Expression of Mal d 1 allergen genes in commercial apple cultivars

2021 ◽  
pp. 405-410
Author(s):  
T. Zunová ◽  
P. Vejl ◽  
M. Melounová ◽  
D. Čílová ◽  
R. Vávra
Keyword(s):  
Mal D 1 ◽  
Apple Cultivars

scholarly journals Relationship between Phenolic Compounds, Antioxidant Properties, and the Allergenic Protein Mal d 1 in Different Selenium-Biofortified Apple Cultivars (Malus domestica)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2647
Author(s):  
Sabrina Groth ◽  
Christoph Budke ◽  
Timo Weber ◽  
Susanne Neugart ◽  
Sven Brockmann ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Allergenic Protein ◽  
Antibody Recognition ◽  
Allergenic Potential ◽  
Allergenic Proteins ◽  
Other Substances ◽  
Mal D 1 ◽  
Apple Cultivars

Notable parts of the population in Europe suffer from allergies towards apples. To address this health problem, the analysis of the interactions of relevant allergens with other substances such as phenolic compounds is of particular importance. The aim of this study was to evaluate the correlations between the total phenolic content (TPC), polyphenol oxidase (PPO) activity, antioxidant activity (AOA), and the phenolic compound profile and the content of the allergenic protein Mal d 1 in six apple cultivars. It was found that the PPO activity and the content of individual phenolic compounds had an influence on the Mal d 1 content. With regard to the important constituents, flavan-3-ols and phenolic acids, it was found that apples with a higher content of chlorogenic acid and a low content of procyanidin trimers and/or epicatechin had a lower allergenic potential. This is probably based on the reaction of phenolic compounds (when oxidized by the endogenous PPO) with proteins, thus being able to change the conformation of the (allergenic) proteins, which further corresponds to a loss of antibody recognition. When apples were additionally biofortified with selenium, the composition of the apples, with regard to TPC, phenolic profile, AOA, and PPO, was significantly affected. Consequently, this innovative agronomic practice seems to be promising for reducing the allergenic potential of apples.

scholarly journals Investigation of Mal d 1 Allelic Variants and Phylogenetic Diversity in Contemporary and Historical Polish Apple Cultivars

2015 ◽  
Vol 7 (11) ◽  
Author(s):  
Kamila Lucia Bokszczanin ◽  
Andrzej Antoni Przybyla ◽  
Nicolas Krezdorn ◽  
Peter Winter ◽  
Marcin Filipecki
Keyword(s):  
Phylogenetic Diversity ◽  
Allelic Variants ◽  
Mal D 1 ◽  
Apple Cultivars

scholarly journals Molecular and Immunological Identification of Low Allergenic Fruits among Old and New Apple Varieties

2021 ◽  
Vol 22 (7) ◽  
pp. 3527
Author(s):  
Aleksandra Siekierzynska ◽  
Dorota Piasecka-Kwiatkowska ◽  
Wojciech Litwinczuk ◽  
Marta Burzynska ◽  
Aleksander Myszka ◽  
...  
Keyword(s):  
Real Time Pcr ◽  
Birch Pollen ◽  
Maturity Stage ◽  
Cultivation Method ◽  
Allergenic Potential ◽  
Mal D 1 ◽  
Apple Cultivars ◽  
Apple Fruits ◽  
Modern Varieties ◽  
Immunological Identification

About 50–70% of patients allergic to birch pollen suffer from sensitization after apple ingestion. Apple allergenicity was established in only few varieties. Studies were performed on apple fruits of 21 traditional and nine modern varieties organically, intensively, or integratively produced. The aim of the study was to assess whether the factors like cultivation method, maturity stage, genotype, or type of tissue place an impact on the allergenic potential of apples. To answer these questions, we used semiquantitative real-time PCR, ELISA, and immunoblotting. Apple allergen genes present divergent expression across apple cultivars. Expression of the Mal d 1.06A correlates with the Mal d 1 level and is affected by the cultivation method and maturity of the fruit. The content of the main allergen Mal d 1 varied widely across cultivars. Interestingly, in our study, the Gala variety presented a low Mal d 1 concentration regardless of the cultivation method. Based on the Mal d 1.06A expression, the Mal d 1 protein content, and the immunoreactivity assay, the Kandil Sinap, Kosztela, Rumianka from Alma-Ata, Kantówka Gdańska, Reinette Coulon, and Gala cultivars emerged as potentially hypoallergenic apple cultivars. Our study allowed distinguishing between potentially low, medium, and highly allergenic varieties.

RESULTS OF COOPERATION BETWEEN BREEDERS OF DIFFERENT INSTITUTIONS IN CREATION OF NEW GENERATION APPLE TREE

1970 ◽  
pp. 46-49
Author(s):  
Е. N. Sedov ◽  
T. V. Yanchuk ◽  
S. А. Korneeva ◽  
L. I. Dutova ◽  
Е. V. Ulianovskaya
Keyword(s):  
Selection Process ◽  
North Caucasus ◽  
Joint Work ◽  
Crop Breeding ◽  
Scientific Center ◽  
The North ◽  
State Register ◽  
Russian Research Institute ◽  
Apple Cultivars ◽  
New Generation

The experience in cooperation of breeders of different institutions in creating cultivars is shown. It is not always when the breeding institution has the necessary initial forms for selection. In this regard, there is a need to conduct separate stages of selection in different breeding institutions. For this purpose, a provision on authorship and continuity in the integrated work of several institutions in fruit breeding has been developed (Program and methods of fruit, berry and nut crop breeding. Annex. – Orel, 1995. – pp. 492-498). Breeding work of the Russian Research Institute of Fruit Crop Breeding (VNIISPK) and North Caucasian Federal Scientific Center of Horticulture, Viticulture, Winemaking can serve as a positive experience of creating new apple cultivars by two institutions. As a result of the joint work of these two institutions, 22 apple cultivars have been created, of which 9 have already been included in the state register of breeding achievements approved for use (zoned), including three cultivars for the conditions of the Middle zone of Russia – Aleksandr Boiko, Maslovskoye and Yablochny Spas and six cultivars for the conditions of the North Caucasus – Vasilisa, Karmen, Margo, Orfey, Soyuz and Talisman. Brief economical and biological characteristics of these cultivars are given in this paper. The obtained practical results indicate that in some cases, when creating modern cultivars that meet the requirements of production, it is necessary to use the knowledge of breeders, the source material and equipment of different breeding institutions, and carry out separate stages of the selection process in different institutions.

NEW VARIETIES OF FRUIT AND BERRIES IN SELECTION OF ALL-RUSSIAN RESEARCH INSTITUTE FOR NATURAL FOOD PRODUCTION

1970 ◽  
pp. 33-37
Author(s):  
N. S. Levgerova ◽  
Е. S. Salina ◽  
I. А. Sidorova
Keyword(s):  
Food Production ◽  
Raw Materials ◽  
Soluble Solids ◽  
Natural Food ◽  
Black Currant ◽  
Central Russia ◽  
Long Term Study ◽  
Apple Cultivars ◽  
Processed Products

The results of the technological assessment of new apple, cherry, black currant, red currant and gooseberry cultivars of VNIISPK breeding for the suitability for the natural food production are given. As a result, the cultivars that are promising for cultivation in raw plantings have been selected. For the production of raw materials in the juice industry, apple cultivars with a high juice yield and content of soluble solids higher than 10.0% were selected: ‘Bolotovskoye’ (Vf), ‘Candil Orlovsky’ (Vf), ‘Osipovskoye’ (3x), ‘Rozhdestvenskoye’ ((Vf + 3x), ‘Zaryanka’ (Vm), ‘Priokskoye’ ((Vf + Co), etc. Based on the long-term study of cultivars for their suitability for compote, jam and jelly, the cultivars that are most suitable for these types of processing are identified. It has been found that taking into account the daily needs of vitamins C and P as the most important antioxidants, all processed products from black currant can serve as their sources, all processed products from cherries, as well as apple juice and gooseberry marmalade can serve as a source of P-active compounds. All columnar apple cultivars as well as ‘Bolotovskoye’, ‘Rozhdestvenskoye’, ‘Veteran’, ‘Imrus’, etc. show high suitability for the production of apple chips. Cherry cultivars ‘Rovesnitsa’, ‘Putinka’, ‘Podarok Uchitelyam’ and ‘Novella’ are suitable for dried fruit. Based on the long-term studies of the technological qualities of the VNIISPK gene pool, a new generation assortment has been formed that has an optimal combination of chemical and technological indicators of fruits that meet modern technological requirements and are suitable for cultivation in the raw plantations of Central Russia.

Variation of Phytochemicals Content in Pulp and Skin of Seven Romanian Apple Cultivars

2017 ◽  
Vol 68 (3) ◽  
pp. 474-477
Author(s):  
Lacramioara Oprica ◽  
Doina Atofanei ◽  
Vladimir Poroch
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anthocyanin Content ◽  
Colour Intensity ◽  
Total Polyphenol ◽  
Valuable Source ◽  
Golden Delicious ◽  
Apple Cultivars ◽  
Apple Fruits

The amounts of anthocyanins, flavonoids, total polyphenol and ascorbic acid in seven apple cultivars from Romania were investigated. The amounts of polyphenol and flavonoids in pulp and skin of apple cultivars ranged between 17.18�7.52 mg GAE/g DW and 20.10�11.06 mg CE/g DW as well as 3.64�1.18 mg GAE/g DW and 10.31�5.57 mg CE/g DW, respectively. The highest and smallest values of ascorbic acid both in the skin and the pulp were observed in the cultivars Mutsu and Starkrimson. The anthocyanin content is positively correlated with the colour intensity of the apple epicarp, being about three-fold higher in Prima than Golden Delicious cultivar. The better antioxidant activity provided by the content of polyphenol, ascorbic acid and flavonoids was in apple fruits of Mutsu cultivar and for this reason it should be regarded as a valuable source of antioxidants.

Blossom Thinning in Pome and Stone Fruit

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 553b-553
Author(s):  
Esmaeil Fallahi
Keyword(s):  
Fruit Size ◽  
Naphthalene Acetic Acid ◽  
Full Bloom ◽  
Flower Bud ◽  
Hydrogen Cyanamide ◽  
Ammonium Thiosulfate ◽  
The Past ◽  
Apple Cultivars ◽  
Ortho Cresol ◽  
Bud Initiation

Early thinning of apples is important because of its impact on fruit size and next season's flower bud initiation. In the past, apple cultivars were often sprayed with the blossom thinner sodium dinitro-ortho-cresol(Elgetol) during full bloom, followed by a post-bloom application of a fruit thinner such as carbaryl with or without naphthalene acetic acid (NAA). Elgetol was removed from the market in 1989 because of the high cost of re-registration. Full-bloom sprays of sulfcarbamide (Wilthin), pelargonic acid (Thinex), and endothalic acid (Endothal), ammonium thiosulfate (ATS) or petal fall spray of carbaryl (Sevin XLR Plus) were developed as replacements for Elgetol. Hydrogen cyanamide (HC) and other chemicals have been used to eliminate or to reduce chilling requirements of peaches grown under the warm desert conditions. HC applied at “pink bloom” stage was observed to reduce the number of open blooms in `Florda Prince' peach; therefore, it was first used for blossom thinning in this cultivar in Arizona. Later, HC was also found to be an effective blossom thinner for plums in Idaho. HC has recently been found to effectively thin apple and peach blossoms. Armothin has also been an effective blossom thinner for peach in California.

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