Association Analysis between Exon II of GH Gene and Growth Traits in Sheep

Copy number variation (CNV) is a type of genomic variation with an important effect on animal phenotype. We found that the PIGY gene contains a 3600 bp copy number variation (CNV) region located in chromosome 6 of sheep (Oar_v4.0 36,121,601–36,125,200 bp). This region overlaps with multiple quantitative trait loci related to phenotypes like muscle density and carcass weight. Therefore, in this study, the copy number variation of the PIGY gene was screened in three Chinese sheep breeds, namely, Chaka sheep (CKS, May of 2018, Wulan County, Qinghai Province, China), Hu sheep (HS, May of 2015, Mengjin County, Henan Province, China), and small-tailed Han sheep (STHS, May of 2016, Yongjing, Gansu Province, China). Association analyses were performed on the presence of CNV and sheep body size traits. We used real-time quantitative PCR (qPCR) to detect the CNV for association analysis. According to the results, the loss-type CNV was more common than other types in the three breeds (global average: loss = 61.5%, normal = 17.5%, and gain = 21.0%). The association analysis also showed significant effects of the PIGY gene CNV on body weight, chest circumference, and circumference of the cannon bone of sheep. Sheep with gain-type CNV had better growth traits than those with other types. The results indicate a clear relationship between the PIGY gene CNV and growth traits of sheep, suggesting the use of CNV as a new molecular breeding marker.

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Differential Expression of KCNJ12 Gene and Association Analysis of Its Missense Mutation with Growth Traits in Chinese Cattle

Animals ◽

10.3390/ani9050273 ◽

2019 ◽

Vol 9 (5) ◽

pp. 273 ◽

Cited By ~ 1

Author(s):

Jie Cheng ◽

Wenwen Peng ◽

Xiukai Cao ◽

Yongzhen Huang ◽

Xianyong Lan ◽

...

Keyword(s):

Skeletal Muscle ◽

Missense Mutation ◽

Association Analysis ◽

Growth Traits ◽

Muscle Cells ◽

Skeletal Muscle Cells ◽

Economic Traits ◽

Functional Candidate Gene ◽

Chinese Cattle ◽

Bovine Skeletal Muscle

The potassium inwardly rectifying channel, subfamily J, member 12 (KCNJ12) gene is a promising candidate for economic traits because of its crucial roles in myoblast development. Here, a missense mutation (Cys > Arg) was first detected to be located in exon 3 of KCNJ12 from three Chinese cattle breeds by DNA-pool sequencing. Then, we performed an association analysis of this single-nucleotide polymorphism (SNP) with stature in three Chinese cattle populations (n = 820). A significantly positive correlation was revealed by a reduced animal general linear model and the CC genotype was the most favorable in three breeds. Further, we measured the expression profile of the KCNJ12 gene in various cattle tissues and primary bovine skeletal muscle cells. Ubiquitous expression with high abundance in muscle was observed. Further, in primary bovine skeletal muscle cells, the KCNJ12 mRNA expression was gradually up-regulated in differentiation medium (DM) compared with that in growth medium (GM), suggesting that the KCNJ12 gene is involved in bovine myocyte differentiation. Conclusively, the KCNJ12 gene is a functional candidate gene which can be used as a molecular marker for cattle breeding.

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Association analysis of bovine Foxa2 gene single sequence variant and haplotype combinations with growth traits in Chinese cattle

Gene ◽

10.1016/j.gene.2013.11.046 ◽

2014 ◽

Vol 536 (2) ◽

pp. 385-392 ◽

Cited By ~ 8

Author(s):

Mei Liu ◽

Mijie Li ◽

Shaoqiang Wang ◽

Yao Xu ◽

Xianyong Lan ◽

...

Keyword(s):

Association Analysis ◽

Growth Traits ◽

Sequence Variant ◽

Chinese Cattle ◽

Single Sequence

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Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta

Gene ◽

10.1016/j.gene.2014.11.020 ◽

2015 ◽

Vol 555 (2) ◽

pp. 297-304 ◽

Cited By ~ 15

Author(s):

Donghong Niu ◽

Lie Wang ◽

Zhiyi Bai ◽

Shumei Xie ◽

Honggang Zhao ◽

...

Keyword(s):

Association Analysis ◽

Growth Traits ◽

Sinonovacula Constricta ◽

Razor Clam ◽

Mstn Gene ◽

Expression Characterization

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RELATIONSHIP OF GROWTH HORMONE GENE WITH SOME OF PRODUCTIVE TRAITS OF COMMON CARP Cyprinus carpio.L

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v49i6.137 ◽

2018 ◽

Vol 49 (6) ◽

Author(s):

AL-Azzawy & Al-Khshali

Keyword(s):

Growth Rate ◽

Common Carp ◽

Growth Traits ◽

Direct Sequencing ◽

Growth Hormone Gene ◽

Daily Growth ◽

Total Weight Gain ◽

Gh Gene ◽

Productive Traits ◽

Relationship Of

This study was carried out at Al-Radhwaniyah fish Reservoir (Baghdad) to investigate the polymorphism of GH gene and relationship with some of productive characteristics (total weight gain (T.W.G), daily growth rate (D.G.R), relative growth rate (R.G.R) and specific growth rate (S.G.R) in common carp. single nucleotide polymorphism (SNPs) in GH gene was analyzed by direct sequencing. Two SNP were identified in the third intron of GH1, the first SNP at site A1132T was negative correlated with growth traits, AA Genotype (wild) was significant(p<0.05) correlated with growth traits. The second SNP was happened at site of G1217T, any genotype significantly does not correlated with growth traits. the study summarized that identification of SNPs associated with growth performance can be candidate as genetics markers in marker-assisted selection (MAS) programs for improving growth traits in common carp.

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Polymorphism Exploration of Growth Family (GH, GHRH and PIT-1) Genes Polymorphisms of Local Swamp Buffalo for Productivity Improvement in North Tapanuli Regency, North Sumatra

BIO Web of Conferences ◽

10.1051/bioconf/20213302005 ◽

2021 ◽

Vol 33 ◽

pp. 02005

Author(s):

Anneke Anggraeni ◽

Chalid Thalib ◽

Angga Ardhati Rani Hapsari

Keyword(s):

Growth Traits ◽

Restriction Enzymes ◽

Economic Traits ◽

Productivity Improvement ◽

Pcr Rflp ◽

Gh Gene ◽

Molecular Selection ◽

Swamp Buffalo ◽

Livestock Productivity ◽

North Sumatra

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. Genetic polymorphism of the growth family (GH, GHRH, and PIT1) genes were studied in local swamp buffalo (106 hds.) from a government buffalo breeding station (46 heads) and smallholders (60 heads) in North Tapanuli District, North Sumatra Province. Genotype variants of the three genes were identified by PCR-RFLP method using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene) and HinfI (PIT -1 gene). Genotyping on individual GH_g.1547T>C, GHRH_g.4666G>C, and PIT -1_g.1256G >A loci resulted only one type genotype, respectively TT, CC, an d AA, with one type of allele, respectively T, C, and A. Heterozygosity observation (Ho) and expectation (He) values values and the PIC value for each locus was 0.00. It could be suggested to increase genotype frequenciest of the three growth genes that are positively associated with the growth traits and economic traits of the buffalo.

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Expression profiles and polymorphic identification of the <i>ACSL1</i> gene and their association with body size traits in Dezhou donkeys

Archives Animal Breeding ◽

10.5194/aab-63-377-2020 ◽

2020 ◽

Vol 63 (2) ◽

pp. 377-386

Author(s):

Zhenyu Lai ◽

Fei Wu ◽

Zihui Zhou ◽

Mei Li ◽

Yuan Gao ◽

...

Keyword(s):

Molecular Markers ◽

Body Size ◽

Association Analysis ◽

Growth And Development ◽

Growth Traits ◽

Expression Profiles ◽

Wild Genotype ◽

Mutant Genotype ◽

Polymorphic Loci ◽

Genotype Ii

Abstract. Finding out the genetic mechanism of growth and development traits and the development of related molecular markers can help improve the breeding of livestock. The long-chain acyl coenzyme A synthase 1 (ACSL1) gene plays a major role in lipid synthesis and fatty acid catabolism. However, there are few studies on the ACSL1 gene polymorphism of Dezhou donkeys. This study analyzed the expression level of the ACSL1 gene in different tissues of young and adult Dezhou donkeys, as well as association analysis of four gene polymorphic loci in 450 individuals. The results showed that expression levels of the ACSL1 gene are higher in heart, liver, spleen, lung, renal, gastric and muscle tissues of adult donkeys than in those of young donkeys. In the association analysis between genotype and body size traits, the wild genotype DD at the ACSL1-1 locus in female and male donkeys was greater than the mutant genotype II (P<0.05); genotype II of ACSL1-2 was significantly higher than that of DD in withers height, body length, rump width and body weight of male donkeys (P<0.05); and ACSL1-3 showed a tendency for the wild genotype II to be greater than the mutant genotype DD in female and male donkeys (P<0.05). In addition, among the five haplotype combinations constructed, Hap3Hap3 (II-II-DD-DD) and Hap6Hap6 (DD-II-II-II) haplotype combinations were superior to other haplotype combinations in growth traits, which also indicated that the results of haplotype combination association analysis and genotype association analysis tended to be the same. In conclusion, the results of this study indicate that the polymorphic loci of the ACSL1 gene can be used as candidate molecular markers for the growth and development of Dezhou donkeys, and provide a theoretical reference for the breeding of Dezhou donkeys.

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Multiple association analysis of loci and candidate genes that regulate body size at three growth stages in Simmental beef cattle

10.21203/rs.2.13744/v5 ◽

2020 ◽

Author(s):

Bingxing An ◽

Lei Xu ◽

Jiangwei Xia ◽

Xiaoqiao Wang ◽

Jian Miao ◽

...

Keyword(s):

Body Size ◽

Beef Cattle ◽

Candidate Genes ◽

Association Analysis ◽

Growth Traits ◽

Association Studies ◽

Growth Stages ◽

Genome Wide Association Studies ◽

Trait Analysis ◽

Multiple Association

Abstract Background: Body size traits as one of the main breeding selection criteria was widely used to monitor cattle growth and to evaluate the selection response. In this study, body size was defined as body height (BH), body length (BL), hip height (HH), heart size (HS), abdominal size (AS), and cannon bone size (CS). We performed genome-wide association studies (GWAS) of these traits over the course of three growth stages (6, 12 and 18 months after birth) using three statistical models, single-trait GWAS, multi-trait GWAS and LONG-GWAS. The Illumina Bovine HD 770K BeadChip was used to identify genomic single nucleotide polymorphisms (SNPs) in 1217 individuals. Results: In total, 19, 29, and 10 significant SNPs were identified by the three models, respectively. Among these, 21 genes were promising candidate genes, including SOX2, SNRPD1, RASGEF1B, EFNA5, PTBP1, SNX9, SV2C, PKDCC, SYNDIG1, AKR1E2, and PRIM2 identified by single-trait analysis; SLC37A1, LAP3, PCDH7, MANEA, and LHCGR identified by multi-trait analysis; and P2RY1, MPZL1, LINGO2, CMIP, and WSCD1 identified by LONG-GWAS. Conclusions: Multiple association analysis was performed for six growth traits at each growth stage. These findings offer valuable insights for the further investigation of potential genetic mechanism of growth traits in Simmental beef cattle.

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