scholarly journals Age-Related Changes of Some Chemical Components in the Leaves of Oriental Beech (Fagus orientalis Lipsky.)

2019 ◽  
Vol 10 (2) ◽  
pp. 117-124
Author(s):  
Nezahat Turfan ◽  
Sezgin Ayan ◽  
Esra Nurten Yer ◽  
Halil Barış Özel
Keyword(s):  
Phenolic Compounds ◽  
Soluble Protein ◽  
Soluble Sugars ◽  
Total Soluble Protein ◽  
Total Phenolic ◽  
Total Chlorophyll ◽  
H2o2 Concentration ◽  
Total Soluble Sugars ◽  
Oriental Beech ◽  
Different Ages

Background and Purpose: This study presents the analysis of photosynthetic pigments, proline, total soluble protein, total amino acids, glucose, sucrose, total soluble sugars, total amount of phenolic compounds and flavonoids, malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentration in the leaf samples collected from oriental beech trees, which are naturally spread in Kastamonu Province, Turkey, with differing ages, enzyme activities of ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD). Material and Methods: The research was carried out on oriental beech trees (Fagus orientalis L.) of different ages located at 1300 m high elevation in Ahlat Village of Kastamonu Province, Turkey. Oriental beech trees of different ages (≥25, ≥50, ≥100, ≥200 and ≥600 years-old) constituted the material of this study. In leaf samples taken from trees of different ages, photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid), proline, total soluble protein, total amino acid, glucose, sucrose, total soluble sugars, the amount of total phenolic compounds and flavonoids, MDA, H2O2 concentration, enzyme activities of APX, CAT and SOD, as well as the relationship between the total content of C, N and H elements and the tree ages were studied. Results: As a result of the research conducted, significant differences were determined in terms of chlorophyll, total phenolic compound, flavonoid, glucose, amounts of sucrose, nitrogenous compounds, proline, total soluble protein, MDA, H 2O2 concentrations, and the activities of APX, CAT and SOD in the leaves of oriental beech trees with differing ages. The highest content of chlorophyll a was found to be in the youngest age group of ≥25 years. Total chlorophyll is low in young trees and high in middle-aged, old and very old trees. According to the results obtained, it was concluded that the MDA and H2O2 concentrations in the trees did not vary depending on the age of trees only, but also on the genotype, environmental conditions and metabolic activities. It was concluded that the fact that the total chlorophyll, phenolic compounds and sucrose content in oriental beech trees are high and that MDA content is low could have an influence on the long life of ≥600 years-old oriental beech trees. Conclusions: The activity of photosynthesis is related to leaf characteristics more than the age of trees.

scholarly journals Effect of UV-B Radiation on Germination of Maize (Zea mays L.)

2017 ◽  
Vol 14 (3) ◽  
pp. 887-891
Author(s):  
Nagendram Erram ◽  
Anil Gaddameedi ◽  
Swapna Siddamalla ◽  
Tumu Venkat Reddy ◽  
Manjula Bhanoori
Keyword(s):  
Amino Acids ◽  
Soluble Protein ◽  
Free Amino Acids ◽  
Free Amino ◽  
Soluble Sugars ◽  
Germination Percentage ◽  
Total Soluble Protein ◽  
Total Soluble Sugars ◽  
Maize Seeds ◽  
Proline Concentration

ABSTRACT: In the present investigation, Maize seeds of hybrid NK 6240 and 900M Gold were exposed to UV-B (280-320 nm) for periods of 40 and 60 minutes and compared with the control without exposer to UV-B. The biochemical changes associated with UV-B induced resistance were investigated by determination of proline concentration, total soluble sugars, total soluble protein, malondialdehyde content and free amino acids from leaves and roots. Also comparison for germination percentage between control and treated seeds was carried along with biochemical traits. Analysis of variance explains both the hybrids were significantly different in germination percentage, total soluble sugars, proline and free amino acids. Whereas both treatments showed high significant variation for all the studied traits, but genotype × treatment interaction was non-significant for all the traits. 40 minutes UV-B treated seeds showed reduced total soluble sugars and increased malondialdehyde, proline and total soluble protein content. In 60 minutes UV-B treatment, decrease in free amino acids, proline, percentage of germination, and total soluble protein and increase in total soluble sugars was observed.

scholarly journals Responses to Drought in Seedlings of European Larch (Larix decidua Mill.) from Several Carpathian Provenances

Forests ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 511
Author(s):  
Ioana M. Plesa ◽  
Mohamad Al Hassan ◽  
Sara González-Orenga ◽  
Adriana F. Sestras ◽  
Oscar Vicente ◽  
...  
Keyword(s):  
Drought Stress ◽  
Phenolic Compounds ◽  
Early Detection ◽  
Photosynthetic Pigments ◽  
Soluble Sugars ◽  
Larix Decidua ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
European Larch ◽  
Total Soluble Sugars

European larch (Larix decidua Mill.) has been reported either as more tolerant or as more sensitive to drought than conifers with perennial leaves. Previous studies have revealed that Carpathian populations of European larch display a high genetic variability. A comparative study of the responses of these populations to drought stress at the seedling stage might allow the identification of drought tolerant genotypes and reliable drought stress biomarkers, which could be eventually used for the early detection of drought effects in larch, not only under control greenhouse conditions, but also in their natural stands. Growth responses were analyzed in larch seedlings from six Romanian Carpathian populations, submitted to one month of mild drought stress under controlled conditions. Levels of photosynthetic pigments (chlorophylls a and b, and carotenoids), osmolytes (proline and total soluble sugars), monovalent cations (Na+ and K+), and malondialdehyde (MDA) and non-enzymatic antioxidants (total phenolics and flavonoids) were compared with control treatments and between populations. Growth and the pattern of the biochemical responses were very similar in the six populations. Drought stress lead to stem length decrease in all population, whereas reduction of fresh weight of needles was significant only in one population (BVVC), and reduction of water content of needles in two populations (BVVC and GuHo). The optimal biochemical traits for an early detection of drought symptoms in this species is the increase—in most populations—of total soluble sugars, MDA, and total phenolic compounds, whereas K+ reduction was significant in all populations. Photosynthetic pigments remained unchanged, except for the Anin population where they were reduced under stress. Multivariate principal component and hierarchical clustering analyses confirmed the impact of drought in the growth and physiology of European larch, and revealed that the humidity of the substrate was positively correlated with the growth parameters and the levels of K+ in needles, and negatively correlated with the levels of MDA, total soluble sugars, total phenolic compounds, and flavonoids in needles.

scholarly journals Osmolyte accumulation and antioxidant metabolism during germination of vigorous maize seeds subjected to water deficit

2020 ◽  
Vol 42 ◽  
pp. e42476
Author(s):  
Camila Segalla Prazeres ◽  
Cileide Maria Medeiros Coelho
Keyword(s):  
Antioxidant Enzymes ◽  
Water Deficit ◽  
Soluble Protein ◽  
Soluble Sugars ◽  
Principal Component ◽  
Seed Vigor ◽  
Total Soluble Protein ◽  
Total Soluble Sugars ◽  
Maize Hybrids ◽  
Osmotic Potentials

The objective of this work was to evaluate the alterations of antioxidant enzyme reserves and antioxidant enzymes during germination under water deficit in maize hybrids and to associate with seed vigor, determining the mechanisms related to tolerance for this stress. Two three-way maize hybrids were characterized by their vigor at different levels of water deficit induced by polyethylene glycol 6000. Next, the seeds were hydrated at different osmotic potentials (0.0, -0.3, and -0.9 MPa) and removed at different times to assess the levels of the total soluble protein, total soluble sugars, proline, starch, and antioxidant enzymes, such as superoxide dismutase, catalase and ascorbate peroxidase. The analysis of variance, Tukey test at 5% and principal component analysis (PCA) were used. The vigorous hybrid (HT1) was more efficient than the low vigor hybrid seeds (HT2) in mobilizing the total soluble protein during the initial stages of germination and the total soluble sugars before and after root protrusion under water deficit in addition to increasing the catalase activity at the different osmotic potentials that were assessed.

scholarly journals Impact of Cold Storage on Bioactive Compounds and Their Stability of 36 Organically Grown Beetroot Genotypes

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1281
Author(s):  
Khadijeh Yasaminshirazi ◽  
Jens Hartung ◽  
Michael Fleck ◽  
Simone Graeff-Hönninger
Keyword(s):  
Phenolic Compounds ◽  
Cold Storage ◽  
Significant Influence ◽  
Dry Matter ◽  
Soluble Sugars ◽  
Storage Period ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Total Soluble Sugars

In order to exploit the functional properties of fresh beetroot all year round, maintaining the health-benefiting compounds is the key factor. Thirty-six beetroot genotypes were evaluated regarding their content of total dry matter, total phenolic compounds, betalain, nitrate, and total soluble sugars directly after harvest and after cold storage periods of one and four months. Samples were collected from two field experiments, which were conducted under organic conditions in Southwestern Germany in 2017 and 2018. The outcome of this study revealed a significant influence of genotype (p < 0.05) on all measured compounds. Furthermore, significant impacts were shown for storage period on total dry matter content, nitrate, and total phenolic compounds. The medians of nitrate content based on the genotypes studied within the experiment ranged between 4179 ± 1267–20,489 ± 2988 mg kg−1 DW (dry weight), and that for the total phenolic compounds varied between 201.45 ± 13.13 mg GAE 100 g−1 DW and 612.39 ± 40.58 mg GAE 100 g−1 DW (milligrams of gallic acid equivalents per 100 g of dry weight). According to the significant influence of the interactions of storage period and genotype on total soluble sugars and betalain, the decrease or increase in the content of the assessed compounds during the cold storage noted to be genotype-specific. Therefore, to benefit beetroots with retained quality for an extended time after harvest, selection of the suitable genotype based on the intended final use is recommended.

scholarly journals Variation in Chemicals and Growth Parameters of Taşköprü Garlic

2020 ◽  
Vol 8 (4) ◽  
pp. 847-854
Author(s):  
Nezahat Turfan
Keyword(s):  
Amino Acid ◽  
Bioactive Compounds ◽  
Soluble Protein ◽  
Amylase Activity ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Total Phenolic ◽  
Glucose Content ◽  
Proline Amino Acid ◽  
Β Carotene

Main goals of the present study were (1) to initially investigate the nutrient contents and bioactive compounds in the bulb and cloves of garlic, and (2) to study the growth parameter after planting. Garlic bulbs were firstly separated into three categories as pickled, big and small, while the big garlic cloves were also classified into three categories as big, small and central. Secondly, the garlic samples were analyzed before planting for their element profile, proline, soluble protein, free amino acid, β-carotene, lycopene, total phenolic, soluble sugars, SOD and α-amylase activities. Finally, the growth parameters were measured using the cultivated cloves and the pickled bulbs. According to the result, the highest soluble protein, N, phenolic, lycopene and α amylase activity (97.06 mg, 2.58%, 971 mg, 0.368 mg and 38.13 EU, respectively) were recorded in the biggest cloves. The highest proline, amino acid, glucose content (93.84 µmol, 23.54 mg, 230.89 mg, respectively) and K, P, S, Mg, Mn, Fe and Zn (21940 ppm, 7577 ppm, 12200 ppm, 504 ppm, 38.1ppm, 377,7ppm and 44.5 ppm, respectively) were found in the pickled bulb. The maximum level of β-carotene (0.282 mg), Ca, Cl and Sr (11260ppm, 818.7ppm and 47.9 ppm) were determined in the small bulbs. Based on the growth parameters of seedlings, the highest value of shoot and root length (39.12 cm and 24.11 cm respectively), the fresh weight of shoot and root (5.29 g and 4.54 g respectively) and dry weight of shoot and root (1.70 g and 1.24 g respectively) were noted with the big cloves. The results of the current study have indicated that the pickled cloves have higher macro and micro nutrients, proline, amino acid and glucose, while the big cloves of garlic have higher proline, phenolic, N%, lycopene and amylase activity. It can be said that the big cloves showed good value for the five bioactive compounds, but the pickled exhibited good value for the macro and micro element and glucose.

scholarly journals Effects of static magnetic field on growth, some biochemical and antioxidant system in lemon balm (Melissa officinalis L.) seedlings

2021 ◽  
Vol 20 (1) ◽  
pp. 71-80
Author(s):  
Anousheh Zakeri ◽  
Ramazan-Ali Khavari-Nejad ◽  
Sara Saadatmand ◽  
Fatemeh Nouri Kootanaee ◽  
Rouzbeh Abbaszadeh
Keyword(s):  
Magnetic Field ◽  
Phenolic Compounds ◽  
Static Magnetic Field ◽  
Plant Biomass ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Antioxidant Defense System ◽  
Total Soluble Sugars ◽  
Lemon Balm ◽  
Total Soluble Sugar

The effects of magnetic waves as natural environmental factors on the Earths are not well known on plant growth and development. The present study was carried out to evaluate the effects of static magnetic field (SMF) treatment (4 and 6 mT for 30 and 120 min per day) for eight days on the biomass production, proline contents and total soluble sugar, phenolic compounds, accumulation of H2O2 and MDA along with activity of antioxidant enzymes in lemon balm seedlings. Our results showed that SMF treatments, especially 6 mT and 120 min duration, increased the plant biomass, proline contents, phenolic compounds, H2O2 and MDA accumulation, and reduced the contents of total soluble sugars. The SMF application also increased the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzymes compared to untreated seedlings. Our results suggest that SMF treatments induces the antioxidant defense system in the lemon balm seedlings and, by changing the plant metabolism, improves the early vigor of seedlings.

scholarly journals Biochemical and phytochemical evaluation of Stevia rebaudiana

2016 ◽  
Vol 2 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Md Moniruzzaman Sohag Howlader ◽  
Sheikh Rashel Ahmed ◽  
Khadizatul Kubra ◽  
Md Khairul Hassan Bhuiyan
Keyword(s):  
Phenolic Compounds ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Stevia Rebaudiana ◽  
Reducing Sugar ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Dry Extract ◽  
Total Soluble Sugar

The present study was aimed to evaluate biochemical and phytotochemical of dry leaves of Stevia rebaudiana. Biochemical analysis indicated that Stevia leaves are a good source of carbohydrate and reducing sugar. Different extraction methods were used to prepare four different dry extracts (Extract A, B, C, D). Total soluble sugars and reducing sugars were analyzed for these four dry extracts and commercial Stevia powder. The highest amount of total soluble sugar (477 mg sugar g-1 dry extract) was obtained from extract C and higher amount of reducing sugar (82 mg g-1 dry extract) was obtained from extract D among the extracts. But commercial Stevia powder showed higher total soluble sugar content (754 mg g-1 dry powder) and highest amount of reducing sugar (98 mg g-1 dry extract) than all the extracted dry samples. The extraction process of dry extract C was feasible for the extraction of total soluble sugar. For the phytochemical screening, crude extract was tested for the presence of different chemical groups and presence of alkaloids, phenolic compounds, steroids, tannins, flavonoids, cardiac glycosides and saponins that were identified. The highest amount of total phenolic compounds (92 mg) was recorded from methanolic extract of extraction B. The lowest amount of total phenolic compounds (36 mg) was recorded in ethanolic extract of extraction A. So, Methanol proved as best solvent to extract increased quantity of total phenolic compounds than other solvents.Asian J. Med. Biol. Res. March 2016, 2(1): 121-130

scholarly journals Hydropriming as inducer of salinity tolerance in sunflower seeds

2018 ◽  
Vol 22 (4) ◽  
pp. 255-260 ◽  
Author(s):  
Janete R. Matias ◽  
Salvador B. Torres ◽  
Caio C. P. Leal ◽  
Moadir de S. Leite ◽  
Sara M. C. Carvalho
Keyword(s):  
Salinity Tolerance ◽  
Dry Matter ◽  
Soluble Sugars ◽  
Total Chlorophyll ◽  
Sunflower Seeds ◽  
Soluble Salts ◽  
Fresh Matter ◽  
Total Soluble Sugars ◽  
Electrical Conductivities ◽  
Semi Arid

ABSTRACT Sunflower is a species with multiple potentialities and its yield can be affected by the presence of soluble salts in the soil, which is common in arid, semi-arid and irrigated regions. The objective of this study was to evaluate the effect of hydropriming as inducer of salinity tolerance in seeds and seedlings of sunflower, hybrid MG305CP. The germination tests were set with four replicates of 50 seeds per treatment, maintained in germinator at 30 ºC for ten days. To simulate salinity, sodium chloride diluted in distilled water was used at electrical conductivities of 0 (control), 4, 8, 12 and 16 dS m-1. On the tenth day, the number of normal seedlings was counted and the length and dry matter of shoots and roots were determined. Seedling fresh matter was used to determine the contents of total chlorophyll, total soluble sugars and proline. The experimental design was completely randomized, in a 2 x 5 factorial scheme (with and without hydropriming and five levels of salinity). Hydroprimed sunflower seeds showed greater vigor expression, with higher number of normal seedlings and faster germination.

scholarly journals The response of cucumber plants (Cucumis sativus L.) to the application of PCB-contaminated sewage sludge and urban sediment

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6743 ◽  
Author(s):  
Anna Wyrwicka ◽  
Magdalena Urbaniak ◽  
Mirosław Przybylski
Keyword(s):  
Sewage Sludge ◽  
Chlorophyll Content ◽  
Cucumis Sativus ◽  
Chlorophyll A ◽  
Soluble Protein ◽  
Municipal Wastewater ◽  
Total Soluble Protein ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Antioxidative Response

Background The increasing production of sewage sludge (SS) engenders the problem of its responsible utilization and disposal. Likewise, urban sediments (SED) are deposited at the bottom of urban reservoirs and sedimentation ponds, and these require periodical dredging and utilization. However, while the SS and SED deposits often contain nutrients such as nitrogen and phosphorus; however, they also contain a variety of hazardous compounds including heavy metals, Persistent Organic Pollutants (POPs) and microbial pollutants. Fortunately, some species of Cucurbitaceae can accumulate high levels of POPs, such as polychlorinated dibenzo-p-dioxins (PCDD), polychlorinated dibenzofurans (PCDF) and polychlorinated biphenyls (PCB), in their tissues. Methods SS was collected from the Lodz Municipal Wastewater Treatment Plant and SED from the Sokołówka Sequential Biofiltration System. The SS and SED samples were added to soil in flower pots at three concentrations (1.8 g, 5.4 g and 10.8 g per flower pot), and one pot was left as an unamended control (C). Soil PCB concentrations were determined before cucumber planting, and after five weeks of growth. Also, total soluble protein, total chlorophyll content, chlorophyll a/b ratio and degree of lipid peroxidation (TBARS) were examined in the leaves of the cucumber plants (Cucumis sativus L.) cv. Cezar after five weeks. Antioxidative response was assessed by ascorbate peroxidase (APx) and catalase (CAT) assay. Results The initial PCB concentration in soil after application of SS or SED was dependent on the applied dose. After five weeks, PCB concentration fell significantly for all samples and confirmed that the dose of SS/SED had a strong effect. Soil remediation was found to be more effective after SS application. Total soluble protein content in the cucumber leaf tissues was dependent on both the type and the dose of the applied amendments, and increased with greater SS doses in the soil. The total chlorophyll content remained unchanged, and the chlorophyll a/b ratio was slightly elevated only after the application of the highest SS and SED dose. The use of SS and SED did not significantly affect TBARS content. APx activity fell after SS or SED application; however, CAT activity tended to increase, but only in the leaves of plants grown in SS-amended soil. Discussion The cultivation of cucumber plants reduces PCB concentration in soil amended with SS or SED; however, this effect is more evident in the case of SS. SS application also induced more intensive changes in the activity of enzymes engaged in antioxidative response and oxidative stress markers in plant tissues than SED. The levels of PCB in the SS may have triggered a more severe imbalance between pro- and antioxidative reactions in plants. Cucumber plants appear to be resistant to the presence of toxic substances in SS and SED, and the addition of SS and SED not only acts as a fertilizer, but also protects against accelerated aging.

scholarly journals Screening for drought tolerance in cultivars of the ornamental genusTagetes(Asteraceae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2133 ◽  
Author(s):  
Raluca Cicevan ◽  
Mohamad Al Hassan ◽  
Adriana F. Sestras ◽  
Jaime Prohens ◽  
Oscar Vicente ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drought Tolerance ◽  
Plant Growth ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Stem Length ◽  
Total Phenolic ◽  
Total Soluble Sugars ◽  
Drought Tolerant ◽  
Components Analysis

Drought tolerance was evaluated in twelve cultivars of three ornamentalTagetesspecies (T. patula,T. tenuifoliaandT. erecta). A stress treatment was performed by completely stopping watering of plants maintained in controlled greenhouse conditions. After three weeks, several plant growth parameters (stem length (SL), fresh weight (FW) and water content (WC)), photosynthetic pigments (chlorophylls and carotenoids (Car)), osmolytes (proline (Pro), glycine betaine (GB) and total soluble sugars (TSS)), an oxidative stress maker (malondialdehyde (MDA)) and antioxidants (total phenolic compounds (TPC) and total flavonoids (TF)) were measured. Considerable differences in the evaluated traits were found among the control and drought-stressed plants. Drought stress generally caused a marked reduction in plant growth and carotenoid pigments, and an increase in soluble solutes and oxidative stress. For most cultivars, proline levels in stressed plants increased between 30 and 70-fold compared to the corresponding controls. According to the different measured parameters, on averageT. erectaproved to be more tolerant to drought thanT. patulaandT. tenuifolia. However, a considerable variation in the tolerance to drought was found within each species. The traits with greater association to drought tolerance as well as the most tolerant cultivars could be clearly identified in a principal components analysis (PCA). Overall, our results indicate that drought tolerant cultivars ofTagetescan be identified at early stages using a combination of plant growth and biochemical markers.

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