Identification and relationship of old Japanese Hydrangea cultivars in Europe by morphological and nuclear SSR analysis

2019 ◽  
pp. 77-82
Author(s):  
H. Yamamoto ◽  
S. Yamamoto ◽  
S. Funahashi ◽  
M. Ogawa ◽  
M. Miyashita ◽  
...  
2021 ◽  
Vol 2 (1) ◽  
pp. 24-35
Author(s):  
Alexander Francis-Ratte

Abstract This paper presents an etymological analysis of the Japanese plural suffix tachi, Old Japanese tati. I propose that tati originates from a grammaticalization of an earlier Pre-Old Japanese phonological form *totwi, the non-bound reflex of which is the Old Japanese quasi-collective marker dwoti ‘fellow (person), everyone, together’. The reconstruction of a Pre-Old Japanese stem *totwi (Pre-Proto-Japanese /*tətəj/) with quasi-collective and plural function clarifies the possible connection of the Japanese plural suffix to the Korean plural suffix tul (Middle Korean tólh), which Whitman (1985, p. 217) proposed to be cognates but which has since been criticized on phonological and distributional grounds. I show that reconstructing the earliest form of the Japanese plural suffix as /*tətəj/ resolves each of the three phonological issues with the Japano-Koreanic comparison, creates a better morphosyntactic match between the two languages, and rules out a loanword relationship of the Japanese and Korean forms.


2020 ◽  
Vol 12 (5) ◽  
pp. 239
Author(s):  
Wenchao Li

This study tackles sequential voicing in Old Japanese with a focus on three matters: (a) the interaction of the eight vowels and aspirated consonants; (b) the association of the written system and sequential voicing; and (c) the interaction between the combinationality of each constituent and sequential voicing. Four hundred and seventy-two compound nouns of Old Japanese were collected from the corpus ‘The Japanese Lexicon: A Rendaku Encyclopedia’ by NINJAL. The findings reveal that (i) /k/ has the largest token number of sequential voicing and /p/ has the second largest token number, followed by /s/ and /t/; (ii) regarding the eight vowels /a/, /e1/, /e2/, /i1/, /i2/, /o1/, /o2/ and /u/, /a/ is most likely to form a [N1-N2] whose initial consonant is /k/, /p/ and /t/. It is not likely for the vowel /a/ to invite a voiced ‘/s/-initial’ N1’; /o1/ and /o2/ are both likely to combine with a voiced consonant /k/; /t/ and are less likely to yield a voiced /s/; /e1/ is more likely to invite a voiced consonant than /e2/, but /e1/ does not invite a voiced /p/; /e2/ does not yield a voiced /s/; and /i1/ is likely to take a voiced consonant than /i2/. /k/ and /t/ are the two consonants that are most likely to be voiced when forming a N-N with N1 ends with /i1/. /i2/ does not invite a voiced /p/, /s/ or /t/; /u/ never results in a voiced /s/; (iii) there is a split in the characters that renders a voiced phoneme or an unvoiced phoneme; and (iv) the semantic relationship of N1 and N2 in [N1-N2] that bears sequential voicing is of six types, of which the most frequent relationship of N1 and N2 is [Modifier - N2]. The [prefix-N2] construction is not subject to sequential voicing. 


Author(s):  
I. V. Stepanov ◽  
I. I. Suprun ◽  
D. M. Anatov ◽  
E. V. Lobodina ◽  
R. M. Osmanov

Genetic studies of diff erent geographical origin of apricot varieties contribute to the accumulation of information about distribution and cultivation history of this crop. Valuable material in such studies is the local autochthonous germplasm. The local Dagestan varieties can be included to representatives of the autochthonous germplasm of apricot. Genetic studies of the Dagestan germplasm will allow to evaluate its contribution in world apricot gene pool, as well as to determine its place among varieties with diff erent eco-geographical origin. Six SSR- markers previously elaborated on Siberian apricots were used in the genotyping of 12 apricot varieties. Much of the selected varieties were represented by local Dagestan forms. Microsatellite markers used in this study allowed to separate all varieties selected for work as genotypes with specifi c SSR profi les. The polymorphism level, expressed in the number of alleles per locus, had high values, based on the analysis of studies previously conducted in the world. In this regard, we can conclude that the involved SSR markers can be used in further studies aimed at analyzing the genetic diversity of the studied culture. In this study, clustering apricot varieties was used to determine related groups of varieties, based on the data SSR-genotyping. For cluster analysis the neighbor joining (NJ) method was used. The clustering of the samples carried out in the work, allowed us to reveal the intermediate position of Dagestan varieties relative to varieties of diff erent eco-geographical origin. To confi rm the obtained results, the PCoA main coordinates method which refl ecting the relationship of genotyped samples on the coordinate plane was additionally used. The patterns of samples distribution on the plot of coordinates revealed by PCoA generally corresponded to the clustering data using the neighbor joining (NJ). The study made it possible to evaluate the eff ectiveness of the SSR-markers used in the work for the genotyping of apricot varieties and to obtain information on the genetic relatedness of the local Dagestan varieties relative to other eco-geographical groups of varieties.


2018 ◽  
Vol 40 (4) ◽  
Author(s):  
Abdul Kareem ◽  
Muhammad Jafar Jaskani ◽  
Asim Mehmood ◽  
Iqrar Ahmad Khan ◽  
Faisal Saeed Awan ◽  
...  

Abstract Guava (Psidium guajava L.) is an open-pollinated crop having 25-40% dissimilarity index which promotes heterozygosity and adds new cultivars. Morpho-genetic characterization of 37 guava accessions was carried out for genetic variability and structure of guava germplasm located in Punjab province, Pakistan. Principal Component analysis (PCA) was subjected to analyze the morphological diversity and for genetic analysis we applied cluster analysis, using the PyElph software. PCA distributed thirty one traits into six components and first two components accounted 39.5% of total variation. A dendrogram constructed on the basis of morphological traits which showed 34% dissimilarity index among thirty seven guava accessions and divided them into 6 groups. For genetic characterization 18 microsatellites were used, the size of reproducible and scorable bands ranged from 150 to 320 bp. The 18 primer pairs amplified 85 alleles with an average number of 4.7 alleles per locus and no more than two displayed bands (nuclear SSR loci). The phylogenetic tree based on molecular analysis showed 50% dissimilarity index among selected guava accessions and separated them into 4 groups.


Paleobiology ◽  
1980 ◽  
Vol 6 (02) ◽  
pp. 146-160 ◽  
Author(s):  
William A. Oliver

The Mesozoic-Cenozoic coral Order Scleractinia has been suggested to have originated or evolved (1) by direct descent from the Paleozoic Order Rugosa or (2) by the development of a skeleton in members of one of the anemone groups that probably have existed throughout Phanerozoic time. In spite of much work on the subject, advocates of the direct descent hypothesis have failed to find convincing evidence of this relationship. Critical points are:(1) Rugosan septal insertion is serial; Scleractinian insertion is cyclic; no intermediate stages have been demonstrated. Apparent intermediates are Scleractinia having bilateral cyclic insertion or teratological Rugosa.(2) There is convincing evidence that the skeletons of many Rugosa were calcitic and none are known to be or to have been aragonitic. In contrast, the skeletons of all living Scleractinia are aragonitic and there is evidence that fossil Scleractinia were aragonitic also. The mineralogic difference is almost certainly due to intrinsic biologic factors.(3) No early Triassic corals of either group are known. This fact is not compelling (by itself) but is important in connection with points 1 and 2, because, given direct descent, both changes took place during this only stage in the history of the two groups in which there are no known corals.


Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.


Author(s):  
Leon Dmochowski

Electron microscopy has proved to be an invaluable discipline in studies on the relationship of viruses to the origin of leukemia, sarcoma, and other types of tumors in animals and man. The successful cell-free transmission of leukemia and sarcoma in mice, rats, hamsters, and cats, interpreted as due to a virus or viruses, was proved to be due to a virus on the basis of electron microscope studies. These studies demonstrated that all the types of neoplasia in animals of the species examined are produced by a virus of certain characteristic morphological properties similar, if not identical, in the mode of development in all types of neoplasia in animals, as shown in Fig. 1.


Author(s):  
J.R. Pfeiffer ◽  
J.C. Seagrave ◽  
C. Wofsy ◽  
J.M. Oliver

In RBL-2H3 rat leukemic mast cells, crosslinking IgE-receptor complexes with anti-IgE antibody leads to degranulation. Receptor crosslinking also stimulates the redistribution of receptors on the cell surface, a process that can be observed by labeling the anti-IgE with 15 nm protein A-gold particles as described in Stump et al. (1989), followed by back-scattered electron imaging (BEI) in the scanning electron microscope. We report that anti-IgE binding stimulates the redistribution of IgE-receptor complexes at 37“C from a dispersed topography (singlets and doublets; S/D) to distributions dominated sequentially by short chains, small clusters and large aggregates of crosslinked receptors. These patterns can be observed (Figure 1), quantified (Figure 2) and analyzed statistically. Cells incubated with 1 μg/ml anti-IgE, a concentration that stimulates maximum net secretion, redistribute receptors as far as chains and small clusters during a 15 min incubation period. At 3 and 10 μg/ml anti-IgE, net secretion is reduced and the majority of receptors redistribute rapidly into clusters and large aggregates.


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