ANTIMICROBIAL ACTIVITY OF 3- (1- (3- HYDROXYPHENYL) AMINO) ETHYLIDENE) CHROMAN-2,4-DIONE AND ITS CORRESPONDING PALLADIUM(II) COMPLEX

2021 ◽  
Author(s):  
Edina Avdović ◽  
◽  
Žiko B. Milanović ◽  
Milanka Radulović ◽  
Dušan S. Dimić
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Coumarin Derivative ◽  
The Other ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Microdilution Method ◽  
Bacteria And Fungi

In this manuscript, the in vitro antimicrobial activity of the previously synthesized coumarin derivative 3- (1- (3-hydroxyphenyl) amino) ethylidene) chroman-2,4-dione (L) and its corresponding palladium (II) complex (C) were examined. Their antimicrobial activity was screened against four strains of bacteria Bacillus cereus (ATCC 11778) G+; Staphylococcus aureus (ATCC 13709) G+; Klebsiella pneumoniae (ATCC 27736) G-; and Escherichia coli (ATCC 2592) G-) and three strains of fungi (Aspergillus flavus (ATCC15517); Candida albicans (ATCC 10231); Fusarium oxysporum (ATCC 695) using disc diffusion and microdilution method. The obtained minimum inhibitory concentration (MIC) values by microdilution method for ligand and complex are similar for all tested bacteria and fungi, which means that both compounds have a similar antimicrobial effect. On the other hand, analysis of zone of inhibition (ZI) values for the tested compounds shows that the complex is generally somewhat more active than the ligand.

scholarly journals Stereospecific ligands and their complexes, Part VIII: Antimicrobial activity of palladium(II) complexes with 0,0'-dialkyl esters of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)-pentanoic acid

2012 ◽  
Vol 66 (3) ◽  
pp. 349-355 ◽  
Author(s):  
Dragoslav Ilic ◽  
Jelena Vujic ◽  
Ivana Radojevic ◽  
Olgica Stefanovic ◽  
Ljiljana Comic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
General Formula ◽  
Inhibitory Concentration ◽  
Pentanoic Acid ◽  
In Vitro Antimicrobial Activity ◽  
Microdilution Method ◽  
Vis Spectroscopy ◽  
Bacteria And Fungi

Palladium(II) complexes (1-4) of general formula [PdCl2(R2-S,S-eddp)] with bidentate N,N?-ligands, O,O'-dialkyl esters (R = ethyl, n-propyl, n-butyl and n-pentyl), of (S,S)-ethylenediamine-N,N?-di-2-(4-methyl)pentanoic acid (S,S-eddp) were prepared and characterized by microanalysis, infrared and UV/VIS spectroscopy. The ligands and its complexes were tested for their in vitro antimicrobial activity against 15 species of bacteria and fungi. Testing is performed by the microdilution method, with the minimum inhibitory concentration (MIC) and the minimum microbicidal concentration (MMC) being determined. The MIC values were in range from 4.9 ?g cm-3 to > 5000 ?g cm-3 while MMC values ranged from 78 ?g cm-3 to > 5000 ?g cm-3. Palladium(II) complexes [PdCl2(Ln)] (n = 1-4) have statistically significant higher activity than the corresponding ligands. The complex 4 displayed the strongest activity among all tested compounds.

Comparative studies of the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola on Klebsiella pneumonia and Candida albicans in vitro

2021 ◽  
Vol 16 (1) ◽  
pp. 52-58
Author(s):  
Elizabeth Winful ◽  
Olanikpekun Idowu ◽  
Opeoluwa O. Fasanya ◽  
Nkechi E. Egbe
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Garcinia Kola ◽  
Zones Of Inhibition

Antimicrobial resistance has become a global health problem. Although a wide range of chemotherapeutic antimicrobials are available for treatment of microbial related infections and diseases, development of resistance to these chemotherapeutic agents is rapidly on the increase. Extracts from some plants have shown some promise in antimicrobial activity. This has led to the screening of several medicinal plants for their potential antimicrobial activity. Therefore, this study was aimed at evaluating the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola against Klebsiella pneumonia and Candida albicans in vitro. The Garcinia kola seeds extracts were obtained using 70% ethanol and distilled water respectively. Phytochemical screening of Garcinia kola revealed the presence of various potent phytochemicals such as tannins, saponins, flavonoids, alkaloids and glycosides. Both extracts of the seeds were investigated for antimicrobial activity using disc diffusion and agar well diffusion sensitivity tests. The ethanol extract produced zones of inhibition of about 7.3 mm for Candida albicans only at a concentration of 800mg/ml for the disc diffusion test. For the agar well diffusion test, the aqueous extract produced zones of inhibition of about 9.5 mm, while the ethanol extract produced zone of inhibition of 19 mm against Candida albicans at a concentration of 800 mg/ml. However, at the same concentration, the ethanol extract produced zones of inhibition of about 8.5 mm against Klebsiella pneumoniae. The findings of this study revealed that Garcinia kola was not effective in treating Klebsiella pneumonia infections but has potential in treating Candida albicans infections. Keywords: Garcinia kola, Klebsiella pneumonia, Candida albicans

scholarly journals Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

2008 ◽  
Vol 25 (No. 2) ◽  
pp. 81-89 ◽  
Author(s):  
A. Adiguzel ◽  
H. Ozer ◽  
H. Kilic ◽  
B. Cetin
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Satureja Hortensis ◽  
Bacteria And Fungi

The present work reports the <i>in vitro</i> antimicrobial activities of the essential oil and methanol extract from <i>Satureja hortensis</i> as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), &gamma;-terpinene (18.56%), carvacrol (13.98%), and <i>p</i>-cymene (8.97). The essential oil of <i>Satureja hortensis</i> exhibited the activity against 25 bacteria, 8 fungi, and a yeast, <i>C. albicans</i>; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 &micro;l/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

scholarly journals Phytochemical characterization, anti-cancer and antimicrobial activity of isolated fractions of Alysicarpus vaginalis

2017 ◽  
Vol 12 (1) ◽  
pp. 77 ◽  
Author(s):  
Ganesh Tapadiya ◽  
Mayura A. Kale ◽  
Shweta Saboo
Keyword(s):  
Antimicrobial Activity ◽  
Cell Lines ◽  
Methanolic Extract ◽  
Hep G2 ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Anti Cancer ◽  
Proliferative Assay ◽  
Bacteria And Fungi

<p class="Abstract">The methanolic extract of <em>Alysicarpus </em>vaginalis was selected for fractionation due to its known reported biological activity. The four fractions were separated and subjected for<em> in vitro</em> antimitotic and anti-proliferative assays along with anti-cancer activity on two human cancers cell lines (SK-MEL-2 and Hep-G2). The antimicrobial potential of fractions had been evaluated against bacteria and fungi. From all fractions, acetone and n-butanol fractions were effective against the cell lines. They show strong inhibitory action with mitotic index 6.2 and 8.4 mg/mL and IC<sub>50 </sub>values of anti-proliferative assay in between 19.7 to 14.2 mg/mL respectively, which was found to be comparable to the standard methothrexate 5.9 mg/mL and 13.2 mg/mL respectively. In antimicrobial activity, the zone of inhibition had been observed in the range of 12-27 mm and MIC value was found in the range of 0.2-0.1 mg/mL. The acetone fraction was found to be most active against fungi, and<em> E. coli</em> whereas chloroform and n-butanol fractions were more effective against <em>S. aureus</em> and <em>B. </em>subtilis. The phytochemical characterization by HPLC analysis indicated the presence of important polyphenolic and steroidal compounds.</p>

scholarly journals In VitroAntimicrobial Activity of Essential Oil ofThymus schimperi,Matricaria chamomilla,Eucalyptus globulus, andRosmarinus officinalis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Awol Mekonnen ◽  
Berhanu Yitayew ◽  
Alemnesh Tesema ◽  
Solomon Taddese
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Drug Preparation ◽  
Bacteria And Fungi

In this study, thein vitroantimicrobial activities of four plant essential oils (T. schimperi,E. globulus,R. officinalis, andM. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils ofT. schimperi,E. globulus, andR. officinaliswere active against bacteria and some fungi. The antimicrobial effect ofM. chamomillawas found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values ofT. schimperiwere<15.75 mg/mL for most of the bacteria and fungi used in this study. The minimum inhibitory concentration values of the other essential oils were in the range of 15.75–36.33 mg/mL against tested bacteria. This study highlighted the antimicrobial activity of the essential oil ofE. globulus,M. chamomilla,T. Schimperi, andR. officinalis. The results indicated thatT. schimperihave shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

scholarly journals In vitro evaluation of the antimicrobial activity of Basil (Ocimum baslicum L.) and Coriander (Coriandrum satirum L.) oil extracts on Streptococus mutans

2018 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Mariana Lourenço ◽  
Adilson Cesar Abreu Bernardi ◽  
Nadia Lunardi ◽  
Rodolfo Jorge Boeck Neto ◽  
Patricia Sigilló Mazzoni Bernardi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Growth Control ◽  
Ocimum Basilicum ◽  
Coriandrum Sativum ◽  
Microdilution Method

Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

scholarly journals The In Vitro Antimicrobial Effects of Lavandula angustifolia Essential Oil in Combination with Conventional Antimicrobial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Stephanie de Rapper ◽  
Alvaro Viljoen ◽  
Sandy van Vuuren
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Lavandula Angustifolia ◽  
Gram Negative ◽  
Stock Solutions

The paper focuses on the in vitro antimicrobial activity of Lavandula angustifolia Mill. (lavender) essential oil in combination with four commercial antimicrobial agents. Stock solutions of chloramphenicol, ciprofloxacin, nystatin, and fusidic acid were tested in combination with L. angustifolia essential oil. The antimicrobial activities of the combinations were investigated against the Gram-positive bacterial strain Staphylococcus aureus (ATCC 6538) and Gram-negative Pseudomonas aeruginosa (ATCC 27858) and Candida albicans (ATCC 10231) was selected to represent the yeasts. The antimicrobial effect was performed using the minimum inhibitory concentration (MIC) microdilution assay. Isobolograms were constructed for varying ratios. The most prominent interaction was noted when L. angustifolia essential oil was combined with chloramphenicol and tested against the pathogen P. aeruginosa (ΣFIC of 0.29). Lavendula angustifolia essential oil was shown in most cases to interact synergistically with conventional antimicrobials when combined in ratios where higher volumes of L. angustifolia essential oil were incorporated into the combination.

scholarly journals Antimicrobial and Antihypercholesterolemic Activities of Pulicaria gnaphalodes

Dose-Response ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 155932582090485 ◽  
Author(s):  
Syed Ali Raza Naqvi ◽  
Syed Muhammad Ali Shah ◽  
Laiba Kanwal ◽  
Muhammad Saeed ◽  
Atta-ul-Haq ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacterial Infections ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Ethanol Extract ◽  
Alternative Therapies ◽  
Zone Of Inhibition

Multidrug resistance has increased globally in the communities. Bacterial infections associated with health care have weakened the existing antimicrobial therapy and demand the search for alternative therapies. In the present investigation, the medicinal plant Pulicaria gnaphalodes from Quetta, Pakistan, has been screened for antimicrobial potential. In vitro antimicrobial efficacy of P gnaphalodes extracts (methanol and ethanol) was quantitatively evaluated on the basis of zone of inhibition against different bacteria and minimum inhibitory concentration (MIC). In vivo, antihypercholesterolemic activity is determined in different rat groups. The results of the study indicated that the ethanol extract of P gnaphalodes showed maximum zone of inhibition for Bacillus subtilis of 12.1 ± 1.1 mm from all others. The methanol extract showed maximum zone of inhibition for Staphylococcus aureus of 11.9 ± 1.0 mm and rifampicin showed maximum zone of inhibition of 23.1 ± 0.9 mm. The results of ethanol and methanol extract of P gnaphalodes against different bacteria revealed that this plant has greater antimicrobial activity. However, the plant extract shows nonsignificant antihypercholesterolemic activity. The extract of this plant can be utilized as medicine to inhibit several infections caused by some bacterial pathogens found in human body.

Crystal structure and in vitro antimicrobial activity studies of Robustic acid and other Alpinumisoflavones isolated from Millettia thonningii

2019 ◽  
Vol 234 (4) ◽  
pp. 229-235
Author(s):  
Jerry Joe E.K. Harrison ◽  
Moscoh Daniel Ayine-Tora ◽  
Benjamin Appiagyei ◽  
Felix C. Mills-Robertson ◽  
William A. Asomaning ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Inhibitory Concentration ◽  
Salmonella Typhi ◽  
The Other ◽  
Chemical Probes ◽  
Zone Of Inhibition ◽  
Topological Information ◽  
Inhibitory Potential ◽  
Coumarin Ring

Abstract We report a systematic study of the antibacterial inhibitory potential of isoflavone natural products isolated from the seeds extracts of Millettia thonningii. In an effort to gain bond topological information which may have consequences for the observed bioactivities, the crystal structure of robustic acid was solved and refined using the independent atom as well as the invariom model, and the structures were compared. Robustic acid contains a fused tricyclic unit with a benzopyran moiety, with a phenylene ring substitution on the coumarin ring similar to the alpinumisoflavones isolated from this plant. At a minimum inhibitory concentration of ~1 mg/mL, alpinumisoflavone and robustic acid were found to be cytotoxic to Staphylococcus aureus (ATCC 25923) showing a zone of inhibition (ZOI) of ~9 mm. On the other hand, at ~2 mg/mL, these compounds were found to be bacteriostatic to a hospital isolate of Salmonella typhi with about 7 mm ZOI. Taken together, these compounds offer potential new avenues for targeting both Gram positive and negative bacteria and could be useful as chemical probes for understanding these pathogens in an effort to overcome drug resistance.

scholarly journals Comparison of antimicrobial activity between intraoperatively soaked bacitracin sutures and triclosan coated suture

2020 ◽  
Author(s):  
Shane T. Musick ◽  
Jeremy M. Adkins ◽  
Roy Al-Ahmar ◽  
Hongwei D. Yu ◽  
Anthony M. Alberico
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antimicrobial Effect ◽  
Zone Of Inhibition ◽  
Experimental Procedure ◽  
Procedural Change ◽  
Test Organisms ◽  
Irrigation Solution

AbstractWith the easily available option for surgeons to soak their suture in anti-biotic irrigating solution intraoperatively in mind, this study was designed to evaluate the ability of suture soaked in bacitracin irrigating solution to inhibit the growth of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. Using standard experimental procedure, sterile suture was soaked in Bacitracin suture, and dried for 10 minutes or 6 hours, incubated for 24 h on inoculated plates, and examined for zone of inhibition around the suture. This was compared to control unsoaked suture and antimicrobial suture (AMS) currently on the market to determine if the minor intra operative procedural change of placing suture in antibiotic irrigation solution instead of on the sterile table could confer some antimicrobial activity. The study found the Bacitracin soaked suture (BSS) consistently inhibited the growth of the test organisms. For both organisms, the BSS exhibited a significantly larger zone of inhibition compared to the unsoaked control suture. However, the AMS currently on the market exhibited a larger zone of inhibition compared to the BSS. Placing sutures in a bacitracin irrigation solution intraoperatively instead of directly on the sterile table can achieve some of the in vitro antimicrobial effect seen from AMS currently on the market. This may result in reduced rates of SSIs and associated costs without major procedural change and at reduced overhead.

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