutic effects of Sulodexide on CIN and investigated the potential mechanisms. Methods: CIN model was induced by intravenous injection of indomethacin, followed by Ioversol and L-NAME. Sprague-Dawley rats were divided into 4 groups: control group, CIN group, CIN+vehicle group (CIN rats pretreated with vehicle) and CIN+ Sulodexide (CIN rats pretreated with Sulodexide). Sulodexide or an equivalent volume of vehicle was intravenously delivered 30 min before the induction of CIN. All the animals were sacrificed at 24h after CIN and tissues were harvested to evaluate renal injury, kidney oxidative stress and apoptosis levels. Plasma antithrombin III (ATIII) activities were also measured. Results: Compared to the untreated CIN group, improved renal function, reduced tubular injury, decreased levels of oxidative stress and apoptosis were observed in CIN rats receiving Sulodexide injection. In addition, we also found that ATIII activity was significantly higher in Sulodexide-administered group than that in vehicle-injected CIN rats. For in vitro studies, HK2 cells were exposed to Ioversol and the cyto-protective effects of Sulodexide were also determined. Sulodexide pretreatment protected HK2 cells against the cytotoxicity of Ioversol via inhibiting caspase-3 activity. Preincubation with Sulodexide could also attenuate H2O2-induced increases in ROS, apoptosis and caspase-3 levels. Conclusions: Taken together, Sulodexide could protect against CIN through activating ATIII, and inhibiting oxidative stress, inflammation and apoptosis.

Chronotropic, Inotropic and Dromotropic Parameters of the Heart and Oxidative Stress in Rats Receiving High Doses of Fructose

2019 ◽  
Vol 8 ◽  
pp. 1250
Author(s):  
Esmat Radmanesh ◽  
Mahin Dianat ◽  
Narges Atefipour
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
Drinking Water ◽  
Control Group ◽  
Free Access ◽  
Qtc Interval ◽  
Sprague Dawley ◽  
Cvd Risk ◽  
Sprague Dawley Rats ◽  
High Doses

Background: Many risk factors, including nutritional ones, contribute to cardiovascular diseases (CVDs). Increased fructose consumption, for example, can lead to an increase in CVD risk factors, i.e. an increase in blood lipids and the development of insulin resistance. Materials and Methods: In the present study, Sprague Dawley rats were divided into two groups:  control group (free access to tap drinking water for seven weeks), and a group that received fructose 10% in drinking water for seven weeks, (n ═8 per each group). In all groups, before starting the test period and seven weeks after it, electrocardiogram was recorded by Power lab system. Unpaired t-test and two-way ANOVA were used for data analysis. Also, oxidative stress parameters were measured. Results: In the group received high doses of fructose, a significant reduction (P <0.05) was observed in the PR interval (P<0.001) and a significant increase (P<0.05) in the QTc interval. However, there was no significant change in the RR interval and the voltage of the QRS complex. A significant decrease in catalase, superoxide dismutase and glutathione peroxidase (P<0.05) and a significant increase (P<0.05) in malondialdehyde and lactate dehydrogenase were observed in the group that received fructose in comparison with the control group at the end of the experiment. Conclusion: According to our results, the chance of arrhythmias in the rats receiving high doses of fructose was possibly due to the increased oxidative stress in the healthy rats. [GMJ.2019;8:e1250]

scholarly journals Cacao powder supplementation attenuates oxidative stress, cholinergic impairment, and apoptosis in d-galactose-induced aging rat brain

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyoeun Yoo ◽  
Hyun-Sook Kim
Keyword(s):  
Oxidative Stress ◽  
Control Group ◽  
Protective Effects ◽  
Aging Male ◽  
Sprague Dawley ◽  
Advanced Glycation ◽  
Age Related ◽  
Sprague Dawley Rats ◽  
Glycation End Products ◽  
Aging Rat

AbstractAging, a critical risk factor of several diseases, including neurodegenerative disorders, affects an ever-growing number of people. Cacao supplementation has been suggested to improve age-related neuronal deficits. Therefore, this study investigated the protective effects of raw cacao powder on oxidative stress-induced aging. Male Sprague–Dawley rats were divided into 4 groups: Control (C), d-galactose-induced aging (G), d-galactose injection with 10% (LC), and 16% (HC) cacao powder mixed diet. d-galactose (300 mg/3 mL/kg) was intraperitoneally injected into all but the control group for 12 weeks. Cacao supplemented diets were provided for 8 weeks. The levels of serum Malondialdehyde (MDA), Advanced Glycation End-products (AGEs), brain and liver MDA, the indicators of the d-galactose induced oxidative stress were significantly decreased in LC and HC but increased in G. The Acetylcholinesterase (AChE) activity of brain showed that the cholinergic impairment was significantly lower in LC, and HC than G. Furthermore, the expression levels of catalase (CAT), phospho-Akt/Akt, and procaspase-3 were significantly increased in LC and HC. In conclusion, cacao consumption attenuated the effects of oxidative stress, cholinergic impairment and apoptosis, indicating its potential in future clinical studies.

scholarly journals Dietary Supplementation with Chitosan Oligosaccharides Alleviates Oxidative Stress in Rats Challenged with Hydrogen Peroxide

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 55 ◽  
Author(s):  
Ruixia Lan ◽  
Qingqing Chang ◽  
Lilong An ◽  
Zhihui Zhao
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Sprague Dawley ◽  
Antioxidant Power ◽  
Chitosan Oligosaccharides ◽  
Sprague Dawley Rats

Oxidative stress is induced by excessive oxidative radicals, which directly react with biomolecules, and damage lipids, proteins and DNA, leading to cell or organ injury. Supplementation of antioxidants to animals can be an effective way to modulate the antioxidant system. Chitosan oligosaccharides (COS) are the degraded products of chitosan or chitin, which has strong antioxidant, anti-inflammatory, and immune-enhancing competency. Therefore, the current study was conducted to evaluate the hypothesis that dietary supplementation with COS alleviates the damage caused by oxidative stress in Sprague Dawley rats challenged with hydrogen peroxide (H2O2). The rats were randomly divided into three groups: CON, control group, in which rats were fed a basal diet with normal drinking water; AS, H2O2 group, in which rats were fed the basal diet and 0.1% H2O2 in the drinking water; ASC, AS + COS group, in which rats were fed the basal diet with 200 mg/kg COS, and with 0.1% H2O2 in the drinking water. In vitro, COS exhibited better radical scavenging capacity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2−), H2O2, and ferric ion reducing antioxidant power (FRAP) than butylated hydroxy anisole (BHA). In vivo, dietary supplementation with COS alleviated the H2O2-induced oxidative damage, evidenced by comparatively increasing activity of SOD, CAT, GSH-Px, GSH, and T-AOC, and comparatively decreasing level of MDA in serum, liver, spleen, and kidney. COS also comparatively alleviated the H2O2-induced inflammation. In conclusion, COS supplementation reduced lipid peroxidation and restored antioxidant capacity in Sprague Dawley rats, which were challenged with H2O2.

scholarly journals The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses

2017 ◽  
Vol 2017 ◽  
pp. 1-6
Author(s):  
Malene Nerstrøm ◽  
Peter-Martin Krarup ◽  
Lars Nannestad Jorgensen ◽  
Magnus S. Ågren
Keyword(s):  
Tissue Repair ◽  
Single Layer ◽  
Control Group ◽  
Sprague Dawley ◽  
Colonic Anastomoses ◽  
Treatment Groups ◽  
Sprague Dawley Rats ◽  
Heparan Sulfates ◽  
Biomechanical Strength ◽  
Mimetic Compound

Background. The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. Methods. We evaluated the following two treatment groups of male Sprague Dawley rats (220–256 g): control-treated colonic anastomoses (n=25) and OTR4120-treated colonic anastomoses (n=25). We resected 10 mm of the left colon and then applied either saline alone (control) or OTR4120 (100 μg/mL) in saline to the colonic ends before an end-to-end single-layer anastomosis was constructed and again on the anastomosis before the abdomen and skin were closed. Results. On postoperative day 3, the anastomotic breaking strengths were 1.47 ± 0.32 N (mean ± SD) in the control group and 1.52 ± 0.27 N in the OTR4120-treated animals (P=0.622). We also found that the hydroxyproline concentration (indicator of collagen) in the anastomotic wounds did not differ (P=0.571) between the two groups. Conclusions. Our data demonstrate that a single local application of OTR4120 intraoperatively did not increase the biomechanical strength of colonic anastomoses at the critical postoperative day 3 when the anastomoses are the weakest.

scholarly journals The Effects of Honey Administration on Petrol-Induced Oxidative Stress and Hepatotoxicity in Sprague-Dawley Male Rats

2020 ◽  
Vol 1 (1) ◽  
pp. 16-22
Author(s):  
Murtala Bello ABUBAKAR ◽  
Boon Suen ANG
Keyword(s):  
Oxidative Stress ◽  
Pantothenic Acid ◽  
Ambient Air ◽  
Oil Fields ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Oil Refineries ◽  
Sprague Dawley Rats ◽  
Background Exposure

Background: Exposure to petrol is common among individuals working in oil refineries, oil fields, filling stations, automobile mechanical workshops. Exposure to petrol is associated with a number of health hazards including hepatotoxicity and oxidative stress. Honey is rich in many antioxidants such as catalase, flavonoids, thiamine, riboflavin, pyridoxine, pantothenic acid, ascorbic acid and nicotinic acid and was recently reported to reduce petrol-induced hepatotoxicity and nephrotoxicity. Materials and Methods: A total of 32 male Sprague-Dawley rats were randomly divided into four groups: control group (exposed to ambient air daily), petrol exposed group

scholarly journals Evaluation of the contraceptive effects of carprofen, flunixin meglumine and meloxicam in rats

2017 ◽  
Vol 62 (No. 5) ◽  
pp. 274-278
Author(s):  
Z. Paksoy ◽  
A. Kirbas
Keyword(s):  
Single Dose ◽  
Implantation Rate ◽  
Control Group ◽  
Double Dose ◽  
Sprague Dawley ◽  
Dose Administration ◽  
Treatment Groups ◽  
Flunixin Meglumine ◽  
Sprague Dawley Rats ◽  
Implantation Sites

The objective of this study was to determine the suitability of carprofen, flunixin meglumine and meloxicam for use in emergency contraception. Forty-eight pregnant Sprague-Dawley rats were used as material. Five groups were subjected to treatments while one group served as a control. The numbers of animals in each group were equal (n = 8). Treatment groups were administered carprofen (10 mg/kg, single or double dose, s.c.), flunixin meglumine (5 mg/kg, single or double dose, i.m.) and meloxicam (2 mg/kg, a single dose, s.c.) on the third day after mating. The control group received saline. The rats were sacrificed on Day 7 of gestation. Luteal spots and implantation sites were recorded. Pre-implantation loss was calculated by subtracting the number of luteal spots from the number of implantation sites. Compared with the control, the administration of flunixin meglumine (double dose), carprofen (double dose) and meloxicam highly significantly decreased the implantation rate (P &lt; 0.001). Single dose administration of flunixin meglumine and carprofen led to significant decreases (P &lt; 0.01). In conclusion, this study indicates that carprofen, flunixin meglumine and meloxicam treatment cause a decline in implantation rate in rats.

scholarly journals Feeding Sprague Dawley Rats With Jordanian Wild Edible Plants and a High Fat Diet Reduced the Malondialdehyde Levels

2019 ◽  
Vol 11 (10) ◽  
pp. 71
Author(s):  
Anwar A. Al-Assaff ◽  
Hamed R. Takruri
Keyword(s):  
High Fat Diet ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
The Other ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat ◽  
Treatment Groups ◽  
Sprague Dawley Rats ◽  
Plant Powders

The objective of this study was to determine the effect of selected Jordanian wild edible plant on lipid peroxidation and lipid profile in adult male Sprague Dawley rats fed high-fat diet. Fiftysix male, adult Sprague-Dawley rats at eight weeks of age, weighing about 200g were distributed into 7 experimental groups, 7 rats each . The groups included a negative control group that was fed a normal fat diet (NFD) and a possitve control group that was fed a high fat diet (HFD) (45% calories from fat). The six treatment groups were fed a HFD for the first 4 weeks of the experiment and a HFD with 9% of one of the selected dried plants for another 4 weeks. The treatment groups are sumac, thyme, clary, gundelia, garden rocket and wild mint. Blood samples were collected from the right heart ventricle. Serum malondialdehyde, lipid profile and fasting blood glucose were measured for rats. Results showed that the addition of different dried plant powders to the HFD did not significantly affect serum levels of TG, TC, HDL, LDL and fasting blood glucose. On the other hand, malondialdehyde (MDA) levels were significantly (p &lt; 0.05) higher in the HFD group (4.09&plusmn;0.45 mmol/ml) than those of other groups. MDA serum levels for the other groups were as follows: NFD (2.47&plusmn;0.05), sumac (2.45&plusmn;0.13), thyme (2.88&plusmn;0.07), clary (2.97&plusmn;0.16), garden rocket (2.96&plusmn;0.11), gundelia (2.92&plusmn;0.16) and wild mint (2.68&plusmn;0.09). These levels were not sinificantly different from each other. It is concluded that incorporating dried plant powders in rat diets had a significantly positive effect only on lipid peroxidation assay as indicated by serum MDA levels.

scholarly journals Androgenic Effects of Cinnamomum camphora in Male Sprague-dawley Rats

2019 ◽  
pp. 1-13
Author(s):  
O. H. Ayoade ◽  
G. G. Akunna ◽  
F. I. Duru
Keyword(s):  
Dose Group ◽  
Low Dose ◽  
Activity Level ◽  
Control Group ◽  
High Dose ◽  
Activity Levels ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Sprague Dawley Rats ◽  
Medium Dose

This study evaluated camphora-induced androgenic and histopathological changes in male Sprague-Dawley rats. Thirty-five animals weighing 200 g±20 g were used for this study and randomly divided equally into five groups, with seven rats in each group. Group A animals (normal control group) were served water and rat chow only; Groups B-D (treatment groups) were orally administered camphora in doses of 1 g/kg (Low-dose), 2 g/kg (Medium-dose) and 4 g/kg (High-dose) respectively while Group E (vehicle group) were orally administered 6 mL/kg olive oil (a solvent for camphora) per day for 56 days. There was a significant decrease (P< .05) in activity levels of Follicle-Stimulating Hormone (FSH); Superoxide Dismutase (SOD) when the treatment was compared with the control group. Also, a significant decrease (P< .05) in activity level of FSH was observed when the Medium-dose group was compared with Low-dose group. Insignificant irregular pattern in activity level of Testosterone was observed across the treatment groups when compared with the control. However, a significant increase (P< .05) in activity level of Testosterone was observed when the High-dose group was compared with the Medium-dose group. There was a significant increase (P< .05) in activity levels of Luteinizing Hormone (LH) and Malondialdehyde (MDA) when the treatment was compared with the control group. Semen analysis showed reduction in sperm concentration, motility and morphology with increasing concentration of camphora. Significant decrease was recorded in testicular weight when High-dose group was compared to Control and Low-dose groups. Histopathological changes were seen in the testes of the camphor administered groups, ranging from mild disintegrated interstitial tissues in Low-dose to severe degeneration and disintegration of both seminiferous and interstitial tissues in the testes in the Medium-dose and High-dose groups. In conclusion, camphora had androgenic and toxic effects on testis and may cause testicular tissue damage.

scholarly journals Red Guava Juice (Psidium guajava L.) Reduce Oxidative Stress of Toll Gate Collector

2019 ◽  
Vol 39 (4) ◽  
pp. 333
Author(s):  
Riva Mustika Anugrah ◽  
Sugeng Maryanto ◽  
Kusmiyati Tjahjono ◽  
Martha Irene Kartasurya
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Initial Conditions ◽  
Thiobarbituric Acid ◽  
Psidium Guajava ◽  
T Test ◽  
Wilcoxon Test ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Exposure to air pollution can increase the occurrence of oxidative stress. Research showed that guava can reduce oxidative stress in Sprague Dawley rats. This experiment investigated the effect of red guava juice on oxidative stress in toll collectors. The subjects were 20 toll collectors in the treatment group and 20 in the control group. The treatment group received 250 mL red guava juice for 21 days while the controls did not receive anything. Malondialdehyde (MDA) was measured with thiobarbituric acid (TBA) reaction, food consumption was measured by 3×24 hours’ recall. The data were analyzed by paired t-test, Wilcoxon test, independent t-test, Mann Whitney test, and general linear model. The results showed that the effect of red guava juice on the MDA level was significant in the treatment group (p<0.05). A multivariate analysis showed that the effect of red guava juice to MDA level was significant after controlled by age, carbohydrate intake and initial conditions (p<0.05). Red guava juices of 250 mL for 21 days could reduce malondialdehyde (MDA) levels of toll collectors.

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