Antimicrobial Effect of Aqueous and Ethanolic Extracts of Satureja Bachtiarica on Some Pathogenic Bacteria in Vitro

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

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Evaluation of antimicrobial effect of Commercial Norbixin pigment (Bixa orellana L.) against pathogenic bacteria and food spoilage in vitro

Food Science and Technology ◽

10.52547/fsct.18.119.133 ◽

2022 ◽

Vol 18 (119) ◽

pp. 133-142

Author(s):

Ahmad Nasrollahzadeh ◽

MAHMOUD REZAZAD ◽

ALMASI almasi ◽

MEHRAN moradi ◽

seyed mohamad ali ebrahimzade mousavi ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Antimicrobial Effect ◽

Bixa Orellana ◽

Food Spoilage

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Phytochemical Screening, Antibacterial and Synergistic activity of Dittrichia Viscosa Extracts against Multi-Resistant Pathogenic Bacteria

Pharmaceutics and Pharmacology Research ◽

10.31579/2693-7247/005 ◽

2019 ◽

Vol 2 (1) ◽

pp. 01-06

Author(s):

Rhazi Fouzia

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Antibacterial Effect ◽

Synergistic Activity ◽

Phytochemical Screening ◽

Bacterial Strains ◽

Dittrichia Viscosa ◽

Study Results ◽

Ethanolic Extracts

Study contextual: Faced with the global problem of antimicrobial resistance, and the use of traditional medicine for the research of antibacterial biomolecules. Aim: our work focused on the valorization of a medicinal plant Dittrichia viscosa which has many therapeutic and culinary virtues worldwide. Methods: To do this, a phytochemical screening of the leafy stems of the plant is carried out according to a set of physicochemical reactions, as well as an in vitro evaluation of the antibacterial activity, of the aqueous and ethanolic extracts against multi-resistant bacterial strains, by microdilution technique on microplates. An evaluation of the synergistic interaction between extracts and weakened antibiotics against pathogenic bacteria was also highlighted in this study. Results: The tests revealed the richness of Dittrichia viscosa species by tannins, flavonoids, saponosides, sterols and triterpenes. As for the antibacterial effect, the MICs range from 0.858±0.29 to 66.66 ± 0.00 mg / ml and the MBCs from 4.300 ± 1.01 to 11.610 ± 2.31 mg / ml is an interesting antibacterial activity. Regarding the combination of extracts with antibiotics tested, it revealed a synergistic action inducing an amplification of the antibacterial power of Penicillin, Imipenem and Erythromycin with a rate that reaches 471%. Conclusion: The results of this study show that the aqueous and ethanolic extracts of Dittrichia viscosa have interesting and promising antibacterial activity in the pharmaceutical, food and cosmetic industries.

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Evaluation of Antibacterial Activity of Some Traditionally Used Medicinal Plants against Human Pathogenic Bacteria

BioMed Research International ◽

10.1155/2015/265425 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 30

Author(s):

Bishnu P. Marasini ◽

Pankaj Baral ◽

Pratibha Aryal ◽

Kashi R. Ghimire ◽

Sanjiv Neupane ◽

...

Keyword(s):

Antibacterial Activity ◽

Medicinal Plants ◽

Inhibitory Action ◽

Pathogenic Bacteria ◽

Cynodon Dactylon ◽

Bacterial Species ◽

Dilution Method ◽

Chloroform Fraction ◽

Ethanolic Extracts

The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find thein vitroantibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range ofin vitrogrowth inhibitory action against the tested bacterial species, andCynodon dactylonwas found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistantStaphylococcus aureus, imipenem-resistantPseudomonas aeruginosa, multidrug-resistantSalmonella typhi, andS. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts ofCinnamomum camphora, Curculigo orchioides, andCurcuma longaexhibited the highest antibacterial activity againstS. pyogeneswith a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction ofCynodon dactylonexhibited best antibacterial activity againstS. aureuswith a MIC of 31 μg/mL. Among all,C. dactylon, C. camphora, C. orchioides, andC. longaplant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.

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In Vitro Cytotoxicity and Antibacterial Evaluation of Aqueous, Methanolic and Ethanolic Extracts of Anastatica hierochuntica against Pathogenic Bacteria

International Journal of Current Research in Biosciences and Plant Biology ◽

10.20546/ijcrbp.2016.306.002 ◽

2016 ◽

Vol 3 (6) ◽

pp. 14-22 ◽

Cited By ~ 2

Author(s):

Sanad M. Al Sobeai

Keyword(s):

Pathogenic Bacteria ◽

In Vitro Cytotoxicity ◽

Ethanolic Extracts ◽

Antibacterial Evaluation

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In Vitro Assessment for Antimicrobial Activity of Lactobacillus Helveticus and its Natural Glycopeptides Against Mastitis Causing Pathogens in Dairy Cat

The Open Biotechnology Journal ◽

10.2174/1874070701509010061 ◽

2015 ◽

Vol 9 (1) ◽

pp. 61-66 ◽

Cited By ~ 2

Author(s):

Gundega Gulbe ◽

Anda Valdovska ◽

Vaira Saulite ◽

Jevgenijs Jermolajevs

Keyword(s):

Antimicrobial Activity ◽

Lactic Acid ◽

Pathogenic Bacteria ◽

Antimicrobial Effect ◽

Lactobacillus Helveticus ◽

Haemolytic Activity ◽

Pathogenic Microorganisms ◽

Bacterial Strains ◽

Coagulase Negative Staphylococci

Probiotic lactic acid bacteria have a great potential to control bovine mastitis as well as they are favourable choice to treat many infectious diseases of human. These bacteria are well known as having many properties which make them beneficial to control pathogenic microorganisms. These include, the ability to adhere to cell, the reduction of pathogenic bacteria adherents, the co–aggregation, the production of organic acids, hydrogen peroxide, bacteriocin and etc., to be safe and non–pathogenic, which antagonize pathogenic microorganisms. However, each strain must be well identified and characterized in vitro before using for disease treatment. The aim of the present study was to screen three kind of test suspensions: TS1, TS2 and TS3, which contains probiotic lactic acid bacterium Lactobacillus helveticus or its natural glycopeptides, and other natural immunomodulators, in order to investigate which content were the most effective in inhibiting several mastitis causing bacteria in dairy cattle: coagulase–positive Staphylococcus aureus, coagulase–negative staphylococci S. haemolyticus, S. saprophyticus, S. simulans, S. vitulinus, and Gram–negative bacteria Citrobacter freundii and Serratia liquefaciens. Test suspensions TS1, TS2 and TS3 were adjusted by pH 6.3, then tested in vitro by well diffusion assay to determine their antimicrobial effect against bacteria. Furthermore haemolytic activity of applied test suspensions were determined. In results TS1 (9-13 mm) and TS2 (10-15 mm) showed the inhibition effect on four of eight tested bacterial strains, whereas TS3 did not displayed any antimicrobial effect. TS2 have a greatest antimicrobial activity as they resulted in the largest inhibition zones.

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Filtration effects of zebra mussels on pathogens and total bacterial burden in the Odra Lagoon (South Baltic)

Water Science & Technology ◽

10.2166/wst.2015.103 ◽

2015 ◽

Vol 71 (9) ◽

pp. 1354-1360

Author(s):

G. Daeschlein ◽

C. Fenske ◽

S. Scholz ◽

S. Dahlke ◽

M. Jünger ◽

...

Keyword(s):

Burkholderia Cepacia ◽

Dreissena Polymorpha ◽

Pathogenic Bacteria ◽

Bacterial Species ◽

Antimicrobial Effect ◽

Zebra Mussels ◽

Water Bodies ◽

North East ◽

Wastewater Pollution

As a result of their mode of filter feeding, zebra mussels (Dreissena polymorpha Pall.) have been observed to purify natural water bodies and in vitro. Therefore, the possibility of using zebra mussels for water purification was investigated in a slightly brackish water body of a large lagoon. In this study, water samples were taken above, near and at distance from zebra mussel beds (MB) in the Odra Lagoon in North East Germany. Near typical bacterial species like Aeromonas spp. pathogenic bacteria with potential relation to hospital wastewater pollution (Burkholderia cepacia, Staphylococcus aureus, Weeksella spp.) were detected. There were no correlations found between either total bacteria or pathogens and distance to MB and no antimicrobial effect of the mussels could be deduced. For bioremediation in larger water bodies like lagoons, natural zebra MB do not seem to play a major antimicrobial role and the effect of artificial mussel grids especially against hospital pathogens should be investigated.

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In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment

Frontiers in Pharmacology ◽

10.3389/fphar.2021.746808 ◽

2021 ◽

Vol 12 ◽

Author(s):

Tomas Kudera ◽

Barbora Fiserova ◽

Marie Korytakova ◽

Ivo Doskocil ◽

Hana Salmonova ◽

...

Keyword(s):

Cancer Cells ◽

Pathogenic Bacteria ◽

Folk Medicine ◽

Intestinal Bacteria ◽

Intestinal Cancer ◽

Inhibitory Effects ◽

Isolation And Identification ◽

Broth Microdilution Method ◽

Ethanolic Extracts

Bacterial diarrhea remains a global health problem, especially in developing tropical countries. Moreover, dysbiosis caused by diarrheagenic bacteria and inappropriate antimicrobial treatment has been associated with intestinal carcinogenesis. Despite the rich tradition of the use of herbs for the treatment of gastrointestinal disorders in Cambodian and Philippine folk medicine, many of them have not yet been systematically studied for their in vitro selective inhibitory effects on intestinal bacteria and cells. In the present study, in vitro inhibitory activities of 35 ethanolic extracts derived from 32 Cambodian and Philippine medicinal plants were determined by broth microdilution method against 12 pathogenic bacteria. Furthermore, cytotoxicity against intestinal cancer cells (Caco-2 and HT-29) using thiazolyl blue tetrazolium bromide cytotoxicity assay and safety to six beneficial intestinal bacteria (bifidobacteria and lactobacilli) and intestinal normal cells (FHs 74 Int) were determined for the antimicrobially active extracts. Selectivity indices (SIs) were calculated among the averages of minimum inhibitory concentrations (MICs), half-maximal inhibitory concentrations (IC50), and 80% inhibitory concentrations of proliferation (IC80) for each type of the tested agents. The extracts of Artocarpus blancoi (Elmer) Merr. (Moraceae), Ancistrocladus tectorius (Lour.) Merr. (Ancistrocladaceae), and Pentacme siamensis (Miq.) Kurz (Dipterocarpaceae) produced significant growth-inhibitory effects (MICs = 32–512 μg/ml) against intestinal pathogenic bacteria at the concentrations nontoxic to normal intestinal cells (IC80 values >512 μg/ml; SIs = 0.11–0.2). Moreover, the extract of P. siamensis (Miq.) Kurz was relatively safe to beneficial bacteria (MICs ≥512 μg/ml; SI = 0.1), and together with A. blancoi (Elmer) Merr., they selectively inhibited intestinal cancer cells (IC50 values ≥51.98 ± 19.79 μg/ml; SIs = 0.3 and 0.6). Finally, a strong selective antiproliferative effect on cancer cells (IC50 values 37.89 ± 2.68 to 130.89 ± 13.99 μg/ml; SIs = 0.5) was exerted by Ehretia microphylla Lam. (Boraginaceae), Lagerstroemia cochinchinensis Pierre ex Gagnep. (Lythraceae), and Melastoma saigonense (Kuntze) Merr. (Melastomataceae) (leaves with flower buds). The results suggest that the above-mentioned species are promising materials for the development of new selective antibacterial and antiproliferative agents for the treatment of infectious diarrhea and associated intestinal cancer diseases. However, further research is needed regarding the isolation and identification of their active constituents.

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ANTIMICROBIAL EFFECT OF Rosmarinus officinalis LINN ESSENTIAL OIL ON PATHOGENIC BACTERIA IN VITRO

Patologia: Doenças Bacterianas e Fúngicas ◽

10.22533/at.ed.99219180337 ◽

2019 ◽

pp. 281-295

Author(s):

Evalina Costa de Sousa ◽

Alexandra Barbosa da Silva ◽

Krain Santos de Melo ◽

Iriani Rodrigues Maldonade ◽

Eleuza Rodrigues Machado

Keyword(s):

Essential Oil ◽

Pathogenic Bacteria ◽

Antimicrobial Effect ◽

Rosmarinus Officinalis

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Selenium nanocomposites with polisakharidny matrixes stimulate growth of in vitro potatoes infected with the ring rot disease

Доклады Академии наук ◽

10.31857/s0869-56524893325-330 ◽

2019 ◽

Vol 489 (3) ◽

pp. 325-330 ◽

Cited By ~ 1

Author(s):

A. I. Perfileva ◽

O. A. Nozhkina ◽

I. A. Graskova ◽

A. V. Dyakova ◽

A. G. Pavlova ◽

...

Keyword(s):

Peroxidase Activity ◽

Pathogenic Bacteria ◽

Negative Impact ◽

Antimicrobial Effect ◽

Clavibacter Michiganensis ◽

Element Analysis ◽

Phytopathogenic Bacterium ◽

Ring Rot ◽

Rot Disease

In article for the first time investigated influence of nanocomposites of selenium in matrixes of an arabinogalaktan (Se/Ag) and starch (Se/St) on vegetation of in vitro plants potatoes, peroxidase activity and reactive oxygen species. Earlier it was shown that these nanocomposites of selenium have antimicrobial effect to a phytopathogenic bacterium of Clavibacter michiganensis ssp. sepedonicus (Cms). In this real work it is shown that the Se/Ag (6,4% of Se) and Se/St (12% of Se) have no negative impact on the healthy and infected with Cms potatoes, stimulating its growth, quantity of leaves and mass of a vegetative part. NC Se/Ag has a positive effect on potato plants by increasing its immune status by increasing ROS content and increasing peroxidase activity. With application of the element analysis it is shown that the studied nanocomposites do not collect in potatoes fabrics after its processing by them. The Se/Ag and Se/St as potential agents for improvement of potatoes from pathogenic bacteria allow to consider the received results.

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