scholarly journals Selenium nanocomposites with polisakharidny matrixes stimulate growth of in vitro potatoes infected with the ring rot disease

2019 ◽  
Vol 489 (3) ◽  
pp. 325-330 ◽  
Author(s):  
A. I. Perfileva ◽  
O. A. Nozhkina ◽  
I. A. Graskova ◽  
A. V. Dyakova ◽  
A. G. Pavlova ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
Pathogenic Bacteria ◽  
Negative Impact ◽  
Antimicrobial Effect ◽  
Clavibacter Michiganensis ◽  
Element Analysis ◽  
Phytopathogenic Bacterium ◽  
Ring Rot ◽  
Rot Disease

In article for the first time investigated influence of nanocomposites of selenium in matrixes of an arabinogalaktan (Se/Ag) and starch (Se/St) on vegetation of in vitro plants potatoes, peroxidase activity and reactive oxygen species. Earlier it was shown that these nanocomposites of selenium have antimicrobial effect to a phytopathogenic bacterium of Clavibacter michiganensis ssp. sepedonicus (Cms). In this real work it is shown that the Se/Ag (6,4% of Se) and Se/St (12% of Se) have no negative impact on the healthy and infected with Cms potatoes, stimulating its growth, quantity of leaves and mass of a vegetative part. NC Se/Ag has a positive effect on potato plants by increasing its immune status by increasing ROS content and increasing peroxidase activity. With application of the element analysis it is shown that the studied nanocomposites do not collect in potatoes fabrics after its processing by them. The Se/Ag and Se/St as potential agents for improvement of potatoes from pathogenic bacteria allow to consider the received results.

scholarly journals THE EFFECT OF NANOSCALE SELENIUM ON THE CAUSATIVE AGENT OF RING ROT AND POTATO IN VITRO

2019 ◽  
pp. 345-354 ◽  
Author(s):  
Irina Alekseyevna Graskova ◽  
Alla Innokent'yevna Perfileva ◽  
Ol'ga Aleksandrovna Nozhkina ◽  
Anastasiya Vyacheslavovna Dyakova ◽  
Vadim Nikolayevich Nurminsky ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Cultivated Plants ◽  
Clavibacter Michiganensis ◽  
Bacteriostatic Effect ◽  
Potato Plants ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Negative Effect ◽  
High Selenium

The biological activity of biopolymer-based selenium nanocomposite with a high selenium content of 6.4% was investigated. It was shown earlier that nanocomposites of selenium and arabinogalactan (NC Se/AG, 1.23% and 3.4% Se) have a bactericidal and bacteriostatic effect on the pathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) and do not adversely affect the vitality of potato plants in vitro. In this work, it was shown that the nanocomposite under scrutiny has a bacteriostatic effect, inhibits bacterial growth by 20% compared with the check variant and reduces the ability of Cms to form biofilms, which contribute to their high resistance to external factors. In the experiments conducted on plants, it was shown, there was not negative effect of NC Se/AG on biometric parameters, furthermore, this NC reduces the negative effect of potato infection with Cms. The nanocomposite reduced the activity of peroxidase and the content of reactive oxygen species in potato tissues. It was established that selenium does not accumulate in potato plants after the treatment with NC Se/AG. The results obtained allow us to consider NC Se/AG (6.4% Se) as an agent for healing cultivated plants from pathogenic bacteria.

THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

2018 ◽  
Vol 17 (6) ◽  
pp. 167-174 ◽  
Author(s):  
Małgorzata Schollenberger ◽  
Tomasz M. Staniek ◽  
Elżbieta Paduch-Cichal ◽  
Beata Dasiewicz ◽  
Agnieszka Gadomska-Gajadhur ◽  
...  
Keyword(s):  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Mentha Piperita ◽  
Mentha Spicata ◽  
Plant Essential Oils ◽  
Plant Pathogenic Bacteria ◽  
Interspecies Hybrids

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

scholarly journals Teknik Inokulasi Buatan Clavibacter michiganensis subsp. sepedonicus, Penyebab Penyakit Busuk Cincin Bakteri, pada Tanaman Kentang (Solanum tuberosum L.)

Agrikultura ◽  
2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Luciana Djaya ◽  
Ineu Sulastrini ◽  
Iin Rusita
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Block Design ◽  
Clavibacter Michiganensis ◽  
Bacterial Ring Rot ◽  
Seed Tubers ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Inoculation Techniques ◽  
Rot Disease

ABSTRACT Inoculation Techniques of Clavibacter michiganensis subsp. sepedonicus, the Cause of Bacterial Ring Rot Disease, on Potato (Solanum tuberosum L.). Clavibacter michiganensis subsp. sepedonicus, the cause of bacterial ring rot disease on potatoes, has been detected in potato fields in Pangalengan. To anticipate the spread of the pathogen, researches on the desease epidemiology are urgent to be carried out. Artificial inoculation techniques will be useful in the epidemiological studies. The objective of this reasearch was to evaluate some inoculation techniques, which are simple, cheap and fast in causing disease symptoms. The experiment was carried out at the laboratory and glasshouse of Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. The experiment was arranged in the randomized block design with five treatments of inoculation technique and five replications. The treatments were (a) soaking wounded seed tubers in pathogen suspension, (b) soaking not wounded seed tubers in pathogen suspension, (c) pathogen suspension was injected into leaf axil, (d) pathogen suspension was injected into seed tubers, and (e) pathogen suspension was poured into the planting holes. The results showed that stabbing and soaking tubers in pathogen suspension caused the shortest incubation period (17 days after inoculation) and the highest disease incidence (60%). Keywords : Clavibacter michiganensis subsp. sepedonicus, Bacterial ring rot, Potatoes, Inoculation techniquesABSTRAKClavibacter michiganensis subsp. sepedonicus (Cms), penyebab penyakit busuk cincin bakteri pada tanaman kentang, telah terdeteksi keberadaannya pada pertanaman kentang di Pangalengan. Dalam upaya mencegah penyebaran penyakit busuk cincin bakteri di Indonesia, perlu adanya studi epidemiologi patogen tersebut. Pada penelitian epidemiologi akan diperlukan cara menginokulasi tanaman secara buatan. Tujuan dari penelitian ini adalah untuk mendapatkan teknik inokulasi buatan yang dapat menyebabkan periode inkubasi yang lebih singkat dan persentase kejadian penyakit busuk cincin bakteri paling tinggi pada tanaman kentang. Percobaan dilaksanakan di rumah kaca dan laboratorium penyakit Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. Percobaan dirancang secara rancangan acak kelompok dengan lima perlakuan teknik inokulasi Cms dan lima ulangan, yaitu inokulasi dengan (a) merendam ubi benih yang telah dilukai dalam suspensi bakteri, (b) merendam ubi benih tanpa dilukai dalam suspensi bakteri, (c) suspensi bakteri ditusukkan pada ketiak daun tanaman kentang, (d) suspensi bakteri ditusukkan pada ubi benih, dan (e) suspensi bakteri disiramkan pada lubang tanam. Masing-masing ulangan terdiri dari lima tanaman. Hasil penelitian menunjukkan bahwa dari lima teknik inokulasi buatan yang dilakukan, inokulasi dengan melukai ubi benih dan merendamnya dalam suspensi patogen menghasilkanJurnal Agrikultura 2016, 27 (2): 66-71ISSN 0853-2885Teknik Inokulasi Buatan Clavibacter….67periode inkubasi tersingkat, yaitu 17 hari setelah inokulasi, dan persentase kejadian penyakit tertinggi yaitu sebesar 60%.Kata Kunci : Clavibacter michiganensis subsp. sepedonicus, Busuk cincin bakteri, Kentang, Inokulasi buatan

scholarly journals Evaluation of antimicrobial effect of Commercial Norbixin pigment (Bixa orellana L.) against pathogenic bacteria and food spoilage in vitro

2022 ◽  
Vol 18 (119) ◽  
pp. 133-142
Author(s):  
Ahmad Nasrollahzadeh ◽  
MAHMOUD REZAZAD ◽  
ALMASI almasi ◽  
MEHRAN moradi ◽  
seyed mohamad ali ebrahimzade mousavi ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Bixa Orellana ◽  
Food Spoilage

scholarly journals In Vitro Assessment for Antimicrobial Activity of Lactobacillus Helveticus and its Natural Glycopeptides Against Mastitis Causing Pathogens in Dairy Cat

2015 ◽  
Vol 9 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Gundega Gulbe ◽  
Anda Valdovska ◽  
Vaira Saulite ◽  
Jevgenijs Jermolajevs
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Lactobacillus Helveticus ◽  
Haemolytic Activity ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Coagulase Negative Staphylococci

Probiotic lactic acid bacteria have a great potential to control bovine mastitis as well as they are favourable choice to treat many infectious diseases of human. These bacteria are well known as having many properties which make them beneficial to control pathogenic microorganisms. These include, the ability to adhere to cell, the reduction of pathogenic bacteria adherents, the co–aggregation, the production of organic acids, hydrogen peroxide, bacteriocin and etc., to be safe and non–pathogenic, which antagonize pathogenic microorganisms. However, each strain must be well identified and characterized in vitro before using for disease treatment. The aim of the present study was to screen three kind of test suspensions: TS1, TS2 and TS3, which contains probiotic lactic acid bacterium Lactobacillus helveticus or its natural glycopeptides, and other natural immunomodulators, in order to investigate which content were the most effective in inhibiting several mastitis causing bacteria in dairy cattle: coagulase–positive Staphylococcus aureus, coagulase–negative staphylococci S. haemolyticus, S. saprophyticus, S. simulans, S. vitulinus, and Gram–negative bacteria Citrobacter freundii and Serratia liquefaciens. Test suspensions TS1, TS2 and TS3 were adjusted by pH 6.3, then tested in vitro by well diffusion assay to determine their antimicrobial effect against bacteria. Furthermore haemolytic activity of applied test suspensions were determined. In results TS1 (9-13 mm) and TS2 (10-15 mm) showed the inhibition effect on four of eight tested bacterial strains, whereas TS3 did not displayed any antimicrobial effect. TS2 have a greatest antimicrobial activity as they resulted in the largest inhibition zones.

Filtration effects of zebra mussels on pathogens and total bacterial burden in the Odra Lagoon (South Baltic)

2015 ◽  
Vol 71 (9) ◽  
pp. 1354-1360
Author(s):  
G. Daeschlein ◽  
C. Fenske ◽  
S. Scholz ◽  
S. Dahlke ◽  
M. Jünger ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Dreissena Polymorpha ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Antimicrobial Effect ◽  
Zebra Mussels ◽  
Water Bodies ◽  
North East ◽  
Wastewater Pollution

As a result of their mode of filter feeding, zebra mussels (Dreissena polymorpha Pall.) have been observed to purify natural water bodies and in vitro. Therefore, the possibility of using zebra mussels for water purification was investigated in a slightly brackish water body of a large lagoon. In this study, water samples were taken above, near and at distance from zebra mussel beds (MB) in the Odra Lagoon in North East Germany. Near typical bacterial species like Aeromonas spp. pathogenic bacteria with potential relation to hospital wastewater pollution (Burkholderia cepacia, Staphylococcus aureus, Weeksella spp.) were detected. There were no correlations found between either total bacteria or pathogens and distance to MB and no antimicrobial effect of the mussels could be deduced. For bioremediation in larger water bodies like lagoons, natural zebra MB do not seem to play a major antimicrobial role and the effect of artificial mussel grids especially against hospital pathogens should be investigated.

scholarly journals Anti-microbial Effect of Cocoa Leaf Extracts on Botryodiplodia theobromae; Leading Causative Organism of Crown Rot Disease of Banana (Musa acuminata)

2020 ◽  
pp. 40-50
Author(s):  
Patrick Kumah ◽  
Lartey Emmanuel Nunoo ◽  
Odum Bismark
Keyword(s):  
Antimicrobial Effect ◽  
Positive Control ◽  
Nutrient Broth ◽  
Crown Rot ◽  
Causative Organism ◽  
Microbial Count ◽  
Botryodiplodia Theobromae ◽  
Leaf Extracts ◽  
Rot Disease

An investigation was carried to assess in-vitro management of crown rot disease on Banana using extracts of cocoa leaves. The causative organism (Botryodiplodia theobromae) was sub cultured and the concentration determined through microbial count. Different cocoa leaves extracts were prepared using two different solvents. A conventional fungicide known as Mancozeb was used as a positive control and the nutrient broth only served as negative controls to help in assessment of the antimicrobial effect of the cocoa leave extracts. Phenolic Methanol Extracts (PME), Crude Cocoa Leaves Aqueous Extracts (CCAE) and Mancozeb were employed to prepare aqueous solutions through serial dilution with varied concentration. The nutrient broth (100 µL of double strength), 100 µL each of the plant extract concentrations, and 20 µL of inoculums were dispensed in a sterile micro-titre plate containing 96 wells. Incubation of the micro-titre plate was carried out at a temperature of 37°C for 1 day (24 hours). Susceptibility or otherwise of the microorganism (B. theobromae) from four sample stations was analyzed with 20 µL of a 5% solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT. The presence of blue-black wells indicated the growth of B. theobromae. The experiment was conducted in triplicates for PME (refined), CCAE and Mancozeb. The minimum inhibition concentrations (MICs) of PME (refined) that inhibited the growth of B. theobromae collected from four different locations were 5mg/ml, 2.5 mg/ml, 10 mg/ml and 2.5 mg/ml respectively for B. theobromae (B1)(Offinso), B. theobromae (B2)(Effiduase), B. theobromae (B4)(Kejetia) and B. theobromae (B5)(Ejisu) which was lower compared to the MICs of CCAE which was 30 mg/ml for the four sample stations. Comparatively, the MIC of the PME (5 mg/ml, 2.5 m g/ml, 10 mg/ml and 2.5mg/ml) was almost equivalent to the MIC of the conventional fungicide (Mancozeb) (2.5 mg/ml) for all the four sample stations of B. theobromae.

scholarly journals ANTIMICROBIAL EFFECT OF Rosmarinus officinalis LINN ESSENTIAL OIL ON PATHOGENIC BACTERIA IN VITRO

2019 ◽  
pp. 281-295
Author(s):  
Evalina Costa de Sousa ◽  
Alexandra Barbosa da Silva ◽  
Krain Santos de Melo ◽  
Iriani Rodrigues Maldonade ◽  
Eleuza Rodrigues Machado
Keyword(s):  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Rosmarinus Officinalis

Antimicrobial Effect of Herb Extracts against Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium Associated with Beef†

2000 ◽  
Vol 63 (5) ◽  
pp. 601-607 ◽  
Author(s):  
CATHERINE N. CUTTER
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Ground Beef ◽  
Antimicrobial Effect ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Herb Extracts

The effects of plant extracts against pathogenic bacteria in vitro are well known, yet few studies have addressed the effects of these compounds against pathogens associated with muscle foods. A series of experiments was conducted to determine the effectiveness of a commercially available, generally recognized as safe, herb extract dispersed in sodium citrate (Protecta One) or sodium chloride (Protecta Two) against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes associated with beef. In the first experiment, E. coli O157:H7, Salmonella Typhimurium, and L. monocytogenes inoculated onto beef and subjected to surface spray treatments with 2.5% solutions of Protecta One or Protecta Two were not affected by immediate application (day 0) of the herbal extracts. However, after 7 days of storage at 4°C, E. coli O157:H7 was reduced by >1.3 log10 CFU/cm2 by Protecta Two; L. monocytogenes was reduced by 1.8 and 1.9 log10 CFU/cm2 by Protecta One and Protecta Two, respectively; Salmonella Typhimurium was not reduced >0.3 log10 CFU/cm2 by either extract by day 7. In the second experiment, 2.5% Protecta Two (wt/vol or wt/wt) added to inoculated lean and adipose beef trim, processed, and packaged as ground beef chubs (80% lean, 20% adipose), did not reduce pathogen populations >0.5 log10 CFU/g up to 14 days at 4°C. In the third experiment, surface spray treatments of beef with 2.5% lactic acid or 2.5% solutions of Protecta One or Protecta Two, vacuum packaged, and stored up to 35 days at 4°C did reduce E. coli O157:H7, L. monocytogenes, and Salmonella Typhimurium slightly. These studies suggest that the use of herb extracts may afford some reductions of pathogens on beef surfaces; however, the antimicrobial activity may be diminished in ground beef by adipose components.

scholarly journals Categorisation of reactions and enumeration of bacteria in potato cultivars inoculated with the causal agent of bacterial ring rot

2018 ◽  
Vol 55 (No. 1) ◽  
pp. 11-22
Author(s):  
Pánková Iveta ◽  
Krejzar Václav ◽  
Krejzarová Radka
Keyword(s):  
Inoculum Size ◽  
Potato Cultivars ◽  
Clavibacter Michiganensis ◽  
Bacterial Ring Rot ◽  
Potato Plants ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Foliage Symptom ◽  
Symptom Group

Variability in the responses of plants propagated from in vitro tissue cultures of 52 ware and industrial potato cultivars to different Clavibacter michiganensis subsp. sepedonicus inoculum size was tested during 2015–2017. Bacterial ring rot symptoms on plants and tubers xylem vessels were recorded for 8 weeks and the susceptibility index (SI) for individual cultivars was calculated. Based on foliage symptoms, potato cultivars were placed into three symptoms groups. The symptomless group had SIs ≤ 1, for the moderate symptom group SIs ranged from 1.01 to 2.99, and the severe symptom group had SIs ≥ 3.0. The pathogen concentrations in vascular vessels of all infected potato plants increased during the experiment regardless of the foliage symptom group.

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