Extraction of Non-Structural Carbohydrates (Total Soluble Sugars + Starch) in Plant Tissues v1

2021 ◽  
Author(s):  
Lynn Doran ◽  
Amanda P. De Souza
Keyword(s):  
Liquid Fraction ◽  
Soluble Sugars ◽  
Leaf Tissue ◽  
Plant Tissues ◽  
Total Soluble Sugars ◽  
Ethanolic Extraction

Ethanolic extraction of total soluble sugars (liquid fraction) and starch (precipitated fraction) from leaf tissue.

scholarly journals Changes in Organic and Inorganic Osmolytes of Maize (Zea mays L.) by Sulfur Application Under Salt Stress Conditions

2018 ◽  
Vol 10 (12) ◽  
pp. 543 ◽  
Author(s):  
Alia Riffat ◽  
Muhammad Sajid Aqeel Ahmad
Keyword(s):  
Tissue Concentration ◽  
Soluble Sugars ◽  
Leaf Tissue ◽  
Enzymatic Reactions ◽  
Total Soluble Sugars ◽  
Organic Osmolyte ◽  
Maximum Improvement ◽  
Total Free Amino Acids ◽  
Salt Toxicity

Mineral nutrients have favourable potential in alleviation of salinity problem in plants. Sulfur has specific functions in regulating plant growth, metabolism, enzymatic reactions and osmolyte homeostasis in plants. Hence, an experiment was carried out to explore the role of sulfur in ameliorating salt toxicity in maize by changes in organic and inorganic osmolyte contents. A range of sulfur levels (40, 80 mM) were used to induce salinity tolerance in maize. Various treatments of salinity (25, 75 mM) were applied by using sodium chloride. Results revealed that glycine betaine, proline, total soluble sugars, total soluble proteins and total free amino acids contents were increased by applying salinity while the application of sulfur lowered the proline and increased other studied organic osmolyte contents in all studied maize organs (leaf, shoot, root). The maximum improvement in organic osmolyte contents were found at 40 mM sulfur, however, at 80 mM sulfur proline contents were reduced. Applied salinity increased leaf tissue concentration of Na+ and decreased that of K+, Ca2+, NO3-, PO43-, SO42- leading to a severely declined in K+/Na and Ca2+/Na+ ratio. However, application of sulfur reduced the Na+ contents and improved K+, Ca2+, NO3-, PO43-, SO42-, K+/Na+ and Ca2+/Na+ ratio in the salinity grown plants. Moreover, 40 mM level of sulfur was greatly effective in osmolyte homeostasis at all levels of salinity. This indicated that use of sulfur (40 mM) ameliorated the effect of salinity by changing organic and inorganic osmolyte contents in maize plants.

scholarly journals Seasonal Patterns of Carbohydrates and Proline in Jojoba Clones that Differ in Frost Susceptibility

HortScience ◽  
1990 ◽  
Vol 25 (1) ◽  
pp. 103-105 ◽  
Author(s):  
Mubarak S. Khalafalla ◽  
David A. Palzkill
Keyword(s):  
Seasonal Changes ◽  
Soluble Sugars ◽  
Leaf Tissue ◽  
Early Summer ◽  
Seasonal Patterns ◽  
Nonstructural Carbohydrates ◽  
Resistant Clone ◽  
Total Soluble Sugars ◽  
Total Nonstructural Carbohydrates ◽  
Sugar Levels

Total nonstructural carbohydrates (TNC), starch, total soluble sugars, sucrose, and proline concentrations were monitored for 18 months in leaf tissue of two jojoba [Simmondsia chinensis (Link) Schneider] clones that differ in frost susceptibility. Seasonal changes in TNC and starch concentrations, with maxima in the winter and minima in summer, were significant. Sugar levels decreased from fall to spring and increased during early summer. The more frost-resistant clone (C-1) had significantly higher sugar concentrations during most of the study than the less frost-resistant clone (C-2). Proline concentrations largely followed the trends found for TNC. The C-1 clone had the higher levels of proline, except when C-2 was frost-injured. Growth trends were similiar between C-1 and C-2, with a major growth flush from March to May. Relatively high levels of starch preceded growth flushes.

scholarly journals Activated Yeast Extract Enhances Growth, Anatomical Structure, and Productivity of Lupinus termis L. Plants under Actual Salinity Conditions

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 74
Author(s):  
Ragab S. Taha ◽  
Mahmoud F. Seleiman ◽  
Bushra Ahmed Alhammad ◽  
Jawaher Alkahtani ◽  
Mona S. Alwahibi ◽  
...  
Keyword(s):  
Salinity Stress ◽  
Yeast Extract ◽  
Field Experiments ◽  
Soluble Sugars ◽  
Substantial Improvement ◽  
Vital Role ◽  
Anatomical Structure ◽  
Saline Stress ◽  
Total Soluble Sugars ◽  
Seed Yields

Salinity is one of the most severe environmental stresses that negatively limits anatomical structure, growth and the physiological and productivity traits of field crops. The productivity of lupine plants is severely restricted by abiotic stress, particularly, salinity in arid and semiarid regions. Activated yeast extract (AYE) can perform a vital role in the tolerance of environmental stress, as it contains phytohormones and amino acids. Thus, field experiments were conducted to explore the potential function of active yeast extract (0, 50, 75, and 100 mL AYE L−1) in mitigating the harmful impacts of salinity stress (EC = 7.65 dS m−1) on anatomical structure, growth, and the physiological and productivity traits of two lupine cultivars: Giza 1 and Giza 2. The different AYE treatments resulted in a substantial improvement in studied attributes, for example the growth, anatomical, physiological characteristics, and seed yields of treated lupine cultivars compared with untreated plants. Among the AYE doses, 75 mL L−1 significantly improved plant growth, leaf photosynthetic pigments, total soluble sugars, total protein, and seed yields, and exposed the best anatomical attributes of the two lupine cultivars grown under saline stress. The exogenous application of 75 mL AYE L−1 was the most influential, and it surpassed the control results by 45.9% for 100-seed weight and 26.9% for seed yield per hectare. On the other hand, at a concentration of 75 mL L−1 AYE there was a decrease in the alkaloids and endogenous proline under the studied salinity stress conditions. Promoted salinity stress tolerance through sufficient AYE dose is a hopeful strategy to enhance the tolerance and improve productivity of lupine into salinity stress. Furthermore, the response of lupine to salinity stress appears to rely on AYE dose. The results proved that Giza 2 was more responsive to AYE than Giza 1, showing a better growth and higher yield, and reflecting further salinity tolerance than the Giza 1 cultivar.

Total Soluble Sugar Quantification from Ethanolic Plant Extracts v1

2021 ◽  
Author(s):  
Lynn Doran ◽  
Amanda P. De Souza
Keyword(s):  
Plant Extracts ◽  
Plant Tissue ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Total Soluble Sugars ◽  
Total Soluble Sugar ◽  
Tissue Extracts

Quantification of total soluble sugars (as glucose) in plant tissue extracts via the sulfuric phenol method adapted for 96 well plates.

scholarly journals PRODUÇÃOE CARACTERIZAÇÃO DOSFRUTOS DE ESPÉCIES DE PhysalisCULTIVADAS NO SEMIÁRIDO BAIANO

2018 ◽  
Vol 14 (3) ◽  
pp. 113-121
Author(s):  
Tamara Torres Tanan ◽  
Marilza Neves do Nascimento ◽  
Romeu da Silva Leite
Keyword(s):  
Protein Content ◽  
Reducing Sugars ◽  
Sugar Content ◽  
Soluble Sugars ◽  
Breeding Period ◽  
Total Soluble Sugars ◽  
Biochemical Characteristics ◽  
The Masses ◽  
Solanaceae Family

The Physalisbelongs to the Solanaceae family and has sweetish fruit that can be eaten fresh or processed. In Brazil only Physalis peruvianais commercialized, it is necessary research to encourage the cultivation of other promising speciesof the genus. The aim of this study was evaluate the productivity and the physical and biochemical characteristics of P. angulata, P.pubescensand P. ixocarpafruits, in different stages,in Feira de Santana, Bahia. Productivity and the number of fruits in the breeding period were evaluated.In the fruits, longitudinal (LD) and transverse (TD) diameters, the LD/TD ratio,the masses of fruit, the protein content,reducing sugars,and total soluble sugars were analyzed. The results showed differences between species, with higher number of fruits for P. angulata(140,5). P. ixocarpashowed higher productivity (9 t ha-1) and fruit with greater mass and diameter,however P. pubescensand P. angulatashowed round fruits and higher sugar content, desirable characteristics for fresh consumption and for industrialization.

scholarly journals Production of Pityrocarpa moniliformis (Benth.) Luckow & R.W. Jobson (Fabaceae) seedlings irrigated with saline water

2021 ◽  
Vol 25 (3) ◽  
pp. 182-188
Author(s):  
Adriana dos S. Ferreira ◽  
Caio C. P. Leal ◽  
Bruno da S. Guirra ◽  
Salvador B. Torres ◽  
Marco Porceddu ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Irrigation Water ◽  
Saline Water ◽  
Block Design ◽  
Soluble Sugars ◽  
Dry Mass ◽  
Initial Growth ◽  
Saline Irrigation ◽  
Total Soluble Sugars ◽  
Chlorophylls A And B

ABSTRACT Saline irrigation water at high levels causes disturbance in the growth of more sensitive plants. The objective of this research was to evaluate the initial growth of Pityrocarpa moniliformis seedlings under different electrical conductivity in irrigation water through physiological and biochemical analyses. The experiment was conducted in a greenhouse with five water electrical conductivities (0.5, 2.0, 4.0, 6.0 and 8.0 dS m-1) and four repetitions of 20 plants each, arranged in a randomized block design. Application of the treatments with irrigation water containing NaCl began at 30 days after sowing. To determine the behaviour of the species, the following variables were analysed: stem diameter, plant height, number of leaves, leaf area, Dickson quality index, shoot dry mass, root dry mass and total dry mass, as well as the biochemical variables, such as the concentrations of total soluble sugars, free proline and chlorophylls a and b. The increase in irrigation water salinity hampered the growth of P. moniliformis seedlings, with electrical conductivity of 0.5 dS m-1 being the limit for maximum production. The results also indicated that the deleterious effects of salt stress on P. moniliformis seedlings variables increase in concentrations of proline, total soluble sugars and betaine glycine.

scholarly journals Age-Related Changes of Some Chemical Components in the Leaves of Oriental Beech (Fagus orientalis Lipsky.)

2019 ◽  
Vol 10 (2) ◽  
pp. 117-124
Author(s):  
Nezahat Turfan ◽  
Sezgin Ayan ◽  
Esra Nurten Yer ◽  
Halil Barış Özel
Keyword(s):  
Phenolic Compounds ◽  
Soluble Protein ◽  
Soluble Sugars ◽  
Total Soluble Protein ◽  
Total Phenolic ◽  
Total Chlorophyll ◽  
H2o2 Concentration ◽  
Total Soluble Sugars ◽  
Oriental Beech ◽  
Different Ages

Background and Purpose: This study presents the analysis of photosynthetic pigments, proline, total soluble protein, total amino acids, glucose, sucrose, total soluble sugars, total amount of phenolic compounds and flavonoids, malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentration in the leaf samples collected from oriental beech trees, which are naturally spread in Kastamonu Province, Turkey, with differing ages, enzyme activities of ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD). Material and Methods: The research was carried out on oriental beech trees (Fagus orientalis L.) of different ages located at 1300 m high elevation in Ahlat Village of Kastamonu Province, Turkey. Oriental beech trees of different ages (≥25, ≥50, ≥100, ≥200 and ≥600 years-old) constituted the material of this study. In leaf samples taken from trees of different ages, photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid), proline, total soluble protein, total amino acid, glucose, sucrose, total soluble sugars, the amount of total phenolic compounds and flavonoids, MDA, H2O2 concentration, enzyme activities of APX, CAT and SOD, as well as the relationship between the total content of C, N and H elements and the tree ages were studied. Results: As a result of the research conducted, significant differences were determined in terms of chlorophyll, total phenolic compound, flavonoid, glucose, amounts of sucrose, nitrogenous compounds, proline, total soluble protein, MDA, H 2O2 concentrations, and the activities of APX, CAT and SOD in the leaves of oriental beech trees with differing ages. The highest content of chlorophyll a was found to be in the youngest age group of ≥25 years. Total chlorophyll is low in young trees and high in middle-aged, old and very old trees. According to the results obtained, it was concluded that the MDA and H2O2 concentrations in the trees did not vary depending on the age of trees only, but also on the genotype, environmental conditions and metabolic activities. It was concluded that the fact that the total chlorophyll, phenolic compounds and sucrose content in oriental beech trees are high and that MDA content is low could have an influence on the long life of ≥600 years-old oriental beech trees. Conclusions: The activity of photosynthesis is related to leaf characteristics more than the age of trees.

scholarly journals Effects of static magnetic field on growth, some biochemical and antioxidant system in lemon balm (Melissa officinalis L.) seedlings

2021 ◽  
Vol 20 (1) ◽  
pp. 71-80
Author(s):  
Anousheh Zakeri ◽  
Ramazan-Ali Khavari-Nejad ◽  
Sara Saadatmand ◽  
Fatemeh Nouri Kootanaee ◽  
Rouzbeh Abbaszadeh
Keyword(s):  
Magnetic Field ◽  
Phenolic Compounds ◽  
Static Magnetic Field ◽  
Plant Biomass ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Antioxidant Defense System ◽  
Total Soluble Sugars ◽  
Lemon Balm ◽  
Total Soluble Sugar

The effects of magnetic waves as natural environmental factors on the Earths are not well known on plant growth and development. The present study was carried out to evaluate the effects of static magnetic field (SMF) treatment (4 and 6 mT for 30 and 120 min per day) for eight days on the biomass production, proline contents and total soluble sugar, phenolic compounds, accumulation of H2O2 and MDA along with activity of antioxidant enzymes in lemon balm seedlings. Our results showed that SMF treatments, especially 6 mT and 120 min duration, increased the plant biomass, proline contents, phenolic compounds, H2O2 and MDA accumulation, and reduced the contents of total soluble sugars. The SMF application also increased the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzymes compared to untreated seedlings. Our results suggest that SMF treatments induces the antioxidant defense system in the lemon balm seedlings and, by changing the plant metabolism, improves the early vigor of seedlings.

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