scholarly journals Detection of Acetyl-CoA Carboxylase (ACCase) Inhibitor Herbicides Resistance in Sterile Wild Oat (Avena sterilis L.) Using Agar Quick Test

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Abdullatief Mohammed Abdurruhman ◽  
Sibel Uygur ◽  
Feyzullah Nezihi Uygur
Keyword(s):  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Avena Sterilis ◽  
Quick Test

ACCase-Inhibiting Herbicide-ResistantAvenaspp. Populations from the Western Australian Grain Belt

2012 ◽  
Vol 26 (1) ◽  
pp. 130-136 ◽  
Author(s):  
M. S. Ahmad-Hamdani ◽  
Mechelle J. Owen ◽  
Qin Yu ◽  
Stephen B. Powles
Keyword(s):  
Herbicide Resistance ◽  
Dose Response ◽  
Resistance Index ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Grain Crops ◽  
High Level ◽  
Western Australian

Avenaspp. are world weeds with many cases of evolved herbicide resistance. In Australia,Avenaspp. (wild oat and sterile oat) are a major problem, especially in grain crops. Acetyl-CoA carboxylase (ACCase)–inhibiting herbicides have been used extensively since the late 1970s forAvenaspp. control. However, continued reliance on these herbicides has resulted in the evolution of resistantAvenaspp. populations. Resistance across many ACCase-inhibiting herbicides was characterized in fourAvenaspp. populations from the Western Australian grain belt. Dose–response experiments were conducted to determine the level of resistance to the aryloxyphenoxypropionates and cyclohexanediones and to the phenylpyrazoline herbicide pinoxaden. On the basis of resistance index values, all four resistant populations exhibited high-level diclofop resistance but varied in the level of resistance to other ACCase-inhibiting herbicides tested. It is evident thatAvenaspp. populations from the Western Australian grain belt have evolved resistance to a number of ACCase-inhibiting herbicides.

Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua)

Weed Science ◽  
1997 ◽  
Vol 45 (6) ◽  
pp. 750-755 ◽  
Author(s):  
Luc Bourgeois ◽  
Norm C. Kenkel ◽  
Ian N. Morrison
Keyword(s):  
Cluster Analysis ◽  
Principal Component ◽  
Petri Dish ◽  
Cross Resistance ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Pairwise Correlation ◽  
Resistance Patterns

The purpose of this study was to determine cross-resistance patterns among wild oat lines resistant to acetyl-CoA carboxylase (ACCase) inhibitors and to determine which, if any, cross-resistant type was more common than another. Discriminatory concentrations of two aryloxyphenoxy-propionates (APP) and three cyclohexanediones (CHD) were determined using a petri-dish bioassay. These concentrations were then applied to 82 resistant wild oat lines identified in previous studies. In addition, two resistant standards (UM1 and UM33) and a susceptible standard (UM5) were included in the experiments. Coleoptile lengths expressed as percentages of untreated controls were used to assess the level of resistance to each herbicide. Large variations were observed among wild oat lines and herbicides. However, cluster analysis summarized the relationship between the five herbicides (variables) and the wild oat lines into three main cross-resistance types. Type A included wild oat lines with high resistance to APP herbicides and no or low resistance to CHD herbicides. Types B and C included those with low to moderate resistant and high levels of resistance to all five herbicides, respectively. Type C was the most common cross-resistance type. Relationships among herbicides were determined using pairwise correlation and principal component analysis (PCA). All correlations were high between APP herbicides and between CHD herbicides but not between APP and CHD herbicides. The first two axes of the PCA accounted for 88.4% of the total variance, with the first axis correlated to the CHD herbicides and the second axis correlated to the APP herbicides. In the PCA, wild oat lines were segregated into the three types identified in the cluster analysis. Although CHD and APP herbicides bind at the same region on the ACCase, resistant wild oat lines respond differently to them.

Competition between ACCase-Inhibitor Resistant and Susceptible Sterile Wild Oat (Avena sterilis) Biotypes

Weed Science ◽  
2013 ◽  
Vol 61 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Ilias S. Travlos
Keyword(s):  
Seed Germination ◽  
Plant Height ◽  
Competitive Ability ◽  
Plant Biomass ◽  
Wild Oat ◽  
Seedling Vigor ◽  
Acetyl Coa Carboxylase ◽  
Replacement Series ◽  
Avena Sterilis ◽  
Canopy Area

Studies were conducted to determine the growth, fecundity, and competitive ability of an acetyl-CoA carboxylase (ACCase)–inhibitor resistant (R) sterile wild oat biotype compared with a susceptible (S) biotype. Seed germination studies indicated that there were no differences in seed germination and seedling vigor between R and S biotypes at any temperature regime. R and S biotypes were grown under noncompetitive and competitive arrangement in the greenhouse. Under noncompetitive greenhouse conditions, growth of the R biotype was similar to that of the S biotype on the basis of plant height, canopy area, and plant biomass. Seed production and weight of R and S plants were also at the same levels. Furthermore, relative competitiveness among the R and S sterile wild oat biotypes was investigated by means of replacement series experiments. The R and S biotypes were compared under seven mixture proportions (6 : 0, 5 : 1, 4 : 2, 3 : 3, 2 : 4, 1 : 5, and 0 : 6). No significant differences in competitive ability were observed between R and S biotypes on the basis of plant height, canopy area, or plant biomass. In most cases, relative crowding coefficient (RCC) values at 20, 60, and 100 d after transplanting (DAT) were close to one, indicating equal competitiveness between the R and S biotypes of wild oat used in this competitive study. However, in some cases, the RCC value was 1.31 for plant height, evident of a slight competitive advantage for the R biotype at 100 DAT. In general, ACCase-inhibitor R and S sterile wild oat biotypes were equally competitive, clearly without any growth penalty for R plants in either noncompetitive or competitive conditions.

Resistant Acetyl-CoA Carboxylase is a Mechanism of Herbicide Resistance in a Biotype of Avena sterilis ssp. ludoviciana

1994 ◽  
Vol 35 (4) ◽  
pp. 627-635 ◽  
Author(s):  
Chanya Maneechote ◽  
Joseph A.M. Holtum ◽  
Christopher Preston ◽  
Stephen B. Powles
Keyword(s):  
Herbicide Resistance ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Avena Sterilis

scholarly journals Fenoxaprop-p-ethyl Susceptibility and Mutation Point Detection of Acetyl-CoA Carboxylase (ACCase) in Different Wild oat (Avena fatua L.) Populations from China

2019 ◽  
Author(s):  
Jun-jie Liu ◽  
Liuyang Lu ◽  
Bai-zhong Zhang ◽  
Xi-ling Chen
Keyword(s):  
Gene Expression ◽  
Critical Role ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Mutation Site ◽  
Multiple Sequence ◽  
Susceptible Population ◽  
In The Wild

AbstractTo explore resistant mechanism of wild oat to fenoxaprop-p-ethyl, the susceptibility of Acetyl-CoA Carboxylase (ACCase) from 24 wild oat populations to fenoxaprop-p-ethyl, the level of gene expression, and mutation site of ACCase were conducted. In vitro ACCase activities were solated and measured by enzyme-linked immunosorbent assay kit (ELISA) assays, the results indicated that the IC50 value of the ACCase of the most unsusceptible to fenoxaprop-p-ethyl in the wild oat population from Yexian2017 (W24) was 7206.557-fold compared to that of the ACCase of most susceptible to fenoxaprop-p-ethyl in the wild oat population from Queshan (W11). The differential expression of genes in wild oat treated by the IC50 fenoxaprop-p-ethyl concentration (6.9 mg/L) for 24 hours using RNA-seq, digital gene expression (DGE) profling was conducted. We found that 8 unigenes annotated as ACCase genes, 0 up-regulaed expression and 3 down-regulated expression were observed. The down-regulaed expressed ACCase was selected for qPCR in the relative susceptible population were significantly more suppressed than the three relative resistant populations. The mutations point of ACCase, Ile-1781-Leu, Trp-1999-Cys, Trp-2027-Cys, Ile-2041-Asn, Asp-2078-Gly, Cys-2088-Arg published were not found in the populations tested by multiple sequence alignment with a model complete ACCase sequence of Alopecurus myosuroides. These findings suggest that ACCase plays a critical role in the development of wild oat resistance to fenoxaprop-p-ethyl.

Selection and evolution of acetyl-CoA carboxylase (ACC)-inhibitor resistance in wild oat (Avena fatua L.) in a long-term alternative cropping systems study

2014 ◽  
Vol 94 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Hugh J. Beckie ◽  
Eric N. Johnson ◽  
Julia Y. Leeson ◽  
Scott W. Shirriff ◽  
Arlen Kapiniak
Keyword(s):  
Cropping Systems ◽  
Cropping System ◽  
Avena Fatua ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Evolution Of Resistance ◽  
Inhibitor Resistance ◽  
Herbicide Use

Beckie, H. J., Johnson, E. N., Leeson, J. Y., Shirriff, S. W. and Kapiniak, A. 2014. Selection and evolution of acetyl-CoA carboxylase (ACC)-inhibitor resistance in wild oat (Avena fatua L.) in a long-term alternative cropping systems study. Can. J. Plant Sci. 94: 727–731. In 2012, 18 yr after experiment establishment, wild oat from the spring wheat phase of seven of nine alternative cropping systems (each of three input levels applied to three levels of cropping diversity) were sampled and screened for ACC-inhibitor resistance. The frequency or level of resistance in wild oat was greatest in the diversified annual grains systems (42–60% of individuals), and lowest in the diversified annual perennial systems (<3%). The results of this study demonstrate the importance of perennial crops in slowing the selection and evolution of resistance in this weed. Moreover, annual cropping system diversity by itself is not enough to slow the evolution of ACC-inhibitor resistance in wild oat; cropping diversity must be linked with herbicide mode-of-action diversity and herbicide-use reduction.

Cross Resistance of Acetyl-coa Carboxylase (ACCase) Inhibitor–Resistant Wild Oat (Avena fatua) Biotypes in the Pacific Northwest

2008 ◽  
Vol 22 (1) ◽  
pp. 142-145 ◽  
Author(s):  
Ahmet Uludag ◽  
Kee Woong Park ◽  
Joshua Cannon ◽  
Carol A. Mallory-Smith
Keyword(s):  
Pacific Northwest ◽  
Avena Fatua ◽  
Cross Resistance ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
The Pacific

An isoleucine to leucine mutation in acetyl-CoA carboxylase confers herbicide resistance in wild oat

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1049-1056 ◽  
Author(s):  
Michael J Christoffers ◽  
Michelle L Berg ◽  
Calvin G Messersmith
Keyword(s):  
Herbicide Resistance ◽  
Dna Sequences ◽  
Resistance Mutation ◽  
Triticum Aestivum L ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Herbicide Resistant ◽  
Binding Domains ◽  
Substitution Site

Wild oat (Avena fatua L.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) represent an increasingly important weed control problem. The objective of this study was to determine the ACCase mutation responsible for herbicide resistance in a well-studied wild oat biotype (UM1). A 2039-bp region encompassing the carboxybiotin and acetyl-CoA binding domains of multifunctional plastidic ACCase was analyzed. DNA sequences representing three plastidic ACCase gene loci were isolated from both the resistant UM1 and a herbicide-susceptible biotype, consistent with the hexaploid nature of wild oat. Only one nonsynonymous point mutation was found among the resistant wild oat sequences, inferring an isoleucine to leucine substitution. The position of this substitution corresponds to residue 1769 of wheat (Triticum aestivum L.) plastidic ACCase (GenBank accession No. AF029895). Analysis of an F2 population derived from a cross between a herbicide-resistant and a susceptible biotype confirmed co-segregation of herbicide resistance with the mutated ACCase. We conclude that the isoleucine to leucine mutation is responsible for herbicide resistance in UM1 wild oat based on a comparison of the substitution site across species and ACCase types. While isoleucine is conserved among plastidic ACCases of herbicide-susceptible grasses, leucine is found in plastidic and cytosolic forms of multifunctional herbicide-resistant ACCase.Key words: acetyl-CoA carboxylase, herbicide resistance, mutation, wild oat.

A Seed Bioassay to Identify Acetyl-CoA Carboxylase Inhibitor Resistant Wild Oat (Avena fatua) Populations

1996 ◽  
Vol 10 (1) ◽  
pp. 85-89 ◽  
Author(s):  
Bruce G. Murray ◽  
Lyle F. Friesen ◽  
Kelly J. Beaulieu ◽  
Ian N. Morrison
Keyword(s):  
Root Development ◽  
Filter Paper ◽  
Agar Medium ◽  
Rapid Identification ◽  
Avena Fatua ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Quantitative Measurements ◽  
Low Levels

A seed bioassay was developed and tested for the rapid identification of aryloxyphenoxypropionate (APP) and cyclohexanedione (CHD) resistance in wild oat. Two susceptible (S) genotypes, UM5 and Dumont, were treated with fenoxaprop-P and sethoxydim over a range of dosages on filter paper and agar. The former is a wild oat line and the latter a tame oat cultivar. Within 5 d, shoot and root development of both genotypes were completely inhibited by 10 μM fenoxaprop-P and 5 μM sethoxydim. These dosages were then tested to determine if they were suitable for distinguishing between resistant (R) and susceptible (S) plants. Agar medium was preferred over filter paper because of the ease of preparation and maintenance. Four known R wild oat populations were included in the tests. Those with high levels of resistance produced significantly longer coleoptiles and roots than S genotypes, but those with moderate or low levels of resistance could not be separated statistically from S biotypes based on quantitative measurements. However, after exposing the germinating, treated seeds to light for 24 to 48 h, all the R populations produced green coleoptiles and initiated a first leaf, unlike the S genotypes which did not turn green or produce any new growth. This procedure proved useful in discriminating between R and S genotypes and in ranking populations in terms of relative levels of resistance.

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