scholarly journals Morpho-genetic profiling and phylogenetic relationship of guava (Psidium guajava l.) as genetic resources in Pakistan

2018 ◽  
Vol 40 (4) ◽  
Author(s):  
Abdul Kareem ◽  
Muhammad Jafar Jaskani ◽  
Asim Mehmood ◽  
Iqrar Ahmad Khan ◽  
Faisal Saeed Awan ◽  
...  

Abstract Guava (Psidium guajava L.) is an open-pollinated crop having 25-40% dissimilarity index which promotes heterozygosity and adds new cultivars. Morpho-genetic characterization of 37 guava accessions was carried out for genetic variability and structure of guava germplasm located in Punjab province, Pakistan. Principal Component analysis (PCA) was subjected to analyze the morphological diversity and for genetic analysis we applied cluster analysis, using the PyElph software. PCA distributed thirty one traits into six components and first two components accounted 39.5% of total variation. A dendrogram constructed on the basis of morphological traits which showed 34% dissimilarity index among thirty seven guava accessions and divided them into 6 groups. For genetic characterization 18 microsatellites were used, the size of reproducible and scorable bands ranged from 150 to 320 bp. The 18 primer pairs amplified 85 alleles with an average number of 4.7 alleles per locus and no more than two displayed bands (nuclear SSR loci). The phylogenetic tree based on molecular analysis showed 50% dissimilarity index among selected guava accessions and separated them into 4 groups.

OENO One ◽  
2005 ◽  
Vol 39 (3) ◽  
pp. 109
Author(s):  
Emmanuel D. Ladoukakis ◽  
François Lefort ◽  
Petraq Sotiri ◽  
Arjola Bacu ◽  
Efigjeni Kongjika ◽  
...  

<p style="text-align: justify;">A recently restored ampelographic collection of Albanian grapevine accessions has been submitted to genetic profiling with eleven nuclear microsatellite markers, widely used in other studies. Microsatellite profiling resulted in 28 single profiles for 29 accessions. Two cultivars, Shesh I bardhë and Pucalla, were found to be synonyms. Genetic profiles of Albanian cultivars were compared at 8 microsatellite loci to 29 most commonly cultivated Greek cultivars. Albanian cultivars were found to be more closely related to Greek cultivars from Peloponnese. One Greek cultivar named Dempina was found to be genetically close to two Albanian cultivars Debina teki and Debina kala, which are homonyms Another cultivar, known as Toska or Sinambel displayed a tri-allelic profile at 5 loci over 10 analysed loci. Such a high number of tri-allelic loci found in one individual favours the hypothesis of triploidy but the chimerism hypothesis cannot be excluded without further work.</p>


Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 533-546 ◽  
Author(s):  
Rodrigo Pérez-Sánchez ◽  
Remedios Morales-Corts ◽  
Ángeles Gómez-Sánchez

There are not many exhaustive works addressing the agromorphological characterization of traditional fig (Ficus carica L.) cultivars in Spain. In order to analyze the diversity of these fig genetic resources, twelve traditional fig cultivars from the Central-Western Region of Spain were surveyed and characterized agromorphologically. A total of forty descriptors, mainly defined by the International Plant Genetic Resources Institute and the International Union for the Protection of New Varieties of Plants, were used to describe the fruits, leaves and the tree itself over two consecutive years (2013-2014). Some of the cultivars showed distinctive and interesting agronomical characters from a commercial point of view, such as two crops per year (breba and fig), high yields, and fruit quality. This was the case of the fig cultivar widely distributed through the Duero river valley called ?Cuarter?n?. Its fruits were quite heavy and sweet (breba: 93.75 g and 25.91? Brix; fig: 42.41 g and 31.50? Brix), easy to peel, and juicy. Principal component analysis revealed that more than 67% of the agromorphological variability observed was explained by the first three components, some of the breba size parameters (fruit and neck length and fruit width) being the most important factors in differentiating the genotypes. A dendrogram clustered the cultivars into two major groups (unifera and bifera type) and revealed existing synonymies and homonymies. ?Carballar Negra? and ?Moscatel? were the only fig cultivars which did not have breba crops. This work is an important step in the conservation of genetic fig resources in Spain.


Hematology ◽  
2015 ◽  
Vol 2015 (1) ◽  
pp. 264-271 ◽  
Author(s):  
Kim-Hien T. Dao ◽  
Jeffrey W. Tyner

Abstract Atypical chronic myeloid leukemia (aCML) and chronic neutrophilic leukemia (CNL) are rare myeloid neoplasms defined largely by morphologic criteria. The discovery of CSF3R mutations in aCML and CNL have prompted a more comprehensive genetic profiling of these disorders. These studies have revealed aCML to be a genetically more heterogeneous disease than CNL, however, several groups have reported that SETBP1 and ASXL1 mutations occur at a high frequency and carry prognostic value in both diseases. We also report a novel finding—our study reveals a high frequency of U2AF1 mutations at codon Q157 associated with CSF3R mutant myeloid neoplasms. Collectively, these findings will refine the WHO diagnostic criteria of aCML and CNL and help us understand the genetic lesions and dysregulated signaling pathways contributing to disease development. Novel therapies that emerge from these genetic findings will need to be investigated in the setting of a clinical trial to determine the safety and efficacy of targeting various oncogenic drivers, such as JAK1/2 inhibition in CSF3R-T618I–positive aCML and CNL. In summary, recent advances in the genetic characterization of CNL and aCML are instrumental toward the development of new lines of therapy for these rare leukemias that lack an established standard of care and are historically associated with a poor prognosis.


Jurnal Jeumpa ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 545-553
Author(s):  
Aulia Agustina ◽  
Hasanuddin Hasanuddin

The study about “Phenetic Relationship of 7 Species of Orange Plants in BenerMeriah Highlands” was inducted back in August until September 2017. The aim of this study is to describe the morphological characterization of 7 species of orange plants in BenerMeriah highlands and to understand the phenetic relationship of 7 species of oranges plant in BenerMeriah highlands. The method used in this study was observation with the type of mixture between qualitative and quantitative. The subjects of this study are 7 species of orange plants, namely: a) Citrus reticulata; b) Citrus amblycarpa; c) Citrus hystrix; d) Citrus nobilis; e) Citrus maxima; f) Citrus sinensis; and g) Citrus aurantifolia. The parameters of this study were the morphological characteristic of stems, leaves, fruits and flowers. The collected data were analyzed descriptively, where the similarities were measured using Similarity Index (SI) and the dissimilarities were measured using Dissimilarity Index (DI) to classify the total of 7 species of orange plants using cluster analysis. According to the morphological characteristics, it could be concluded that there are several differences and similarities among the 7 species of orange plants found in BenerMeriah Regency. The combination of Citrus hystrix with Citrus aurantifolia have the closest phenetic relationship, which have 66 of SI. The other combinations that also have close phenetic relationship are Citrus hystrix with Citrus maxima and Citrus nobilis with Citrus sinensis. The pheneticrelationship that have the highest DI is the combination of Citrus amblycarpa with Citrus maxima, which have 88 of DI


2014 ◽  
Vol 61 (4) ◽  
pp. 829-839 ◽  
Author(s):  
V. Sitther ◽  
D. Zhang ◽  
D. L. Harris ◽  
A. K. Yadav ◽  
F. T. Zee ◽  
...  

2021 ◽  
Vol 12 (2) ◽  
pp. 372-378
Author(s):  
Njukeng Jetro Nkengafac ◽  
Ndille Claurence Nkumbe

This study was carried out to estimate leaf morphological diversity of some accessions/clones from IRRDB 1981 Hevea germplasm collection conserved at IRAD Ekona, to determine the importance of leaf morphological descriptors in differentiating accessions/clones. A total of 36 clones/ accessions were characterized using 6 leaf morphological descriptors. Analysis of variance showed that there were significant differences in the leaf morphological parameters for the studied clones. The Principal Component Analysis (PCA) showed that all leaf descriptors were informative and contributed significantly to the variation. The first 2 Principal Component scores (PCs) accounted for 88% of the total variation. The cluster analysis based on significant PCs grouped all accessions and clones in to 6 main clusters at the distance of 1.5. This study permits the characterization of Hevea accessions and clones in to diverse groups using leaf morphological descriptors; hence this will be advantageous for production of diverse genotypes during breeding programs to broaden the Hevea gene pool.


2007 ◽  
Vol 5 (03) ◽  
pp. 131-141 ◽  
Author(s):  
Sanjuana Hernández-Delgado ◽  
José Saúl Padilla-Ramírez ◽  
Alejandro Nava-Cedillo ◽  
Netzahualcoyotl Mayek-Pérez

Fifty morphological characteristics, fruit production over 3 years (from 1999 to 2002) and the amplified fragment length polymorphism (AFLP) technique were used to analyse a set of 48 guava (Psidium guajavaL.) accessions cultivated in Mexico, in order to characterize their genetic relationships. Germplasm was collected from the Calvillo-Cañones region and planted in Huanusco, Mexico. The study included twoP. cattleianum(Sabine) and twoP. friedrichsthalianum(Berg-Niedenzu) accessions from Costa Rica as outgroups. Principal component analysis (PCA) explained less than 30% of total variation and 14 characteristics from trees (1), leaves (2) and fruits (11) were the most informative. PCA analysis separated the germplasm into three major groups of accessions based on fruit size and weight, stem diameter and leaf size. Significant differences in fruit yield were detected among accessions and years, whereP.guajavaproduced 36 kg/year/tree of fresh fruit whileP. cattleianumandP. friedrichsthalianumshowed fruit yield lower than 7 kg/year/tree. The fruit yield broad sense heritability was 0.25. The AFLP analysis produced two clusters ofPsidiumaccessions, the first includedP. cattleianumandP. friedrichsthalianum, and the secondP. guajavaaccessions. This is the first report about the use of AFLP marker methodology for the genetic characterization of Mexican native guava germplasm and the results based on phenotypic and productive characteristics suggest that germplasm was selected from open pollinated trees.


Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.


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