Cytotoxic Potential of Fertility Booster Medicinal Plants as Evident through Brine Shrimp Toxicity Assay

Medicinal plants are a good source of novel therapeutic drugs, due to the phytochemicals present. Artemia, commonly known as brine shrimp, is a tiny halophilic invertebrate belonging to class Crustacean, which plays an important role in saline aquatic and marine eco-systems. Besides its usage in aquaculture, it is also highly valued for its application in toxicity detection and it is used in areas such as Ecology, Physiology, Ecotoxicology, Aquaculture and Genetics. Furthermore, Artemia based lethality assay (brine shrimp lethality assay, BSLA) is rapid, convenient and low cost. Presently, brine shrimp lethality assays are enormously employed in research and applied toxicology. It has been used in the study of natural products as a preliminary toxicity assay to screen a large number of extracts and compounds for drug discovery in medicinal plants. The aim of this review paper is to collect, organize, select and discuss the existing knowledge about the different uses of Artemia salina as a bench-top bioassay for the discovery and purification of bioactive natural products.

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Green-Synthesized FeSO4 Nanoparticles Exhibit Antibacterial and Cytotoxic Activity by DNA Degradation

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200101111643 ◽

2020 ◽

Vol 21 (7) ◽

pp. 587-595

Author(s):

B. Bharath ◽

Santanu Sasidharan ◽

Sai K. Bhamidipati ◽

Prakash Saudagar

Keyword(s):

Brine Shrimp ◽

Pathogenic Bacteria ◽

Dna Degradation ◽

Ftir Analysis ◽

Toxicity Assay ◽

One Pot ◽

Mechanism Of Toxicity ◽

Potential Applications ◽

Gel Shift Assay ◽

Brine Shrimp Toxicity

Objective: The current study reports a green, rapid and one-pot synthesis of FeSO4 nanoparticles using Hibiscus rosasinensis floral extract as a reducing and capping agent. 0.5M of FeSO4 was stirred with the floral extract of H. rosasinensis for around 20 minutes at 37ºC and pH 7. Methods: The development of pink color was considered as the endpoint of reduction and the nanoparticles were characterized by UV-Vis spectrum, EDAX, DLS, FTIR, FESEM, and XRD. UV-Vis spectral analysis indicated a peak at 530 nm and EDAX measurement revealed the presence of Fe, S, O and C elements in the nanoparticle sample. The FTIR analysis showed amines, alcohol and alkene groups that act as capping agents for the produced nanoparticles. FESEM and XRD determination presented FeSO4 nanoparticles of 40-60 nm in size. The synthesized nanoparticles were found to have antibacterial activity against 6 pathogenic bacteria with MIC and MBC of 40 mg/mL. Results: To determine the toxicity at the eukaryotic level, brine shrimp toxicity assay was conducted and 100% mortality was found at concentrations >0.06 mg/mL. Gel shift assay suggested the mechanism of toxicity of FeSO4 NPs by binding and degradation of DNA molecules. Conclusion: From the results, the authors demonstrate the ease of green synthesis of FeSO4 nanoparticles and its bioactivity that may have potential applications as drugs and drug delivery systems against various diseases.

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Mangrove Tannin (Rhizophora apiculata) Complexes with Copper (II) Ion as an Antifoulant in Antifouling Paint for Fish Net

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1043.204 ◽

2014 ◽

Vol 1043 ◽

pp. 204-208 ◽

Cited By ~ 2

Author(s):

Azraa Achmad ◽

Jain Kassim ◽

Azwin Usol Ghafli ◽

Hairul Hamdan

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Fourier Transform ◽

Brine Shrimp ◽

Solution Temperature ◽

Toxicity Assay ◽

X Ray ◽

Rhizophora Apiculata ◽

Brine Shrimp Toxicity ◽

Scanning Electron

Mangrove tannin was used to be complex with copper (II) ion. The optimum condition of copper tannate complex was studied using four parameters which were copper dosage, pH solution, temperature and time. The copper tannate complex was characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDX) and brine shrimp toxicity assay. This study suggested that copper tannate complex has a great potential to be develop as an alternative antifoulant.

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Antioxidant, Antimicrobial and Cytotoxic Activities of Samanea saman (Jacq.) Merr.

Stamford Journal of Pharmaceutical Sciences ◽

10.3329/sjps.v3i1.6792 ◽

1970 ◽

Vol 3 (1) ◽

pp. 11-17 ◽

Cited By ~ 1

Author(s):

Afia Ferdous ◽

Mohammad Zafar Imam ◽

Tajnin Ahmed

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Carbon Tetrachloride ◽

Brine Shrimp ◽

Dpph Radical ◽

Cytotoxic Potential ◽

Ic50 Value ◽

Total Antioxidant ◽

Samanea Saman ◽

Soluble Fractions

In the present investigation the n-hexane, carbon tetrachloride and choloroform soluble fractions of crude methanolic extract of Samanea saman bark were tested for antioxidant, antimicrobial and cytotoxic potential. Antioxidant activity of the extracts was evaluated by DPPH radical scavenging assay and total antioxidant activity test. Antimicrobial activity was tested using disc diffusion method against thirteen bacteria and three fungi and cytotoxicity was tested by brine shrimp lethality bioassay. Chloroform and hexane soluble fraction showed IC50 value of 12μg/ml and 14μg/ml respectively in scavenging DPPH radical while the reference Butylated hydroxytoluene showed an IC50 value of 10μg/ml. The carbon tetrachloride fraction showed the highest total antioxidant capacity. The carbon tetrachloride fraction was also found to possess mild to moderate microbial growth inhibitory capacity. In the brine shrimp lethality bioassay, the n-hexane, carbon tetrachloride, chloroform soluble fractions showed LC50 value of 14.94μg/ml, 0.831μg/ml and 3.288μg/ml respectively. The results suggest good antioxidant and cytotoxic potential of chloroform and hexane soluble fractions and antimicrobial activity of carbon tetrachloride fraction of Samanea saman bark extract. Key Words: Samanea saman; Leguminoseae; Cytotoxicity; Antimicrobial; Antioxidant; Total antioxidant capacity. DOI: 10.3329/sjps.v3i1.6792S. J. Pharm. Sci. 3(1): 11-17

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Study on Phytochemical, Antibacterial, Antioxidant and Toxicity Profile of Viscum album Linn Associated with Acacia catechu

Nepal Journal of Biotechnology ◽

10.3126/njb.v3i1.14234 ◽

2015 ◽

Vol 3 (1) ◽

pp. 60-65

Author(s):

Meena Kusi ◽

Kanti Shrestha ◽

Rajani Malla

Keyword(s):

Brine Shrimp ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Viscum Album ◽

Biologically Active ◽

Frap Assay ◽

Antioxidant Power ◽

Percolation Method ◽

Brine Shrimp Toxicity ◽

Acacia Catechu

This study focuses on antibacterial, antioxidant and toxic potentials of Viscum album Linn, commonly known as European mistletoe associated with Acacia catechu (Khayer in Nepali). Methanol extract of the aerial parts of the Mistletoe was prepared by cold percolation method. The resulting extract was simultaneously subjected to phytochemical screening; anti-microbial activity; anti-oxidant potential and Brine shrimp toxicity test. The major biologically active phyto-constituents observed were alkaloids, glycosides, saponins, polyphenols, flavonoids, tannins, terpenoids and cardiac glycosides. Upon antibacterial activity screening, the plant extract was found to be highly effective against Pseudomonas aeruginosa with the zone of inhibition 16±1mm compared to 17±1mm of chloramphenicol (50 mcg). The antioxidant activity as EC50 value by DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity was found to be 1.58 mg/ml while the ferric reducing capacity was measured to be 282.83±19.55 mg FeSO4.7H2O eqvt/g dry wt. of the extract during Ferric Ion Reducing Antioxidant Power (FRAP) Assay. The LC50 value for Brine Shrimp Toxicity Assay was found to be 31.62 ppm. This study shows the medicinal value of the mistletoe associated with Acacia catechu. Further meticulous analysis of this plant might lead to identification of active biomolecules effective as drugs for various ailmentsNepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 60-65

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