Dynamin conditional knockout fibroblasts: Tamoxifen inducible Knockout method v1

2021 ◽  
Author(s):  
Daehun Park ◽  
Pietro De Camilli
Keyword(s):  
Cell Line ◽  
Conditional Knockout ◽  
Coated Pits ◽  
Line P

This cell line was described and characterized in the following paper: Ferguson, S.M., Raimondi, A., Paradise, S., Shen, H., Mesaki, K., Ferguson, A., Destaing, O., Ko, G., Takasaki, J., Cremona, O., O’Toole, E., De Camilli P. Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits. Developmental Cell 17, 811-822, 2009. PMID: 20059951]. This procedure describes tamoxifeninducible KO method using this cell line.

HTS-FTIR spectroscopy allows the classification of polyphenols according to their differential effects on the MDA-MB-231 breast cancer cell line

The Analyst ◽  
2017 ◽  
Vol 142 (8) ◽  
pp. 1244-1257 ◽  
Author(s):  
A. Mignolet ◽  
V. Mathieu ◽  
E. Goormaghtigh
Keyword(s):  
Breast Cancer ◽  
Ftir Spectroscopy ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Differential Effects ◽  
Line P

FTIR-based classification of the effect of polyphenols on a breast cancer cell line.

scholarly journals Synthesis, structural analysis, solution equilibria and biological activity of rhodium(iii) complexes with a quinquedentate polyaminopolycarboxylate

RSC Advances ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. 5282-5296 ◽  
Author(s):  
Marija S. Jeremić ◽  
Hubert Wadepohl ◽  
Vesna V. Kojić ◽  
Dimitar S. Jakimov ◽  
Ratomir Jelić ◽  
...  
Keyword(s):  
Biological Activity ◽  
Structural Analysis ◽  
Antitumor Activity ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cell Line ◽  
Solution Equilibria ◽  
Line P

Two new Rh(iii)–ed3a complexes [Rh(ed3a)(OH2)]·H2O and Na[Rh(ed3a)Cl]·H2O have shown good antitumor activity, especially against HeLa cell line.

scholarly journals Xanthones and anthraquinones from the soil fungus Penicillium sp. DWS10-P-6

RSC Advances ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 3162-3167
Author(s):  
Ya-jing Wang ◽  
Nan Ma ◽  
Chun-yue Liu ◽  
Yi-xuan Feng ◽  
Feng-xiang Zhang ◽  
...  
Keyword(s):  
Cell Line ◽  
Soil Fungus ◽  
Cytotoxic Activities ◽  
Penicillium Sp ◽  
Line P

Two new xanthones, oxisterigmatocystins J and K, and two new anthraquinones, versicolorins D and E, were characterized from the fungus Penicillium sp. Compounds 4 and 5 showed significant cytotoxic activities against HL-60 cell line.

scholarly journals Murine FSH Production Depends on the Activin Type II Receptors ACVR2A and ACVR2B

Endocrinology ◽  
2020 ◽  
Vol 161 (7) ◽  
Author(s):  
Gauthier Schang ◽  
Luisina Ongaro ◽  
Hailey Schultz ◽  
Ying Wang ◽  
Xiang Zhou ◽  
...  
Keyword(s):  
Cell Line ◽  
Bone Morphogenetic Protein ◽  
Litter Size ◽  
Knockout Mice ◽  
Conditional Knockout ◽  
Type Ii ◽  
Morphogenetic Protein ◽  
Testicular Weight ◽  
Critical Type

Abstract Activins are selective regulators of FSH production by pituitary gonadotrope cells. In a gonadotrope-like cell line, LβT2, activins stimulate FSH via the activin type IIA receptor (ACVR2A) and/or bone morphogenetic protein type II receptor (BMPR2). Consistent with these observations, FSH is greatly reduced, though still present, in global Acvr2a knockout mice. In contrast, FSH production is unaltered in gonadotrope-specific Bmpr2 knockout mice. In light of these results, we questioned whether an additional type II receptor might mediate the actions of activins or related TGF-β ligands in gonadotropes. We focused on the activin type IIB receptor (ACVR2B), even though it does not mediate activin actions in LβT2 cells. Using a Cre-lox strategy, we ablated Acvr2a and/or Acvr2b in murine gonadotropes. The resulting conditional knockout (cKO) animals were compared with littermate controls. Acvr2a cKO (cKO-A) females were subfertile (~70% reduced litter size), cKO-A males were hypogonadal, and both sexes showed marked decreases in serum FSH levels compared with controls. Acvr2b cKO (cKO-B) females were subfertile (~20% reduced litter size), cKO-B males had a moderate decrease in testicular weight, but only males showed a significant decrease in serum FSH levels relative to controls. Simultaneous deletion of both Acvr2a and Acvr2b in gonadotropes led to profound hypogonadism and FSH deficiency in both sexes; females were acyclic and sterile. Collectively, these data demonstrate that ACVR2A and ACVR2B are the critical type II receptors through which activins or related TGF-β ligands induce FSH production in mice in vivo.

scholarly journals Development of CeO2 nanodot encrusted TiO2 nanoparticles with reduced photocatalytic activity and increased biocompatibility towards a human keratinocyte cell line

2020 ◽  
Vol 8 (18) ◽  
pp. 4016-4028 ◽  
Author(s):  
Alexander Morlando ◽  
Marcela Chaki Borrás ◽  
Yaser Rehman ◽  
Shahnaz Bakand ◽  
Philip Barker ◽  
...  
Keyword(s):  
Photocatalytic Activity ◽  
Cell Line ◽  
Tio2 Nanoparticles ◽  
Ros Generation ◽  
Nanocomposite Particles ◽  
Human Keratinocyte ◽  
Human Keratinocyte Cell Line ◽  
Keratinocyte Cell ◽  
Line P ◽  
Sunscreen Products

Low photocatalytic CeO2/TiO2 nanocomposite particles with high UV attenuation and reduced ROS generation for application in sunscreen products.

scholarly journals Design, synthesis and biological evaluation of 1,2,3-triazole based 2-aminobenzimidazoles as novel inhibitors of LasR dependent quorum sensing in Pseudomonas aeruginosa

RSC Advances ◽  
2019 ◽  
Vol 9 (50) ◽  
pp. 29273-29292 ◽  
Author(s):  
Singireddi Srinivasarao ◽  
Adinarayana Nandikolla ◽  
Shashidhar Nizalapur ◽  
Tsz Tin Yu ◽  
Sravani Pulya ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Cell Line ◽  
Biological Evaluation ◽  
Design Synthesis ◽  
Hek 293 ◽  
Line P ◽  
Synthesis And Biological Evaluation ◽  
Hek 293 Cell Line

Out of 40 benzimdazoles, 12 exhibited potent QSI activity against P. aeruginosa6p, most active QSI is docked to LasR and is less toxic against HEK 293 cell line.

The biosynthesis of a graphene oxide-based zinc oxide nanocomposite using Dalbergia latifolia leaf extract and its biological applications

2020 ◽  
Vol 44 (5) ◽  
pp. 2166-2179 ◽  
Author(s):  
Elavarasan Nagaraj ◽  
Prakash Shanmugam ◽  
Kokila Karuppannan ◽  
Thirunavukkarasu Chinnasamy ◽  
Sujatha Venugopal
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Antibacterial Activities ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Cell ◽  
A549 Lung Cancer Cell ◽  
Line P ◽  
A549 Lung ◽  
Mcf 7

ZnO/GO nanocomposites were synthesized and their antibacterial activities assessed against various pathogens. They are potential antibacterial materials and significant anticancer activity against the MCF-7 breast cancer cell line compared to the A549 lung cancer cell line.

scholarly journals Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor μ2 kinase

2003 ◽  
Vol 163 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Antony P. Jackson ◽  
Alexander Flett ◽  
Carl Smythe ◽  
Lindsay Hufton ◽  
Frank R. Wettey ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Heavy Chain ◽  
Transferrin Receptor ◽  
Coated Pits ◽  
Permeabilized Cells ◽  
Clathrin Heavy Chain

Endocytic cargo such as the transferrin receptor is incorporated into clathrin-coated pits by associating, via tyrosine-based motifs, with the AP2 complex. Cargo–AP2 interactions occur via the μ2 subunit of AP2, which needs to be phosphorylated for endocytosis to occur. The most likely role for μ2 phosphorylation is in cargo recruitment because μ2 phosphorylation enhances its binding to internalization motifs. Here, we investigate the control of μ2 phosphorylation. We identify clathrin as a specific activator of the μ2 kinase and, in permeabilized cells, we show that ligand sequestration, driven by exogenous clathrin, results in elevated levels of μ2 phosphorylation. Furthermore, we show that AP2 containing phospho-μ2 is mainly associated with assembled clathrin in vivo, and that the level of phospho-μ2 is strongly reduced in a chicken B cell line depleted of clathrin heavy chain. Our results imply a central role for clathrin in the regulation of cargo selection via the modulation of phospho-μ2 levels.

A far-red to NIR emitting ultra-sensitive probe for the detection of endogenous HOCl in zebrafish and the RAW 264.7 cell line

2019 ◽  
Vol 17 (14) ◽  
pp. 3538-3544 ◽  
Author(s):  
Vijay Natarajan ◽  
Natesan Thirumalaivasan ◽  
Shu-Pao Wu ◽  
Velmathi Sivan
Keyword(s):  
Cell Line ◽  
Fluorescence Imaging ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Endogenous Fluorescence ◽  
Line P ◽  
Raw 264.7 Cell Line

We report the synthesis of a far-red to NIR emitting probe for its application to the endogenous fluorescence imaging of HOCl in zebrafish.

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