In vitro antimicrobial activity of Caesalpinia coriaria (Jacq.) Willd extracts on Streptococcus pyogenes and Candida albicans

Background: “Dividivi” Caesalpinia coriaria (Jacq.) Willd fruits are traditionally used by the Wayuú community in La Guajira (Colombia) to treat oral and skin cavity diseases caused by bacteria and fungi. Streptococcus pyogenes is a gram-positive cocci of group A (beta-hemolytic) that is the cause of pharyngeal disease, scarlet fever, cellulitis, erysipelas, or toxic shock-like syndrome. Alternatively, Candida albicans is a yeast-like fungus that is a normal flora of the digestive tract, vagina, or skin folds; it has been known to be the root cause of opportunistic diseases such as diaper rash, oral and esophagus thrush, or vulvovaginitis. Objective: This study evaluated the antimicrobial activity of methanolic and ethanolic extracts of C. coriaria (Jacq.) Willd dry fruits on S. pyogenes ATCC 12384 and C. albicans ATTC 14053. Method: C. coriaria extracts were obtained from the Soxhlet method using two solvents (methanol and ethanol 98%) prepared from pulverized fruits. A phytochemical test and an antimicrobial activity assay were performed using the obtained extracts and tested using S. pyogenes ATCC 12384 and C. albicans ATTC 14053 strains. Results: A phytochemical profile was performed, examining the presence of bioactive metabolites (tannins, alkaloids, glycosides, saponins, and anthraquinones) from each extract. Antimicrobial susceptibility tests showed that the ethanolic extract inhibited S. pyogenes ATCC 12384, causing inhibition halos of 14.1 ± 0.1 mm and a Minimum Inhibitory Concentration (MIC) of 172 mg/ml, and C. albicans test shows inhibition halos of 16.1 ± 0.2 mm and MIC of 212 mg/ml. Additionally, the methanolic extract inhibited S. pyogenes with inhibition halos of 15.2 ± 0.2 mm and MIC of 152 mg/ml; no inhibitory effect was observed on C. albicans. Conclusion: This study revealed that C. coriaria has an antimicrobial effect on the tested species opening the field of its possible use as a therapeutic agent.

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Comparative studies of the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola on Klebsiella pneumonia and Candida albicans in vitro

Nigerian Journal of Technological Research ◽

10.4314/njtr.v16i1.7 ◽

2021 ◽

Vol 16 (1) ◽

pp. 52-58

Author(s):

Elizabeth Winful ◽

Olanikpekun Idowu ◽

Opeoluwa O. Fasanya ◽

Nkechi E. Egbe

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Ethanol Extract ◽

Antimicrobial Effect ◽

Klebsiella Pneumonia ◽

Disc Diffusion ◽

Diffusion Test ◽

Garcinia Kola ◽

Zones Of Inhibition

Antimicrobial resistance has become a global health problem. Although a wide range of chemotherapeutic antimicrobials are available for treatment of microbial related infections and diseases, development of resistance to these chemotherapeutic agents is rapidly on the increase. Extracts from some plants have shown some promise in antimicrobial activity. This has led to the screening of several medicinal plants for their potential antimicrobial activity. Therefore, this study was aimed at evaluating the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola against Klebsiella pneumonia and Candida albicans in vitro. The Garcinia kola seeds extracts were obtained using 70% ethanol and distilled water respectively. Phytochemical screening of Garcinia kola revealed the presence of various potent phytochemicals such as tannins, saponins, flavonoids, alkaloids and glycosides. Both extracts of the seeds were investigated for antimicrobial activity using disc diffusion and agar well diffusion sensitivity tests. The ethanol extract produced zones of inhibition of about 7.3 mm for Candida albicans only at a concentration of 800mg/ml for the disc diffusion test. For the agar well diffusion test, the aqueous extract produced zones of inhibition of about 9.5 mm, while the ethanol extract produced zone of inhibition of 19 mm against Candida albicans at a concentration of 800 mg/ml. However, at the same concentration, the ethanol extract produced zones of inhibition of about 8.5 mm against Klebsiella pneumoniae. The findings of this study revealed that Garcinia kola was not effective in treating Klebsiella pneumonia infections but has potential in treating Candida albicans infections. Keywords: Garcinia kola, Klebsiella pneumonia, Candida albicans

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Antifungal Activity of Beluntas “Indian Camphorweed” (Pluchea indica) Ethanol Extract on Candida albicans In Vitro Using Different Solvent Concentrations

European Journal of Dentistry ◽

10.1055/s-0041-1736591 ◽

2021 ◽

Author(s):

Wayan Larissa Demolsky ◽

Vinna Kurniawati Sugiaman ◽

Natallia Pranata

Keyword(s):

Candida Albicans ◽

Oral Candidiasis ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Pathogenic Fungi ◽

Inhibitory Effect ◽

Plate Count ◽

Minimum Fungicidal Concentration ◽

Significant Difference

Abstract Objective Oral candidiasis is an infection caused by pathogenic fungi Candida albicans, with a considerably high prevalence of 20 to 72%. Indian camphorweed (Pluchea indica) also known as “beluntas” as the local name has been known as a traditional medicine in Indonesia. The objective of this study is to research the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of beluntas ethanolic extract against the growth of C. albicans. Materials and Methods The MIC and MFC were measured by microdilution assay and total plate count respectively with a variation of solvents (DMSO 1%, 10%, and 4%) and beluntas extract with concentrations between 0.3125 and 200 mg/mL. Amphotericin and nystatin were used as a comparison. Statistical Analysis One-way analysis of variance and posthoc Tukey test were used to determine the significant difference between treatments. Results It was found that the MIC ranged from 50 to 200 mg/mL in the test with DMSO 10% solvent and MFC was found to be at a concentration of 200 mg/mL. However, there is a significant inhibitory effect and killing effect from DMSO 10% against C. albicans (p = 0.000). MIC was also found within concentrations of 100 mg/mL of beluntas extract in DMSO 4%. In this study, the DMSO 4% concentration neither showed significant inhibitory effects nor killing effects; therefore, the result was acceptable (p = 0.357). Conclusion Ethanol extract of beluntas (P. Indica) has the potential of being an antifungal agent with inhibitory activity in concentrations ≥100 mg/mL, which is similar to nystatin (p = 0.278). The MFC for the extract was above 100 mg/mL, which cannot be measured with this method as a higher concentration of DMSO is needed, which had a toxic effect on the tested fungi.

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ANTIMICROBIAL ACTIVITY OF 3- (1- (3- HYDROXYPHENYL) AMINO) ETHYLIDENE) CHROMAN-2,4-DIONE AND ITS CORRESPONDING PALLADIUM(II) COMPLEX

10.46793/iccbi21.387a ◽

2021 ◽

Author(s):

Edina Avdović ◽

◽

Žiko B. Milanović ◽

Milanka Radulović ◽

Dušan S. Dimić

Keyword(s):

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Antimicrobial Effect ◽

Coumarin Derivative ◽

The Other ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Microdilution Method ◽

Bacteria And Fungi

In this manuscript, the in vitro antimicrobial activity of the previously synthesized coumarin derivative 3- (1- (3-hydroxyphenyl) amino) ethylidene) chroman-2,4-dione (L) and its corresponding palladium (II) complex (C) were examined. Their antimicrobial activity was screened against four strains of bacteria Bacillus cereus (ATCC 11778) G+; Staphylococcus aureus (ATCC 13709) G+; Klebsiella pneumoniae (ATCC 27736) G-; and Escherichia coli (ATCC 2592) G-) and three strains of fungi (Aspergillus flavus (ATCC15517); Candida albicans (ATCC 10231); Fusarium oxysporum (ATCC 695) using disc diffusion and microdilution method. The obtained minimum inhibitory concentration (MIC) values by microdilution method for ligand and complex are similar for all tested bacteria and fungi, which means that both compounds have a similar antimicrobial effect. On the other hand, analysis of zone of inhibition (ZI) values for the tested compounds shows that the complex is generally somewhat more active than the ligand.

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Synthesis and in vitro antimicrobial activity of new 3-(2-morpholinoquinolin-3-yl) substituted acrylonitrile and propanenitrile derivatives

Chemical Papers ◽

10.2478/s11696-011-0048-8 ◽

2011 ◽

Vol 65 (5) ◽

Cited By ~ 4

Author(s):

Jigar Makawana ◽

Manish Patel ◽

Ranjan Patel

Keyword(s):

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Double Bond ◽

Fungal Pathogen ◽

Condensation Reaction ◽

Clostridium Tetani ◽

Gram Positive Bacteria ◽

Bacteria And Fungi

AbstractA series of new 3-(2-morpholinoquinolin-3-yl)acrylonitrile derivatives (IVa-IVf) has been synthesised by the base-catalysed condensation reaction of 2-morpholinoqionoline-3-carboxaldehydes (IIa-IIc) and 2-cyanomethylbenzimidazoles (IIIa-IIIb). Subsequent regiospecific reduction of the C—C double bond in acrylonitrile moiety afforded 3-(2-morpholinoquinolin-3-yl)propanenitrile derivatives (Va-Vf). All the compounds synthesised were subjected to in vitro antimicrobial screening against some representatives of bacteria and fungi. The majority of the compounds were found to be active against Gram-positive bacteria Bacillus subtilis and Clostridium tetani as well as against the fungal pathogen Candida albicans.

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Evaluation of antimicrobial activity of magnesium oxide nanocomposite film in combination with ε-poly-L-lysine against Foodborne pathogens in vacuum packaged beef

10.21203/rs.3.rs-294697/v1 ◽

2021 ◽

Author(s):

Masumeh Samadi ◽

Seyed Shahram Shekarforoush ◽

Hamid Reza Gheisari

Keyword(s):

Antimicrobial Activity ◽

Magnesium Oxide ◽

Culture Media ◽

Antimicrobial Effect ◽

Inhibitory Effect ◽

Melt Mixing ◽

E Coli ◽

Mgo Nanoparticle

Abstract Background: The aim of this study was to evaluate the antimicrobial activity of magnesium oxide nanocomposite (MgO NC) film based on Low-density polyethylene (LDPE) alone or in combination with three concentrations of ε-poly-L-lysine (500, 1000 and 2000 µg/ml) on Escherichia coli O157:H7 and Listeria monocytogenes in culture media and fresh beef. Methods: MgO NC film were prepared by melt mixing LDPE and MgO nanoparticle in the extruder. For in vitro antibacterial analysis, the MgO NC film alone or in combination with polylysine were evaluated in tryptic soy broth for 5 days at 37 °C. For in vivo analysis, beef samples were inoculated with the selected bacteria and packaged in MgO NC film under vacuum and stored at 4 °C and evaluated for up to 20 days. Results: Polylysine had an antibacterial effect against E. coli and L. monocytogenes in TSB. But MgO NC film had a bacteriostatic effect only against E. coli. MgO NC film inhibited the growth of E. coli on the surface of beef samples. Pollysine at concentrations of 500 µg/ml or more showed inhibitory activity against E. coli and L. monocytogenes in beef samples. No additional reduction was observed by combining the different concentrations of polylysine with MgO NC film.Conclusions: Polylysine at all concentrations had an inhibitory effect on E. coli and L. monocytogenes in the culture medium and beef. Although the migration of MgO nanoparticle from the film to beef was very low, but as it has little antimicrobial effect, it is not recommended as a suitable package for improving the safety of raw beef.

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Screening of Antimicrobial Activity of Siddha drug Kukkiladhi Choornam

Hygeia journal for drugs and medicines ◽

10.15254/h.j.d.med.10.2018.176 ◽

2018 ◽

Vol 10 (1) ◽

pp. 40-42

Author(s):

Marimuthu Revathi ◽

Murugesan Karthikeyan ◽

Ashok Gnanasekaran ◽

Ranjith MS

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Streptococcus Pyogenes ◽

Shigella Flexneri ◽

Salmonella Typhi ◽

Antibacterial And Antifungal Activity ◽

Proteus Vulgaris ◽

Solid Media ◽

Antibacterial And Antifungal

Plan: Kukkiladhi choornam is one of the Siddha drugs, which has been indicated for its anti-microbial properties. The aim of the present study was to validate the antibacterial and antifungal activity of Kukkiladhi choornam extract against various microorganisms. Preface: The present study examined the in-vitro screening of antimicrobial activity of Siddha drug Kukkiladhi choornam. Methodology: The extracts were tested for antimicrobial activity using agar well diffusion on solid media. The microorganisms used in the present study include Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella flexneri, Proteus vulgaris, and Candida albicans. Outcome: The study revealed that 50µl extract of Kukkiladhichoornam had significant inhibiting activity against Shigellaflexneri (17mm), Streptococcus pyogenes (17mm) and Candida albicans (18mm).

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Antimicrobial activity of Eucalyptus globulus oil, xylitol and papain: a pilot study

Revista da Escola de Enfermagem da USP ◽

10.1590/s0080-623420150000200005 ◽

2015 ◽

Vol 49 (2) ◽

pp. 0216-0220 ◽

Cited By ~ 3

Author(s):

Valéria de Siqueira Mota ◽

Ruth Natalia Teresa Turrini ◽

Vanessa de Brito Poveda

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Eucalyptus Globulus ◽

Antimicrobial Effect ◽

Inhibition Zone ◽

Proteus Vulgaris ◽

Test And Evaluation ◽

Agar Diffusion Test

OBJECTIVE To evaluate the in vitro antimicrobial activity of the Eucalyptus globulus essential oil, and of the xylitol and papain substances against the following microorganisms: Pseudomonas aeruginosa; Samonella sp.; Staphylococus aureus; Proteus vulgaris; Escherichia coli and Candida albicans. METHOD The in vitro antimicrobial evaluation was used by means of the agar diffusion test and evaluation of the inhibition zone diameter of the tested substances. Chlorhexidine 0.5% was used as control. RESULTS The Eucalyptus globulus oil showed higher inhibition than chlorhexidine when applied to Staphylococcus aureus, and equal inhibition when applied to the following microorganisms: Escherichia coli, Proteus vulgaris and Candida albicans. Papain 10% showed lower antimicrobial effect than chlorhexidine in relation to Candida albicans. Xylitol showed no inhibition of the tested microorganisms. CONCLUSION The Eucalyptus globulus oil has antimicrobial activity against different microorganisms and appears to be a viable alternative as germicidal agent hence, further investigation is recommended.

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Antimicrobial activity of grape fruit seed crude extract on microbial growth

Baghdad Science Journal ◽

10.21123/bsj.6.4.719-722 ◽

2009 ◽

Vol 6 (4) ◽

pp. 719-722

Author(s):

Baghdad Science Journal

Keyword(s):

Streptococcus Pyogenes ◽

Microbial Growth ◽

Inhibitory Concentration ◽

Ethanolic Extract ◽

Antimicrobial Effect ◽

Gram Negative Bacteria ◽

Gram Positive Bacteria ◽

Yeast Strains ◽

Grape Fruit

Antimicrobial and antiyeast activity of ethanolic and aqueous extract of grape fruit seed (Citrus paradise ; Rutaceaa) was examined against 10 bacterial and 2 yeast strains. The level of the antimicrobial effects was established using an in vitro agar assay and minimum inhibitory concentration (MIC). In general ethanolic extract were more effective on gram positive bacteria than gram negative bacteria and strongest antimicrobial effect against Streptococcus pyogenes and Salmonella entritidis. Other tested bacteria and yeasts were sensitive to extract ranging from 4 to 16 mg/ml and more.

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Comparative Antimicrobial Activity Study of Brassica oleceracea

Proceedings ◽

10.3390/ecsoc-22-05662 ◽

2018 ◽

Vol 9 (1) ◽

pp. 64 ◽

Cited By ~ 1

Author(s):

Sandeep Waghulde ◽

Nilofar Abid Khan ◽

Nilesh Gorde ◽

Mohan Kale ◽

Pravin Naik ◽

...

Keyword(s):

Antimicrobial Activity ◽

Streptococcus Pyogenes ◽

Antimicrobial Agents ◽

Antimicrobial Effect ◽

Significant Inhibition ◽

Phytochemical Analysis ◽

Gram Negative Bacteria ◽

Gram Positive Bacteria ◽

White Cabbage

Medicinal plants are in rich source of antimicrobial agents. The present study was carried out to evaluate the antimicrobial effect of plants from the same species as Brassica oleceracea namely, white cabbage and red cabbage. The preliminary phytochemical analysis was tested by using a different extract of these plants for the presence of various secondary metabolites like alkaloids, flavonoids, tannins, saponins, terpenoids, glycosides, steroids, carbohydrates, and amino acids. The in vitro antimicrobial activity was screened against clinical isolates viz gram positive bacteria Staphylococcus aureus, Streptococcus pyogenes, gram negative bacteria Escherichia coli, Pseudomonas aeruginosa. Extracts found significant inhibition against all the pathogens.

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PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEM OF THE HERB BASELLA ALBA L. VAR RUBRA (L.) STEWART (FAMILY-BASELLACEAE)

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017.v9i3.19599 ◽

2017 ◽

Vol 9 (3) ◽

pp. 91

Author(s):

Juman Deka ◽

Uttam Borah ◽

Biswajit Dash ◽

Suvakanta Dash ◽

Lawrence Kalita

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Ethanolic Extract ◽

Phytochemical Screening ◽

Bacterial Strains ◽

Bioactive Constituents ◽

E Coli ◽

Basella Alba ◽

Phytochemical Profile

Objective: To evaluate the antimicrobial activity ethanolic extracts of Basella alba L. var. rubra (L.) stewart (family-basellaceae) along with phytochemical analysis.Methods: The ethanolic extract of stems of the herb Basella alba L. var. rubra (L.) stewart (family-basellaceae) were prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts were examined against 2 bacterial strains among one is gram positive and other is gram negative and2 fungal strains using agar well diffusion method.Results: The present study describes the phytochemical profile and antimicrobial activity of Basella alba L. var. rubra (L.) stewart. Preliminary phytochemical screening of the extract were carried out and it revealed the presence of carbohydrates, tannins, terpene, saponin. The presence of these bioactive constituents is associated with the antimicrobial activity of the plant. The extract solvated by ethanol showed varying levels of antimicrobial activity against the tested bacteria namely E. coli, S. aureus, and fungi namely Candida albicans and Trichophyton rubrum. The study showed that the extract has a marked Sensitivity towards antibacterial strains namely E. coli, S. aureus, and antifungal strain namely Candida albicans and Trichophyton rubrum.Conclusion: The present study concluded ethanolic extract of stem of theBasella alba L. var. rubra (L.) stewartrevels many phytochemicals such as carbohydrate,tenin, saponin etc. This extract was found to possess promising antimicrobial activity when compared with the standards.

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