Green-Synthesized FeSO4 Nanoparticles Exhibit Antibacterial and Cytotoxic Activity by DNA Degradation

2020 ◽  
Vol 21 (7) ◽  
pp. 587-595
Author(s):  
B. Bharath ◽  
Santanu Sasidharan ◽  
Sai K. Bhamidipati ◽  
Prakash Saudagar
Keyword(s):  
Brine Shrimp ◽  
Pathogenic Bacteria ◽  
Dna Degradation ◽  
Ftir Analysis ◽  
Toxicity Assay ◽  
One Pot ◽  
Mechanism Of Toxicity ◽  
Potential Applications ◽  
Gel Shift Assay ◽  
Brine Shrimp Toxicity

Objective: The current study reports a green, rapid and one-pot synthesis of FeSO4 nanoparticles using Hibiscus rosasinensis floral extract as a reducing and capping agent. 0.5M of FeSO4 was stirred with the floral extract of H. rosasinensis for around 20 minutes at 37ºC and pH 7. Methods: The development of pink color was considered as the endpoint of reduction and the nanoparticles were characterized by UV-Vis spectrum, EDAX, DLS, FTIR, FESEM, and XRD. UV-Vis spectral analysis indicated a peak at 530 nm and EDAX measurement revealed the presence of Fe, S, O and C elements in the nanoparticle sample. The FTIR analysis showed amines, alcohol and alkene groups that act as capping agents for the produced nanoparticles. FESEM and XRD determination presented FeSO4 nanoparticles of 40-60 nm in size. The synthesized nanoparticles were found to have antibacterial activity against 6 pathogenic bacteria with MIC and MBC of 40 mg/mL. Results: To determine the toxicity at the eukaryotic level, brine shrimp toxicity assay was conducted and 100% mortality was found at concentrations >0.06 mg/mL. Gel shift assay suggested the mechanism of toxicity of FeSO4 NPs by binding and degradation of DNA molecules. Conclusion: From the results, the authors demonstrate the ease of green synthesis of FeSO4 nanoparticles and its bioactivity that may have potential applications as drugs and drug delivery systems against various diseases.

Mangrove Tannin (Rhizophora apiculata) Complexes with Copper (II) Ion as an Antifoulant in Antifouling Paint for Fish Net

2014 ◽  
Vol 1043 ◽  
pp. 204-208 ◽  
Author(s):  
Azraa Achmad ◽  
Jain Kassim ◽  
Azwin Usol Ghafli ◽  
Hairul Hamdan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fourier Transform ◽  
Brine Shrimp ◽  
Solution Temperature ◽  
Toxicity Assay ◽  
X Ray ◽  
Rhizophora Apiculata ◽  
Brine Shrimp Toxicity ◽  
Scanning Electron

Mangrove tannin was used to be complex with copper (II) ion. The optimum condition of copper tannate complex was studied using four parameters which were copper dosage, pH solution, temperature and time. The copper tannate complex was characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDX) and brine shrimp toxicity assay. This study suggested that copper tannate complex has a great potential to be develop as an alternative antifoulant.

scholarly journals Study on Phytochemical, Antibacterial, Antioxidant and Toxicity Profile of Viscum album Linn Associated with Acacia catechu

2015 ◽  
Vol 3 (1) ◽  
pp. 60-65
Author(s):  
Meena Kusi ◽  
Kanti Shrestha ◽  
Rajani Malla
Keyword(s):  
Brine Shrimp ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Viscum Album ◽  
Biologically Active ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Percolation Method ◽  
Brine Shrimp Toxicity ◽  
Acacia Catechu

This study focuses on antibacterial, antioxidant and toxic potentials of Viscum album Linn, commonly known as European mistletoe associated with Acacia catechu (Khayer in Nepali). Methanol extract of the aerial parts of the Mistletoe was prepared by cold percolation method. The resulting extract was simultaneously subjected to phytochemical screening; anti-microbial activity; anti-oxidant potential and Brine shrimp toxicity test. The major biologically active phyto-constituents observed were alkaloids, glycosides, saponins, polyphenols, flavonoids, tannins, terpenoids and cardiac glycosides. Upon antibacterial activity screening, the plant extract was found to be highly effective against Pseudomonas aeruginosa with the zone of inhibition 16±1mm compared to 17±1mm of chloramphenicol (50 mcg). The antioxidant activity as EC50 value by DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity was found to be 1.58 mg/ml while the ferric reducing capacity was measured to be 282.83±19.55 mg FeSO4.7H2O eqvt/g dry wt. of the extract during Ferric Ion Reducing Antioxidant Power (FRAP) Assay. The LC50 value for Brine Shrimp Toxicity Assay was found to be 31.62 ppm. This study shows the medicinal value of the mistletoe associated with Acacia catechu. Further meticulous analysis of this plant might lead to identification of active biomolecules effective as drugs for various ailmentsNepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 60-65

scholarly journals Biosynthesis and Potential Applications of Silver and Gold Nanoparticles and Their Chitosan-Based Nanocomposites in Nanomedicine

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Haliza Katas ◽  
Noor Zianah Moden ◽  
Chei Sin Lim ◽  
Terence Celesistinus ◽  
Jie Yee Chan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Metallic Nanoparticles ◽  
Cost Effective ◽  
One Pot ◽  
Silver And Gold Nanoparticles ◽  
Stabilizing Agents ◽  
Cost Effective Method ◽  
Multistep Process ◽  
Potential Applications ◽  
Biological Sources

Biosynthesized or biogenic metallic nanoparticles, particularly silver and gold nanoparticles (AgNPs and AuNPs, respectively), have been increasingly used because of their advantages, including high stability and loading capacity; moreover, these nanoparticles are synthesized using a green and cost-effective method. Previous studies have investigated reducing and/or stabilizing agents from various biological sources, including plants, microorganisms, and marine-derived products, using either a one-pot or a multistep process at different conditions. In addition, extensive studies have been performed to determine the biological or pharmacological effects of these nanoparticles, such as antimicrobial, antitumor, anti-inflammatory, and antioxidant effects. In the recent years, chitosan, a natural cationic polysaccharide, has been increasingly investigated as a reducing and/or stabilizing agent in the synthesis of biogenic metallic nanoparticles with potential applications in nanomedicine. Here, we have reviewed the mechanism of biosynthesis and potential applications of AgNPs and AuNPs and their chitosan-mediated nanocomposites in nanomedicine.

Brine Shrimp Toxicity of Leaf and Seed Extracts of Cassia alata Linn. and Their Antibacterial Potency

2004 ◽  
Vol 4 (3) ◽  
pp. 188-193 ◽  
Author(s):  
M.A. Awal ◽  
Ainun Nahar . ◽  
M. Shamim Hossain . ◽  
M.A. Bari . ◽  
M. Rahman . ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Cassia Alata ◽  
Brine Shrimp Toxicity ◽  
Seed Extracts

scholarly journals Screening of Plants Used as Traditional Anticancer Remedies in Mkuranga and Same Districts, Tanzania, Using Brine Shrimp Toxicity Bioassay

2018 ◽  
Vol 2018 ◽  
pp. 1-16 ◽  
Author(s):  
Daniel Z. Matata ◽  
Olipa D. Ngassapa ◽  
Francis Machumi ◽  
Mainen J. Moshi
Keyword(s):  
Plant Species ◽  
Brine Shrimp ◽  
Health Care Services ◽  
Literature Search ◽  
Strong Support ◽  
Preliminary Evaluation ◽  
Contributing Factors ◽  
Anticancer Compounds ◽  
Care Services ◽  
Brine Shrimp Toxicity

Background.Inadequate specialized cancer hospitals and high costs are contributing factors that delay cancer patients from accessing health care services in Tanzania. Consequently, majority of patients are first seen by Traditional Health Practitioners (THPs) before they access specialized services. This study presents ethnomedical information and preliminary evaluation of 25 plant species claimed by THPs in Mkuranga and Same districts of Tanzania on use for treatment of cancer. Literature search and laboratory investigation results are presented to support evaluation.Methods. This study was a single disease ethnomedical enquiry focusing on plants being used for cancer treatment.Face-to-face interviews and questionnaires were administered toeight (8) THPsin Mkuranga and Same districts on the claimed plants and their use for management of cancer. Plants were selected based on being frequently mentioned and emphasis given by THPs. Literature search and brine shrimp toxicity (BST) of methanol : dichloromethane (1:1) extracts was used as surrogates to evaluate strength of the claims.Results.This study reports 25 plant species used by the THPs in two districts of Tanzania. Eight plants (32%) have been reported in the literature to have activity against cancer cells. BST results revealed, 14 (56%) plants exhibited high toxicity against brine shrimps. The most active plants includedCroton pseudopulchellusPax (LC504.2μg/ml),Dalbergia melanoxylonGuill. & Perr. (LC506.8μg/ml),Loranthus micranthusLinn (LC504.0μg/ml),Ochna mossambicensisKlotzsch (LC503.3μg/ml), andSpirostachys africanaSond. (LC504.4μg/ml); their toxicity was comparable to that ofCatharanthus roseus(L) G. Don. (LC506.7μg/ml), an established source of anticancer compounds. Nine other plants had LC50values between (19.8 and 71.6)μg/ml, indicating also potential to yield anticancer.Conclusion.Literature search and BST results provide a strong support of the potential of the claimed plants to yield active anticancer compounds.

scholarly journals Synthesis of Carbon Quantum Dot Nanoparticles Derived from Byproducts in Bio-Refinery Process for Cell Imaging and In Vivo Bioimaging

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 387 ◽  
Author(s):  
Caoxing Huang ◽  
Huiling Dong ◽  
Yan Su ◽  
Yan Wu ◽  
Robert Narron ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Cell Imaging ◽  
Degradation Products ◽  
Green Fluorescence ◽  
One Pot ◽  
Carbon Nanoparticle ◽  
Carbon Quantum Dot ◽  
Potential Applications ◽  
Main Components

The carbon quantum dot (CQD), a fluorescent carbon nanoparticle, has attracted considerable interest due to its photoluminescent property and promising applications in cell imaging and bioimaging. In this work, biocompatible, photostable, and sustainably sourced CQDs were synthesized from byproducts derived from a biorefinery process using one-pot hydrothermal treatment. The main components of byproducts were the degradation products (autohydrolyzate) of biomass pretreated by autohydrolysis. The as-synthesized CQDs had a size distribution from 2.0–6.0 nm and had high percentage of sp2 and sp3 carbon groups. The CQDs showed blue-green fluorescence with a quantum yield of ~13%, and the fluorescence behaviors were found to be stable with strong resistance to photobleaching and temperature change. In addition, it is found that the as-synthesized CQDs could be used for imaging of cells and tumors, which show potential applications in bioimaging and related fields such as phototherapy and imaging.

scholarly journals Synthesis, Characterization and in vitro Antimicrobial Screening of Some Novel Series of 2-Azetidinone Derivatives Integrated with Quinoline, Pyrazole and Benzofuran Moieties

2020 ◽  
Vol 32 (4) ◽  
pp. 896-900
Author(s):  
M. Idrees ◽  
Y.G. Bodkhe ◽  
N.J. Siddiqui ◽  
S.S. Kola
Keyword(s):  
Mass Spectra ◽  
Pathogenic Bacteria ◽  
Catalytic Amount ◽  
Spectral Studies ◽  
One Pot ◽  
E Coli ◽  
One Pot Condensation ◽  
Cyclocondensation Reaction ◽  
In Vitro Antibacterial Activity

A series of 5-(benzofuran-2-yl)-N-(3-chloro-4-(2-(p-tolyloxy) substituted quinolin-3-yl)-2-oxoazetidin-1-yl)-1-phenyl-1H-pyrazole-3-carboxamide derivatives (4a-f) were synthesized with excellent yields by cyclocondensation reaction of 5-(benzofuran-2-yl)-N′-(2-(p-tolyloxy) substituted quinolin-3-yl)methylene)-1-phenyl-1H-pyrazole-3-carbohydrazide (3a-f) with chloroacetyl chloride in presence of triethylamine in DMF. One pot condensation of 5-(benzofuran-2-yl)-1-phenyl-1H-pyrazole-3-carbohydrazide (1) with 2-(p-tolyloxy) substituted quinoline-3-carbaldehyde (2a-f) in ethanol solvent in presence of catalytic amount of acetic acid gave intermediate compounds (3a-f). The structures of newly synthesized compounds have been substantiated through elemental analysis and spectral studies viz. 1H NMR, 13C NMR, IR and mass spectra. All the synthesized compounds were screened for their in vitro antibacterial activity against pathogenic bacteria such as S. aureus and E. coli at different concentrations.

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